BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0005.p (266 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0298 [R] COG0561 Predicted hydrolases of the HAD superfamily 146 3e-35 lin0196 [R] COG0561 Predicted hydrolases of the HAD superfamily 135 6e-32 BH1746 [R] COG0561 Predicted hydrolases of the HAD superfamily 135 8e-32 VC1364 [R] COG0561 Predicted hydrolases of the HAD superfamily 132 6e-31 SPy1700 [R] COG0561 Predicted hydrolases of the HAD superfamily 128 1e-29 >lin0298 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 270 Score = 146 bits (369), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 99/282 (35%), Positives = 147/282 (52%), Gaps = 34/282 (12%) Query: 3 YKLIALDVDGTLLNDDHKLSPENREAIAEVTRQGGLIVLCTGR---SPQNSIPLMEEMGL 59 YKLIA+D+DGTLL DDHK++ E +EAI + +G +VLCTGR +N + +E Sbjct: 2 YKLIAIDIDGTLLTDDHKVTDEVKEAIRQAKLKGVKVVLCTGRPLVGVENYLTDLELREE 61 Query: 60 AGYVLGHNGAANVRVEDREVLHIYGLDARGLDPYIDYCRARDIH---------YDVNTAF 110 YV+ NGA +EV+ L L ++H Y N Sbjct: 62 GDYVISFNGAFVQDTYTKEVISHLTLGIDDLKEIYQVSLDSNLHMHFFDDKALYTPNREI 121 Query: 111 DMYVDNVANLTQEATYMYEHFRITPASLPAWEDFREPVVKFTVFTKADILDEA------- 163 Y A LT + +Y+ P EDF + +KA ++EA Sbjct: 122 GKYTIVEAYLTG-SQLIYKEIENVP------EDF--------IMSKAMFIEEAPELEAGI 166 Query: 164 QREWSTWTQQYNIVRSGEFFVDFMHPEASKGNALMNLASQLGIAQEEVLAIGNYYNDISM 223 + ++ ++Y++VRS F+++ ++ +ASKGNA+ L+ +LGI Q EV+ IG+ ND++M Sbjct: 167 AKMPESFREKYHLVRSTPFYLEILNRDASKGNAVKELSEKLGIKQSEVICIGDQENDVTM 226 Query: 224 LTYAGLGIAMDNSPLEVKAAADAVTGSNNDHGVRDALVKYCL 265 L +AGLGIAM N+P +K AD T SNND GV A+ K+ L Sbjct: 227 LEFAGLGIAMGNAPERIKQLADYTTASNNDSGVAKAIQKFVL 268 >lin0196 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 270 Score = 135 bits (340), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 86/266 (32%), Positives = 145/266 (54%), Gaps = 12/266 (4%) Query: 3 YKLIALDVDGTLLNDDHKLSPENREAIAEVTRQGGLIVLCTGR---SPQNSIPLMEEMGL 59 YK+IA+D+DGTLLND H+++P REAI ++G +VLCTGR + S+ ++ + + Sbjct: 2 YKIIAIDIDGTLLNDAHEITPAVREAIKAAKQKGVKVVLCTGRPLAGIKKSLIELDLLDI 61 Query: 60 AGYVLGHNGAANVRVEDREVLHIYGLDARGLDPYIDYCRARDIHYDVNTAFDMYVDN--V 117 Y + NGA + E L L+ L+ +C A+D++ +MYV + + Sbjct: 62 GDYAITFNGAVILETATEEPLADITLNKSELEEIYAFCHAQDVNVTYFDGKNMYVPSRKI 121 Query: 118 ANLTQEATYMYEHFRITPASLPAWEDFREPVVKFTVF---TKADILDEAQREWSTWTQQY 174 +T + + + TP E+ E + V T I D + T + + Sbjct: 122 TEITCQDALLLQ----TPLYHLPVEEAPESIHVSKVMLLDTPEKITDVIKNLPETIKENF 177 Query: 175 NIVRSGEFFVDFMHPEASKGNALMNLASQLGIAQEEVLAIGNYYNDISMLTYAGLGIAMD 234 +VRS + ++F+ +KG+AL L ++LGI+Q EV++IG+ NDI+M+ YAG+G+AM Sbjct: 178 YVVRSVPYNLEFLQKGVNKGSALELLVNKLGISQGEVMSIGDQENDITMIEYAGMGVAMG 237 Query: 235 NSPLEVKAAADAVTGSNNDHGVRDAL 260 N+ +K A+ T +NN+ GV +A+ Sbjct: 238 NATEHIKEIANYTTTTNNEDGVANAI 263 >BH1746 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 282 Score = 135 bits (339), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 98/281 (34%), Positives = 147/281 (52%), Gaps = 23/281 (8%) Query: 4 KLIALDVDGTLLNDDHKLSPENREAIAEVTRQGGLIVLCTGRS-PQNSIPLMEEMGLAGY 62 KLIA D+DGTLLND+ K+S EN EAI + G +V+ TGR + S+PL +E GL Sbjct: 2 KLIATDMDGTLLNDERKISKENAEAIKAAQQDGIHVVVATGRDFKEASMPL-KEAGLTCP 60 Query: 63 VLGHNGAANVRVEDREVLHIYGLDARGLDPYIDYCRARDIHYDVNTAFDMYVDN------ 116 +L NGA R + E++H L +D A D++++V T + D+ Sbjct: 61 ILCVNGAEE-RSANGELIHAVSLAKNEALTILDILTAADVYFEVYTNRGAFTDDYEKGLE 119 Query: 117 -VANLTQEATY--MYEHFR------ITPASLPAWEDFRE-----PVVKFTVFTKADILDE 162 V ++ Q A YE R ++ ED+ + V KF F++ + + Sbjct: 120 IVIDVLQSAGSDDSYEKMREIAKHRFESGAVQLTEDYTKLIDSTAVYKFLAFSRDETKRQ 179 Query: 163 AQREWSTWTQQYNIVRSGEFFVDFMHPEASKGNALMNLASQLGIAQEEVLAIGNYYNDIS 222 A ++ +++ S ++ H +A KG AL LA +LGI ++V+AIG+ ND+S Sbjct: 180 AAKDDLEKKDVFSVSASASDNLEITHWQAQKGIALRRLAERLGINSDDVMAIGDNLNDVS 239 Query: 223 MLTYAGLGIAMDNSPLEVKAAADAVTGSNNDHGVRDALVKY 263 ML YAG +AM+N EVK AD VT SN D+GV A+ K+ Sbjct: 240 MLKYAGTAVAMENGEAEVKQLADFVTASNEDNGVAKAINKW 280 >VC1364 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 273 Score = 132 bits (332), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 90/274 (32%), Positives = 144/274 (52%), Gaps = 19/274 (6%) Query: 3 YKLIALDVDGTLLNDDHKLSPENREAIAEVTRQGGLIVLCTGRSPQNSIPLMEEMGLAG- 61 YKLIALD+DGTLLN ++SP ++AIA+ +QG +VL +GR +EE+ L Sbjct: 2 YKLIALDMDGTLLNSQKQISPRTKQAIAQARQQGIQVVLASGRPLDGMRSKLEELHLTTE 61 Query: 62 --YVLGHNGAANVRVEDREVLHIYGLDARGLDPYIDYCRARDIHYDVNT-AFDMYVDNVA 118 YVL +NG+ V E++H +D + + + +NT AF + Sbjct: 62 NDYVLYYNGSMVANVGTGEIIHQQIIDGKAAKRVATLAQ----EFGLNTHAFS----QIH 113 Query: 119 NLTQEATYMYEHFRITPASLPAWE-DFRE-----PVVKFTVFTKADILDEAQREW-STWT 171 L T Y + L E DF + P++K + + + L +A + + W Sbjct: 114 GLITPKTSQYTEVEASINGLNITEMDFAQLADDHPIIKAMIVGEPEKLTQAIAQLPAQWR 173 Query: 172 QQYNIVRSGEFFVDFMHPEASKGNALMNLASQLGIAQEEVLAIGNYYNDISMLTYAGLGI 231 +++ +V+S FF++F++ ++KG + +A+ LGI EV+ +G+ ND ML YAGLGI Sbjct: 174 EEFTVVQSAPFFLEFLNTGSNKGIGVQAIAAHLGIQASEVICMGDAENDHHMLQYAGLGI 233 Query: 232 AMDNSPLEVKAAADAVTGSNNDHGVRDALVKYCL 265 AM N+ E K AD +T SN+ GV A+ ++ L Sbjct: 234 AMANAMEETKHIADHITLSNDHDGVAVAIERFAL 267 >SPy1700 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 274 Score = 128 bits (321), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 86/268 (32%), Positives = 142/268 (52%), Gaps = 7/268 (2%) Query: 4 KLIALDVDGTLLNDDHKLSPENREAIAEVTRQGGLIVLCTGRSPQNSIPLMEEMGLAG-- 61 +LIA+D+DGTLLN D ++ EN AI E + G IVLCTGR + P +++GL Sbjct: 3 QLIAIDLDGTLLNQDKQIPKENITAIQEAAQSGLKIVLCTGRPQSGTRPYFDQLGLTQEE 62 Query: 62 YVLGHNGAANVRVEDREVLHIYGLDARGLDPYIDYCRA-RDIHYDVNTAFDMYV--DNVA 118 +++ +NG + D ++ H L ++ + ++ DI+ + D V + V Sbjct: 63 FLIINNGCSTYSSPDWQLRHSKMLKVSDIELLEELSQSFPDIYLTLTEENDYLVLEEEVP 122 Query: 119 NLTQEATYMYEHFRITPASLPAWEDFREPVVKFTVFTKADILDEAQRE-WSTWTQQYNIV 177 +L QE + + P SL D + + + LD +R + +Q +++V Sbjct: 123 DLVQEDGDLVFTI-VKPVSLAELSDTPRLIFQAMYLGEKAALDAFERAVRNQLSQSFHVV 181 Query: 178 RSGEFFVDFMHPEASKGNALMNLASQLGIAQEEVLAIGNYYNDISMLTYAGLGIAMDNSP 237 RS + ++ + SK +AL L LG+ ++V+AIG+ NDI MLTYAGLG+AM+N+ Sbjct: 182 RSQDNILEILPQGVSKASALKELVEDLGLTADQVMAIGDAPNDIEMLTYAGLGVAMENAS 241 Query: 238 LEVKAAADAVTGSNNDHGVRDALVKYCL 265 +K AD VT +N+ GV A+ ++ L Sbjct: 242 AAIKPLADKVTLTNDMAGVAQAIRQFAL 269 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,326,641 Number of extensions: 809696 Number of successful extensions: 2205 Number of sequences better than 1.0e-05: 90 Number of HSP's gapped: 2156 Number of HSP's successfully gapped: 97 Length of query: 266 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 161 Effective length of database: 26,268,361 Effective search space: 4229206121 Effective search space used: 4229206121 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)