BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0010.p (351 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value slr1418 [F] COG0167 Dihydroorotate dehydrogenase 307 2e-83 all4272 [F] COG0167 Dihydroorotate dehydrogenase 295 8e-80 PA3050 [F] COG0167 Dihydroorotate dehydrogenase 287 2e-77 SMc02245 [F] COG0167 Dihydroorotate dehydrogenase 279 4e-75 ZpyrD [F] COG0167 Dihydroorotate dehydrogenase 266 3e-71 >slr1418 [F] COG0167 Dihydroorotate dehydrogenase Length = 381 Score = 307 bits (786), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 169/363 (46%), Positives = 227/363 (62%), Gaps = 22/363 (6%) Query: 2 DPEKAHHLVIGGLNKSDLV--PGGSAAMRLMYGVPETAD--LAVDLFGLHFPTPVGLAAG 57 DPE+ H +I L K D + G + AM + AD L +GL+FP +GL+AG Sbjct: 19 DPERGHQRLIQTLKKVDTMHRNGFAVAMEQLEQAFTHADPRLQQTCWGLNFPNVLGLSAG 78 Query: 58 LDKNAEAVGGFSSIGFGFMEVGTVTPKGQPGNDSPRLFRLLPDEALINRMGFNNKGAEAM 117 DK EA + ++GFGF E+G VT QPGND PRLFRL D+A++NR+G NN+GA AM Sbjct: 79 CDKEGEAAAVWPALGFGFAELGAVTKYAQPGNDRPRLFRLPQDQAVLNRLGANNEGAVAM 138 Query: 118 AGRLKAIKK---RRIPVAVNIGRNKTTPNESAHEDYRQCIRTLYPYGDFFVVNISSPNTP 174 A +LK R IP+ +N+ ++K TP + A EDY +TL P D+FVVN+SSPNTP Sbjct: 139 AAKLKHTWDYYPRTIPIGINLCKSKITPLDQAVEDYVFSFQTLAPVADYFVVNVSSPNTP 198 Query: 175 DLRSLQHGSELSNLLAQVKEEMELQHQQSGAAKSLLVKIAPDVSDGELEYMVHTIAEAGM 234 LRSLQ EL + A ++ Q K LLVKI+PD+S + ++ + E + Sbjct: 199 GLRSLQESDELPRIFAGLQSANHWQ-------KPLLVKISPDLSWEAIAVIIDLVKEHNL 251 Query: 235 DGVIATNTTLSREGLQSD---KAG-----ETGGLSGKPLRDRSTEIISRIYRQTGGKLPI 286 G++ATNT+ R GL++ + G E GGLSG+P+RDR+TE+I IY+QTGG +PI Sbjct: 252 SGIVATNTSTRRSGLKTKILPQTGQPIEQEAGGLSGQPIRDRATEVIRYIYQQTGGTIPI 311 Query: 287 IGSGGIFTGQDAYDKIRAGASLVEIYTALIYEGPEVNRKVHAGLRKLLRRDGFSHILEAV 346 IG GGIFT +DA++KI+AGAS ++IYT IY GP V + GL + L GFSHI EAV Sbjct: 312 IGVGGIFTAEDAWEKIQAGASFLQIYTGWIYGGPWVVSDILQGLGEKLAAGGFSHISEAV 371 Query: 347 GAD 349 G D Sbjct: 372 GID 374 >all4272 [F] COG0167 Dihydroorotate dehydrogenase Length = 376 Score = 295 bits (755), Expect = 8e-80, Method: Compositional matrix adjust. Identities = 161/324 (49%), Positives = 213/324 (65%), Gaps = 16/324 (4%) Query: 35 ETADLAVDLFGLHFPTPVGLAAGLDKNAEAVGGFSSIGFGFMEVGTVTPKGQPGNDSPRL 94 E + L +LFGL FP PVGLAAG DK+ A +SS+GFGF E+GTVT GQPGN PRL Sbjct: 57 EDSRLEQNLFGLRFPNPVGLAAGFDKDGVAARIWSSLGFGFAELGTVTFVGQPGNPPPRL 116 Query: 95 FRLLPDEALINRMGFNNKGAEAMAGRLKAIK-KRRIPVAVNIGRNKTTPNESAHEDYRQC 153 FRL D+A +NRMGFNN GA MA RL K + IP+ +N+G++K TP E+A EDY Sbjct: 117 FRLPLDQAALNRMGFNNHGAAVMAARLADEKGQFSIPIGINLGKSKVTPLEAAAEDYLNS 176 Query: 154 IRTLYPYGDFFVVNISSPNTPDLRSLQHGSELSNLLAQVKEEMELQHQQSGAAKSLLVKI 213 R L GD+FVVN+SSPNTP LRSLQ S LS++L +++E + + K + VKI Sbjct: 177 FRLLKELGDYFVVNVSSPNTPGLRSLQDASMLSSILDVLQKE-------NNSHKPIFVKI 229 Query: 214 APDVSDGELEYMVHTIAEAGMDGVIATNTTLSREGL------QSDKA--GETGGLSGKPL 265 APD+ + ++ + G+IATNTT+ R+GL Q+ KA E GG+SG P+ Sbjct: 230 APDLEWEAIADIIGLAKTYQLAGIIATNTTIRRDGLKTQVIEQTGKAPQEEAGGISGAPV 289 Query: 266 RDRSTEIISRIYRQTGGKLPIIGSGGIFTGQDAYDKIRAGASLVEIYTALIYEGPEVNRK 325 RDRSTEII I++QT G++PIIG GGIFT +DA+ KI AGASL+++YT IY+GP + + Sbjct: 290 RDRSTEIIRFIWQQTQGEIPIIGVGGIFTPEDAWAKITAGASLIQVYTGWIYQGPMMVSQ 349 Query: 326 VHAGLRKLLRRDGFSHILEAVGAD 349 + GL L + I EA+G + Sbjct: 350 ILTGLLSKLEEHELNSISEAIGLE 373 >PA3050 [F] COG0167 Dihydroorotate dehydrogenase Length = 342 Score = 287 bits (734), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 162/340 (47%), Positives = 216/340 (63%), Gaps = 12/340 (3%) Query: 1 MDPEKAHHLVIGGLNKSDLV-PGGSAAM-RLMYGVPETADLAVDLFGLHFPTPVGLAAGL 58 + PE +H L I DL+ GG + RL+ P+ A L V + GL FP PVGLAAGL Sbjct: 12 LSPETSHELSI------DLIGAGGRLGLNRLL--TPKPASLPVSVLGLEFPNPVGLAAGL 63 Query: 59 DKNAEAVGGFSSIGFGFMEVGTVTPKGQPGNDSPRLFRLLPDEALINRMGFNNKGAEAMA 118 DKN +A+ GF +GFGF+E+GTVTP+ QPGN PRLFRL A+INRMGFNN G + + Sbjct: 64 DKNGDAIDGFGQLGFGFIEIGTVTPRPQPGNPRPRLFRLPQANAIINRMGFNNHGVDHLL 123 Query: 119 GRLKAIKKRRIPVAVNIGRNKTTPNESAHEDYRQCIRTLYPYGDFFVVNISSPNTPDLRS 178 R++A K R + + +NIG+N TP E A +DY C+ +Y + VN+SSPNTP LRS Sbjct: 124 ARVRAAKYRGV-LGINIGKNFDTPVERAVDDYLICLDKVYADASYVTVNVSSPNTPGLRS 182 Query: 179 LQHGSELSNLLAQVKEEMELQHQQSGAAKSLLVKIAPDVSDGELEYMVHTIAEAGMDGVI 238 LQ G L LL +++ E + G L +KIAPD++D E + + EAGMD VI Sbjct: 183 LQFGDSLKQLLEALRQRQEALALRHGRRVPLAIKIAPDMTDEETALVAAALVEAGMDAVI 242 Query: 239 ATNTTLSREGLQSDKAG-ETGGLSGKPLRDRSTEIISRIYRQTGGKLPIIGSGGIFTGQD 297 ATNTTL REG++ G E GGLSG P+R++ST + + + GG+LPII +GGI G Sbjct: 243 ATNTTLGREGVEGLPHGDEAGGLSGAPVREKSTHTVKVLAGELGGRLPIIAAGGITEGAH 302 Query: 298 AYDKIRAGASLVEIYTALIYEGPEVNRKVHAGLRKLLRRD 337 A +KI AGASLV+IY+ IY+GP + R+ + L RR+ Sbjct: 303 AAEKIAAGASLVQIYSGFIYKGPALIREAVDAIAALPRRN 342 >SMc02245 [F] COG0167 Dihydroorotate dehydrogenase Length = 362 Score = 279 bits (714), Expect = 4e-75, Method: Compositional matrix adjust. Identities = 157/343 (45%), Positives = 213/343 (62%), Gaps = 13/343 (3%) Query: 1 MDPEKAHHLVIGGLNKSDLVPGGSAAMRLMYGVPETADLAVDLFGLHFPTPVGLAAGLDK 60 DPE AH L I L KS LVP +A P L + GL F P+G+AAG DK Sbjct: 16 FDPEAAHGLSIKAL-KSGLVPSCAA--------PADPRLGQTVAGLVFSNPIGMAAGYDK 66 Query: 61 NAEAVGGFSSIGFGFMEVGTVTPKGQPGNDSPRLFRLLPDEALINRMGFNNKGAEAMAGR 120 NAE IGFGF E+GTVTP+ Q GND PRLFRL+ DEA+INR+GFNN+G A R Sbjct: 67 NAEVPEALLKIGFGFTEIGTVTPRPQAGNDKPRLFRLVEDEAVINRLGFNNEGHGAALAR 126 Query: 121 LKAIKKRRIPVAVNIGRNKTTPNESAHEDYRQCIRTLYPYGDFFVVNISSPNTPDLRSLQ 180 LKA + + + VNIG NK + + A DY IRT Y +F NISSPNTP LR LQ Sbjct: 127 LKACSREAL-IGVNIGANKDSADRIA--DYVTGIRTFYAVARYFTANISSPNTPGLRDLQ 183 Query: 181 HGSELSNLLAQVKEEMELQHQQSGAAKSLLVKIAPDVSDGELEYMVHTIAEAGMDGVIAT 240 LS LL+ V + + ++ G + +KIAPD+++ ++ + + G+DG+I + Sbjct: 184 ARESLSALLSAVLAARDDEARKGGRQVPVFLKIAPDLTEEGMDDIAAEVLAHGLDGLIVS 243 Query: 241 NTTLSREGLQSDK-AGETGGLSGKPLRDRSTEIISRIYRQTGGKLPIIGSGGIFTGQDAY 299 NTTLSREGL+ + A E GGLSGKPL ++ST +++R+ ++ G LPIIG GG+ + + A Sbjct: 244 NTTLSREGLKDRRQANEAGGLSGKPLFEKSTAVLARMRKRVGPHLPIIGVGGVCSAETAA 303 Query: 300 DKIRAGASLVEIYTALIYEGPEVNRKVHAGLRKLLRRDGFSHI 342 +KIRAGA LV++Y+ +IYEGP + ++ GL L R+ + I Sbjct: 304 EKIRAGADLVQLYSCMIYEGPGLPGRIVRGLSALCEREKLASI 346 >ZpyrD [F] COG0167 Dihydroorotate dehydrogenase Length = 336 Score = 266 bits (681), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 143/327 (43%), Positives = 201/327 (61%), Gaps = 8/327 (2%) Query: 1 MDPEKAHHLVIGGLNKSDLVPGGSAAMRLMYGVPETADLAVDLFGLHFPTPVGLAAGLDK 60 +DPE+AH L + P + + + P V+ GL F P+GLAAGLDK Sbjct: 13 LDPERAHEFTFQQLRRITGTPFEALVRQKVPAKP------VNCMGLTFKNPLGLAAGLDK 66 Query: 61 NAEAVGGFSSIGFGFMEVGTVTPKGQPGNDSPRLFRLLPDEALINRMGFNNKGAEAMAGR 120 + E + ++GFG +E+GTVTP+ QPGND PRLFRL+ E LINRMGFNN G + + Sbjct: 67 DGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFRLVDAEGLINRMGFNNLGVDNLVEN 126 Query: 121 LKAIKKRRIPVAVNIGRNKTTPNESAHEDYRQCIRTLYPYGDFFVVNISSPNTPDLRSLQ 180 +K + + +NIG+NK TP E +DY C+ +Y Y + +NISSPNTP LR+LQ Sbjct: 127 VKKAHYDGV-LGINIGKNKDTPVEQGKDDYLICMEKIYAYAGYIAINISSPNTPGLRTLQ 185 Query: 181 HGSELSNLLAQVKEEMELQHQQSGAAKSLLVKIAPDVSDGELEYMVHTIAEAGMDGVIAT 240 +G L +LL +K + + VKIAPD+S+ EL + ++ +DGVIAT Sbjct: 186 YGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKIAPDLSEEELIQVADSLVRHNIDGVIAT 245 Query: 241 NTTLSREGLQSDK-AGETGGLSGKPLRDRSTEIISRIYRQTGGKLPIIGSGGIFTGQDAY 299 NTTL R +Q K +TGGLSG+PL+ +STEII R+ + G+LPIIG GGI + A Sbjct: 246 NTTLDRSLVQGMKNCDQTGGLSGRPLQLKSTEIIRRLSLELNGRLPIIGVGGIDSVIAAR 305 Query: 300 DKIRAGASLVEIYTALIYEGPEVNRKV 326 +KI AGASLV+IY+ I++GP + +++ Sbjct: 306 EKIAAGASLVQIYSGFIFKGPPLIKEI 332 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,592,595 Number of extensions: 1226605 Number of successful extensions: 3243 Number of sequences better than 1.0e-05: 21 Number of HSP's gapped: 3210 Number of HSP's successfully gapped: 21 Length of query: 351 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 244 Effective length of database: 26,037,693 Effective search space: 6353197092 Effective search space used: 6353197092 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)