BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0017.p (523 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2109 [T] COG4753 Response regulator containing CheY-like recei... 160 6e-39 BH3842 [T] COG4753 Response regulator containing CheY-like recei... 140 5e-33 SP0661 [T] COG4753 Response regulator containing CheY-like recei... 92 2e-18 BH0483 [K] COG2207 AraC-type DNA-binding domain-containing proteins 92 3e-18 SPy1587 [T] COG4753 Response regulator containing CheY-like rece... 88 3e-17 >BH2109 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 525 Score = 160 bits (404), Expect = 6e-39, Method: Compositional matrix adjust. Identities = 137/540 (25%), Positives = 248/540 (45%), Gaps = 45/540 (8%) Query: 5 VLLVDDEAVDLEWLRRRVLSSGLNITVVGTASSGFGALKIMEEAQVDIILSDIRMPIMTG 64 VLL DDE L+ + V A +G A K+++E + D++++DIRMP+ G Sbjct: 4 VLLADDEFFVRHGLKSLIEWEKCGYEVAAEADNGEDAFKLVQEIKPDLVITDIRMPVFDG 63 Query: 65 TEFARRA-KAVSPKTKIVFISGHEDFSYAKEAIEINASGYLLKPVQDKDLYEMLGTLCAK 123 + ++ + +S TK + ISG+ DFSYA++A+ A ++LKP+ +L L L A Sbjct: 64 LQLIQKVNETLSFNTKFIIISGYNDFSYAQKAVRFGAVDFVLKPIDQDELEAALIKLAAT 123 Query: 124 MEQEREQ---------DRSFSEALSLVNKEL-LLRWFDEASPDSAEPHLKSILTPLLQKG 173 ++QE+ D+ F +L KE + RW A + H L Sbjct: 124 LDQEKATNSDQEKVLLDQIFIHSLGENKKENEVRRW--AAKLNINNRHFYYYL------- 174 Query: 174 AAAAIVEIDDLEWKMRDFSEEDARNITRQI--AGVIKSFVEEGKLGTWIAVHHTRFVILS 231 ++E+++L + F E + R++ V + E + + H ++++ Sbjct: 175 ----LLEVNNLSLD-KGFINEQYKEPIRKVIRETVARQAATEEYTAIYEQLDHAYGLLIT 229 Query: 232 SFPQERFLNQLEELIRK----VAEASPCTVTVGVGRYAADEAGLHESYRQAQAALSAKWL 287 S ++F Q+E L K + + + +TV VG D + + +SY A + + K++ Sbjct: 230 SEHLKKFNGQIEPLAEKLRKDIQDFTNIPLTVYVGAKTDDLSTIKKSYETANSLRNYKYI 289 Query: 288 LG-KNRLIRDTTQS--SPRGTPGAQIELTVERLLEAIIQYDLVSIDDHLLELF--TKDLP 342 KN I + T+S + + +T+ +E +V+I D + F K +P Sbjct: 290 FNEKNVFIHEQTESVDTTYDELNNHLFVTLMEQMEENNSDRIVNIIDKIFSEFQTKKFVP 349 Query: 343 SAGRKEVYELIIRITSKLHA--DXXXXXXXXXXXXXWESHQPDILFQFETIHDILSWXXX 400 SA + V + ++ + A WES+ F+ + + + Sbjct: 350 SAVKTSVNRCVHKVMQTVQAMEGDTGKIISAQAMTNWESYN----LTFDQLKQVFTKFVL 405 Query: 401 XXXXXXXXXYVKRQRQKRKLIDEIMRYVEEHLEQKITLKEVAAHFDFTPNYLGYLFKEEY 460 R+ + I ++ Y+E H + I+LK +A+ F P Y+G LFK+ + Sbjct: 406 EAAVIIQEL---RKENMKSDIYKVKTYIENHYHENISLKSIASLFYMNPVYMGQLFKKTF 462 Query: 461 GLPFSEYVNERKTSRVSELLRDPTLKIYEIAERMGYKNIIYFNRQFKQITGMTPGEYRKK 520 G+ F EY+ + + + +LLR +++YE+AER+G+ + YF QF++I MTP EYR K Sbjct: 463 GVYFKEYLLKLRMNEAKKLLRQTDMRVYEVAERVGFGSTDYFVTQFEKINKMTPTEYRNK 522 >BH3842 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 530 Score = 140 bits (353), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 149/561 (26%), Positives = 242/561 (43%), Gaps = 91/561 (16%) Query: 3 INVLLVDDEAVDLEWLRRRVLSSGLNITVVGTASSGFGALKIMEEAQVDIILSDIRMPIM 62 INVL+VDDE ++ E L+ L NI +VG AS+G A++ E Q D+++ DI+MP + Sbjct: 2 INVLIVDDEQIEREGLKL-FLKDRPNIHIVGEASNGRQAIEKALETQPDLMMMDIKMPGV 60 Query: 63 TGTEFARRAKAVSPKTKIVFISGHEDFSYAKEAIEINASGYLLKPVQDKDLYEMLGTLCA 122 G + + K P+T+ + +S + F YA+E + YLLKP +D+ L + Sbjct: 61 DGVQAVKEIKQQLPETEFIMVSAFDTFEYAREVMRYGVKEYLLKPTTKEDVLTALDRIVG 120 Query: 123 KMEQEREQ-------DRSFSEALSLVNKELLL----------RWFDEASPDSAEPHLKSI 165 ++EQ RE+ + + S V E +L W + E ++ Sbjct: 121 EIEQAREEKNDQYKLQQRLEQISSFVQTESILYLLMDHIQDYEWMEWNEWLRTEQEGYAV 180 Query: 166 LTPLLQKGAAAAIVEIDDLEWKMRDFSEEDARNITRQIAGVIKSFVEEGKLGTWIAVHHT 225 + Q+G I + + +W R I I + + +G +I HH Sbjct: 181 VFSFHQQG--KEIRKTEKQQWHAR-------------IKKEIGLQLPKSTVGPFIG-HHL 224 Query: 226 RFVILSSFPQERFLNQLE------ELIRKVAEA-SPCTVTVGVGRYAADEAGLH---ESY 275 ++L+ E + + E +L+ KV +A P V GVG LH SY Sbjct: 225 PILMLTE--NENGMTKQEREQVVIQLLHKVKQAVQPAKVKTGVGGIYR----LHFFSTSY 278 Query: 276 RQAQAAL--------SAKWLLGKNRLIRDTTQSSPRGTPGAQIELTVER---LLEAIIQY 324 R+A AL ++ + KN IR A +L +ER L+EAI Sbjct: 279 REALLALDIIYDHKQASYYFYDKNVKIR------------ADEQLPIERENKLIEAIKSG 326 Query: 325 D----LVSIDDHLLELF--TKDLPSAGRKEVYELIIRITSKLHADXXXXXXXXXXXXXWE 378 D L + D ++ + +K RK + +L I + L+ WE Sbjct: 327 DGEKALQAFDHYVQAILRCSKHPLHDMRKYLQQLFIILGRLLNE----------MGLHWE 376 Query: 379 SHQPDILFQFETIHDILSWXXXXXXXXXXXXYVKRQRQKRKLIDEIMRYVEEHLEQKITL 438 + Q +F T+H + R ++ L+ YV +H + +TL Sbjct: 377 TAQD--FARFSTVHQVTEHGKSEILHIVRTLQEWRTTDEQSLMLLARDYVHDHFREPLTL 434 Query: 439 KEVAAHFDFTPNYLGYLFKEEYGLPFSEYVNERKTSRVSELLRDPTLKIYEIAERMGYKN 498 ++A H +P Y+ LFKE + L FSEYV + + +LL + EI +GYK+ Sbjct: 435 DDLAKHIGLSPYYVSKLFKERFDLTFSEYVTQLRVEHAKQLLSLTQKSLKEITYEIGYKD 494 Query: 499 IIYFNRQFKQITGMTPGEYRK 519 YF+R FK++TG +P +YRK Sbjct: 495 PNYFSRVFKKLTGKSPSDYRK 515 >SP0661 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 245 Score = 92.0 bits (227), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 42/132 (31%), Positives = 83/132 (62%) Query: 1 MSINVLLVDDEAVDLEWLRRRVLSSGLNITVVGTASSGFGALKIMEEAQVDIILSDIRMP 60 M+ +L+V+DE + + L + V + ++ ++G A +G A +++++ DIIL+DI MP Sbjct: 1 MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWELIQKQVPDIILTDINMP 60 Query: 61 IMTGTEFARRAKAVSPKTKIVFISGHEDFSYAKEAIEINASGYLLKPVQDKDLYEMLGTL 120 + G + A + P+ +VF++G++DF YA A+++ YLLKP +D+ EMLG + Sbjct: 61 HLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDDYLLKPFSRQDIEEMLGKI 120 Query: 121 CAKMEQEREQDR 132 K+++E ++++ Sbjct: 121 KQKLDKEEKEEQ 132 Score = 76.6 bits (187), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 38/93 (40%), Positives = 63/93 (67%), Gaps = 1/93 (1%) Query: 428 VEEHL-EQKITLKEVAAHFDFTPNYLGYLFKEEYGLPFSEYVNERKTSRVSELLRDPTLK 486 ++ HL + + +LK +A+ F+P YL L K+E GLPF +Y+ + + LL LK Sbjct: 149 IQSHLADSQFSLKSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLK 208 Query: 487 IYEIAERMGYKNIIYFNRQFKQITGMTPGEYRK 519 IYEIAE++G++++ YF ++FKQI G+TP +++K Sbjct: 209 IYEIAEKVGFEDMNYFTQRFKQIAGVTPRQFKK 241 >BH0483 [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 769 Score = 91.7 bits (226), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 91/381 (23%), Positives = 171/381 (44%), Gaps = 25/381 (6%) Query: 154 SPDSAEPHLKSILTPLLQKGAAAAIVEIDDLEWKMRDFSEEDARNITRQIAGVIKSFVEE 213 SP E ++ P + ++ID LE +++E+ + I ++++ + E Sbjct: 395 SPSEIETKYETFQYPKDWRQLHVLTLQIDSLEGT--KYAKEEKDVLLFSINNLVETLIPE 452 Query: 214 GKLGTWIAVHHTR-FVILSSFPQER----FLNQ-LEELIRKVAEASPCTVTVGVGRYAAD 267 + T +++T ++L++ QE+ F+ + E ++ +V + +V++GV D Sbjct: 453 VERLTPALLNNTVVLILLTNVDQEQKRDGFVREKAEAIMNEVRQQLDISVSIGVSLPYDD 512 Query: 268 EAGLHESYRQAQAALSAKWLLGKNRLIRDTTQSSPRGTPGAQIELTVERLLEAIIQYDLV 327 E+Y + AL + LG +I + + + L +A+ D Sbjct: 513 ITKTQEAYDEGIEALKYRIKLGCQSVIFFSNLAHTHSFHTYYPKQQENELFDALKLGDKS 572 Query: 328 SIDDHLLEL----FTKDL-PSAGRKEVYELI---IRITSKLHADXXXXXXXXXXXXXWES 379 ++ DHL L F KDL PS + + L+ I++ L D Sbjct: 573 NVKDHLDALIDGIFQKDLHPSQYQISLLRLLNNTIQLLQTLGIDSVDVYDKKTLF----- 627 Query: 380 HQPDILFQFETIHDILSWXXXXXXXXXXXXYVKRQ-RQKRKLIDEIMRYVEEHLEQKITL 438 D LF +TI ++ W +R Q R + D+I+ ++ + ++TL Sbjct: 628 ---DQLFSLQTIDEVKQWFFTKLIAPSVHTVAERMDSQYRTISDQIIHIIQNEFDMELTL 684 Query: 439 KEVAAHFDFTPNYLGYLFKEEYGLPFSEYVNERKTSRVSELLRDPTLKIYEIAERMGYKN 498 + +A + PNYL +F++E + FSEY++ + + + L + ++ I EIAER+ Y N Sbjct: 685 ESIAERLHYNPNYLSSIFRKETNISFSEYLSSYRLAMAKKWLLETSMPIREIAERLCYGN 744 Query: 499 IIYFNRQFKQITGMTPGEYRK 519 F R FK+ GMTPG+YR+ Sbjct: 745 SQNFIRSFKKKEGMTPGKYRQ 765 >SPy1587 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 494 Score = 88.2 bits (217), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 51/125 (40%), Positives = 75/125 (60%), Gaps = 7/125 (5%) Query: 5 VLLVDDEAVDLEWLRRRVLSSGLNITVVGTASSGFGALKIMEEAQVDIILSDIRMPIMTG 64 VLLVDDE + L+ L + L VV TA SG AL + + VD+++SD+ MP MTG Sbjct: 4 VLLVDDEYMILQGLTMIIDWQALGFEVVQTARSGKEALAYLTQYPVDVMISDVTMPGMTG 63 Query: 65 TEFARRAKAVSPKTKIVFISGHEDFSYAKEAIEINASGYLLKPVQDKDLYEMLGTLCAKM 124 + AK P+ + + +SG+++FSY ++A+E+ GYLLKPV DK L AKM Sbjct: 64 LDLIEAAKTYHPQLQTLILSGYQEFSYVQKAMELETKGYLLKPV-DK------AELQAKM 116 Query: 125 EQERE 129 +Q ++ Sbjct: 117 KQFKD 121 Score = 64.3 bits (155), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 33/103 (32%), Positives = 54/103 (52%) Query: 420 LIDEIMRYVEEHLEQKITLKEVAAHFDFTPNYLGYLFKEEYGLPFSEYVNERKTSRVSEL 479 L+ E + + + Q++TLK +A YLG FK E F++Y+N + + +L Sbjct: 382 LVSETIDCIRKEYHQELTLKAIADRLHVNGVYLGQCFKNETERSFTQYLNHVRIQKAQQL 441 Query: 480 LRDPTLKIYEIAERMGYKNIIYFNRQFKQITGMTPGEYRKKHK 522 L I EIA GY YF + FK++ G++P E+R ++K Sbjct: 442 LLYTNQSINEIAYETGYNTNHYFIKMFKKLNGLSPKEFRDRYK 484 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 36,140,820 Number of extensions: 1415655 Number of successful extensions: 7565 Number of sequences better than 1.0e-05: 942 Number of HSP's gapped: 7699 Number of HSP's successfully gapped: 953 Length of query: 523 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 412 Effective length of database: 25,576,357 Effective search space: 10537459084 Effective search space used: 10537459084 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)