BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0340.0009.p (809 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_nasD [C] COG1251 NAD(P)H-nitrite reductase 974 0.0 PA1781 [C] COG1251 NAD(P)H-nitrite reductase 826 0.0 BS_nasB [C] COG1251 NAD(P)H-nitrite reductase 634 0.0 RSp1222 [C] COG1251 NAD(P)H-nitrite reductase 471 e-132 RSp1220 [C] COG1251 NAD(P)H-nitrite reductase 375 e-103 >BS_nasD [C] COG1251 NAD(P)H-nitrite reductase Length = 346 Score = 974 bits (2519), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/803 (0%), Positives = 0/803 (0%), Gaps = 8/803 (0%) Query: 5 RKKLVLVGNGMAGVRAIEHLLKLSPEAYEITIFGSEPHPNYNRIMLSSVLAGGSSMEEII 64 ++ ++ + + ++ + + E Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 65 INDLEWYHSHGIRLYMGHTITSIDTAKRKVYSDKGIVMPYDELILATGSNPFMLPLPGAE 124 + + + + ++ + P L L + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 125 KEGVIAFRDIKDCQIMQETSQSYRKAVVIXXXXXXXXXXXXXXXXXMEVSVVHIHPYIME 184 + + ++ + + + +I Sbjct: 123 TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNET 182 Query: 185 RQLDEPASVMLRKELEEQGMKFLLNKQSEAILGKKRVKGLLFADGEIADADLIVMAVGIK 244 + K + + + + + +G A + + Sbjct: 183 AFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLT 242 Query: 245 PNVELAQKSGVQTNRGIIVNDYMETNIPGIYAVGECAEHRGIAYGLVAPLYEQGAVLAKR 304 K N R Sbjct: 243 GKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFV 302 Query: 305 LAGVPTDGYPGSVTSTKLKVSGVDVFSAGQFTERPGTRSLRYQDEIEGIYKKLVIQDDQL 364 Sbjct: 303 362 Query: 365 VGAVLFGDTGDGAQLFSMXXXXXXXXXXXXXXXXXVSSDALASPKGSRLEG-MADDEIIC 423 Sbjct: 363 ---- 418 Query: 424 GCNGVSKGAIAEAIQSGGCTSVGQIKACTKASASCGGCKPLVEGLLQLYAGDN--VVTVK 481 Sbjct: 419 478 Query: 482 EGICGCTTLGRDEIVAEIRRMELKTVKEVMNVLEWNTEEGCAKCRPSLNYYLGMLWPEEY 541 Sbjct: 479 538 Query: 542 IDEKESRITNERYHANIQKDGTYSVVPRIYGGVTSPAELMKIAEVAAKFEVPLVKFTGGQ 601 Sbjct: 539 598 Query: 602 RLDLLGVKKEDLPKMWEELDMPSGHAYGKTLRTVKTCVGSTFCRFGTQDAMGMGIRLEKA 661 Sbjct: 599 658 Query: 662 FERLNTPGKVKLAVSGCPRNCAEATIKDFGVVAIDGGWELHIGGNGGVHVRATDLLCVVK 721 Sbjct: 659 718 Query: 722 TDDEVIEWASAFLQYYREQAGWNERTAQWVERVGLDSIKEVLEVRENRLALQERIHTALS 781 Sbjct: 719 778 Query: 782 VTTDPWKQIVNEPELRKN-FEQL 803 Sbjct: 779 801 >PA1781 [C] COG1251 NAD(P)H-nitrite reductase Length = 346 Score = 826 bits (2133), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/805 (0%), Positives = 0/805 (0%), Gaps = 7/805 (0%) Query: 7 KLVLVGNGMAGVRAIEHLLKLSPEAYEITIFGSEPHPNYNRIMLSSVLAGGSSMEEIIIN 66 ++ ++ + + ++ + + E Sbjct: 5 RIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGL 64 Query: 67 DLEWYHSHGIRLYMGHTITSIDTAKRKVYSDKGIVMPYDELILATGSNPFMLPLPGAEKE 126 + + + + ++ + P L L + + Sbjct: 65 TETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTD 124 Query: 127 GVIAFRDIKDCQIMQETSQSYRKAVVIXXXXXXXXXXXXXXXXXMEVSVVHIHPYIMERQ 186 + ++ + + + +I Sbjct: 125 YLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAF 184 Query: 187 LDEPASVMLRKELEEQGMKFLLNKQSEAIL--GKKRVKGLLFADGEIADADLIVMAVGIK 244 + K + + +A + L + K Sbjct: 185 GVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGK 244 Query: 245 PNVELAQKSGVQTNRGIIVNDYMETNIPGIYAVGECAEHRGIAYGLVAPLYEQGAVLAKR 304 T E + A + A G+ +A + + Sbjct: 245 YLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVAS 304 Query: 305 LAGVPTDGYPGSVTSTKLKVSGVDVFSAGQFTERPGTRSLRYQDEIEGIYKKLVIQDDQL 364 Sbjct: 305 364 Query: 365 VGAVLFGDTGDGAQLFSMXXXXXXXXXXXXXXXXXVSSDALASPKG-SRLEGMADDEIIC 423 Sbjct: 365 424 Query: 424 GCNGVSKGAIAEAIQSGGCTSVGQIKACTKASASCGGCKPLVEGLLQLYAG---DNVVTV 480 Sbjct: 425 484 Query: 481 KEGICGCTTLGRDEIVAEIRRMELKTVKEVMNVLEWNTEEGCAKCRPSLNYYLGMLWPEE 540 Sbjct: 485 544 Query: 541 YIDEKESRITNERYHANIQKDGTYSVVPRIYGGVTSPAELMKIAEVAAKFEVPLVKFTGG 600 Sbjct: 545 604 Query: 601 QRLDLLGVKKEDLPKMWEELDMPSGHAYGKTLRTVKTCVGSTFCRFGTQDAMGMGIRLEK 660 Sbjct: 605 664 Query: 661 AFERLNTPGKVKLAVSGCPRNCAEATIKDFGVVAIDGGWELHIGGNGGVHVRATDLLCVV 720 Sbjct: 665 724 Query: 721 KTDDEVIEWASAFLQYYREQAGWNERTAQWVERVGLDSI-KEVLEVRENRLALQERIHTA 779 Sbjct: 725 784 Query: 780 LSVTTDPWKQIVNEPELRKNFEQLS 804 Sbjct: 785 809 >BS_nasB [C] COG1251 NAD(P)H-nitrite reductase Length = 346 Score = 634 bits (1634), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/787 (0%), Positives = 0/787 (0%), Gaps = 34/787 (4%) Query: 5 RKKLVLVGNGMAGVRAIEHLLKLSPEAYEITIFGSEPHPNYNRIMLSSVLAGGSSMEEII 64 ++ ++ + + ++ + + E Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 65 INDLEWYHSHGIRLYMGHTITSIDTAKRKVYSDKGIVMPYDELILATGSNPFMLPLPGAE 124 + + + + ++ + P L L + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 125 KEGVIAFRDIKDCQIMQETSQSYRKAVVIXXXXXXXXXXXXXXXXXMEVSVVHIHPYIME 184 + + ++ + + + +I Sbjct: 123 TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNET 182 Query: 185 RQLDEPASVMLRKELEEQGMKFLLNKQSEAILGKKRVKGLLFADGEIADADLIVMAVGIK 244 + K + + + + + +G A + + Sbjct: 183 AFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLT 242 Query: 245 PNVELAQKSGVQTNRGIIVNDYMETNIPGIYAVGECAEHRGIAYGLVAPLYEQGAVLAKR 304 K N R Sbjct: 243 GKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFV 302 Query: 305 LAGVPTDGYPGSVTSTKLKVSGVDVFSAGQFTERPGTRSLRYQDEIEGIYKKLVIQDDQL 364 Sbjct: 303 362 Query: 365 VGAVLFGDTGDGAQLFSMXXXXXXXXXXXXXXXXXVSSDALASPK--GSRLEGMADDEII 422 Sbjct: 363 ---------- 412 Query: 423 CGCNGVSKGAIAEAIQSGGCTSVGQIKACTKASASCGGCKPLVEGLLQLYAGDNVV--TV 480 Sbjct: 413 472 Query: 481 KEGICGCTTLGRDEIVAEIRRMELKTVKEVMNVLEWNTEEGCAKCRPSLNYYLGMLWPEE 540 Sbjct: 473 532 Query: 541 YIDEKESRITNERYHANIQKDGTYSVVPRIYGGVTSPAELMKIAEVAAKFEVPLVKFTGG 600 Sbjct: 533 ------------- 579 Query: 601 QRLDLLGVKKEDLPKMWEELDMP-SGHAYGKTLRTVKTCVGSTFCRFGTQDAMGMGIRLE 659 Sbjct: 580 ----- 634 Query: 660 KAFERLNTPGKVKLAVSGCPRNCAEATIKDFGVVAIDGGWELHIGGNGGVHVRATDLLCV 719 Sbjct: 635 - 693 Query: 720 VKTDDEVIEWASAFLQYYREQAGWNERTAQWVERVGLDSIKEVLEVRENRLALQERIHTA 779 Sbjct: 694 753 Query: 780 LSVTTDP 786 Sbjct: 754 760 >RSp1222 [C] COG1251 NAD(P)H-nitrite reductase Length = 346 Score = 471 bits (1212), Expect = e-132, Method: Compositional matrix adjust. Identities = 0/820 (0%), Positives = 0/820 (0%), Gaps = 26/820 (3%) Query: 7 KLVLVGNGMAGVRAIEHLLKLSPEAYEITIFGSEPHPNYNRIMLSSVLAGGSSMEEIIIN 66 + + + + + + ++ Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVP-PRPETQ 62 Query: 67 DLEWYHSHGIRLYMGHTITSIDTAKRKVYSDKGIVMPYDELILATGSNPFMLPLPGAEKE 126 L + G I +K S + L + L + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 127 GVIAFRDIKDCQIMQETSQSYRKAVVIXXXXXXXXXXXXXXXXXMEVSVVHIHPYIMERQ 186 + + + + Sbjct: 123 TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNET 182 Query: 187 LDEPASVMLRKELEEQGMKFLLNKQS-EAILGKKRVKGLLFADGEIADADLIVMAVGIKP 245 + + + + + + + + G Sbjct: 183 AFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLT 242 Query: 246 NVELAQKSGVQTNR--GIIVNDYMETNIPGIYAVGECAEHRGIAYGLVAPLYEQGAVLAK 303 L Y A R L Sbjct: 243 GKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFV 302 Query: 304 RL--AGVPTDGYPGSVTSTKLKVSGVDVFSAGQFT-ERPGTRSLRYQDEIEGIYKKLVIQ 360 G T + + DV + + Sbjct: 303 ASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVH 362 Query: 361 DDQ--LVGAVLFGDTGDGAQLFSMXXXXXXXXXXXXXXXXXVSSDALASPKGSRLEGMAD 418 Sbjct: 363 -- 420 Query: 419 DEIICGCNGVSKGAIAEAIQSGGCTSVGQIKACTKASASCGGCKPLVEGLLQLYAGDNVV 478 Sbjct: 421 - 479 Query: 479 TVKEGICGCTTLGRDEIVAEIRRMELKTVKEVMNVLEWNTEEGCAKCRPSLNYYLGMLWP 538 Sbjct: 480 --- 536 Query: 539 EEYIDEKES---RITNERYHANIQKDGTYSVVPRIYGGVTSPAELMKIAEVAAKFEVPLV 595 Sbjct: 537 - 595 Query: 596 KFTGGQRLDLLGVKKEDLPKMWEEL---DMPSGHAYGKTLRTVKTCVGSTFCRFGTQDAM 652 Sbjct: 596 655 Query: 653 GMGIRLEKAFERLNTPGKVKLAVSGCPRNCAEATIKDFGVVAIDGGWELHIGGNGGVHVR 712 Sbjct: 656 715 Query: 713 ATDLLCVVKTDDEVIEWASAFLQYYREQAGWNERTAQWVERV--GLDSIKEVLEVRENRL 770 Sbjct: 716 775 Query: 771 A--LQERIHTALSVTTDPWKQIVNEPELRKNFEQLSEMKT 808 Sbjct: 776 815 >RSp1220 [C] COG1251 NAD(P)H-nitrite reductase Length = 346 Score = 375 bits (964), Expect = e-103, Method: Compositional matrix adjust. Identities = 0/378 (0%), Positives = 0/378 (0%) Query: 5 RKKLVLVGNGMAGVRAIEHLLKLSPEAYEITIFGSEPHPNYNRIMLSSVLAGGSSMEEII 64 L + + +A+ + N + + + + Sbjct: 6 IPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLT 65 Query: 65 INDLEWYHSHGIRLYMGHTITSIDTAKRKVYSDKGIVMPYDELILATGSNPFMLPLPGAE 124 + + + + + R D + + + L Sbjct: 66 ETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDY 125 Query: 125 KEGVIAFRDIKDCQIMQETSQSYRKAVVIXXXXXXXXXXXXXXXXXMEVSVVHIHPYIME 184 + + + S+ + + + Sbjct: 126 LDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFG 185 Query: 185 RQLDEPASVMLRKELEEQGMKFLLNKQSEAILGKKRVKGLLFADGEIADADLIVMAVGIK 244 + +G V + G Sbjct: 186 VMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKY 245 Query: 245 PNVELAQKSGVQTNRGIIVNDYMETNIPGIYAVGECAEHRGIAYGLVAPLYEQGAVLAKR 304 N + +T V H + + +A Sbjct: 246 LNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVAST 305 Query: 305 LAGVPTDGYPGSVTSTKLKVSGVDVFSAGQFTERPGTRSLRYQDEIEGIYKKLVIQDDQL 364 Sbjct: 306 365 Query: 365 VGAVLFGDTGDGAQLFSM 382 Sbjct: 366 383 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 58,486,390 Number of extensions: 2525269 Number of successful extensions: 5355 Number of sequences better than 1.0e-05: 102 Number of HSP's gapped: 5247 Number of HSP's successfully gapped: 103 Length of query: 809 Length of database: 38,378,431 Length adjustment: 115 Effective length of query: 694 Effective length of database: 25,115,021 Effective search space: 17429824574 Effective search space used: 17429824574 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 120 (50.8 bits)