BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0342.0021.p (251 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA2133 [S] COG3177 Uncharacterized conserved protein 118 7e-27 NMA2133 [S] COG3117 Uncharacterized protein conserved in bacteria 118 7e-27 mlr2757 [S] COG3177 Uncharacterized conserved protein 83 5e-16 NMA2029 [S] COG3177 Uncharacterized conserved protein 70 3e-12 alr0502 [S] COG3177 Uncharacterized conserved protein 60 2e-09 >MA2133 [S] COG3177 Uncharacterized conserved protein Length = 326 Score = 118 bits (296), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 70/210 (33%), Positives = 112/210 (53%), Gaps = 3/210 (1%) Query: 35 FELEYTHNSTAIEGNXXXXXXXXXXXXXXXSVGGKKLREIYEVINHNKAYQHVKACIEQG 94 L +T++S AIEGN ++GGK LRE E N+ KA+ + ++ Sbjct: 58 MRLVHTYHSNAIEGNTLTLQETKLVLEEELTIGGKSLREHLEATNNAKAFDRMGELAKKK 117 Query: 95 LLLDENIIKDIHAILMENIMV-GGVYRNVEVYISGAAHTPPIPNEMYRQVQNFYADLADK 153 +D II++IH I+ I+ G YR + I+GA PP +++ + + +A+ Sbjct: 118 RAIDHVIIQEIHEIVTRGILEDAGRYRTRNIRITGAVKAPPDWSKIVKLMDELIQKIAES 177 Query: 154 KPVNIIELAAWTHAEFVRIHPFADGNGRTSRLIMNYQLLANGFLPISIAKESRLDYFNAL 213 K + IE A++ H FV IHPF+DGNGR +RL+ N L+A + P+ + KE Y+ +L Sbjct: 178 KK-HPIETASFLHHRFVEIHPFSDGNGRAARLLTNLYLIARDYPPVVLKKEDWGKYYKSL 236 Query: 214 EAYAVHRDLRPFADMMASLEEQQLDRYLGM 243 A A +L PFA+ +A ++ L YL + Sbjct: 237 RA-ADSGNLEPFANFIAKAVDENLTLYLSI 265 >NMA2133 [S] COG3117 Uncharacterized protein conserved in bacteria Length = 326 Score = 118 bits (296), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 70/210 (33%), Positives = 112/210 (53%), Gaps = 3/210 (1%) Query: 35 FELEYTHNSTAIEGNXXXXXXXXXXXXXXXSVGGKKLREIYEVINHNKAYQHVKACIEQG 94 L +T++S AIEGN ++GGK LRE E N+ KA+ + ++ Sbjct: 58 MRLVHTYHSNAIEGNTLTLQETKLVLEEELTIGGKSLREHLEATNNAKAFDRMGELAKKK 117 Query: 95 LLLDENIIKDIHAILMENIMV-GGVYRNVEVYISGAAHTPPIPNEMYRQVQNFYADLADK 153 +D II++IH I+ I+ G YR + I+GA PP +++ + + +A+ Sbjct: 118 RAIDHVIIQEIHEIVTRGILEDAGRYRTRNIRITGAVKAPPDWSKIVKLMDELIQKIAES 177 Query: 154 KPVNIIELAAWTHAEFVRIHPFADGNGRTSRLIMNYQLLANGFLPISIAKESRLDYFNAL 213 K + IE A++ H FV IHPF+DGNGR +RL+ N L+A + P+ + KE Y+ +L Sbjct: 178 KK-HPIETASFLHHRFVEIHPFSDGNGRAARLLTNLYLIARDYPPVVLKKEDWGKYYKSL 236 Query: 214 EAYAVHRDLRPFADMMASLEEQQLDRYLGM 243 A A +L PFA+ +A ++ L YL + Sbjct: 237 RA-ADSGNLEPFANFIAKAVDENLTLYLSI 265 >mlr2757 [S] COG3177 Uncharacterized conserved protein Length = 263 Score = 82.8 bits (203), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 62/211 (29%), Positives = 97/211 (45%), Gaps = 9/211 (4%) Query: 37 LEYTHNSTAIEGNXXXXXXXXXXXXXXXSVGGKKLREIYEVINHNKAYQHVKACIEQGLL 96 ++ T+ S AIEGN ++ GK L++ E ++H +A V Q Sbjct: 35 IDITYTSNAIEGNTLTAGETALVLEKGITISGKPLKDHLEAVDHARALNWVLEIASQSST 94 Query: 97 -LDENIIKDIHAILMENIM--VGGVYRNVEVYI---SGAAHTPPIPNEMYRQVQNFYADL 150 + E I+++H +++ N + G Y + Y+ +G PP P ++ ++ F L Sbjct: 95 PIVEADIRNLHHLVVANTKPEIAGRYADSARYVNTDAGVYDFPP-PMDVPALMERFCQWL 153 Query: 151 ADKKPVNIIELAAWTHAEFVRIHPFADGNGRTSRLIMNYQLLANGFLPISIAKESRLDYF 210 + N + A H V IHPF DGNGRT+RL+MN L G+ P++I E R Y Sbjct: 154 GTAR--NTPQTAFEAHRRLVDIHPFNDGNGRTARLLMNLVLARAGYPPVAIRPEDRPAYI 211 Query: 211 NALEAYAVHRDLRPFADMMASLEEQQLDRYL 241 ALE F ++ +Q LD YL Sbjct: 212 AALETAQRGGGHAAFDRLLFDRLDQTLDMYL 242 >NMA2029 [S] COG3177 Uncharacterized conserved protein Length = 139 Score = 70.1 bits (170), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 0/213 (0%), Positives = 0/213 (0%), Gaps = 5/213 (2%) Query: 32 VQAFELEYTHNSTAIEGNXXXXXXXXXXXXXXXSVGGKKLREIYEVINHNKAYQHVKACI 91 +L + + + S K I + A K Sbjct: 65 KLLGKLYNIRTTVERKNSNDVVVYNRSKTPTRGSEWAKIYVSISNIAALLTALTAFKVGR 124 Query: 92 EQGLLLDE-NIIKDIHAILMENIM---VGGVYRNVEVYISGAAHTPPIPNEMYRQVQNFY 147 + Sbjct: 125 HDLIRAPGA- 183 Query: 148 ADLADKKPVNIIELAAWTHAEFVRIHPFADGNGRTSRLIMNYQLLANGFLPISIAKESRL 207 Sbjct: 184 243 Query: 208 DYFNALEAYAVHRDLRPFADMMASLEEQQLDRY 240 Sbjct: 244 276 >alr0502 [S] COG3177 Uncharacterized conserved protein Length = 359 Score = 60.5 bits (145), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 4/55 (7%), Positives = 11/55 (20%) Query: 159 IELAAWTHAEFVRIHPFADGNGRTSRLIMNYQLLANGFLPISIAKESRLDYFNAL 213 A + L + + L + ++R + Sbjct: 38 YGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISA 92 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.135 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,644,672 Number of extensions: 706569 Number of successful extensions: 2561 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 2552 Number of HSP's successfully gapped: 16 Length of query: 251 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 147 Effective length of database: 26,383,695 Effective search space: 3878403165 Effective search space used: 3878403165 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)