BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0025.p (208 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydcA [R] COG0705 Uncharacterized membrane protein (homolog of... 119 2e-27 BH0517 [R] COG0705 Uncharacterized membrane protein (homolog of ... 106 3e-23 BS_yqgP_1 [R] COG0705 Uncharacterized membrane protein (homolog ... 91 2e-18 BH1421_1 [R] COG0705 Uncharacterized membrane protein (homolog o... 81 1e-15 RSp1525 [R] COG0705 Uncharacterized membrane protein (homolog of... 73 4e-13 >BS_ydcA [R] COG0705 Uncharacterized membrane protein (homolog of Drosophila rhomboid) Length = 419 Score = 119 bits (299), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 11/209 (5%), Positives = 36/209 (17%), Gaps = 13/209 (6%) Query: 2 IFIRYEKWKGYIRYYPVTCILILLNVLMFIVLTANGGSTNL--ETLLKYGATLNAGPEMD 59 + + K + + L N+ ++ ++ Sbjct: 1 MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAKSGEDI----FGDGVLE 56 Query: 60 EMWRYVTATFLHNGFAHLFFNCFALLVFAPPLERLMGWWRYALLYMAGGFIANYLXXXXX 119 + + + + L + Sbjct: 57 ILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIRPPKEGER-----YF 111 Query: 120 XXXXXXXATVSVGASGAIYAVYGAFLYIALLQRGMMDENSRKTLYGLLVMGIIMSFATPY 179 + ++ + R M+ + T + + S Sbjct: 112 ALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTARVLDLASPIGRG 171 Query: 180 VNWMAHLSGLAAGFFLYGLIIRIFKRSRR 208 + A L I + + Sbjct: 172 QRGLIVAPPKAGKTMLLQNIAQ--SIAYN 198 >BH0517 [R] COG0705 Uncharacterized membrane protein (homolog of Drosophila rhomboid) Length = 419 Score = 106 bits (264), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 11/192 (5%), Positives = 34/192 (17%), Gaps = 9/192 (4%) Query: 2 IFIRYEKWKGYIRYYPVTCILILLNVLMFI-VLTANGGSTNLETLLKYGATLNAGPEMDE 60 + + K + + L N+ G + E Sbjct: 1 MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK----SGEDIFGDGVLE 56 Query: 61 MWRYVTATFLHNGFAHLFFNCFALLVFAPPLERLMGWWRYALLYMAGGFIANYLXXXXXX 120 + + ++L + + + + Sbjct: 57 ILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIRPPKEGE----RYFA 112 Query: 121 XXXXXXATVSVGASGAIYAVYGAFLYIALLQRGMMDENSRKTLYGLLVMGIIMSFATPYV 180 + ++ + R M+ + T + + S Sbjct: 113 LLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTARVLDLASPIGRGQ 172 Query: 181 NWMAHLSGLAAG 192 + A Sbjct: 173 RGLIVAPPKAGK 184 >BS_yqgP_1 [R] COG0705 Uncharacterized membrane protein (homolog of Drosophila rhomboid) Length = 419 Score = 90.5 bits (223), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 8/139 (5%), Positives = 24/139 (17%), Gaps = 13/139 (9%) Query: 19 TCILILLNVLMFIVLTANGGSTNLETLLKYGATLNAGPEMDEMWRYVTATFLHNGFAHLF 78 + + A + +L EM + + + A Sbjct: 180 PKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTEMQRLVKGEVVASTFDEPASRH 239 Query: 79 FNCFALLVFAPPLERLMGWWRYALLYMAGGF---IANYLXXXXXXXXXXXXATVSVGASG 135 +++ LL + + Sbjct: 240 VQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNTVVPASGK----------VLTGGV 289 Query: 136 AIYAVYGAFLYIALLQRGM 154 A++ + + Sbjct: 290 DANALHRPKRFFGAARNVE 308 >BH1421_1 [R] COG0705 Uncharacterized membrane protein (homolog of Drosophila rhomboid) Length = 419 Score = 81.3 bits (199), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 7/165 (4%), Positives = 27/165 (16%), Gaps = 7/165 (4%) Query: 22 LILLNVLMFIVLTANGGSTNLETLLKYGATLNAGPEMDEMWRYVTATFLHNGFAHLFFNC 81 + + + L G + + + + + Sbjct: 192 AQSIAYNHPDCVLMVLLIDERPEEVTEMQRLVKGEVVASTFDEPASRHVQVAEMVIEKAK 251 Query: 82 FALLVFAPPLERLMGWWRYALLYMAGGFIANYLXXXXXXXXXXXXATVSVGASGAIYAVY 141 + + L R A Y + ++ + Sbjct: 252 RLVEHKKDVIILLDSITRLARAYNTVVPASG-------KVLTGGVDANALHRPKRFFGAA 304 Query: 142 GAFLYIALLQRGMMDENSRKTLYGLLVMGIIMSFATPYVNWMAHL 186 L + ++ ++ + Sbjct: 305 RNVEEGGSLTIIATALIDTGSKMDEVIYEEFKGTGNMELHLSRKI 349 >RSp1525 [R] COG0705 Uncharacterized membrane protein (homolog of Drosophila rhomboid) Length = 419 Score = 72.8 bits (177), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 13/186 (6%), Positives = 30/186 (16%), Gaps = 14/186 (7%) Query: 18 VTCILILLNVLMFI---VLTANGGSTNLETLLKYGATLNAGPEMDEMWRYVTATFLHNGF 74 + V + + + + + + Sbjct: 207 LLIDERPEEVTEMQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDS 266 Query: 75 AHLFFNCFALLVFAPPLERLMGWWRYALLYMAGGFIANYLXXXXXXXXXXXXATVSVGAS 134 + +V A G AL F A ++ Sbjct: 267 ITRLARAYNTVVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLT-----IIATALI 321 Query: 135 GAIYAVYGAFLYIALLQ-RGMMDENSRK---TLYGLLVM-GIIMSFATPYVNWMAHLSGL 189 + M SRK + + + L + Sbjct: 322 DTGSKMDEVIY-EEFKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKM 380 Query: 190 AAGFFL 195 + Sbjct: 381 WILRKI 386 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.332 0.145 0.465 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 14,374,577 Number of extensions: 498269 Number of successful extensions: 1285 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 1273 Number of HSP's successfully gapped: 17 Length of query: 208 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 106 Effective length of database: 26,614,363 Effective search space: 2821122478 Effective search space used: 2821122478 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 113 (48.1 bits)