BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0336.0002.p (329 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2351 [P] COG0306 Phosphate/sulphate permeases 362 e-100 BS_ykaB [P] COG0306 Phosphate/sulphate permeases 338 9e-93 lin0428 [P] COG0306 Phosphate/sulphate permeases 314 1e-85 SMc02861 [P] COG0306 Phosphate/sulphate permeases 251 1e-66 mlr8370 [P] COG0306 Phosphate/sulphate permeases 245 7e-65 >lin2351 [P] COG0306 Phosphate/sulphate permeases Length = 332 Score = 362 bits (930), Expect = e-100, Method: Compositional matrix adjust. Identities = 183/328 (55%), Positives = 239/328 (72%) Query: 1 METSLFVLGFVIFLALAFDFINGFHDTANAIATSVSTRALRPRTAIIMAASMNFIGALIF 60 ME + ++ ALAFD INGFHDTANAIATSVST+AL+PR AII+AA MNF+GA+ F Sbjct: 1 MEGMFLITLVIVLAALAFDLINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAISF 60 Query: 61 TGVAKKIGGSITDPSLLDNGVDIVIATLIAAIIWNLVTWWLGIPSSSSHXXXXXXXXXXX 120 TGVAK I I +P LD+G +++A L++AI WNL+TW+ GIPSSSSH Sbjct: 61 TGVAKTITKDIVNPFDLDHGELVILAALLSAIAWNLITWYFGIPSSSSHALIGSIAGAAI 120 Query: 121 XXXXTEHIKWHGFIEIVEGLVFSPLIAFVVGYIVMTILKWIFANRSPHTVNKGFRSMQVI 180 I++ GF +I+ GL+ SP++AFVVGY + ++ K N + T N+ FR +QV Sbjct: 121 ASAGFAAIEYSGFTKIIVGLLVSPVLAFVVGYTIYSLFKIFLKNLNLATTNRRFRMIQVG 180 Query: 181 TAALQSFTHGTNDAQKAMGIITFALVTSGRLDTMEVPLWVKIAAATSMALGTSIGGWKII 240 TAALQS+THGTNDAQK+MGIIT AL+ SG T +V LWV+++ A +MA+GTSIGGWKII Sbjct: 181 TAALQSYTHGTNDAQKSMGIITMALIASGFQSTDDVQLWVQVSCAVAMAIGTSIGGWKII 240 Query: 241 KTMGTKIFKIEPINGFAADISAASVIFSATLLHLPVSTTHAITSSILGVGSAKRFSAVKW 300 KT+G KI KI+P+NG AAD+S+ +IF AT +HLPVSTTH I+SSILGVG+A R VKW Sbjct: 241 KTVGGKIMKIKPVNGVAADLSSVIIIFGATFIHLPVSTTHVISSSILGVGTAHRVKGVKW 300 Query: 301 GVAGRIVVTWFITIPISAVLAGLVFKLI 328 A R+++TW IT+PISA +A L+F ++ Sbjct: 301 DTAQRMIITWVITLPISATIAALIFYVL 328 >BS_ykaB [P] COG0306 Phosphate/sulphate permeases Length = 328 Score = 338 bits (866), Expect = 9e-93, Method: Compositional matrix adjust. Identities = 173/294 (58%), Positives = 219/294 (74%), Gaps = 1/294 (0%) Query: 14 LALAFDFINGFHDTANAIATSVSTRALRPRTAIIMAASMNFIGALIFTGVAKKIGGSITD 73 ALAFDFINGFHDTANAIATSVST+AL+PR AII+AA MNF+GA+ FTGVAK I I D Sbjct: 14 CALAFDFINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAMTFTGVAKTITKDIVD 73 Query: 74 PSLLDNGVDIVIATLIAAIIWNLVTWWLGIPSSSSHXXXXXXXXXXXXXXXTEHIKWHGF 133 P L+NG +++A L+AAI WNL+TW+ GIPSSSSH + + GF Sbjct: 74 PYTLENGSVVILAALLAAIAWNLITWYYGIPSSSSHAIIGAIAGAAIAAAGFAALNYKGF 133 Query: 134 IEIVEGLVFSPLIAFVVGYIVMTILKWIFANRSPHTVNKGFRSMQVITAALQSFTHGTND 193 I+I+E L+ SP+IAFV+G+I+ +I+K IF + + NK FR +Q++TAALQS+THGTND Sbjct: 134 IKIIEALILSPIIAFVLGFILYSIVKLIFKDSNLAKTNKQFRRVQIVTAALQSYTHGTND 193 Query: 194 AQKAMGIITFALVTSG-RLDTMEVPLWVKIAAATSMALGTSIGGWKIIKTMGTKIFKIEP 252 AQKAMGIIT AL+T+ ++P WV+ A AT+M LGTSIGGWKIIKT+G KI KI P Sbjct: 194 AQKAMGIITMALITANLHTSANDIPTWVQFACATAMGLGTSIGGWKIIKTVGGKIMKIRP 253 Query: 253 INGFAADISAASVIFSATLLHLPVSTTHAITSSILGVGSAKRFSAVKWGVAGRI 306 +NG +AD++ A++IF AT +HLPVSTTH I+SSILGVG++ R V WG A RI Sbjct: 254 VNGVSADLTGAAIIFGATFIHLPVSTTHVISSSILGVGASHRVKGVNWGTAKRI 307 >lin0428 [P] COG0306 Phosphate/sulphate permeases Length = 335 Score = 314 bits (805), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 165/314 (52%), Positives = 227/314 (72%), Gaps = 2/314 (0%) Query: 14 LALAFDFINGFHDTANAIATSVSTRALRPRTAIIMAASMNFIGALIFTGVAKKIGGSITD 73 + LAFDFINGFHD ANA+ATS+STRAL+PR AI +AA MNF+GA+ FTGVA+ + SI D Sbjct: 14 MGLAFDFINGFHDIANAVATSISTRALKPRVAIGIAAVMNFLGAISFTGVAESLTKSIVD 73 Query: 74 PSLLDNGVDIVIATLIAAIIWNLVTWWLGIPSSSSHXXXXXXXXXXXXXXXTEHI-KWHG 132 P L+NG +V+ LIAA+IWNL+TW +G+PSSSSH + + W G Sbjct: 74 PFSLNNGEFVVLCGLIAAVIWNLMTWLVGMPSSSSHALIGAIAGASIASASSFSVLNWSG 133 Query: 133 FIEIVEGLVFSPLIAFVVGYIVMTILKWIFANRSPHTVNKGFRSMQVITAALQSFTHGTN 192 F I+ L+ SP+I F VGY++ ++ K +F ++ +N+ FR +Q+ TAA+Q+++HGTN Sbjct: 134 FTTIIIALIISPIIGFTVGYLIYSLFKTLFHDKKLGKMNRRFRFIQIGTAAVQAYSHGTN 193 Query: 193 DAQKAMGIITFALVTSGRL-DTMEVPLWVKIAAATSMALGTSIGGWKIIKTMGTKIFKIE 251 DAQK MGIIT ALV SG L ++ +P WV+++ A SMA+G+S+GG++IIKT+GTKI KI Sbjct: 194 DAQKTMGIITLALVASGLLKESAGIPFWVQVSCAASMAIGSSVGGYRIIKTVGTKIMKIT 253 Query: 252 PINGFAADISAASVIFSATLLHLPVSTTHAITSSILGVGSAKRFSAVKWGVAGRIVVTWF 311 P+ G A+D+S+ SVI SATL+HLPVSTT I SSI+GVG+A V W +VVTWF Sbjct: 254 PVTGVASDLSSLSVIMSATLIHLPVSTTQVIDSSIMGVGTANHKKEVNWRTGKNMVVTWF 313 Query: 312 ITIPISAVLAGLVF 325 IT+P++ +LA +V+ Sbjct: 314 ITLPLAGLLAAVVY 327 >SMc02861 [P] COG0306 Phosphate/sulphate permeases Length = 334 Score = 251 bits (641), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 145/317 (45%), Positives = 200/317 (63%), Gaps = 4/317 (1%) Query: 11 VIFLALAFDFINGFHDTANAIATSVSTRALRPRTAIIMAASMNFIGALIF-TGVAKKIGG 69 +I +AL FDF+NG HD AN+IAT VSTR LRP+ A+ AA NFI L F VA+ +G Sbjct: 13 LIAVALFFDFLNGLHDAANSIATIVSTRVLRPQYAVFWAAFFNFIAFLFFGLHVAETLGT 72 Query: 70 SITDPSLLDNGVDIVIATLIAAIIWNLVTWWLGIPSSSSHXXXXXXXXXXXXXXXTEHIK 129 I DP ++ ++ A L+ AI WN+VTW GIPSSSSH I Sbjct: 73 GIIDPGIVTP--QVIFAALMGAITWNIVTWVFGIPSSSSHALIGGLVGAGLAKTGFSSIV 130 Query: 130 WHGFIEIVEGLVFSPLIAFVVGYIVMTILKWIFANRSPHTVNKGFRSMQVITAALQSFTH 189 W G ++ +V SP I FV+ +++ I+ W+F ++P V+ FR +Q ++A+L S H Sbjct: 131 WQGLLKTAGAIVMSPGIGFVLALLLVLIVSWLFVRQTPFAVDSTFRVLQFVSASLYSLGH 190 Query: 190 GTNDAQKAMGIITFALVTSGRLDT-MEVPLWVKIAAATSMALGTSIGGWKIIKTMGTKIF 248 G NDAQK MGII L + G L + VP WV I ++ALGT GGW+I+ TMG+KI Sbjct: 191 GGNDAQKTMGIIAVLLFSQGYLGSEFYVPFWVVITCQAAIALGTLFGGWRIVHTMGSKIT 250 Query: 249 KIEPINGFAADISAASVIFSATLLHLPVSTTHAITSSILGVGSAKRFSAVKWGVAGRIVV 308 K+ P+ GF A+ A +F+AT L +PVSTTH IT +I+GVG+A+R SAV+WG+AG IVV Sbjct: 251 KLNPMQGFCAETGGAITLFAATWLGIPVSTTHTITGAIIGVGAARRVSAVRWGLAGNIVV 310 Query: 309 TWFITIPISAVLAGLVF 325 W IT+P +A+++ L + Sbjct: 311 AWVITMPAAALISALCY 327 >mlr8370 [P] COG0306 Phosphate/sulphate permeases Length = 332 Score = 245 bits (625), Expect = 7e-65, Method: Compositional matrix adjust. Identities = 138/317 (43%), Positives = 201/317 (63%), Gaps = 4/317 (1%) Query: 11 VIFLALAFDFINGFHDTANAIATSVSTRALRPRTAIIMAASMNFIGALIF-TGVAKKIGG 69 ++ +AL FDF+NG HD AN+IAT VSTR LRP+ A++ AA NFI + F VA+ +G Sbjct: 13 LVAVALFFDFLNGLHDAANSIATIVSTRVLRPQYAVLWAAFFNFIAFMFFGLHVAETVGK 72 Query: 70 SITDPSLLDNGVDIVIATLIAAIIWNLVTWWLGIPSSSSHXXXXXXXXXXXXXXXTEHIK 129 I D S++ V + + L+ AI+WN+VTW GIPSSSSH I Sbjct: 73 GIVDVSIVTPAV--IFSALVGAIVWNIVTWIAGIPSSSSHALIGGLVGAGVAKSGFGAIV 130 Query: 130 WHGFIEIVEGLVFSPLIAFVVGYIVMTILKWIFANRSPHTVNKGFRSMQVITAALQSFTH 189 W G + V ++ SP F++ +++ ++ W+F ++P V+ FR MQ +A+L S H Sbjct: 131 WTGLGKTVAAIIVSPTTGFLLALVLILVVSWLFVRQTPFAVDSTFRVMQFFSASLYSLGH 190 Query: 190 GTNDAQKAMGIITFALVTSGRL-DTMEVPLWVKIAAATSMALGTSIGGWKIIKTMGTKIF 248 G NDAQK MGII L + G L + VPLWV + +++ALGT GGW+I+ TMG+KI Sbjct: 191 GGNDAQKTMGIIAVLLYSQGMLGEKFYVPLWVVLTCQSALALGTLFGGWRIVHTMGSKIT 250 Query: 249 KIEPINGFAADISAASVIFSATLLHLPVSTTHAITSSILGVGSAKRFSAVKWGVAGRIVV 308 ++ P+ GF A+ A +F+AT L +PVSTTH IT +I+GVG+A+R SAV+WG+AG IV+ Sbjct: 251 RLNPMQGFCAETGGAITLFAATWLGVPVSTTHTITGAIIGVGAARRVSAVRWGIAGNIVI 310 Query: 309 TWFITIPISAVLAGLVF 325 W +T+P +A ++ L + Sbjct: 311 AWIVTLPAAAAISALTY 327 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.137 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,544,457 Number of extensions: 825924 Number of successful extensions: 2669 Number of sequences better than 1.0e-05: 38 Number of HSP's gapped: 2554 Number of HSP's successfully gapped: 59 Length of query: 329 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 222 Effective length of database: 26,037,693 Effective search space: 5780367846 Effective search space used: 5780367846 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 116 (49.3 bits)