BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0342.0019.p (407 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value TM0342 [GEPR] COG0477 Permeases of the major facilitator superfa... 72 1e-12 PAB0724 [GEPR] COG0477 Permeases of the major facilitator superf... 61 3e-09 BH0293 [GEPR] COG0477 Permeases of the major facilitator superfa... 57 7e-08 PAB0754 [GEPR] COG0477 Permeases of the major facilitator superf... 56 8e-08 VNG2231C [GEPR] COG0477 Permeases of the major facilitator super... 54 7e-07 >TM0342 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 413 Score = 72.4 bits (176), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 71/356 (19%), Positives = 150/356 (42%), Gaps = 14/356 (3%) Query: 28 ALALAYWVLQTTGSPSLTGLVLFFSMAPAIITGLFSGGIIDFFNRKKLMVNIDIIRGLVV 87 A+A+ +VL T S S L+ M P I+ +G + D +RK LMV++D +RG ++ Sbjct: 29 AVAIPLFVLDLTHSGSAVALMTVAYMVPRILFTPIAGIVGDRGDRKFLMVSMDFLRGFLI 88 Query: 88 SIVAISYYAGALNMPIIITAQVILSICSAFFDPTVQAVIPQIVEKGELISANARSQFXXX 147 +A+ + ++ +P++ +Q+ ++I FD A+ P IV + L+ AN+ Sbjct: 89 CFLALLAFRNSITLPVLYVSQIAMAIMDNLFDIPTGAMFPDIVPQDFLMRANSIMGMVRI 148 Query: 148 XXXXXXXXXXXICAAWLGYGXXXXXXXXXXXXXXXXXXXXXXKPVTYTEPSK-KKIRENI 206 + + G +P+K +I E++ Sbjct: 149 FPQILGPALGGVIYGFYGIAIVFLVNGVSFVLSAISEIFIVYSRKVEKKPAKFSQILEDL 208 Query: 207 QEGYKYIFKNRSIILIVTVVAILHFFVGSV-QVIMPVFALTLKGNGAQNLGIIESFYGTG 265 +EG+ +I+ + I I+ L+ + + V+ P + AQ G +E+ + G Sbjct: 209 KEGFLFIWNHELIRTILIFALFLNLLISPLFMVVYPYTIREVMKFSAQEFGFLETSFTLG 268 Query: 266 VIVTGFILSLTSINGQEKKYMYGGIGF------IGLVYMVFGILSNSGVDEXXXXXXFI- 318 ++ F+++ + KK M+ + + +V++ F ++ + ++ F Sbjct: 269 ALLGNFLIA----SYLSKKNMWKAMKISIFLFELPIVFLGFLVMPDFSLNRLVLFSLFFI 324 Query: 319 -FFAMSSAVVVISTCYRAILQQMVENEMSGRVFGIIGAVGNFTYPVAMLLFGIMLE 373 F M +I+ Q+M + + R F + V N P+ +L+G +++ Sbjct: 325 TFLIMGFLNPIINVPLDTAFQKMTPSHIRARAFSFLILVANGVTPLGSVLYGYLVD 380 >PAB0724 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 418 Score = 61.2 bits (147), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 39/113 (34%), Positives = 65/113 (57%), Gaps = 5/113 (4%) Query: 29 LALAYWVLQTTGSPSLTGLVLFFSMAPAIITGLFSGGIIDFFNRKKLMVNIDIIRG-LVV 87 +AL +VL T S + L + + P+IIT +G D ++RKK+MV D+IRG L+ Sbjct: 24 VALPLYVLDVTHSAKIMTLFVLADLIPSIITTPIAGVFGDRYDRKKIMVGFDLIRGVLLF 83 Query: 88 SIVAISYYAGALNMPIIITAQVILSICSAFFDPTVQAVIPQIVEKGELISANA 140 ++A ++ L + ++ QVI++I +AFF A+ P +V+ EL AN+ Sbjct: 84 LVIAFNF----LTIRCLLVVQVIMAIMNAFFSAGTGAMYPDLVKPEELERANS 132 >BH0293 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 418 Score = 56.6 bits (135), Expect = 7e-08, Method: Compositional matrix adjust. Identities = 81/390 (20%), Positives = 164/390 (42%), Gaps = 26/390 (6%) Query: 27 YALALAYWVLQTTGSPSLTGLVLFFSMAPAIITGLFSGGIIDFFNRKKLMVNIDIIRGLV 86 Y L +++V+ G + G+VL P ++ + G + D F R +M I R ++ Sbjct: 43 YFLTKSWYVVDELGLKTFLGVVLMAMTVPRVLLMMIGGVLADRFRRSTIMFCSLITRSVL 102 Query: 87 VSIVAISYYAGALNMPIIITAQVILSICSAFFDPTVQAVIPQIVEKGELISANARSQFXX 146 + + I + G +N+ +I + I AFF P A++P IV+K L AN+ Q Sbjct: 103 LFGMFILQFLGVINIWTLIGFAFCIGILDAFFWPANYAILPSIVKKDHLTRANSFLQ--- 159 Query: 147 XXXXXXXXXXXXICAAWL----GYGXXXXXXXXXXXXXXXXXXXXX---XKPVTYTEPSK 199 + A WL Y KP T PS Sbjct: 160 -STNHLTLIIGPMIAGWLITITAYEWIFVTIMCLLIIGAILIFRVKEPFDKPETKRLPSP 218 Query: 200 KKIRENIQEGYKYIFKNRSIILIVTVVAILHFFV-GSVQVIMP-VFALTLKGNGAQNLGI 257 K ++ EGY Y+ + ++ ++ + ++FF+ G + +P + LKG+ + L Sbjct: 219 LK---DLTEGYSYLRHSPVLLSLMWMSVFVNFFITGPGTMAIPLIVEERLKGDPLE-LSY 274 Query: 258 IESFYGTGVIVTGFILSLTSINGQEKKYMYGGIGFIGLVYMVFGILSNSGVDEXXXXXXF 317 +ES G+I+ ++ + ++ + + + +G+ + G + + F Sbjct: 275 LESTIAGGLILGAIMIGIINLRSKRGLLIIIRLTVMGVAIALLGQVVS------LWQAIF 328 Query: 318 IFFAMSSAVVVISTCYRAILQQMVENEMSGRVFGIIGAVGNFTYPVAMLLFGIMLEW-FK 376 + + + + RA++Q+ + + GRV G++ + P++ + + L Sbjct: 329 VLAILGMGISIGDIPARAVVQEKTDFDKIGRVMGMMSTASSGLIPLSYGMTSVALSLNLS 388 Query: 377 YGLLLMFCGSIVIVLG-LALFKLNR-KSIE 404 +L++CGS VI+ L+K ++ +S+E Sbjct: 389 ISSILLYCGSFVILFSFFVLWKFDKLRSLE 418 >PAB0754 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 398 Score = 56.2 bits (134), Expect = 8e-08, Method: Compositional matrix adjust. Identities = 38/115 (33%), Positives = 60/115 (52%), Gaps = 3/115 (2%) Query: 29 LALAYWVLQTTGSPSLTGLVLFFSMAPAIITGLFSGGIIDFFNRKKLMVNIDIIRGLVVS 88 +A+ +VL TGS ++ L + + P I+ +G I D +NRK LMV D+IRG+++ Sbjct: 27 VAIPLYVLDKTGSGTMMSLFVMAELIPRILVNPIAGVIGDRYNRKYLMVGFDLIRGVLLF 86 Query: 89 IVAISYYAGALNMPIIITAQVILSICSAFFDPTVQAVIPQIVEKGELISANARSQ 143 V + +I QVI+SI +FF + P +V K L+ AN+ Q Sbjct: 87 FVLFFNLLELEQLLVI---QVIMSIFGSFFAAGTSGMFPDLVPKDRLMQANSILQ 138 >VNG2231C [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 447 Score = 53.5 bits (127), Expect = 7e-07, Method: Compositional matrix adjust. Identities = 27/117 (23%), Positives = 65/117 (55%), Gaps = 1/117 (0%) Query: 24 DKFYALALAYWVLQTTGSPSLTGLVLFFSMAPAIITGLFSGGIIDFFNRKKLMVNIDIIR 83 D Y +A + V + T P +G+ F +M P+ + LF G ++D + +++++ + Sbjct: 24 DSLYYVAAMWLVWELTHDPFYSGVAGFLTMGPSALQFLF-GPLVDRWPLRRVLIGTQAFQ 82 Query: 84 GLVVSIVAISYYAGALNMPIIITAQVILSICSAFFDPTVQAVIPQIVEKGELISANA 140 ++V + ++ Y G L++ +++ +L++ + P A +P+IV++ +L+ AN+ Sbjct: 83 AVLVLAIPLAAYTGHLSVWVVLAVMPLLALANQLVYPAQSAALPRIVDQADLVDANS 139 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.143 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,885,654 Number of extensions: 898057 Number of successful extensions: 2877 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 2878 Number of HSP's successfully gapped: 12 Length of query: 407 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 298 Effective length of database: 25,807,025 Effective search space: 7690493450 Effective search space used: 7690493450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)