BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0342.0024.p (489 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP0886 [V] COG0286 Type I restriction-modification system methyl... 535 e-152 MA2414 [V] COG0286 Type I restriction-modification system methyl... 373 e-103 alr3620 [V] COG0286 Type I restriction-modification system methy... 175 1e-43 alr3475 [V] COG0286 Type I restriction-modification system methy... 173 5e-43 SP0509 [V] COG0286 Type I restriction-modification system methyl... 161 2e-39 >SP0886 [V] COG0286 Type I restriction-modification system methyltransferase subunit Length = 497 Score = 535 bits (1377), Expect = e-152, Method: Compositional matrix adjust. Identities = 268/498 (53%), Positives = 355/498 (71%), Gaps = 13/498 (2%) Query: 1 MLTGEVRNKVDKIWSDIWAGGISNPLTVIEQLTYLMFIRSLDEKELENESFEALSGEKMP 60 M+TGE++NK+D++W +W G +NPLT IEQLTYL+F++ LD EL ES G Sbjct: 1 MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSVELGRESDAEFLGIPYE 60 Query: 61 KIFPENEEGQELRWSKFKT-KDARIIYDIVSTKVFPFIKAMNG-ANTTAFSRYMQDAMFL 118 +FP+++ E RWS FK DA+ +Y +++ ++FPFIK + G + TAFSRYM++A+F Sbjct: 61 GVFPKDK--PEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGDTDDTAFSRYMREAIFQ 118 Query: 119 VPTPQVLQKMIT--------GLDELYEHDIKNLDMQGDLYEYMLGKLASAGQNGQFRTPK 170 + P LQK I+ GLD +++D +++ GD+YEY+L KL++AG+NGQFRTP+ Sbjct: 119 INKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEYLLSKLSTAGKNGQFRTPR 178 Query: 171 HIRDLMVRLIAPTPNDKIVDPACGTAGFLVSSAEYI-REKYEAEMTSEQWEHFSGEMFSG 229 HI D+MV L+ PT D I DPA G+AGFLVS++ Y+ R+K E E ++ HF +MF G Sbjct: 179 HIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKKDEWETNTDNINHFHNQMFHG 238 Query: 230 YDTDRTMLRLSAMNLMLHSISQPHIDYVDSVSRQNNTASAYDIVLANPPFTGTVDAESIN 289 DTD TMLRL AMN+MLH + P I Y+DS+S+ N A Y +VLANPPF G++D S + Sbjct: 239 NDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEADKYTLVLANPPFKGSLDYNSTS 298 Query: 290 ENLRTITDTKKTELLFVALFLRILRKGGRCACIVPDGVLFGSTKAHKSLRKELVENHQLQ 349 +L TKKTELLF++LFLR L+ GGR A IVPDGVLFGS+KAHK +R+E+VENH+L Sbjct: 299 NDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPDGVLFGSSKAHKGIRQEIVENHKLD 358 Query: 350 AVISMPSGVFKPYAGVSTAILLFTKTDAGGTDKVWFYDMKADGYSLDDKRSXXXXXXXXX 409 AVISMPSGVFKPYAGVSTAIL+FTKT GGTDKVWFYDMKADG SLDDKR Sbjct: 359 AVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKVWFYDMKADGLSLDDKRQPISDNDIPD 418 Query: 410 XXXRYHNLEGEADRQPTEPSFLVDKSAIEANDYDLSINRYKEVVYEKVEYDKPEVIMTRL 469 R+H+LE EA+RQ T+ SF V + I+ NDYDLSIN+YKE+ YEKVEY+ EVI+ ++ Sbjct: 419 IIERFHHLEKEAERQRTDQSFFVPVAEIKENDYDLSINKYKEIEYEKVEYEPTEVILKKI 478 Query: 470 DELSLDISSKMEELRGLL 487 ++L +I + + EL LL Sbjct: 479 NDLEKEIQAGLAELEKLL 496 >MA2414 [V] COG0286 Type I restriction-modification system methyltransferase subunit Length = 301 Score = 373 bits (957), Expect = e-103, Method: Compositional matrix adjust. Identities = 0/357 (0%), Positives = 0/357 (0%), Gaps = 22/357 (6%) Query: 154 LGKLASAGQNGQFRTPKHIRDLMVRLIAPTPNDKIVDPACGTAGFLVSSAEYIREKYEAE 213 G + K + + + L E A Sbjct: 64 PGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAV 123 Query: 214 MTSEQW-EHFSGEMFSGYDTDRTMLRLSAMNLMLHSISQPHIDYVDSVSRQ-NNTASAYD 271 + E + + ++ I + D Sbjct: 124 NKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPED 183 Query: 272 IVLANPPFTGTVDAESINENLRTITDTKKTELLFVALFLRILRKGGRCACIVPDGVLFGS 331 + + + + + + F+ R G ++ Sbjct: 184 YITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILVEREGQK 243 Query: 332 TKAHKSLRKELVENHQLQAVISMPSGVFKPYAGVSTAILLFTKTDAGGTDKVWFYDMKAD 391 Sbjct: 244 303 Query: 392 GYSLDDKRSX---------XXXXXXXXXXXRYHNL-----------EGEADRQPTEPSFL 431 Sbjct: 304 363 Query: 432 VDKSAIEANDYDLSINRYKEVVYEKVEYDKPEVIMTRLDELSLDISSKMEELRGLLG 488 Sbjct: 364 420 >alr3620 [V] COG0286 Type I restriction-modification system methyltransferase subunit Length = 359 Score = 175 bits (444), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 0/476 (0%), Positives = 0/476 (0%), Gaps = 45/476 (9%) Query: 20 GGISNPLTVIEQLTYLMFIRSLDEKELENESFEALSGEKMPKIFPENEEGQELRWSKFKT 79 + + + + + + + + Sbjct: 18 QRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVA-------RL--EEALRLRA 68 Query: 80 KDARI----IYDIVSTKVFPFIKAMNGANTTA----FSRYMQDAMFLVPTPQVLQKMITG 131 + + P I A + + + ++ T + Sbjct: 69 GGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMH 128 Query: 132 LDELYEHDIKNLDMQGDLYEYMLGKLASAGQNGQFRTPKHIRDLMVRLIAPTPNDKIVDP 191 + + + + + + + PK I T + Sbjct: 129 RLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPGQ 188 Query: 192 ACGTAGFLVSSAEYIREKYEAEMTSEQWEHFSGEMFSGYDTDRTMLRLSAMNLMLHSISQ 251 A + + T M+L I+ Sbjct: 189 RSIAASGGILLWPQSHFDWVRPGIILYGVS----PLEDRSTGADFGCQPVMSLTSSLIAV 244 Query: 252 PHIDYVDSVSRQNNTASAY---DIVLANPPFTGTVDAESINENLRTITDTKKTELLFVAL 308 + V S V+A + + + + + VA+ Sbjct: 245 REHKAGEPVGYGGTWVSERDTRLGVVAMGYG-DGYPRAAPSGTPVLVNGREVPIVGRVAM 303 Query: 309 FLRILRKGGRCACIVPDGVLFGSTKAHKSLRKELVENHQLQAVISMPSGVFKPYAGVSTA 368 + + G + D V+ Sbjct: 304 DMICVDLGPQAQDKAGDPVILWGE- 362 Query: 369 ILLFTKTDAGGTDKVWFYDMK-ADGYSLDDKRSXXXXXXXXXXXXRYHNLEGEADRQPTE 427 Sbjct: 363 -------- 414 Query: 428 PSFLVDKSAIEANDYDLSINRYKEVVYEKVEYDKPEVIMTRLDELSLDISSKMEEL 483 Sbjct: 415 ---------- 460 >alr3475 [V] COG0286 Type I restriction-modification system methyltransferase subunit Length = 359 Score = 173 bits (439), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 0/454 (0%), Positives = 0/454 (0%), Gaps = 35/454 (7%) Query: 20 GGISNPLTVIEQLTYLMFIRSLDEKELENESFEALSGEKMPKIFPENEEGQELRWSKFKT 79 + + + + + + + + Sbjct: 18 QRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVA-------RL--EEALRLRA 68 Query: 80 KDARI----IYDIVSTKVFPFIKAMNGANTTA----FSRYMQDAMFLVPTPQVLQKMITG 131 + + P I A + + + ++ T + Sbjct: 69 GGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMH 128 Query: 132 LDELYEHDIKNLDMQGDLYEYMLGKLASAGQNGQFRTPKHIRDLMVRLIAPTPNDKIVDP 191 + + + + + + + PK I T + Sbjct: 129 RLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPGQ 188 Query: 192 ACGTAGFLVSSAEYIREKYEAEMTSEQWEHFSGEMFSGYDTDRTMLRLSAMNLMLHSISQ 251 A + + T M+L I+ Sbjct: 189 RSIAASGGILLWPQSHFDWVRPGIILYGVS----PLEDRSTGADFGCQPVMSLTSSLIAV 244 Query: 252 PHIDYVDSVSRQNNTASAY---DIVLANPPFTGTVDAESINENLRTITDTKKTELLFVAL 308 + V S V+A + + + + + VA+ Sbjct: 245 REHKAGEPVGYGGTWVSERDTRLGVVAMGYG-DGYPRAAPSGTPVLVNGREVPIVGRVAM 303 Query: 309 FLRILRKGGRCACIVPDGVLFGSTKAHKSLRKELVENHQLQAVISMPSGVFKPYAGVSTA 368 + + G + D V+ Sbjct: 304 DMICVDLGPQAQDKAGDPVILWGE- 362 Query: 369 ILLFTKTDAGGTDKVWFYDMK-ADGYSLDDKRSXXXXXXXXXXXXRYHNLEGEADRQPTE 427 Sbjct: 363 -------- 414 Query: 428 PSFLVDKSAIEANDYDLSINRYKEVVYEKVEYDK 461 Sbjct: 415 448 >SP0509 [V] COG0286 Type I restriction-modification system methyltransferase subunit Length = 487 Score = 161 bits (407), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 112/390 (28%), Positives = 195/390 (50%), Gaps = 33/390 (8%) Query: 20 GGISNPLTVIEQLTYLMFIRSLDEKELENESFEALSGEKMPKIFPENEEGQELRWSKFK- 78 G++ IEQ+++L+F++ D +E+ E L ++ I PE EL+W + Sbjct: 18 AGVNGDAQRIEQMSWLLFLKIYDSREMVWE----LEEDEYESIIPE-----ELKWRNWAH 68 Query: 79 -TKDARII-----YDIVSTKVFPFIKAMNGANT-----TAFSRYMQDAMFLVPTPQVLQK 127 R++ D V+ K+F +K + + T +DA + +L++ Sbjct: 69 AQNGERVLTGDELLDFVNNKLFKELKELEITSNMPIRKTIVKSAFEDANNYMKNGVLLRQ 128 Query: 128 MITGLDELYEHDIKNLDMQGDLYEYMLGKLASAGQNGQFRTPKHIRDLMVRLIAPTPNDK 187 +I +DE+ + ++ D+YE +L + +AG +G+F TP+ D + ++ P + Sbjct: 129 VINVIDEVDFNSPEDRHSFNDIYEKILKDIQNAGNSGEFYTPRAATDFIAEVLDPKLGES 188 Query: 188 IVDPACGTAGFLVSSAEYIREKYEAEMTSEQWEHFSGEMFSGYDTDRTMLRLSAMNLMLH 247 + D ACGT GFL S+ + + + TSE + ++ +F G + L+ NL LH Sbjct: 189 MADLACGTGGFLTSTLNRLSSQRK---TSEDTKKYNTAVF-GIEKKAFPHLLAVTNLFLH 244 Query: 248 SISQPHIDYVDSVS---RQNNTASAYDIVLANPPFTGTVDAESINENLRTITDTKKTELL 304 I P I + +++ R+ +DI++ NPPF G+ + E+I N + +T L Sbjct: 245 EIDDPKIVHGNTLEKNVREYTDDEKFDIIMMNPPFGGS-ELETIKNNFPAELRSSETADL 303 Query: 305 FVALFLRILRKGGRCACIVPDGVLFGSTKAHKSLRKELVENHQLQAVISMPSGVFKPYAG 364 F+A+ + L++ GR I+PDG LFG L+++LV+ L +I +P VF PY G Sbjct: 304 FMAVIMYRLKENGRVGVILPDGFLFGEG-VKTRLKQKLVDEFNLHTIIRLPHSVFAPYTG 362 Query: 365 VSTAILLFTKTDAGGTDKVWFYDMK-ADGY 393 + T IL F KT T++ WFY + DGY Sbjct: 363 IHTNILFFDKTKK--TEETWFYRLDMPDGY 390 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 35,471,104 Number of extensions: 1450119 Number of successful extensions: 2647 Number of sequences better than 1.0e-05: 34 Number of HSP's gapped: 2577 Number of HSP's successfully gapped: 36 Length of query: 489 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 379 Effective length of database: 25,691,691 Effective search space: 9737150889 Effective search space used: 9737150889 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)