BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0335.0021.p (290 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0728 [QR] COG0500 SAM-dependent methyltransferases 121 2e-27 TM1389 [QR] COG0500 SAM-dependent methyltransferases 75 1e-13 STM0259 [QR] COG0500 SAM-dependent methyltransferases 62 1e-09 Z0233 [QR] COG0500 SAM-dependent methyltransferases 62 1e-09 ECs0206 [QR] COG0500 SAM-dependent methyltransferases 62 1e-09 >CAC0728 [QR] COG0500 SAM-dependent methyltransferases Length = 272 Score = 121 bits (303), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 75/268 (27%), Positives = 136/268 (50%), Gaps = 28/268 (10%) Query: 32 YYDRYNEAARLTTDHARRLEFITTTHFLDTYL--KGDSQILDLGAGAGIYTFYYAQKGHT 89 YY+++ E RLT + + +E++T+ ++ Y+ +++ILD+GAG G Y+ A +G+ Sbjct: 22 YYNKFCEDKRLTRKYGQ-VEYLTSMKYIHEYIGDNANAKILDVGAGTGRYSVQLANEGYD 80 Query: 90 VISTDLTPKHVEIISQRASDEGYANITAAMADATDLSRYPDESFDAVLCLGPLYHLTDIP 149 V + +L ++ ++ + S + A A DLSR+ + +FD L GP+YHL Sbjct: 81 VTAIELVKHNLGVLRSKGS-----TVKAMQGTALDLSRFSENTFDITLVFGPMYHLYTFE 135 Query: 150 DQDTCIRECLRVLRPGGLLAAAY-VNRLFIYPHLVKS-------DPGFLDTRWIQTILEE 201 D+ ++E RV + GG++ AY +N + + K D G LD+++ I E Sbjct: 136 DKVKALKEAKRVTKVGGVILVAYCMNEYSVITYGFKENNIRECVDNGKLDSKF-HVIAEP 194 Query: 202 KQISSTEADCFWTDAFFHTPGEIEALIEAYGTQKLVHAASDGISILLRETLNGLTTEQFN 261 K + + +I L + +++ A+DG + +R LN + + FN Sbjct: 195 KDLYD-----------YVRIEDINRLDKEVNLKRIKLIAADGPANYMRPVLNAMDEDTFN 243 Query: 262 IWVDYHLRTCTEPSILGMSNHGLYLCRK 289 +++DYH TC P +LG S H + + RK Sbjct: 244 LFLDYHFSTCERPELLGASAHTVDILRK 271 >TM1389 [QR] COG0500 SAM-dependent methyltransferases Length = 248 Score = 75.1 bits (183), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 50/170 (29%), Positives = 89/170 (52%), Gaps = 14/170 (8%) Query: 58 FLDTYLKGDSQILDLGAGAGIYTFYYAQKGHTVISTDLTPKHVEIISQRASDEGYANITA 117 FL+ YLK ++LDLG G G ++ + ++G V+ D + + +E+ A ++G N+ Sbjct: 35 FLEEYLKNPCRVLDLGGGTGKWSLFLQERGFEVVLVDPSKEMLEV----AREKGVKNVVE 90 Query: 118 AMADATDLSRYPDESFDAVLCLGPLYHLTDIPDQDTCIRECLRVLRPGGLLAAAYVNRLF 177 A A+ DL +P +F+AVL LG + L+ + ++D E RVL P GLL A N Sbjct: 91 AKAE--DLP-FPSGAFEAVLALGDV--LSYVENKDKAFSEIRRVLVPDGLLIATVDNFYT 145 Query: 178 IYPHLVKSDPGFLDTRWIQTILEEKQISSTEADCFWTDAFFHTPGEIEAL 227 +++ D TR+++T Q +S F +++ P ++++L Sbjct: 146 FLQQMIEKDAWDQITRFLKT-----QTTSVGTTLFSFNSYAFKPEDLDSL 190 >STM0259 [QR] COG0500 SAM-dependent methyltransferases Length = 256 Score = 62.0 bits (149), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 34/102 (33%), Positives = 57/102 (55%), Gaps = 4/102 (3%) Query: 67 SQILDLGAGAGIYTFYYAQKGHTVISTDLTPKHVEIISQRASDEGYANITAAMADATDLS 126 + +LD+G GAG +F AQ ++V++ DL+ +E+++ A + +NIT A L Sbjct: 46 ASVLDMGCGAGHASFVAAQHANSVVAYDLSASMLEVVAGAAEERHLSNITLRQGYAEKLP 105 Query: 127 RYPDESFDAVLCLGPLYHLTDIPDQDTCIRECLRVLRPGGLL 168 + D SF+ V+ +H D+ +RE RVL+PGG+L Sbjct: 106 -FEDASFEVVISRYSAHHWHDV---GQALREVNRVLKPGGVL 143 >Z0233 [QR] COG0500 SAM-dependent methyltransferases Length = 256 Score = 62.0 bits (149), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 40/133 (30%), Positives = 66/133 (49%), Gaps = 14/133 (10%) Query: 66 DSQILDLGAGAGIYTFYYAQKGHTVISTDLTPKHVEIISQRASDEGYANITAAMADATDL 125 D+ +LD+G GAG +F AQ TV++ DL+ + +++++Q A NIT A L Sbjct: 45 DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAESL 104 Query: 126 SRYPDESFDAVLCLGPLYHLTDIPDQDTCIRECLRVLRPGGLLAAAYVNRLFIYPHLVKS 185 + D +FD V+ +H D+ +RE R+L+PGG RL + + Sbjct: 105 P-FADNAFDIVISRYSAHHWHDV---GAALREVNRILKPGG--------RLIVMDVMSPG 152 Query: 186 DPGFLDTRWIQTI 198 P + W+QT+ Sbjct: 153 HP--VRDIWLQTV 163 >ECs0206 [QR] COG0500 SAM-dependent methyltransferases Length = 256 Score = 62.0 bits (149), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 40/133 (30%), Positives = 66/133 (49%), Gaps = 14/133 (10%) Query: 66 DSQILDLGAGAGIYTFYYAQKGHTVISTDLTPKHVEIISQRASDEGYANITAAMADATDL 125 D+ +LD+G GAG +F AQ TV++ DL+ + +++++Q A NIT A L Sbjct: 45 DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAESL 104 Query: 126 SRYPDESFDAVLCLGPLYHLTDIPDQDTCIRECLRVLRPGGLLAAAYVNRLFIYPHLVKS 185 + D +FD V+ +H D+ +RE R+L+PGG RL + + Sbjct: 105 P-FADNAFDIVISRYSAHHWHDV---GAALREVNRILKPGG--------RLIVMDVMSPG 152 Query: 186 DPGFLDTRWIQTI 198 P + W+QT+ Sbjct: 153 HP--VRDIWLQTV 163 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.137 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,964,283 Number of extensions: 867851 Number of successful extensions: 2274 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 2306 Number of HSP's successfully gapped: 20 Length of query: 290 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 185 Effective length of database: 26,268,361 Effective search space: 4859646785 Effective search space used: 4859646785 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)