BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0335.0009.p (416 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA1161 [GEPR] COG0477 Permeases of the major facilitator superfa... 331 1e-90 NMA1161 [P] COG1629 Outer membrane receptor proteins, mostly Fe ... 331 1e-90 all2673 [GEPR] COG0477 Permeases of the major facilitator superf... 108 2e-23 sll1204 [GEPR] COG0477 Permeases of the major facilitator superf... 77 7e-14 Cgl0123 [GEPR] COG0477 Permeases of the major facilitator superf... 74 7e-13 >MA1161 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 443 Score = 331 bits (849), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 166/367 (45%), Positives = 242/367 (65%), Gaps = 3/367 (0%) Query: 2 GEWISSIGSGLTAFALGVYVFHMTGTATSVAMVTLCAYVPSIVLGPFGGVLADRFDRRLM 61 G++IS IGSGLT F+LG+YV+ TGTA+S + +CA++P +L P+GG+LADR DRRLM Sbjct: 18 GQFISIIGSGLTTFSLGIYVYQQTGTASSYVFILMCAFLPPFLLKPYGGILADRHDRRLM 77 Query: 62 MITGDLGSAVGLVFILVLMLEGEPSLGQICLGVALSSVFVSLLEPAYKATITDLLSVEQF 121 MI GDLGS +GL+FI +ML+G L QI LG+A+SSVF + EPAYKA ITDLL Q+ Sbjct: 78 MILGDLGSTLGLLFIFAMMLKGNIELWQIYLGIAISSVFSAFQEPAYKALITDLLPENQY 137 Query: 122 AKASGLVQLAASSKYLLSPVLAGSLLAXXXXXXXXXXXXXXXXXXXXXXXXXKRSMNTPK 181 AKASGL+QLA+S++YL+SP LAG +L ++ ++ + Sbjct: 138 AKASGLMQLASSAQYLISPFLAGIILTIMDIKFVFLIDVTTFLIASSIVIWIRKILSRTQ 197 Query: 182 SAAVTGVPLQLFKDLREGWKALASVQGVLQLIFVLSMVTFCAGFLQTLFTPLLLAFTDTR 241 +T DL+EG + + +G++ L+ +++ F G LQ+LF P++L T + Sbjct: 198 ---ITKQEQNNMADLKEGIQEFSKNRGIVNLVITTTLILFFVGLLQSLFIPMMLNLTTVK 254 Query: 242 TLGMIESISAVGMLAGSLVIGIFSLKGNIVRLLAVSLGVAGLCFALVGMSTNTALITSAG 301 +G I+SI A G+L GSL IG++ K V+ L++SL V+G+ FA +G+STN IT AG Sbjct: 255 AVGTIQSICASGILVGSLFIGVYGSKNKYVKTLSISLFVSGIFFANLGLSTNVIFITLAG 314 Query: 302 FLFFAALPFVNTSADVMIRSNIPGEVQGRAWGIIGVLSQFGYVAAYACSGVLSDQVFNPL 361 F+FF+ LPF+NT+ +V+IR NI QGR W II +++ G + A+A +G L+D++FNPL Sbjct: 315 FMFFSTLPFINTAIEVLIRKNIENSKQGRIWSIISMVTYLGSIIAFAVAGFLADKIFNPL 374 Query: 362 LQEGGML 368 L+ G+L Sbjct: 375 LESDGLL 381 >NMA1161 [P] COG1629 Outer membrane receptor proteins, mostly Fe transport Length = 443 Score = 331 bits (849), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 166/367 (45%), Positives = 242/367 (65%), Gaps = 3/367 (0%) Query: 2 GEWISSIGSGLTAFALGVYVFHMTGTATSVAMVTLCAYVPSIVLGPFGGVLADRFDRRLM 61 G++IS IGSGLT F+LG+YV+ TGTA+S + +CA++P +L P+GG+LADR DRRLM Sbjct: 18 GQFISIIGSGLTTFSLGIYVYQQTGTASSYVFILMCAFLPPFLLKPYGGILADRHDRRLM 77 Query: 62 MITGDLGSAVGLVFILVLMLEGEPSLGQICLGVALSSVFVSLLEPAYKATITDLLSVEQF 121 MI GDLGS +GL+FI +ML+G L QI LG+A+SSVF + EPAYKA ITDLL Q+ Sbjct: 78 MILGDLGSTLGLLFIFAMMLKGNIELWQIYLGIAISSVFSAFQEPAYKALITDLLPENQY 137 Query: 122 AKASGLVQLAASSKYLLSPVLAGSLLAXXXXXXXXXXXXXXXXXXXXXXXXXKRSMNTPK 181 AKASGL+QLA+S++YL+SP LAG +L ++ ++ + Sbjct: 138 AKASGLMQLASSAQYLISPFLAGIILTIMDIKFVFLIDVTTFLIASSIVIWIRKILSRTQ 197 Query: 182 SAAVTGVPLQLFKDLREGWKALASVQGVLQLIFVLSMVTFCAGFLQTLFTPLLLAFTDTR 241 +T DL+EG + + +G++ L+ +++ F G LQ+LF P++L T + Sbjct: 198 ---ITKQEQNNMADLKEGIQEFSKNRGIVNLVITTTLILFFVGLLQSLFIPMMLNLTTVK 254 Query: 242 TLGMIESISAVGMLAGSLVIGIFSLKGNIVRLLAVSLGVAGLCFALVGMSTNTALITSAG 301 +G I+SI A G+L GSL IG++ K V+ L++SL V+G+ FA +G+STN IT AG Sbjct: 255 AVGTIQSICASGILVGSLFIGVYGSKNKYVKTLSISLFVSGIFFANLGLSTNVIFITLAG 314 Query: 302 FLFFAALPFVNTSADVMIRSNIPGEVQGRAWGIIGVLSQFGYVAAYACSGVLSDQVFNPL 361 F+FF+ LPF+NT+ +V+IR NI QGR W II +++ G + A+A +G L+D++FNPL Sbjct: 315 FMFFSTLPFINTAIEVLIRKNIENSKQGRIWSIISMVTYLGSIIAFAVAGFLADKIFNPL 374 Query: 362 LQEGGML 368 L+ G+L Sbjct: 375 LESDGLL 381 >all2673 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 437 Score = 108 bits (270), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 102/380 (26%), Positives = 182/380 (47%), Gaps = 21/380 (5%) Query: 1 MGEWISSIGSGLTAFALGVYVFHMTGTATSVAMVTLCAYVPSIVLGPFGGVLADRFDRRL 60 +G+ IS+IGS +T FAL ++V+ TG+AT++A+V A +P I++ F G+ DRF+RR Sbjct: 9 LGQIISTIGSFMTVFALTIWVWDKTGSATALALVGFFAQMPKILITTFAGIAVDRFNRRF 68 Query: 61 MMITGDLGSAVGLVFILVLMLEGEPSLGQICLGVALSSVFVSLLEPAYKATITDLLSVEQ 120 +MI ++ + + + I +L L + + + + V L F L AY A+I+ L+ EQ Sbjct: 69 LMILAEVVAVLCTLAIAILYLNQQLQVWHLYIIVMLYGGFGQLQVLAYSASISLLVPKEQ 128 Query: 121 FAKASGLVQLAASSKYLLSPVLAGSLLAXXXXXXXXXXXXXXXXXXXXXXXXXK--RSMN 178 F +A L S + SP LAG + K + Sbjct: 129 FTRAESLGSAVNYSAAIFSPALAGFIYPRLGLQAIFWIDLTTFAAAFITLLIVKIPQPAV 188 Query: 179 TPKSAAVTGVPLQLFKDLREGWKALASVQGVLQLIFVLSMVTFCAGFLQTLFTPLLLAFT 238 P T L++ L G++ + + + + ++ + L+TP++LA T Sbjct: 189 EPNHRDNTE---SLWQKLTFGFRYIWANPSLTAMAIAFTLFAIPNDISKVLYTPMILART 245 Query: 239 --DTRTLGMIESISAVGMLAGSLVIGIFSLKGNIVRLLAVSLGV--AGLCFALVGMSTNT 294 ++ LG + + + +G + G++ + I+ G R+ + LG G+ L+G+ Sbjct: 246 GGNSEILGTVTTAAGIGGVIGAIALSIWG--GFHRRIHGMLLGFIGHGIFRTLIGLGQTV 303 Query: 295 ALITSAGFLFFAALPFVNTSADVMIRSNIPGEVQGR---AWGIIGVLSQFGYVAAYA--C 349 ++ A FL +P +S++ + + IP +QGR A +IGV VAA Sbjct: 304 SVWMGAHFLSSTLIPLFYSSSNAIWYATIPPGLQGRVLAADQLIGVT-----VAAITPLI 358 Query: 350 SGVLSDQVFNPLLQEGGMLA 369 +G L+D+VF P ++ G+LA Sbjct: 359 AGFLADRVFEPAMRPTGILA 378 >sll1204 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 427 Score = 76.6 bits (187), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 57/248 (22%), Positives = 115/248 (46%), Gaps = 6/248 (2%) Query: 1 MGEWISSIGSGLTAFALGVYVFHMTGTATSVAMVTLCAYVPSIVLGPFGGVLADRFDRRL 60 +G+ +SSIGSG+T F+L ++++ T +A SVA++ + +P I++ P G+L D F + Sbjct: 9 LGQALSSIGSGMTYFSLTIWIWQKTNSAMSVALILVFYQLPQIIITPLSGILTDYFSHKK 68 Query: 61 MMITGDLGSAVGLVFILVLMLEGEPSLGQICLGVALSSVFVSLLEPAYKATITDLLSVEQ 120 ++I D+GSAV + +L ++ I L ++ F ++ +Y + ++ + Sbjct: 69 LLIVSDIGSAVCTFSVGILAFLQILNVKYIYLIASIIGCFGNIQTLSYITLVPLIVPYQH 128 Query: 121 FAKASGLVQLAASSKYLLSPVLAGSLLAX------XXXXXXXXXXXXXXXXXXXXXXXXK 174 A+AS + + A S +++P LAG L K Sbjct: 129 HARASSMGAITAYSAGIVAPALAGILFPVMGLTGITIIDMTTFMIAAITILILPIAFGIK 188 Query: 175 RSMNTPKSAAVTGVPLQLFKDLREGWKALASVQGVLQLIFVLSMVTFCAGFLQTLFTPLL 234 T ++ V + D+ G K + + + +++ + S+ +F G + ++ P++ Sbjct: 189 SPKITNQTLFVVRLKENFVDDIFFGLKYIYNHPNLSKILIIFSLFSFVEGITEVIYQPMI 248 Query: 235 LAFTDTRT 242 LA T T Sbjct: 249 LAKTGGNT 256 >Cgl0123 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 424 Score = 73.6 bits (179), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 40/140 (28%), Positives = 78/140 (55%) Query: 8 IGSGLTAFALGVYVFHMTGTATSVAMVTLCAYVPSIVLGPFGGVLADRFDRRLMMITGDL 67 IG+ LT A+ V ++ MTG++ V + L +P ++ G +GG +AD FD+R+++I + Sbjct: 30 IGAQLTVVAVPVQIYQMTGSSGYVGLTGLFGLIPLVIFGLYGGSIADAFDKRIVLICTTI 89 Query: 68 GSAVGLVFILVLMLEGEPSLGQICLGVALSSVFVSLLEPAYKATITDLLSVEQFAKASGL 127 G V VL + G ++ + + +L F ++ +P A + +L ++Q A A+ L Sbjct: 90 GMCVTTAGFWVLTILGNENIWLLLINFSLQQAFFAVNQPTRTAILRSILPIDQLASATSL 149 Query: 128 VQLAASSKYLLSPVLAGSLL 147 L + ++ P++AG+L+ Sbjct: 150 NMLLMQTGAIVGPLIAGALI 169 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.138 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,207,341 Number of extensions: 858483 Number of successful extensions: 3237 Number of sequences better than 1.0e-05: 19 Number of HSP's gapped: 3247 Number of HSP's successfully gapped: 21 Length of query: 416 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 307 Effective length of database: 25,807,025 Effective search space: 7922756675 Effective search space used: 7922756675 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)