BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0032.p (267 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3679 [T] COG4753 Response regulator containing CheY-like recei... 154 2e-37 SP0661 [T] COG4753 Response regulator containing CheY-like recei... 151 8e-37 SPy1556 [T] COG4753 Response regulator containing CheY-like rece... 148 9e-36 SPy1062 [T] COG4753 Response regulator containing CheY-like rece... 126 3e-29 BH2109 [T] COG4753 Response regulator containing CheY-like recei... 107 1e-23 >BH3679 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 257 Score = 154 bits (388), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 85/254 (33%), Positives = 146/254 (57%), Gaps = 8/254 (3%) Query: 18 MGLHKLFLVEDEALIRSGLKHLVEEVIGGFKVVGEAENGRLALEALKSIKPDVLITDIRM 77 M + +V+DE R GLK +E V A + + A+ L D++ITDIRM Sbjct: 1 MKQQTVLIVDDEPRSRRGLKRTLEAWSPKLLNVQTAASAKEAITRLNEETIDLIITDIRM 60 Query: 78 NEMNGLEMIRRIRDQYPDLYILILSGYADFEYAKQAIKYGISDYLLKPVDRTELAQALG- 136 EM+GL +++ +++Q P +++S Y++FEYA A++ G+ YLLKPV + +L +A+ Sbjct: 61 PEMSGLHLVKALQEQNPKPVCIVISAYSEFEYAHTALELGVRHYLLKPVKKEKLIEAVES 120 Query: 137 --EFKRKHGSGGDISDSTGEGGTDNKGRQL-----IRKVKELVAQRLDQEISLQYMAEQV 189 + K++ G + E + +L I + + LDQ+I L+ +A+ V Sbjct: 121 ALQMKKEQDRFGQVEKVWDEKIDAIEKERLSSSDSIHEALHYIDAHLDQKIGLKDVAKSV 180 Query: 190 HLNHQYLSVLFKSETGQNFTDYVSQCRMNRAKQLLKETNLKIYEVAKLSGYLSSKHFMAV 249 HLN YLS LFK +T F++Y+++ R+ AK LL T+ I ++A+ GY ++K+F+ + Sbjct: 181 HLNPSYLSALFKEKTNMTFSEYLTRKRLQHAKHLLVSTDRPISDIAEKCGYQTAKYFIKI 240 Query: 250 FKDYTGRTPSQFRE 263 FK++ G TP+Q+R+ Sbjct: 241 FKEHEGMTPTQYRK 254 >SP0661 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 245 Score = 151 bits (382), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 90/249 (36%), Positives = 145/249 (58%), Gaps = 16/249 (6%) Query: 21 HKLFLVEDEALIRSGLKHLVEEVIGGFKVVGEAENGRLALEALKSIKPDVLITDIRMNEM 80 + + +VEDE L+R GL LV +++G+AENGR A E ++ PD+++TDI M + Sbjct: 3 YTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWELIQKQVPDIILTDINMPHL 62 Query: 81 NGLEMIRRIRDQYPDLYILILSGYADFEYAKQAIKYGISDYLLKPVDRTELAQALGEFKR 140 NG+++ +R+ YP ++++ L+GY DF+YA A+K G+ DYLLKP R ++ + LG+ K+ Sbjct: 63 NGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDDYLLKPFSRQDIEEMLGKIKQ 122 Query: 141 ------KHGSGGDISDSTGEGGTDNKGRQLIRKVKELVAQRLDQEISLQYMAEQVHLNHQ 194 K D+ + EG + +K++ +A D + SL+ +A + + Sbjct: 123 KLDKEEKEEQLQDLLTNRFEGN-------MAQKIQSHLA---DSQFSLKSLASDLGFSPT 172 Query: 195 YLSVLFKSETGQNFTDYVSQCRMNRAKQLLKETNLKIYEVAKLSGYLSSKHFMAVFKDYT 254 YLS L K E G F DY+ + R+ +AK LL T+LKIYE+A+ G+ +F FK Sbjct: 173 YLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGFEDMNYFTQRFKQIA 232 Query: 255 GRTPSQFRE 263 G TP QF++ Sbjct: 233 GVTPRQFKK 241 >SPy1556 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 246 Score = 148 bits (373), Expect = 9e-36, Method: Compositional matrix adjust. Identities = 89/248 (35%), Positives = 149/248 (60%), Gaps = 10/248 (4%) Query: 20 LHKLFLVEDEALIRSGLKHLVEEVIGGFKV--VGEAENGRLALEALKSIKPDVLITDIRM 77 ++ L +VEDE L+R G++ LV+ FK+ V EAENG+LA + + D+++TDI M Sbjct: 1 MYSLLIVEDEYLVRQGIRSLVD--FSQFKIDRVNEAENGQLAWDLFQKEPYDIVLTDINM 58 Query: 78 NEMNGLEMIRRIRDQYPDLYILILSGYADFEYAKQAIKYGISDYLLKPVDRTELAQALGE 137 ++NG+++ I+ + P +++ L+GY DF YA A+K G DYLLKP + ++ LG+ Sbjct: 59 PKLNGIQLAELIKQESPQTHLVFLTGYDDFNYALSALKLGADDYLLKPFSKADVEDMLGK 118 Query: 138 FKRKHGSGGDISDSTGE-GGTDNKGRQLIRKVKELVAQRL-DQEISLQYMAEQVHLNHQY 195 ++K ++S T + ++ + + + +RL D +++L+ +A+Q+ + Y Sbjct: 119 LRKKL----ELSKKTETIQELVEQPQKEVSAIAMAIHERLADSDLTLKSLAQQLGFSPNY 174 Query: 196 LSVLFKSETGQNFTDYVSQCRMNRAKQLLKETNLKIYEVAKLSGYLSSKHFMAVFKDYTG 255 LSVL K E G F DY+ Q R+ +AK LL +NLKIYE+A+ G+ +F FK G Sbjct: 175 LSVLIKKELGMPFQDYLVQERLKKAKLLLLTSNLKIYEIAEQVGFEDMNYFSQRFKQLVG 234 Query: 256 RTPSQFRE 263 TPSQ+++ Sbjct: 235 VTPSQYKK 242 >SPy1062 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 262 Score = 126 bits (317), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 75/252 (29%), Positives = 136/252 (53%), Gaps = 9/252 (3%) Query: 20 LHKLFLVEDEALIRSGLKHLVEEVIGGFKVVGEAENGRLALEALKSIKPDVLITDIRMNE 79 ++KL ++EDE +IR L++ ++ V+GEA++G+ +K +PD+++TDI M Sbjct: 1 MYKLVIIEDEHIIRKWLRYAIDYKALDILVIGEAKDGKEGAVLIKESQPDIVLTDINMPI 60 Query: 80 MNGLEMIRRIRDQYPDLYILILSGYADFEYAKQAIKYGISDYLLKPVDRTELAQAL---- 135 M +M +DQ +ILSGYADF A+ AI YG+ ++L KP+++ L + L Sbjct: 61 MTAFDMFEVTKDQ--TYAKIILSGYADFPNARSAIHYGVLEFLTKPIEKAALWECLQTII 118 Query: 136 GEFKRKHGSG--GDISDSTGEGGTDNKGRQLIRKVKELVAQRLDQEISLQYMAEQVHLNH 193 + +++ GS D ++ ++++ + E V +IS +A + + Sbjct: 119 AKIEKQKGSNQKTDACVYIPLPQMTDQIPEVVKDILEWVHAHFQDKISTSRLAHDLGYSE 178 Query: 194 QYLSVLFKSETGQNFTDYVSQCRMNRAKQLL-KETNLKIYEVAKLSGYLSSKHFMAVFKD 252 Y+ K +DY++Q R+N+A QL+ +E +L +YE+A+ G ++F VFK Sbjct: 179 SYIYQNIKKHLQMPLSDYINQYRINQAIQLMQQEPDLMVYEIAQAVGIYDYRYFDRVFKK 238 Query: 253 YTGRTPSQFRED 264 Y G+T F+E+ Sbjct: 239 YLGQTVKAFKEE 250 >BH2109 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 525 Score = 107 bits (268), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 49/117 (41%), Positives = 82/117 (70%), Gaps = 1/117 (0%) Query: 20 LHKLFLVEDEALIRSGLKHLVEEVIGGFKVVGEAENGRLALEALKSIKPDVLITDIRMNE 79 ++K+ L +DE +R GLK L+E G++V EA+NG A + ++ IKPD++ITDIRM Sbjct: 1 MYKVLLADDEFFVRHGLKSLIEWEKCGYEVAAEADNGEDAFKLVQEIKPDLVITDIRMPV 60 Query: 80 MNGLEMIRRIRDQYP-DLYILILSGYADFEYAKQAIKYGISDYLLKPVDRTELAQAL 135 +GL++I+++ + + +I+SGY DF YA++A+++G D++LKP+D+ EL AL Sbjct: 61 FDGLQLIQKVNETLSFNTKFIIISGYNDFSYAQKAVRFGAVDFVLKPIDQDELEAAL 117 Score = 71.2 bits (173), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 35/98 (35%), Positives = 61/98 (62%) Query: 165 IRKVKELVAQRLDQEISLQYMAEQVHLNHQYLSVLFKSETGQNFTDYVSQCRMNRAKQLL 224 I KVK + + ISL+ +A ++N Y+ LFK G F +Y+ + RMN AK+LL Sbjct: 423 IYKVKTYIENHYHENISLKSIASLFYMNPVYMGQLFKKTFGVYFKEYLLKLRMNEAKKLL 482 Query: 225 KETNLKIYEVAKLSGYLSSKHFMAVFKDYTGRTPSQFR 262 ++T++++YEVA+ G+ S+ +F+ F+ TP+++R Sbjct: 483 RQTDMRVYEVAERVGFGSTDYFVTQFEKINKMTPTEYR 520 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,235,240 Number of extensions: 813939 Number of successful extensions: 3853 Number of sequences better than 1.0e-05: 417 Number of HSP's gapped: 3867 Number of HSP's successfully gapped: 425 Length of query: 267 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 162 Effective length of database: 26,268,361 Effective search space: 4255474482 Effective search space used: 4255474482 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)