BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0340.0034.p (129 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO2333 [P] COG2076 Membrane transporters of cations and cationi... 74 4e-14 SMc01523 [P] COG2076 Membrane transporters of cations and cation... 73 9e-14 BS_ebrA [P] COG2076 Membrane transporters of cations and cationi... 67 4e-12 Z1870 [P] COG2076 Membrane transporters of cations and cationic ... 66 1e-11 ECs1614 [P] COG2076 Membrane transporters of cations and cationi... 66 1e-11 >YPO2333 [P] COG2076 Membrane transporters of cations and cationic drugs Length = 112 Score = 74.3 bits (181), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 35/84 (41%), Positives = 58/84 (69%) Query: 3 YLLLAVSIASELLGTSMLKASQGFTRLVPSIVTVVAFVCSFYFLSISLKSIPLNTAYAIW 62 ++ L ++I +E++ T+MLKAS GF+RLVPS+V V+ + +F+ LS +K++PL AYAIW Sbjct: 6 FVYLFMAIIAEVVATTMLKASDGFSRLVPSVVVVIGYGIAFWGLSQVVKTMPLGIAYAIW 65 Query: 63 SGLGXXXXXXXXXXXWKEKINTAS 86 SGLG +++K++ A+ Sbjct: 66 SGLGIVLVSIAATFMYQQKLDWAA 89 >SMc01523 [P] COG2076 Membrane transporters of cations and cationic drugs Length = 113 Score = 73.2 bits (178), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 32/81 (39%), Positives = 54/81 (66%) Query: 3 YLLLAVSIASELLGTSMLKASQGFTRLVPSIVTVVAFVCSFYFLSISLKSIPLNTAYAIW 62 Y +L ++I E+LGTS ++A+Q FTRL P+++ V+ + +F+FLS +L+ IP+ AYA+W Sbjct: 7 YTVLVIAIVFEVLGTSAMQAAQHFTRLTPTVLMVLCYAVAFFFLSYALRYIPVGIAYALW 66 Query: 63 SGLGXXXXXXXXXXXWKEKIN 83 SGLG + +K++ Sbjct: 67 SGLGIVLISIAGYVVFGQKLD 87 >BS_ebrA [P] COG2076 Membrane transporters of cations and cationic drugs Length = 105 Score = 67.4 bits (163), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 32/83 (38%), Positives = 49/83 (59%) Query: 1 MAYLLLAVSIASELLGTSMLKASQGFTRLVPSIVTVVAFVCSFYFLSISLKSIPLNTAYA 60 + Y+ L ++I SE +G +MLK S GF + PS + V+ + +FY LS++L IPL+ +YA Sbjct: 3 IGYIFLTIAICSESIGAAMLKVSDGFKKWKPSALVVIGYSLAFYMLSLTLNHIPLSLSYA 62 Query: 61 IWSGLGXXXXXXXXXXXWKEKIN 83 WSG G +KE +N Sbjct: 63 TWSGAGTVLTTVIGVKWFKEDLN 85 >Z1870 [P] COG2076 Membrane transporters of cations and cationic drugs Length = 110 Score = 66.2 bits (160), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 30/64 (46%), Positives = 47/64 (73%) Query: 3 YLLLAVSIASELLGTSMLKASQGFTRLVPSIVTVVAFVCSFYFLSISLKSIPLNTAYAIW 62 Y+ L +I +E++GT+++K S+GFTRL PS+ T++ + SF+ L+ +L IP AYAIW Sbjct: 4 YIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASFWLLAQTLAYIPTGIAYAIW 63 Query: 63 SGLG 66 SG+G Sbjct: 64 SGVG 67 >ECs1614 [P] COG2076 Membrane transporters of cations and cationic drugs Length = 110 Score = 66.2 bits (160), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 30/64 (46%), Positives = 47/64 (73%) Query: 3 YLLLAVSIASELLGTSMLKASQGFTRLVPSIVTVVAFVCSFYFLSISLKSIPLNTAYAIW 62 Y+ L +I +E++GT+++K S+GFTRL PS+ T++ + SF+ L+ +L IP AYAIW Sbjct: 4 YIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASFWLLAQTLAYIPTGIAYAIW 63 Query: 63 SGLG 66 SG+G Sbjct: 64 SGVG 67 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.126 0.352 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 5,317,504 Number of extensions: 115551 Number of successful extensions: 290 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 290 Number of HSP's successfully gapped: 32 Length of query: 129 Length of database: 38,378,431 Length adjustment: 94 Effective length of query: 35 Effective length of database: 27,537,035 Effective search space: 963796225 Effective search space used: 963796225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 109 (46.6 bits)