BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0335.0019.p (445 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO0995 [G] COG2211 Na+/melibiose symporter and related transpor... 168 1e-41 CAC0694 [G] COG2211 Na+/melibiose symporter and related transpor... 154 2e-37 BS_ydjD [G] COG2211 Na+/melibiose symporter and related transpor... 146 7e-35 STM0149 [G] COG2211 Na+/melibiose symporter and related transpor... 138 2e-32 ZyicJ [G] COG2211 Na+/melibiose symporter and related transporters 124 3e-28 >YPO0995 [G] COG2211 Na+/melibiose symporter and related transporters Length = 477 Score = 168 bits (426), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 123/444 (27%), Positives = 199/444 (44%), Gaps = 13/444 (2%) Query: 4 KVSLAEKMVFSGGLLGQNMLYSFMSMYILFFYTDLLGISTSTASAILVTASIVDACLDPV 63 K+ + E + G +GQ + + +ILFFYTD++GIS AS I + A + DA DP+ Sbjct: 32 KIPMKELASYFGYGMGQCFSFGLVGTFILFFYTDIMGISPVAASMIFLIARVWDAIHDPL 91 Query: 64 MGMITDKTRSRWGKFRPYLLFAPVLIAAATVLCFMDFDTSSALTVTIAAFSYLLWGMLYT 123 + + D R GKFRPYLLF P LI TV F + + S A +Y+LWG LY Sbjct: 92 IAGVMDTINMRRGKFRPYLLFTPFLIFLVTVAAFYNIEASLMTKTIYAGVTYILWGTLYA 151 Query: 124 VCDTPLWALSSVISKDPAERTLVVTLGKXXXXXXXXXXXXXXXQLLLAFGGERSTKXXXX 183 + D P W++S+V++ +P ER T + F E S Sbjct: 152 LSDIPFWSMSTVMTDEPQERAKTATCAMLGVNAGIGATMILFPYISGLF-AENSADRGYF 210 Query: 184 XXXXXXXXXXXXXFLTGMLS-RERV-VPSTERISFRNNLQTVYKNKPXXXXXXXXXXXXX 241 L G + +ERV V TE+++ + V++NKP Sbjct: 211 AGVVILMVLGVILMLNGFFNTKERVNVTVTEKVTLKQTFIVVWQNKP-LFFILSAFFMNV 269 Query: 242 VTGIRQSIQLYYVVYVWGDASYGTQVGISLVAGMLLGMIATPRLLGYFSKKSVFIGSCIL 301 + I + +++ Y GDA + +G+ + L + TP L +F K+ +FI C+L Sbjct: 270 FSNIVNTFYIFFFTYNMGDAELVSVIGL-ITFTCALACLGTPFLTRHFKKRDLFITLCVL 328 Query: 302 GSLSCLVPYLFGDQNXXXXXXXXXXXXXXXXXXXIVSTSMLLDTIDYSEWKLGFRGEGIV 361 ++ + + G N + ++M+ DT++YS + G R I Sbjct: 329 EIIARVGFWFTGYNNVVSVMVWLTVITAIFMMTNPLISAMIADTVEYSYYHTGKRCAAIT 388 Query: 362 FSTNTFITKFSGAVSRLIIGASLGVLNYVENQPVTPGLRHGLSLVMFLLPALCFLAAIIP 421 FS TF+ K S AV+ + G L +L Y+ N + +GL + LLPA + A++ Sbjct: 389 FSGQTFVGKLSVAVAGGVSGLILSILGYMPNVAQSTWTLNGLFFCISLLPA---VGAVVR 445 Query: 422 ILFYTISDKQRTQILMDIHSLTKE 445 IL ++ + D H++ +E Sbjct: 446 ILI-----MRKYKFTEDEHAILRE 464 >CAC0694 [G] COG2211 Na+/melibiose symporter and related transporters Length = 449 Score = 154 bits (389), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 119/448 (26%), Positives = 206/448 (45%), Gaps = 17/448 (3%) Query: 4 KVSLAEKMVFSGGLLGQNMLYSFMSMYILFFYTDLLGISTSTASAILVTASIVDACLDPV 63 K+SL EK+ + G G ++ Y+L FYTD++GI+ A I I DA +DP+ Sbjct: 3 KLSLKEKISYGLGDFGNGFMFDLGQSYLLKFYTDVVGIAAGAAGGIFFFTKIFDAFMDPI 62 Query: 64 MGMITDKTR-SRWGKFRPYLLFAPVLIAAATVLCFMDFDTSSALTVTIAAFSYLLWGMLY 122 G I D + + GKF+P + FA +++A TV+ F + ++ + A +Y++WG+ Y Sbjct: 63 AGTIIDSRKPGKNGKFKPIMFFASIVLAILTVITFTNPGKTATSKLLFAYATYMIWGLGY 122 Query: 123 TVCDTPLWALSSVISKDPAERTLVVTLGKXXXXXXXXXXXXXXXQLLLAFGGERSTKXXX 182 + + P +L SVI++D ERT + T + L+L F Sbjct: 123 SFTNVPYGSLGSVITQDVQERTSLATFRQIGSSGALLITSVIFMPLVLVF--HNPAIGYP 180 Query: 183 XXXXXXXXXXXXXXFLTGMLSRERVVPS----TERISFRNNLQTVYKNKPXXXXXXXXXX 238 ++T +RE V P+ E+I+ ++ T++ N+ Sbjct: 181 VVAGIMGLIGILSFYMTYKNTREVVAPAENVKKEKITPKSIAVTIFTNRALLTLILMTIF 240 Query: 239 XXXVTGIRQSIQLYYVVYVWGDASYGTQVGISLVAGMLLGMIATPRLLGYFSKKSV---- 294 IR S+ +YY Y G+ + + + +LG+ P+L+G F KK Sbjct: 241 SISAYNIRSSLIVYYCQYNLGNVTLLPYINFFTIGCAVLGVSFMPKLVGRFGKKRTAIIG 300 Query: 295 FIGSCILGSLSCLVPYLFGDQNXXXXXXXXXXXXXXXXXXXIVSTSMLLDTIDYSEWKLG 354 F+ S I S++ L+P N ++ + + D+IDY EW+ G Sbjct: 301 FLISVIADSINFLLP-----GNIYTFTILLAIGFIGISIPNGITWAFVSDSIDYGEWRTG 355 Query: 355 FRGEGIVFSTNTFITKFSGAVSRLIIGASLGVLNYVENQPVTPGLRHGLSLVMFLLPALC 414 R EGI +S F K + +++ L+ G LG + YV N+ + G+ ++ PA+ Sbjct: 356 TRREGITYSVFNFARKLAQSIAGLLSGWGLGFVGYVANKKQSAHALFGIKALLMAYPAVA 415 Query: 415 FL-AAIIPILFYTISDKQRTQILMDIHS 441 L AA+I L Y +SDK+ T+I+ ++ + Sbjct: 416 LLVAALIIGLLYNLSDKKFTEIIEELDA 443 >BS_ydjD [G] COG2211 Na+/melibiose symporter and related transporters Length = 463 Score = 146 bits (368), Expect = 7e-35, Method: Compositional matrix adjust. Identities = 107/441 (24%), Positives = 187/441 (42%), Gaps = 1/441 (0%) Query: 2 TTKVSLAEKMVFSGGLLGQNMLYSFMSMYILFFYTDLLGISTSTASAILVTASIVDACLD 61 +T +S E++ + G + N+++ F++ Y+LF+YTD+ GI + + + + ++DA D Sbjct: 19 STGLSWFERIGYGFGDMSYNIIFQFVNAYLLFYYTDVGGIQPAVIATLFLVVRVLDAIFD 78 Query: 62 PVMGMITDKTRSRWGKFRPYLLFAPVLIAAATVLCFMDFDTSSALTVTIAAFSYLLWGML 121 P+MG+I DKT +RWGK RPYLL+ A T LCF + A +Y+L GM Sbjct: 79 PIMGLILDKTNTRWGKARPYLLWVAFPFALFTFLCFTTPHFGETGNMVYAYVTYILLGMS 138 Query: 122 YTVCDTPLWALSSVISKDPAERTLVVTLGKXXXXXXXXXXXXXXXQLLLAFGGERSTKXX 181 +++ P+ +L+ ++ ERT++ T L A GGE Sbjct: 139 FSMQTIPVNSLTGRMTNSVEERTVLTTTRMILVYIGILLSISCATPLATAIGGEDQAFGF 198 Query: 182 XXXXXXXXXXXXXXXFLTGMLSRERVVP-STERISFRNNLQTVYKNKPXXXXXXXXXXXX 240 + RER+ P ++ + L ++KNKP Sbjct: 199 QMTALIYAAVSIVLNLFSFFTVRERIQPKKRKKQGIKKTLSVLFKNKPLLMLISSFLAFA 258 Query: 241 XVTGIRQSIQLYYVVYVWGDASYGTQVGISLVAGMLLGMIATPRLLGYFSKKSVFIGSCI 300 I+ S +YY Y + + L+ + P + +K V I + Sbjct: 259 IGFNIKLSTMVYYFTYNVNHKEFVFMGTVLFFGAALISNLFIPFFSEKWGRKQVMIITAA 318 Query: 301 LGSLSCLVPYLFGDQNXXXXXXXXXXXXXXXXXXXIVSTSMLLDTIDYSEWKLGFRGEGI 360 L +S + + ++ M+ D +DY+EWK G R +G+ Sbjct: 319 LSLISYAGLHFTPYSSIPLIFIWLFASGFFTTPLNTLAWGMVADCVDYAEWKTGIRADGV 378 Query: 361 VFSTNTFITKFSGAVSRLIIGASLGVLNYVENQPVTPGLRHGLSLVMFLLPALCFLAAII 420 V S+ +FI K A++ LG+ YV N T + + + L+P L +II Sbjct: 379 VISSMSFINKLGVALAGSFSAIYLGIAGYVANTDQTVASLNAIKNMNALIPGFFILLSII 438 Query: 421 PILFYTISDKQRTQILMDIHS 441 I FY +++K+ I+ ++ Sbjct: 439 LIAFYPLTEKRYKHIISELEQ 459 >STM0149 [G] COG2211 Na+/melibiose symporter and related transporters Length = 468 Score = 138 bits (347), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 104/389 (26%), Positives = 167/389 (42%), Gaps = 4/389 (1%) Query: 4 KVSLAEKMVFSGGLLGQNMLYSFMSMYILFFYTDLLGISTSTASAILVTASIVDACLDPV 63 ++S+ EK+ + G G N+++ + +++ +FYTD+ G++ + +L++ ++DA DPV Sbjct: 5 RLSVREKIGYGMGDAGCNIIFGAIMLFVNYFYTDIFGLAPALVGVLLLSVRVIDAVTDPV 64 Query: 64 MGMITDKTRSRWGKFRPYLLFAPVLIAAATVLCFMDFDTSSALTVTIAAFSYLLWGMLYT 123 MG + D+T+S++G+FRP+LL+ A +VL F D V A +Y L + YT Sbjct: 65 MGALADRTQSKYGRFRPWLLWIAFPYALFSVLMFTTPDWGYNSKVVYAFVTYFLLSVTYT 124 Query: 124 VCDTPLWALSSVISKDPAERTLVVTLGKXXXXXXXXXXXXXXXQLLLAFGGERSTKXXXX 183 + P +L VI+ DP ER ++ FGG K Sbjct: 125 AINIPYCSLGGVITNDPKERVACQAYRFVLVGIATLLLSLTLLPMVDWFGGGDKAKGYQL 184 Query: 184 XXXXXXXXXXXXXFLTGMLSRERVVPSTE-RISFRNNLQTVYKNKPXXXXXXXXXXXXXV 242 RERV P+ +N+ + V+KN Sbjct: 185 AMTVLAIIGMGMFLFCFASVRERVRPAVPTHDDMKNDFKDVWKNDQWVRILLLTLCNVCP 244 Query: 243 TGIRQSIQLYYVVYVWGDASYGTQVGISL-VAGMLLGMIATPRLLGYFSKKSVFIGSCI- 300 IR + +YYV +V G +++ + ISL V GM++G + L + K VF + I Sbjct: 245 GFIRMAATMYYVTWVMGQSTHFATLFISLGVVGMMIGSMLAKVLTDRWCKLKVFFWTNIA 304 Query: 301 LGSLSCLVPYLFGDQNXXXXXXXXXXXXXXXXXXXIVSTSMLLDTIDYSEWKLGFRGEGI 360 L SC Y F + + S++ D DY EWK G R GI Sbjct: 305 LAIFSCAF-YFFDPKATVMIVALYFLLNILHQIPSPLHWSLMADVDDYGEWKTGKRITGI 363 Query: 361 VFSTNTFITKFSGAVSRLIIGASLGVLNY 389 FS N F K A++ ++G L Y Sbjct: 364 SFSGNLFFLKLGLAIAGAMVGFLLSWYGY 392 >ZyicJ [G] COG2211 Na+/melibiose symporter and related transporters Length = 479 Score = 124 bits (311), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 104/434 (23%), Positives = 178/434 (41%), Gaps = 2/434 (0%) Query: 5 VSLAEKMVFSGGLLGQNMLYSFMSMYILFFYTDLLGISTSTASAILVTASIVDACLDPVM 64 +S+ EK+ + G ++++ + +Y++FFYTD+ GI + + A +DA DP M Sbjct: 25 LSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPAGFVGTMFLVARALDAISDPCM 84 Query: 65 GMITDKTRSRWGKFRPYLLFAPVLIAAATVLCFMDFDTSSALTVTIAAFSYLLWGMLYTV 124 G++ D+TRSRWGKFRP++LF + VL + D S + AA +Y L +LYTV Sbjct: 85 GLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPDLSMNGKMIYAAITYTLLTLLYTV 144 Query: 125 CDTPLWALSSVISKDPAERTLVVTLGKXXXXXXXXXXXXXXXQLLLAFGGERSTKXXXXX 184 + P AL VI+ DP +R + + L+ GG+ Sbjct: 145 VNIPYCALGGVITNDPTQRISLQSWRFVLATAGGMLSTVLMMPLVNLIGGDNKPLGFQGG 204 Query: 185 XXXXXXXXXXXXFLTGMLSRERVVPSTERISFRNNLQTVYKNKPXXXXXXXXXXXXXVTG 244 ++ERV S R +L+ +++N Sbjct: 205 IAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMREDLRDIWQNDQWRIVGLLTIFNILAVC 264 Query: 245 IRQSIQLYYVVYVWGDASYGTQVGISLVAGMLLGMIATPRLLGYFSKKSVFIGSCILGSL 304 +R +YYV ++ G + G L+G L + K ++F + L ++ Sbjct: 265 VRGGAMMYYVTWILGTPEVFVAFLTTYCVGNLIGSALAKPLTDWKCKVTIFWWTNALLAV 324 Query: 305 SCLVPYLFGDQNXXXXXXXXXXXXXXXXXXXIVSTSMLLDTIDYSEWKLGFRGEGIVFST 364 L + Q + M+ DT+DY EW G R GI F+ Sbjct: 325 ISLAMFFVPMQASITMFVFIFVIGVLHQLVTPIQWVMMSDTVDYGEWCNGKRLTGISFAG 384 Query: 365 NTFITKFSGAVSRLIIGASLGVLNYVENQPVTPGLRHGLSLVMF-LLPALCF-LAAIIPI 422 F+ K A +IG L Y + + + +F ++PA+C+ L+AII Sbjct: 385 TLFVLKLGLAFGGALIGWMLAYGGYDAAEKAQNSATISIIIALFTIVPAICYLLSAIIAK 444 Query: 423 LFYTISDKQRTQIL 436 +Y+++ ++ Sbjct: 445 RYYSLTTHNLKTVM 458 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.139 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,881,505 Number of extensions: 866188 Number of successful extensions: 2224 Number of sequences better than 1.0e-05: 42 Number of HSP's gapped: 2160 Number of HSP's successfully gapped: 69 Length of query: 445 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 335 Effective length of database: 25,691,691 Effective search space: 8606716485 Effective search space used: 8606716485 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)