BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0002.p (508 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA2363 [L] COG0420 DNA repair exonuclease 287 3e-77 BS_yhaO [L] COG0420 DNA repair exonuclease 214 4e-55 lin2325 [L] COG0420 DNA repair exonuclease 208 1e-53 SA1662 [L] COG0420 DNA repair exonuclease 165 2e-40 >MA2363 [L] COG0420 DNA repair exonuclease Length = 673 Score = 287 bits (735), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 22/421 (5%), Positives = 69/421 (16%), Gaps = 10/421 (2%) Query: 6 FLHAADLHLDSRFAGLAQLPQAIRSYLRESTFAALGRLVSVAVQEKTDFVVISGDVYDIS 65 + ++ A L + ++ TF +T + + + Sbjct: 24 AGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMIST 83 Query: 66 DASLQGQLRFQEALQELGKHGIAVFLIHGNHDPLDGPRLSTEPPGHVTVFGGSEPGQAVA 125 +L + +E K ++F L V + Sbjct: 84 FHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLIS--TISN 141 Query: 126 RRRSDGREVAVVSGISYPTAKVTENTALQFKRRPGSDLYHIALLHGNVDGDPQHETYSPC 185 + + ++ + + + + Sbjct: 142 WKNDLKTPSQAAASAIGERDRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQANEEVR 201 Query: 186 SRKDLIGRGYDYWALGHIHKRRVLHEQPPIV-YPGNIQGRSVKETGPKGCYVVDVNEAGQ 244 R R + + + + ++ N Sbjct: 202 KRWQNKIRYLLVDEYQDTNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLL 261 Query: 245 ATLKFHELDAVRWHVRELSIEGLADEAQWAGAVEQAVEEVRDAFPELMSVVRFRLTGRGA 304 + S L E+ + + ++ Sbjct: 262 SQDFPALKVIKLEQNYRSSGRILKAANILIANNPHVFEKRLFSELGYGAELKVLSANNEE 321 Query: 305 VHKALAEKGAAEDLLAELQRREAMRAERKAYAGLVWTEGFALETGLAVDRGRLVQEDSFL 364 + A + + + Sbjct: 322 HEAERVTGELIAHHFVNK-TQYKDYAIL-----YRGNHQSRVFEKFLMQNRIPYKISGGT 375 Query: 365 GEMLRLAGRSEQSSAGLDELMDSALKPLMESRELRRMLASVTPEEKLGWLRNAAELGISL 424 R + + + + + +KLG + Sbjct: 376 SFFSRPEIKDLLAYLRVLTN-PDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFT 434 Query: 425 L 425 Sbjct: 435 A 435 >BS_yhaO [L] COG0420 DNA repair exonuclease Length = 673 Score = 214 bits (544), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 22/316 (6%), Positives = 50/316 (15%), Gaps = 11/316 (3%) Query: 6 FLHAADLHLDSRFAGLAQLPQAIRSYLRESTFAALGRLVSVAVQEKTDFVVISGDVYDIS 65 A + R + G F + Sbjct: 7 QQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAREMKER 66 Query: 66 DASLQGQLRFQEALQELGKHGIAVFLIHGNHDPLDGPRLSTEPPGHVTVFGGSEPGQAVA 125 G+ + + + S + Sbjct: 67 VGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKELTEGLIE 126 Query: 126 RRRSDGREVAVVSGISYPTAKVTENTALQFKRRPGSDLYHIALLHGNVDGDPQHETYSPC 185 ++S IS + + D A +G D + Sbjct: 127 DD--KVLLQQLISTISNWKNDLKTPSQAAASAIGERD-RIFAHCYGLYDAHLKACNVLDF 183 Query: 186 SRKDLIGRGYDYWALGHIHKRRVLH-EQPPIVYPGNIQGRSVKETGPKGCYVVDVNEAGQ 244 L+ + + Y + G G Sbjct: 184 DDLILLPTLLLQANEEVRKRWQNKIRYLLVDEYQDTNTSQYELVKLLVGSRARFTVV-GD 242 Query: 245 ATLKFHELDAVRWHVRELSIEGLADEAQWAGAVEQAVEEVRDAFPELMSVVRFRLTGRGA 304 + R L + + ++ + + Sbjct: 243 DDQSIYSWRGARPQNLVLLSQ----DFPALKVIKLEQNYRSSGRILKAANILIANNPHVF 298 Query: 305 VHKALAE--KGAAEDL 318 + +E GA + Sbjct: 299 EKRLFSELGYGAELKV 314 >lin2325 [L] COG0420 DNA repair exonuclease Length = 673 Score = 208 bits (530), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 20/258 (7%), Positives = 39/258 (15%), Gaps = 6/258 (2%) Query: 1 MIPFRFLHAADLHLDSRFAGLAQLPQAIRSYLRESTFAALGRLVSVAVQEKTDFVVISGD 60 M A + LA + + A Sbjct: 1 MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAA 60 Query: 61 VYDISDASLQGQLRFQEALQELGKHGIAVFLIHGNHDPLDGPRLSTEPPGHVTVFGGSEP 120 + L H + + +I + L + + Sbjct: 61 REMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLA--LLK 118 Query: 121 GQAVARRRSDGREVAVVSGISYPTAKVTENTALQFKRRPGSDLYHIALLHGN-VDGDPQH 179 D + + K T Q + H + Sbjct: 119 ELTEGLIEDDKV-LLQQLISTISNWKNDLKTPSQAAASAIGE-RDRIFAHCYGLYDAHLK 176 Query: 180 ETYSPCSRKDLIGRGYDYWALGHIHKRRVLHEQPPIVYPGNIQGRSVKETGPKGCYVVDV 239 ++ A + KR + +V S E Sbjct: 177 ACNVLDFDDLILLPTLLLQANEEVRKRWQNKIRYLLVDEYQDTNTSQYELVKLLVGSRAR 236 Query: 240 NEAGQATLKFHELDAVRW 257 Sbjct: 237 F-TVVGDDDQSIYSWRGA 253 >SA1662 [L] COG0420 DNA repair exonuclease Length = 398 Score = 165 bits (417), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 96/299 (32%), Positives = 157/299 (52%), Gaps = 17/299 (5%) Query: 5 RFLHAADLHLDSRFAGLAQLPQAIRSYLRESTFAALGRLVSVAVQEKTDFVVISGDVYDI 64 +F+H +DLHLDS F + + I +++S + + +V +A+Q+ DFV+I+GD++D Sbjct: 3 KFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDIALQQDVDFVIIAGDLFDS 62 Query: 65 SDASLQGQLRFQEALQELGKHGIAVFLIHGNHDPLDGPRLSTEPPGHVTVFGGS-EPGQA 123 + +L+ ++ ++ + L I V++ HGNHDPL ++S+ P +V+VF E +A Sbjct: 63 ENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPL-SSKISSNWPDNVSVFSNKVETYEA 121 Query: 124 VARRRSDGREVAVVSGISYPTAKVTENTALQFKRRPGSDLYHIALLHGNVDGDPQHETYS 183 + + E + G SY EN ++ G HI +LHG +E Y+ Sbjct: 122 ITK----SGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHIGVLHGTYSKSSVNERYT 177 Query: 184 PCSRKDLIGRGYDYWALGHIHKRRVLHEQPPIVYPGNIQGRSVKETGPKGCYVVDVNEAG 243 +DL + Y YWALGHIH+R+ L + P I Y GNIQGR E G KGC +++ + Sbjct: 178 EFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGRHFNEQGEKGCLLIEGDHLK 237 Query: 244 QATLKFHELDAVRWHVRELSIEGLADEAQWAG---AVEQAVEEVRD---AFPELMSVVR 296 T KF+ +R+ E +IE D+ G ++ E+VR+ AF L V+ Sbjct: 238 LKT-KFYPTQYIRF--EEATIE--TDKTSKQGLYEVIQNFKEQVREEGKAFYRLTLVIN 291 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 37,810,177 Number of extensions: 1648138 Number of successful extensions: 5362 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 5356 Number of HSP's successfully gapped: 4 Length of query: 508 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 397 Effective length of database: 25,576,357 Effective search space: 10153813729 Effective search space used: 10153813729 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 118 (50.1 bits)