BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0342.0040.p (561 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ctpA [M] COG0793 Periplasmic protease 64 1e-09 all2500 [M] COG0793 Periplasmic protease 62 3e-09 BS_yvjB [M] COG0793 Periplasmic protease 59 2e-08 CC3435 [M] COG0793 Periplasmic protease 53 2e-06 >BS_ctpA [M] COG0793 Periplasmic protease Length = 466 Score = 63.5 bits (153), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 79/295 (26%), Positives = 123/295 (41%), Gaps = 51/295 (17%) Query: 218 GVGISTVQLDDGKVLVSLLIPGSPADQYGIKLGAEIISWDGKDVKKALDETSWSENPPAT 277 G+G + V+ DG++L+ I GSPA++ GIK +II +GK VK + +E Sbjct: 105 GIG-AQVEEKDGEILIVSPIKGSPAEKAGIKPRDQIIKVNGKSVKGM----NVNEAVALI 159 Query: 278 EGHRILNQGRFLARAPIGQNIQVKFRNQDDPAIITAALTAYDDQYEXXXXXXXXXXXEDP 337 G + L RA +G NI + + P Sbjct: 160 RGKKGTKVKLELNRAGVG-NIDLSIKRDTIPV---------------------------E 191 Query: 338 PVEGQILKENYGYVKIRYFLPGATQSDPAKALEDTIKEFQEKQVKGIIIDLRDNPGGDDD 397 V ++ N G ++I F AK L D I ++K KG I+DLR NPGG + Sbjct: 192 TVYSEMKDNNIGEIQITSF-----SETTAKELTDAIDSLEKKGAKGYILDLRGNPGGLME 246 Query: 398 LVAQMAGHFVNRKRIFEYVSYYNRNTGRFEINRAETRTVQPSRIYFPGKIAVLINHNTAS 457 M+ F+++ + V Y N G E+ +AE + ++ P VL+N TAS Sbjct: 247 QAITMSNLFIDKGKNIMQVEYKN---GSKEVMKAE----KERKVTKP--TVVLVNDGTAS 297 Query: 458 SGEGLPLILKGMPNVKIIGFTSTNGSFGIVSAPIEVKMPEGYVVQFPDGRSLNLD 512 + E + L NV +IG T +FG + + +G V+ + L D Sbjct: 298 AAEIMAAALHESSNVPLIGET----TFGKGTVQTAKEYDDGSTVKLTVAKWLTAD 348 >all2500 [M] COG0793 Periplasmic protease Length = 428 Score = 61.6 bits (148), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 106/452 (23%), Positives = 182/452 (40%), Gaps = 82/452 (18%) Query: 93 TKFRSVMVIVCVLGFLPTLLALSIAGEVRYNPVSDLSHKSYTQAFIAMNERLSTEYPFGD 152 TK R V+ V L T+ S+ R + S K + +R +Y G Sbjct: 4 TKSRLVLGATAVT--LSTIAVTSLGIHSRGQALFKASPKELVDEVWQIVQR---QYVDGT 58 Query: 153 WKKVDWAAKKNKYGPLFRQAEISQDPNLYYKTLRDYLFSFRDGHIQIVNEHLYDGNSVFK 212 + +VDW A + +Y L + Q+ YK++R+ L D + + ++ + V Sbjct: 59 FNQVDWQAVRKEY--LNKSYSNQQEA---YKSIREMLKRLNDPYTRFMDPQEFKNMQVDT 113 Query: 213 NEVGGGVGISTVQLDDGKVLVSLL-IPGSPADQYGIKLGAEIISWDGKDVKKALDETSWS 271 + G+GI+ Q + K LV + I +PA + GI I+ DGK K +D Sbjct: 114 SGELTGIGITISQDEKTKQLVVIAPIEDTPAFKAGILAKDVILKIDGKSTK-GMD----- 167 Query: 272 ENPPATEGHRILNQGRFLARAPIGQNIQVKFR--NQDDP-AIITAALTAYDDQYEXXXXX 328 NQ L R G + + + NQ+ I+ A + + +Y Sbjct: 168 -----------TNQAVNLIRGTAGSQVTLTIQRNNQEKQFKIVRARIEIHPVRYS----- 211 Query: 329 XXXXXXEDPPVEGQILKENYGYVKIRYFLPGATQSDPAKALEDTIKEFQEKQVKGIIIDL 388 + P G++ GY+++ F A+ K +++ IK +++QV G I+DL Sbjct: 212 ------QKPTAVGKV-----GYIRLNQFSANAS-----KEMQEAIKNLEKQQVAGYILDL 255 Query: 389 RDNPGGDDDLVAQMAGHFVNRKRIFEYVSYYNRNTGRFEINRAETRTVQPSRIYFPGKIA 448 R NPGG ++A ++++ I VS +R R R V R + Sbjct: 256 RGNPGGLLFSSVEIARMWMDKGTI---VSTVDRQGER-------EREVANGRALTNKPLV 305 Query: 449 VLINHNTASSGEGLPLILKGMPNVKIIGFTSTNGSFGIVSAPIEVKMPEGYVVQF----- 503 +L++ +AS+ E L L+ I+G T T G G+V + + G V Sbjct: 306 ILVDKGSASASEILSGALQDNKRAVIVG-TQTFGK-GLVQSVRPLDDGSGLAVTIAKYLT 363 Query: 504 PDGRSLNLDYKIQGDSDERGQGGVTPDITVPM 535 P+ R +N + G+ PD+ V + Sbjct: 364 PNDRDIN-------------KHGIDPDVKVEL 382 >BS_yvjB [M] COG0793 Periplasmic protease Length = 480 Score = 59.3 bits (142), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 66/286 (23%), Positives = 124/286 (43%), Gaps = 47/286 (16%) Query: 218 GVGISTVQLDDGKVLVSLLIPGSPADQYGIKLGAEIISWDGKDVKKALDETSWSENPPAT 277 G+G + V ++DGK+++ SPA++ G+K EIIS +G+ + Sbjct: 113 GIG-AEVGMEDGKIIIVSPFKKSPAEKAGLKPNDEIISINGESM---------------- 155 Query: 278 EGHRILNQGRFLARAPIGQNIQVKFRNQDDPAIITAALTAYDDQYEXXXXXXXXXXXEDP 337 + LN R G ++ +K + ++ + + E Sbjct: 156 -AGKDLNHAVLKIRGKKGSSVSMKIQRPGTKKQLSFRIKRAEIPLETVFAS--------- 205 Query: 338 PVEGQILKENYGYVKIRYFLPGATQSDPAKALEDTIKEFQEKQVKGIIIDLRDNPGGDDD 397 E ++ + GY+ I F T D AKAL +E ++K+++G++ID+R NPGG Sbjct: 206 --EKKVQGHSVGYIAISTFSE-HTAEDFAKAL----RELEKKEIEGLVIDVRGNPGGYLQ 258 Query: 398 LVAQMAGHFVNRKRIFEYVSYYNRNTGRFEINRAETRTVQPSRIYFPGKIAVLINHNTAS 457 V ++ HFV + + + ++ N + R+ T+ + Y + V+ + +AS Sbjct: 259 SVEEILKHFVTKDQPYIQIAERNGDKKRY------FSTLTHKKAY---PVNVITDKGSAS 309 Query: 458 SGEGLPLILKGMPNVKIIGFTSTNGSFGIVSAPIEVKMPEGYVVQF 503 + E L LK + ++G T SFG + V M +G ++ Sbjct: 310 ASEILAGALKEAGHYDVVGDT----SFGKGTVQQAVPMGDGSNIKL 351 >CC3435 [M] COG0793 Periplasmic protease Length = 464 Score = 52.8 bits (125), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 39/138 (28%), Positives = 66/138 (47%), Gaps = 15/138 (10%) Query: 344 LKENYGYVKIRYFLPGATQSDPAKALEDTIKEFQEK--QVKGIIIDLRDNPGGDDDLVAQ 401 ++ +YGYV++ F AT AL ++ E + K ++KG+I+DLR+NPGG D Sbjct: 192 MEGDYGYVRLPGFNEKATD-----ALASSVSELKSKNPKMKGLILDLRNNPGGLLDQAVG 246 Query: 402 MAGHFVNRKRIFEYVSYYNRNTGRFEINRAETRTVQPSRIYFPGKIAVLINHNTASSGEG 461 +A F++ + RN R+ + P + VLIN +AS+ E Sbjct: 247 VADVFLDGGEVVSQRGRDPRNIQRYNARGGDMLNGLP--------MVVLINQGSASAAEI 298 Query: 462 LPLILKGMPNVKIIGFTS 479 + L+ +++G TS Sbjct: 299 VAGALQDRKRAEVVGLTS 316 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.140 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 42,677,458 Number of extensions: 1797726 Number of successful extensions: 5544 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 5549 Number of HSP's successfully gapped: 7 Length of query: 561 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 449 Effective length of database: 25,461,023 Effective search space: 11431999327 Effective search space used: 11431999327 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)