BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0342.0023.p (377 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA2415 [V] COG0732 Restriction endonuclease S subunits 220 3e-57 MA2103 [V] COG0732 Restriction endonuclease S subunits 80 5e-15 MJ1218 [V] COG0732 Restriction endonuclease S subunits 72 1e-12 SP0887 [V] COG0732 Restriction endonuclease S subunits 71 3e-12 MJ0130m [V] COG0732 Restriction endonuclease S subunits 69 1e-11 >MA2415 [V] COG0732 Restriction endonuclease S subunits Length = 391 Score = 220 bits (561), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 127/344 (36%), Positives = 198/344 (57%), Gaps = 13/344 (3%) Query: 43 NRGWYYSDLQLEENKYCDKGDLLYAWSASFGPKIWDGPRAIYHYHIWKIVLSEHV---EK 99 N S+ +E++ +G ++ S G G + I ++ + Sbjct: 49 NAARTLSETGSQESRLLPEGTVMVCCIGSLGKVGIAGRTVASNQQINSVIFDPKIIWPRF 108 Query: 100 GYMYYLLQQSTKAIMNQGRGIAMIHATKGGMEKMLIPLPPLETQKQIANTLEVAAELLAM 159 G+ L +S ++ + +++ +K G ++ IP+PPL QK+IA+ L+ A L A Sbjct: 109 GFYACRLLKSRLEVLAPATTVPIVNKSKFG--QLEIPVPPLPEQKRIADILDRAEALRAK 166 Query: 160 RKQQLAELDNLIKSTFYDMFGNPVTNEKGWE---LKHIGDFSMVKIGPFGSLLHVEDYIE 216 R+ L LD L ++ F DMFG+ V+N GW+ LKH + ++IGPFGSLLH EDY+ Sbjct: 167 RRVALEHLDELTQAIFIDMFGDSVSNPMGWKRYPLKHC--VNHIQIGPFGSLLHKEDYVF 224 Query: 217 DGFPLVNPSHIVGNKIVPDMNLTLSPEKFNELKGYVMEKGDIVVGRRGEIGRCAVV--EN 274 G PL+NP+HI KIVPD+N +++ +K EL+ Y +++GD+++GRRGE+GRCA+V E+ Sbjct: 225 GGIPLINPTHIENGKIVPDVNQSITVQKLAELQLYQLQQGDVIMGRRGEMGRCAIVGSEH 284 Query: 275 EGFLCGTGSMFIRI-ENDFLPMVLQRIISSDPMRSILEHQSVGVTMKNLNAGTISNLVVP 333 G LCGTGS+FIR E+ + M LQ +SS+ MR LE S+G T+ NLN G + L + Sbjct: 285 NGTLCGTGSLFIRPDESKAIAMYLQATLSSESMRKHLEGFSLGATLPNLNRGIVGELAIS 344 Query: 334 MIPLLLQTQFANIVTKIEEQKALVKKAIDETQYLFDSLMSEYFE 377 + P+ LQ +F++ + IE+ K K ++ E LF SL F Sbjct: 345 LPPIELQKEFSHHIESIEKLKTTYKSSLTEIDELFLSLQYRAFR 388 >MA2103 [V] COG0732 Restriction endonuclease S subunits Length = 290 Score = 80.5 bits (197), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 74/297 (24%), Positives = 139/297 (46%), Gaps = 37/297 (12%) Query: 101 YMYYLLQQSTKAIMNQGRGIAMIHATKGGMEKMLIPLPPLETQKQIANTLEVAAELLAMR 160 + Y L + K I ++ + + H + + + IP+PPLETQ++I + L+ A E +R Sbjct: 4 FAYRSLVKILKDIEDRTAFVTVKHLSAKQLNTIKIPVPPLETQQKIVSILKKAEETKKLR 63 Query: 161 KQQLAELDNLIKSTFYDMFGNPVTNEKGWELKHIGDFSMVKIG------------PFGSL 208 Q L++S F +MFG+PV N K W+ + D S + G F Sbjct: 64 AQADELTQKLLQSVFLEMFGDPVVNPKNWKEIKLKDVSEIVSGVTKGRKLAGKPTVFVPY 123 Query: 209 LHVEDYIEDGFPLVNPSHIVGNKIVPDMNLTLSPEKFNELKGYVMEKGDIVVGRRGE--- 265 L V + ++DG+ ++ + I +++P ++++ Y ++ GDI++ G+ Sbjct: 124 LRVAN-VQDGY--LDLTEIKEIEVLP-----------SDVEKYALQGGDILLTEGGDPDK 169 Query: 266 IGRCAVVENEGFLCGTGSMFIRIEND---FLPMVLQRIISSDPMRSIL---EHQSVGVTM 319 +GR AV + C + R+ + +P L +I S + Q+ G+ Sbjct: 170 LGRGAVWNRQIPTCIHQNHIFRVRVNRECLVPEYLSMLIGSTYGKMYFLKSAKQTTGIA- 228 Query: 320 KNLNAGTISNLVVPMIPLLLQTQFANIVTKIEEQKALVKKAIDETQYLFDSLMSEYF 376 ++N+ + N + L LQ +FA +V +IE+ +++ + LFDSLM + F Sbjct: 229 -SINSTQLKNFPALIASLDLQLRFAEMVHQIEKTTVSQQQSSFKINNLFDSLMQKAF 284 >MJ1218 [V] COG0732 Restriction endonuclease S subunits Length = 425 Score = 72.4 bits (176), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 68/270 (25%), Positives = 116/270 (42%), Gaps = 14/270 (5%) Query: 4 WEKVKLGDVVTLLNGR--AYKQDELLDEGATPVLRVGNFFSNRGWYYSDLQLEENKYCDK 61 WE V++GD + + G+ A DE L EG P L + + +E ++ Sbjct: 25 WEVVRIGDFIKYIKGKKPAVMVDEEL-EGYYPYLSTEYLRDGIASKFVKITNKEI-IVNE 82 Query: 62 GDLLYAWSASFGPKIWDGPRAIYHYHIWKIVLSEHV-EKGYMYYLLQQSTKAIMNQGRGI 120 D+L W S +I+ G + I + K+ + + Y++Y L+ + +Q +G Sbjct: 83 NDILLLWDGSNAGEIFLGKKGILSSTMVKLEQKNKIMDDLYLFYSLKLKESFLKSQTKGT 142 Query: 121 AMIHATKGGMEKMLIPLPPLETQKQIANTLEVAAELLAMRKQQLAELDNLIKSTFYDMFG 180 + H K E + IPLPPLE QKQIA L L+ +Q+ L+ K +F Sbjct: 143 GIPHVDKKIFENIKIPLPPLEEQKQIAKILSDFDNLIGTINKQIEVLNKAKKGMMKKLFT 202 Query: 181 NPVTNEKGWELKHIG----DFSMVKIGPF-----GSLLHVEDYIEDGFPLVNPSHIVGNK 231 V K ++ IG D+ +VK+ G ++ E+G + ++ K Sbjct: 203 KGVFEHKSFKKSEIGEIPEDWEVVKLKEVVDIQSGKYFKYSEFCENGVKCLKIDNVGFGK 262 Query: 232 IVPDMNLTLSPEKFNELKGYVMEKGDIVVG 261 I + L + N+ V++ GDIV+ Sbjct: 263 IFWETVSFLPEDYLNKYPQLVLKSGDIVLA 292 >SP0887 [V] COG0732 Restriction endonuclease S subunits Length = 373 Score = 71.2 bits (173), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 93/397 (23%), Positives = 169/397 (42%), Gaps = 51/397 (12%) Query: 5 EKVKLGDVVTLLNGRAYKQDELLDEGAT---PVLRVGNFFSNRGWYYSD-LQLEENKYCD 60 +KVKLG+V++L G K+ +L E T +++ + +N +++ L + E Sbjct: 2 KKVKLGEVLSLKKG---KKATVLAEQTTLSQRYIQIDDLRNNNNLKFTESLNMTE---AL 55 Query: 61 KGDLLYAWSASFGPKIWDGPRAIYHYHIWKIVLSEHVEK----GYMYYLLQQSTKAIMNQ 116 D+L AW + + G I + +E ++ Y+ L+ ++ + + Sbjct: 56 PDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLGVFLESKSQYLRDH 115 Query: 117 GRGIAMIHATKGGMEKMLIPLPPLETQKQIANTLEVAAELLAMRKQQLAELDNLIKSTFY 176 G + H K + + + L +E Q+ I L L+ RK QL EL+ L+KS F Sbjct: 116 STGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRKFQLDELNLLVKSRFN 175 Query: 177 DMFGNPVTNEKGWELKHIGDFSMVKIGPFGSLLHVEDYIEDGFPLVNPSHIVGNKIVPDM 236 +MFG E L I D K P L E+Y +N ++ N D Sbjct: 176 EMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYC----LFLNTKNVTKNGFSFDT 231 Query: 237 NLTLSPEKFNELKGYVMEKGDIVVGRRGEIGRCA----VVENEGFLCGTGSMFIRIENDF 292 ++ K L+ +E+ DIV+ RG +G A +++ + +G + +R + Sbjct: 232 KQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHLRINSGMVILRPKT-- 289 Query: 293 LPMVLQRIISSDPMRSILEHQSVGVTMKNLNAGTISNLVVPMIPLL-------------L 339 P + Q+ I + V N + IS P +P+ L Sbjct: 290 -PNLNQKFI-------------IHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLAL 335 Query: 340 QTQFANIVTKIEEQKALVKKAIDETQYLFDSLMSEYF 376 Q +FA+ V ++++ + ++K+++E + L SLM EYF Sbjct: 336 QNEFADFVVQVDKSQLAIQKSLEELETLKKSLMQEYF 372 >MJ0130m [V] COG0732 Restriction endonuclease S subunits Length = 425 Score = 69.3 bits (168), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 86/386 (22%), Positives = 159/386 (41%), Gaps = 49/386 (12%) Query: 4 WEKVKLGDVVTLLNGRAYKQ---DELLDEGATPVLRVGNFF-SNRGWYYSDLQLEENKYC 59 WE V+L DV + + +E G P +++ + SN+ + +++ E Sbjct: 20 WEIVELKDVCKKIKAGGTPKTSVEEYYKNGTIPFVKIEDITNSNKYLTNTKIKITEEGLN 79 Query: 60 D-------KGDLLYAWSASFGPKIWDGPRAIYHYHIWKIVLSEHV-EKGYMYYLLQQSTK 111 + K +L+A S G + + I I+ +++ E ++YY+L ++ Sbjct: 80 NSNAWIVPKNSVLFAMYGSIGETAINKIEVATNQAILGIIPKDNILESEFLYYILAKNKN 139 Query: 112 AIMNQGRGIAMIHATKGGMEKMLIPLPPLETQKQIANTLEVAAELLAMRKQQLAELDNLI 171 G + ++ IPLPPLE QKQIA L E + + ++ + +L+ + Sbjct: 140 YYSKLGMQTTQKNLNAQIVKSFKIPLPPLEEQKQIAKILTKIDEGIEIIEKSINKLERIK 199 Query: 172 KSTFYDMFGNPVTNEK-----------GWELKHIGDFSMVKIGPFGSLLHVEDYIEDG-- 218 K + + + + + WE+ I D VK G S E Y E+G Sbjct: 200 KGLMHKLLTKGIGHSRFKKSEIGEIPEDWEVFEIKDIFEVKTGTTPSTKKSE-YWENGEI 258 Query: 219 ---FPL----VNPSHIVGNKIVPDMNLTLSPEKFNELKGYVMEKGDIVVGRRGEIGRCAV 271 PL +N +G+ + L N ++ KG I++ R +G AV Sbjct: 259 NWITPLDLSRLNEKIYIGSSERKVTKIALEKCNLN-----LIPKGSIIISTRAPVGYVAV 313 Query: 272 VENEG-FLCGTGSMFIR----IENDFLPMVLQRIISSDPMRSILEHQSVGVTMKNLNAGT 326 + E F G +F + + +F L+ +++LE+ S G T K L+ Sbjct: 314 LTVESTFNQGCKGLFQKNNDSVNTEFYAYYLKF------KKNLLENLSGGSTFKELSKSM 367 Query: 327 ISNLVVPMIPLLLQTQFANIVTKIEE 352 + N +P+ PL Q Q A I++ +++ Sbjct: 368 LENFKIPLPPLEEQKQIAKILSSVDK 393 Score = 56.2 bits (134), Expect = 7e-08, Method: Compositional matrix adjust. Identities = 49/191 (25%), Positives = 89/191 (46%), Gaps = 14/191 (7%) Query: 189 WELKHIGDFSMVKIGPFGS-LLHVEDYIEDG-FPLVNPSHIV-GNKIVPDMNLTLSPEKF 245 WE+ + D KI G+ VE+Y ++G P V I NK + + + ++ E Sbjct: 20 WEIVELKDVCK-KIKAGGTPKTSVEEYYKNGTIPFVKIEDITNSNKYLTNTKIKITEEGL 78 Query: 246 NELKGYVMEKGDIVVGRRGEIGRCAV----VENEGFLCGTGSMFIRIENDFLPMVLQRII 301 N +++ K ++ G IG A+ V + G +E++FL +L + Sbjct: 79 NNSNAWIVPKNSVLFAMYGSIGETAINKIEVATNQAILGIIPKDNILESEFLYYILAK-- 136 Query: 302 SSDPMRSILEHQSVGVTMKNLNAGTISNLVVPMIPLLLQTQFANIVTKIEEQKALVKKAI 361 ++ + T KNLNA + + +P+ PL Q Q A I+TKI+E +++K+I Sbjct: 137 ----NKNYYSKLGMQTTQKNLNAQIVKSFKIPLPPLEEQKQIAKILTKIDEGIEIIEKSI 192 Query: 362 DETQYLFDSLM 372 ++ + + LM Sbjct: 193 NKLERIKKGLM 203 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.138 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,673,508 Number of extensions: 1227836 Number of successful extensions: 2777 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 2768 Number of HSP's successfully gapped: 10 Length of query: 377 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 269 Effective length of database: 25,922,359 Effective search space: 6973114571 Effective search space used: 6973114571 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)