BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0014.p (457 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb21655 [G] COG3250 Beta-galactosidase/beta-glucuronidase 120 3e-27 TM1193 [G] COG3250 Beta-galactosidase/beta-glucuronidase 116 9e-26 TM1062 [G] COG3250 Beta-galactosidase/beta-glucuronidase 112 1e-24 STM1062 [R] COG0488 ATPase components of ABC transporters with d... 112 1e-24 ZebgA [G] COG3250 Beta-galactosidase/beta-glucuronidase 98 2e-20 >SMb21655 [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 755 Score = 120 bits (302), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 115/436 (26%), Positives = 187/436 (42%), Gaps = 74/436 (16%) Query: 7 RSRSRFDENWSFYKGDIRIPHAVKAGMTGGITDCGSLQEGEWLDIAFNDKGMGHQQLEWK 66 RS + F+++W F +G D G+L+ G+ + + N Sbjct: 3 RSVTSFNDSWVFSEG-------------FDAADAGTLRAGQPISLPHN------------ 37 Query: 67 PVSLPHDWCVEQQYVQDASLGSRDGSHGYLPGGTGFYRKTFELPPEAADRKWTVTFDGAS 126 V LP ++ E+ Y + + Y++ P+ + R+ ++ FD A Sbjct: 38 AVELPFNYFDERCYQRAFT-----------------YQRVLAWRPDFSGREVSLVFDAAM 80 Query: 127 GLSTVWVNGHLIGEHRGGYIGFSYDLTDVLRYGAEGRNVILVKVEALENEGW-------- 178 + V++NG I HR GY F LTD L EG N+I VK++ EN Sbjct: 81 ADAVVYLNGEEIVAHRDGYTPFEARLTDRL---LEGDNLITVKIDGSENPEIPPFGGRID 137 Query: 179 WYEGCGIYRHVWLEQTDRLHVAEYGTYITTPEAEKEQALVHISTRICNEYTESRAVSLHT 238 + GIYR VWL+ TD + +A I T + + V + + N S + ++ Sbjct: 138 YLTYAGIYRDVWLKVTDPVSIAN--IKIETRDVLSDTKAVSLRCDLSNPQGLSFSGTISA 195 Query: 239 EVYNAAGEQVCAKAEQAFADWYGETELEQMFRLEHPALWSPETPHLYKAVSILKVE---G 295 + NAAGE + A + G++ +M L+ +LW + P LY +++VE G Sbjct: 196 LLKNAAGEVLAEVAGET----TGQSLAFEMDGLKGLSLWDIDDPVLY----VIEVELRTG 247 Query: 296 TEVDRYETPFGIRSIRFDPEEGFFLNGEPLLIKGTCNHQDFAGVGVALPDSLIEYKLRLL 355 D + FG R+ F EGF LNG PL I+G HQ F VG A+ + E ++ Sbjct: 248 QGSDCFAAHFGFRTAEFT-TEGFRLNGRPLKIRGLNRHQSFPYVGYAMGRTAQERDADIM 306 Query: 356 K-EMGSNAYRSAHHPPTPEXXXXXXXXXXXVMDENRKLDS------SRSGLDQLKRMLFR 408 K + N R++H+P + V +E + + ++RM+ R Sbjct: 307 KHRLHCNLVRTSHYPQSKWFLDHCDRIGLLVFEEIPGWQHIGGEEWKQEAIRNVRRMIER 366 Query: 409 DRNHPSVIIWNLENEE 424 D NHPS++IW + E Sbjct: 367 DWNHPSIVIWGVRINE 382 >TM1193 [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 1087 Score = 116 bits (290), Expect = 9e-26, Method: Compositional matrix adjust. Identities = 96/348 (27%), Positives = 155/348 (44%), Gaps = 41/348 (11%) Query: 100 TGFYRKTFELPPEAADRKWTVTFDGASGLSTVWVNGHLIGEHRGGYIGFSYDLTDVLRYG 159 TG YR+ E+P + ++ + F+G +WVNG IG + + LTDVLR Sbjct: 114 TGVYRRWIEIPEDWFKKEIFLHFEGVRSFFYLWVNGKKIGFSKDSCTPAEFRLTDVLR-- 171 Query: 160 AEGRNVILVKV------EALENEG-WWYEGCGIYRHVWLEQTDRLHVAEYGTYITTPEAE 212 G+N+I V+V LE++ WW+ GIYR V+L + H+ + E Sbjct: 172 -PGKNLITVEVLKWSDGSYLEDQDMWWF--AGIYRDVYLYALPKFHIRDVFVRTDLDENY 228 Query: 213 KE-QALVHISTRICNEYTESRAVSLHTEVYNAAGEQVCAKAEQAFADWYGETELEQMFRL 271 + + + + R E E L + G++ E + + L F + Sbjct: 229 RNGKIFLDVEMRNLGEEEEK---DLEVTLITPDGDEKTLVKETVKPE---DRVLSFAFDV 282 Query: 272 EHPALWSPETPHLYKAVSILKVEGTEVDRYETPFGIRSIRFDPEEGFFLNGEPLLIKGTC 331 + P WS ETPHLY +LK++ E D + FG R I + NG+PL IKG Sbjct: 283 KDPKKWSAETPHLY----VLKLKLGE-DEKKVNFGFRKIEIK-DGTLLFNGKPLYIKGVN 336 Query: 332 NHQDFAGVGVALPDSLIEYKLRLLKEMGSNAYRSAHHPPTPEXXXXXXXXXXXVMDE--- 388 H+ G A+ + ++L+K+ N R++H+P + V+DE Sbjct: 337 RHEFDPDRGHAVTVERMIQDIKLMKQHNINTVRTSHYPNQTKWYDLCDYFGLYVIDEANI 396 Query: 389 -------------NRKLDSSRSGLDQLKRMLFRDRNHPSVIIWNLENE 423 + + ++ D++KRM+ RD+NHPS+I W+L NE Sbjct: 397 ESHGIDWDPEVTLANRWEWEKAHFDRIKRMVERDKNHPSIIFWSLGNE 444 >TM1062 [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 563 Score = 112 bits (280), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 105/390 (26%), Positives = 178/390 (45%), Gaps = 66/390 (16%) Query: 66 KPVSLPHDWCVEQQYVQDASLGSRDGSHGYLPGGTGFYRKTFELPPEAADRKWTVTFDGA 125 +P+++P W +QY QD G Y+ TF +P E + + + F Sbjct: 28 RPIAVPGSW--NEQY-QDLCYEE----------GPFTYKTTFYVPKELSQKHIRLYFAAV 74 Query: 126 SGLSTVWVNGHLIGEHRGGYIGFSYDLTDVLRYGAEGRNVIL---VKVEALENE------ 176 + V++NG +GE+ Y+ F D+T ++ G V++ +KV ++ Sbjct: 75 NTDCEVFLNGEKVGENHIEYLPFEVDVTGKVKSGENELRVVVENRLKVGGFPSKVPDSGT 134 Query: 177 ---GWW----------YEGCGIYRHVWLEQTDRLHVAEYGTYITTPEAEKEQALVHISTR 223 G++ + GI R V +E TD H ++ T E+E E+ L + + Sbjct: 135 HTVGFFGSFPPANFDFFPYGGIIRPVLIEFTD--HARILDIWVDTSESEPEKKLGKVKVK 192 Query: 224 ICNEYTESRAVSLHTEVYNAAGEQVCAK-AEQAFADWYGETELEQMFRLEHPALWSPETP 282 I E +E A G+++ K E+ +E F LE+ WS E P Sbjct: 193 I--EVSEE-----------AVGQEMTIKLGEEEKKIRTSNRFVEGEFILENARFWSLEDP 239 Query: 283 HLYKAVSILKVEGTEVDRYETPFGIRSIRFDPEEGFFLNGEPLLIKGTCNHQDFAGVGVA 342 +LY LKVE E D Y GIR+I +D E+ +LNG+P+ +KG H++F +G Sbjct: 240 YLYP----LKVE-LEKDEYTLDIGIRTISWD-EKRLYLNGKPVFLKGFGKHEEFPVLGQG 293 Query: 343 LPDSLIEYKLRLLKEMGSNAYRSAHHPPTPEXXXXXXXXXXXVMDENRKL---------D 393 L+ LLK + +N++R++H+P + E V+DE + + Sbjct: 294 TFYPLMIKDFNLLKWINANSFRTSHYPYSEEWLDLADRLGILVIDEAPHVGITRYHYNPE 353 Query: 394 SSRSGLDQLKRMLFRDRNHPSVIIWNLENE 423 + + D ++RM+ R +NHPSVI+W++ NE Sbjct: 354 TQKIAEDNIRRMIDRHKNHPSVIMWSVANE 383 >STM1062 [R] COG0488 ATPase components of ABC transporters with duplicated ATPase domains Length = 635 Score = 112 bits (280), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 19/390 (4%), Positives = 48/390 (12%), Gaps = 66/390 (16%) Query: 66 KPVSLPHDWCVEQQYVQDASLGSRDGSHGYLPGGTGFYRKTFELPPEAADRKWTVTFDGA 125 + ++L + + Sbjct: 28 DNERVCLVG--RNGA-GKSTLMK----------ILNREQGLDDGRIIYEQDLIVARLQQD 74 Query: 126 SGLSTVWVNGHLIGEHRGGYIGFSYDLTDVLRYGAEGRNVIL---VKVEALENE------ 176 + + E + ++ R + + + + Sbjct: 75 PPRNIAGSVYDFVAEGIEEQAEYLKRYHEISRLVMTDPSEKNLNEMARVQEQLDHHNLWQ 134 Query: 177 ---GWW----------YEGCGIYRHVWLEQTDRLHVAEYGTYITTPEAEKEQALVHISTR 223 WL + + ++ + I T Sbjct: 135 LENRINEVLAQLGLDPNAALSSLSGGWLRKAA--LGRALVSNPRVLLLDEPTNHLDIETI 192 Query: 224 ICNEYTESRAVSLHTEVYNAAGEQVCAK-AEQAFADWYGETELEQMFRLEHPALWSPETP 282 + + Sbjct: 193 D--WLEGF-----------LKTFNGTIIFISHDRSFIRNMATRIVDLDRGKLVTYPGNYD 239 Query: 283 HLYKAVSILKVEGTEVDRYETPFGIRSIRFDPEEGFFLNGEPLLIKGTCNHQDFAGVGVA 342 K E EE + G Sbjct: 240 QYLL----EKEE-ALRVEELQNAEFDRKLAQ-EEVWIRQGIKARRTRNEGRVRALKAMRR 293 Query: 343 LPDSLIEYKLRLLKEMGSNAYRSAHHPPTPEXXXXXXXXXXXVMDENRKL---------D 393 E ++ + Sbjct: 294 ERSERREVMGTAKMQVEEATRSGKIVFEMENVDYQVEGKQLVKDFSAQVQRGDKIALIGP 353 Query: 394 SSRSGLDQLKRMLFRDRNHPSVIIWNLENE 423 + LK ML + + I + E Sbjct: 354 NGCGKTTLLKLMLGQLQADSGRIHVGTKLE 383 >ZebgA [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 1042 Score = 98.2 bits (243), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 94/389 (24%), Positives = 162/389 (41%), Gaps = 38/389 (9%) Query: 64 EWKPVSLPHDWCVE----QQYVQDASLGSRDGSHGYLPGGTGFYRKTFELPPEAADRKWT 119 +W +++P W +E QY + D TG Y++ F L ++ Sbjct: 82 DWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVPFVPSDNPTGAYQRIFTLSDGWQGKQTL 141 Query: 120 VTFDGASGLSTVWVNGHLIGEHRGGYIGFSYDLTDVLRYGAEGRNVILVKV------EAL 173 + FDG V+VNG +G +G + +D++ +++ G N++ V+V + Sbjct: 142 IKFDGVETYFEVYVNGQYVGFSKGSRLTAEFDISAMVK---TGDNLLCVRVMQWADSTYV 198 Query: 174 ENEGWWYEGCGIYRHVWLEQTDRLHVAEYGTYITTPEAEKEQALVHISTRICNEYTESRA 233 E++ W+ GI+R V+L H+ ++ EA + L S + E + Sbjct: 199 EDQDMWW-SAGIFRDVYLVGKHLTHINDFTVRTDFDEAYCDATL---SCEVVLENLAASP 254 Query: 234 VSLHTEVYNAAGEQVCAKAEQAFADWYGETELEQMFRLEHPALWSPETPHLYKAVSILK- 292 V E GE+V + T F +E P WS E+P+LY V LK Sbjct: 255 VVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASFAFTVEQPQQWSAESPYLYHLVMTLKD 314 Query: 293 VEGTEVDRYETPFGIRSIRFDPEEGFF-LNGEPLLIKGTCNHQDFAGVGVALPDSLIEYK 351 G ++ G R I+ +G F +N +++ G H + G A+ +E Sbjct: 315 ANGNVLEVVPQRVGFRDIKV--RDGLFWINNRYVMLHGVNRHDNDHRKGRAVGMDRVEKD 372 Query: 352 LRLLKEMGSNAYRSAHHPPTPEXXXXXXXXXXXVM--------------DENRKLDS--- 394 L+L+K+ N+ R+AH+P P VM D +R D Sbjct: 373 LQLMKQHNINSVRTAHYPNDPRFYELCDIYGLFVMAETDVESHGFANVGDISRITDDPQW 432 Query: 395 SRSGLDQLKRMLFRDRNHPSVIIWNLENE 423 + ++++ R + +NHPS+IIW+L NE Sbjct: 433 EKVYVERIVRHIHAQKNHPSIIIWSLGNE 461 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 36,817,674 Number of extensions: 1604473 Number of successful extensions: 2844 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 2805 Number of HSP's successfully gapped: 16 Length of query: 457 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 347 Effective length of database: 25,691,691 Effective search space: 8915016777 Effective search space used: 8915016777 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)