BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0035.p (713 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr4662 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase,... 124 7e-28 MA3497 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase, ... 123 1e-27 SMc02580 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase... 121 4e-27 MA4203 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase, ... 118 3e-26 MA3498 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase, ... 117 5e-26 >mlr4662 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase, beta subunit Length = 680 Score = 124 bits (310), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 103/354 (29%), Positives = 175/354 (49%), Gaps = 35/354 (9%) Query: 4 KQIRIGIDVGGTFTDAVAIDNET---------FEVLSKVKMPTTHHDERGVASGIVQIIQ 54 K + +GID GGT+TDAV + +ET +VL+K K TT HD +A GI + Sbjct: 8 KPLFLGIDTGGTYTDAV-LWSETEGPQHGPNKGKVLAKAKALTTRHD---LAVGISGAVD 63 Query: 55 RIMSENGIQPEDIKFIAHGTTQATNALLEGDVARVGILGMGTGLDARSARSETNVA--NI 112 ++ ++G P IK ++ TT ATNAL+EG RV ++ +G SE ++A + Sbjct: 64 AVLEKSGTDPAAIKLVSMSTTLATNALVEGQGGRVALVMIGF--------SEVDLARDGL 115 Query: 113 ELAAGK----YLTTYHKFI-DSKELTREAIDDAIEQLLAEGAEVIVASEAYSVDDPANEL 167 + A G + H ++ L ++ A+ +L A + V + ++ +PA+E+ Sbjct: 116 KTALGTDPVVFCPGGHDVHGNAARLDLSGLEAALPELGASVSGFAVCAY-FATRNPAHEI 174 Query: 168 RVIEAARSR-GVYATGGHEISQLYGLKTRTRTAVVNASLIPKMMETANMTEQAVKEAGIT 226 E R R G+ T HE+S G R T ++NA LI + TE ++ I Sbjct: 175 AARELIRDRTGLPVTASHELSAKLGGPRRALTTLLNARLISMIDRLVAATEGFLQARNIA 234 Query: 227 SQLMIMRCDGGVMSIDEVRKRPILTMXXXXXXXXXXXXMYEKISDGIFFEVGGTSVDISV 286 + LM++R DG ++S R+RPI T+ + + + ++GGT+ D++V Sbjct: 235 APLMVVRGDGALVSAAFARQRPIETILSGPAASLVGARHMTGLENAVVSDIGGTTTDVAV 294 Query: 287 IKNGKVMI--ENAQVGGHKTYLRSLDVRTLAVAGGSMIQIGAGKITD---VGPR 335 + G+ + E A VGG +T + ++ +RT + G S + + G + +GPR Sbjct: 295 LDQGRPRLDPEGATVGGFRTMVEAVAMRTFGLGGDSEVALEDGALNPKILLGPR 348 >MA3497 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase, beta subunit Length = 683 Score = 123 bits (308), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 104/338 (30%), Positives = 162/338 (47%), Gaps = 32/338 (9%) Query: 8 IGIDVGGTFTDAVAIDNETFEVLSKVKMPTTHHDERGVASGIVQIIQRIMSENGIQPEDI 67 +GID GGT+TD++ I N +V K TT+ D + GI I +G+ PE + Sbjct: 5 LGIDAGGTYTDSILIRNSDGKVTCSNKALTTYPD---LLEGITNSI------DGLDPEKL 55 Query: 68 KFIAH---GTTQATNALLEGDVARVGILGMGTGLDARSARSETNVANIELAAGKYLTTYH 124 K + TT ATN +LE VG++ +G +++ VAN + G + Sbjct: 56 KLVTMVSVSTTLATNTILEKTGYPVGLILVGD----YEIPADSEVANCIMVRGGH----- 106 Query: 125 KFIDSKELTREAID-DAIEQL---LAEGAEVIVASEAYSVDDPANELRVIEAARS-RGVY 179 DS+ E++D A+E L + S +SV +P +ELRV + G+ Sbjct: 107 ---DSRGEEVESLDLSAVENFVLRLKDRVSAFAVSSYFSVRNPEHELRVKALIQELTGLP 163 Query: 180 ATGGHEISQLYGLKTRTRTAVVNASLIPKMMETANMTEQAVKEAGITSQLMIMRCDGGVM 239 GHE++Q G R TA +NA L+P ++ G+ S++ ++RCDG V+ Sbjct: 164 VVCGHELAQSLGAYERGVTAYLNAQLLPVAEGFLKTVVNEIERRGLESRIAMLRCDGSVV 223 Query: 240 SIDEVRKRPILTMXXXXXXXXXXXXMYEKISDGIFFEVGGTSVDISVIKNG-KVMIEN-A 297 S+ E K+PI ++ +VGGTS D+S+I G + EN A Sbjct: 224 SMQEAMKKPIESVFSGPAASLLGASYLSGQETCTVIDVGGTSTDVSLIHKGLPYLSENGA 283 Query: 298 QVGGHKTYLRSLDVRTLAVAGGSMIQIGAGKITDVGPR 335 VGG +T +R+L + T A+ G S I + G T++GPR Sbjct: 284 VVGGWQTKVRALRMETSAIGGDSHIWV-QGSRTNIGPR 320 >SMc02580 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase, beta subunit Length = 668 Score = 121 bits (304), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 98/342 (28%), Positives = 170/342 (49%), Gaps = 18/342 (5%) Query: 3 NKQIRIGIDVGGTFTDAVAIDNETFEVLSKVKMPTTHHDERGVASGIVQIIQRIMSENGI 62 + +GID GGT+TDAV + +E V++K K TT HD +A G+ ++ ++++ + Sbjct: 2 TPHLLLGIDTGGTYTDAV-LFSEATGVVAKAKALTTRHD---LAEGVSGAVETVLAKARV 57 Query: 63 QPEDIKFIAHGTTQATNALLEGDVARVGILGMGTGLD--ARSARSETNVANIELAAGKYL 120 I ++ TT ATNAL+EG R G++ +G G + R E ++ L +L Sbjct: 58 PVSAISLVSLSTTLATNALVEGQGGRAGLIMIGFGPEDLKRDGLQEALGSDPVL----FL 113 Query: 121 TTYHKFIDSK-ELTREAIDDAIEQLLAEGAEVIVASEAYSVDDPANELRVIEAARS-RGV 178 H + L A+D+ + L ++ + +A ++V +P +E RV + R + Sbjct: 114 PGGHNVHGGETPLDMSALDETLPDLSSQVSSFAIAGY-FAVRNPDHEKRVRDRIREVSHL 172 Query: 179 YATGGHEISQLYGLKTRTRTAVVNASLIPKMMETANMTEQAVKEAGITSQLMIMRCDGGV 238 T HE+S G R T ++NA L+ + E +K GI +M++R DG + Sbjct: 173 PVTCSHELSSKLGGPRRALTTLLNARLVSMIDRLIGSCEDFLKARGIDVPMMVVRGDGAL 232 Query: 239 MSIDEVRKRPILTMXXXXXXXXXXXXMYEKISDGIFFEVGGTSVDISVIKNGKVMI--EN 296 +S E R RPI T+ + + I ++GGT+ D++V++ G+ + E Sbjct: 233 ISAAEARLRPIETILSGPAASLVGARYLTGLDNAIVSDIGGTTTDVAVLEKGRPRLDAEG 292 Query: 297 AQVGGHKTYLRSLDVRTLAVAGGSMIQI---GAGKITDVGPR 335 A VGG +T + ++ +RT + G S ++I G D+GPR Sbjct: 293 AVVGGFRTMVEAVAMRTYGLGGDSEVKINDRGLKARLDLGPR 334 >MA4203 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase, beta subunit Length = 642 Score = 118 bits (296), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 94/321 (29%), Positives = 156/321 (48%), Gaps = 23/321 (7%) Query: 8 IGIDVGGTFTDAVAIDNETFEVLSKVKMPTTHHDERGVASGIVQIIQRIMSENGIQPE-- 65 +GID GGT+TDAV + + E++ K TT+ D SGI +I +G+ PE Sbjct: 5 LGIDAGGTYTDAVLVKDSDGEIVDLNKALTTYPDP---LSGIKNVI------DGLNPEYL 55 Query: 66 -DIKFIAHGTTQATNALLEGDVARVGILGMGTGLDARSARSETNVANIELAAGKYLTTYH 124 ++K ++ TT +TN +LEG V ++ +G E ++ A+G + H Sbjct: 56 ENVKLVSVSTTLSTNTILEGTGFPVALILIGD----HPLEKELPTGHVLFASGGH---NH 108 Query: 125 KFIDSKELTREAIDDAIEQLLAEGAEVIVASEAYSVDDPANELRVIEA-ARSRGVYATGG 183 + L E I++ +++ + S +S +P +EL+ ++ + G+ A G Sbjct: 109 NGEEVSPLDLEVIEEFALRVM-DKVSAFAVSSYFSTRNPEHELKARDSILKLTGLPAVCG 167 Query: 184 HEISQLYGLKTRTRTAVVNASLIPKMMETANMTEQAVKEAGITSQLMIMRCDGGVMSIDE 243 HE+SQ G R TA +NA LIP + +++ GI +QL++++CDG V+ I + Sbjct: 168 HELSQELGAYERAVTAFLNAQLIPITRQFVQSIIADIRKRGINAQLLMLKCDGSVVGIKD 227 Query: 244 VRKRPILTMXXXXXXXXXXXXMYEKISDGIFFEVGGTSVDISVIKNG--KVMIENAQVGG 301 ++PI T+ + +VGGTS DIS I G + E A VGG Sbjct: 228 ALQKPIETIFSGPAASLVGASYLSGLKTCAVVDVGGTSTDISSICMGVPDLSDEGAVVGG 287 Query: 302 HKTYLRSLDVRTLAVAGGSMI 322 KT +R++ + T A G S + Sbjct: 288 WKTRVRAIRMETTATGGDSHV 308 >MA3498 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase, beta subunit Length = 640 Score = 117 bits (294), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 97/354 (27%), Positives = 176/354 (49%), Gaps = 31/354 (8%) Query: 5 QIRIGIDVGGTFTDAVAIDNETFEVLSKVKMPTTHHDE-RGVASGIVQIIQRIMSENGIQ 63 Q +GID GGT+TD++ I + +V+ K TT+ D G+ + I + ++ + E Sbjct: 2 QYSMGIDAGGTYTDSIIIRDSDGKVMDSNKALTTYPDLLAGIKNSIDGLDEKYLKE---- 57 Query: 64 PEDIKFIAHGTTQATNALLEGDVARVGILGMGTGLDARSARSETNVANIELAAGKYLTTY 123 +K ++ TT ATN +LE + VG++ +G + N + G + Sbjct: 58 ---VKMVSVSTTLATNTILEKNGYPVGLILVGD----YEIPEKMNAECYAVVKGGH---D 107 Query: 124 HKFIDSKELTREAIDDAIEQLLAEGAEVIVASEAYSVDDPANELRVIEAARSR-----GV 178 H + + L EA++ +E++ + S +S+ +PA+EL A ++R G+ Sbjct: 108 HHGTELEPLDMEAVEAFVEKV-KDRVSAFAVSSYFSIRNPAHEL----AIKTRIQELTGL 162 Query: 179 YATGGHEISQLYGLKTRTRTAVVNASLIPKMMETANMTEQAVKEAGITSQLMIMRCDGGV 238 GHE+S G R TA +NA LIP + + +K G+ ++L++++CDG V Sbjct: 163 PVVCGHELSLDLGAYERGVTAYLNAQLIPIASQFILSIREEIKRRGMDARLLMLKCDGSV 222 Query: 239 MSIDEVRKRPILTMXXXXXXXXXXXXMYEKISDGIFFEVGGTSVDISVIKNG--KVMIEN 296 + IDE ++PI ++ + +VGGTS D+S+++NG ++ E Sbjct: 223 VGIDEALEKPIESIFSGPAASLVGASHLSGLETCAVIDVGGTSTDVSMLENGLPELCPEG 282 Query: 297 AQVGGHKTYLRSLDVRTLAVAGGSMIQIGAGKITDVGPRSA---HIAGLEYECF 347 A VGG +T ++++ + T A+ G S + + KI ++GPR +A ++Y F Sbjct: 283 AVVGGWQTKVKAIRMETSAMGGDSHVWVKNMKI-NIGPRRVIPLCVAAVKYPGF 335 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.132 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 45,704,914 Number of extensions: 1765063 Number of successful extensions: 11689 Number of sequences better than 1.0e-05: 28 Number of HSP's gapped: 11629 Number of HSP's successfully gapped: 30 Length of query: 713 Length of database: 38,378,431 Length adjustment: 114 Effective length of query: 599 Effective length of database: 25,230,355 Effective search space: 15112982645 Effective search space used: 15112982645 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 119 (50.4 bits)