BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0019.p (249 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3832 [L] COG0847 DNA polymerase III, epsilon subunit and relat... 169 3e-42 BH1691_1 [L] COG0847 DNA polymerase III, epsilon subunit and rel... 89 4e-18 aq_932 [L] COG0847 DNA polymerase III, epsilon subunit and relat... 88 1e-17 BH3741 [L] COG0847 DNA polymerase III, epsilon subunit and relat... 86 4e-17 MT2247_1 [L] COG0847 DNA polymerase III, epsilon subunit and rel... 85 1e-16 >BH3832 [L] COG0847 DNA polymerase III, epsilon subunit and related 3'-5' exonucleases Length = 265 Score = 169 bits (429), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 88/216 (40%), Positives = 131/216 (60%), Gaps = 2/216 (0%) Query: 32 AQQTAQQMAFIRSLMREKRRPEVLHTPLSELETVIFDLETTGFSHQHGDEILSFGAXXXX 91 A +AQQ +++R L RE RR +VL PL +L V+FDLET+GF GD ILS GA Sbjct: 48 APSSAQQWSYVRQLQREIRRSDVLDLPLEKLPVVVFDLETSGFYPDQGDRILSIGAVKVV 107 Query: 92 XXXXXXXXCFYTLVNCQTSIPEDITKLTGISEEMTSAAPSLIDGLHNFMSFVGQQVLVAH 151 FY++V+ T PE+I +LTG+S E AAP L L +F +F+ LVAH Sbjct: 108 GLTIQEEETFYSVVHTDTVPPENILELTGLSVEELEAAPPLTSVLESFYAFIRHSTLVAH 167 Query: 152 GSNHDKSFLNAALWKTSKVQLTHRVLDTMMLARWLEPHRSNYTLDELLAVHGIPIQGRHN 211 ++H+K+F++ W+T K+ HRV+DT L + + P + TLD+ A + IP+Q RH+ Sbjct: 168 HASHEKAFMSHVTWQTMKLPFEHRVIDTSFLTKIVAP-QELVTLDDCCAYYSIPVQNRHH 226 Query: 212 ALEDARMTAQLWVHYLREISKKKQVDTLGDLYAYLS 247 A ED+ MTA+LW+ +++ +TL D+Y +L+ Sbjct: 227 AFEDSLMTAKLWI-AAIAAAREAGFETLRDIYVHLA 261 >BH1691_1 [L] COG0847 DNA polymerase III, epsilon subunit and related 3'-5' exonucleases Length = 226 Score = 89.4 bits (220), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 58/156 (37%), Positives = 77/156 (49%), Gaps = 2/156 (1%) Query: 65 VIFDLETTGFSHQHGDEILSFGAXXXXXXXXXXXXCFYTLVNCQTSIPEDITKLTGISEE 124 V+ D+ETTG S GD I+ GA C TLVN + SIP I +LTGIS+E Sbjct: 6 VVIDIETTGQSVAQGDRIIEIGAVVIEDEEAVESFC--TLVNPERSIPPFIEELTGISDE 63 Query: 125 MTSAAPSLIDGLHNFMSFVGQQVLVAHGSNHDKSFLNAALWKTSKVQLTHRVLDTMMLAR 184 M AP+ + MS + V VAH + D SFL L L ++DT+ L+R Sbjct: 64 MVKGAPTFHQCIPELMSMLEGGVFVAHNVHFDLSFLQKLLEHEGYGPLLVPIIDTVELSR 123 Query: 185 WLEPHRSNYTLDELLAVHGIPIQGRHNALEDARMTA 220 L P +Y L L G+ + H A DA++TA Sbjct: 124 ILLPTEESYKLSALTEQLGLEHENPHRADSDAKVTA 159 >aq_932 [L] COG0847 DNA polymerase III, epsilon subunit and related 3'-5' exonucleases Length = 202 Score = 88.2 bits (217), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 58/180 (32%), Positives = 86/180 (47%), Gaps = 6/180 (3%) Query: 65 VIFDLETTGFSHQHGDEILSFGAXXXXXXXXXXXXCFYTLVNCQTSIPEDITKLTGISEE 124 V+ DLE TGF + E++ A F TLV IPE I KLTGI+ Sbjct: 20 VVIDLEATGFDVEK-SEVIDLAAVRVEGGIITEK--FSTLVYPGYFIPERIKKLTGITNA 76 Query: 125 MTSAAPSLIDGLHNFMSFVGQQVLVAHGSNHDKSFLNAALWKTSKVQLTHRVLDTMMLAR 184 M P++ + L F+ FVG ++V H D F+N + + + L T+ LAR Sbjct: 77 MLVGQPTIEEVLPEFLEFVGDNIVVGHFVEQDIKFINKYTKQYRGKKFRNPSLCTLKLAR 136 Query: 185 WLEPHRSNYTLDELLAVHGIPIQGRHNALEDARMTAQLWVHYLREISKKKQVDTLGDLYA 244 + P Y+L E+ G G H AL+DA +TA++++ L E+ K + GD Y+ Sbjct: 137 KVFPGLKKYSLKEIAENFGFETNGVHRALKDATLTAEIFIKILEELWFKYGI---GDYYS 193 >BH3741 [L] COG0847 DNA polymerase III, epsilon subunit and related 3'-5' exonucleases Length = 219 Score = 86.3 bits (212), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 60/191 (31%), Positives = 90/191 (47%), Gaps = 5/191 (2%) Query: 55 LHTPLSELETVIFDLETTGFSHQHGDEILSFGAXXXXXXXXXXXXCFYTLVNCQTSIPED 114 L+TP+ +L +FD ETTGF+ GD ++ A F T VN + +IP + Sbjct: 18 LNTPIRQLAFTVFDTETTGFAVGAGDRLIEVAAVHVEGLEVTDF-TFQTYVNPERNIPAE 76 Query: 115 ITKLTGISEEMTSAAPSLIDGLHNFMSFV---GQQVLVAHGSNHDKSFLNAALWKTSKVQ 171 I +LTGISE+ AP+ + + +F FV G V H ++ D + L L + Sbjct: 77 IEELTGISEDKVKNAPASLPAIESFFRFVEQSGSAAWVGHYASFDVTVLKKELQRYQFSL 136 Query: 172 LTHRVLDTMMLARWLEPHRSNYTLDELLAVHGIPIQGRHNALEDARMTAQLWVHYLREIS 231 + DT+ L ++ P + L+ G I RH AL DA TA L+ LR + Sbjct: 137 NVPLLFDTLDLIGYISPSKDMRDLEVYAKQFGTYIYERHQALGDALTTAHLFCELLRHLE 196 Query: 232 KKKQVDTLGDL 242 + + TL DL Sbjct: 197 ARGKT-TLADL 206 >MT2247_1 [L] COG0847 DNA polymerase III, epsilon subunit and related 3'-5' exonucleases Length = 232 Score = 85.1 bits (209), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 72/198 (36%), Positives = 87/198 (43%), Gaps = 16/198 (8%) Query: 59 LSELETVIFDLETTGF-----SHQHGDEILSFGAXXXXXXXXXXXXCFYTLVNCQTSIPE 113 L E V+ DLETTG D I GA F TLVN Q SIP Sbjct: 30 LRETTFVVVDLETTGGRTTGNDATPPDAITEIGAVKVCGGAVLGE--FATLVNPQHSIPP 87 Query: 114 DITKLTGISEEMTSAAPSLIDGLHNFMSFVGQQVLVAHGSNHDKSFLNAALWKTSKVQLT 173 I +LTGI+ M AP++ L F F G VLVAH + D FL AA + Sbjct: 88 QIVRLTGITTAMVGNAPTIDAVLPMFFEFAGDSVLVAHNAGFDIGFLRAAARRCDITWPQ 147 Query: 174 HRVLDTMMLARWL-----EPHRSNYTLDELLAVHGIPIQGRHNALEDARMTAQLWVHYLR 228 +VL TM LAR + P L L AV P H AL+DAR T + +H L Sbjct: 148 PQVLCTMRLARRVLSRDEAPSVRLAALARLFAVASNPT---HRALDDARATVDV-LHALI 203 Query: 229 EISKKKQVDTLGDLYAYL 246 E + V T +L +YL Sbjct: 204 ERVGNQGVHTYAELRSYL 221 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.133 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,086,311 Number of extensions: 573363 Number of successful extensions: 987 Number of sequences better than 1.0e-05: 25 Number of HSP's gapped: 1001 Number of HSP's successfully gapped: 25 Length of query: 249 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 145 Effective length of database: 26,383,695 Effective search space: 3825635775 Effective search space used: 3825635775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)