BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0334.0008.p (496 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value L0023 [G] COG2211 Na+/melibiose symporter and related transporters 335 1e-91 YPO1226 [G] COG2211 Na+/melibiose symporter and related transpor... 308 2e-83 L113994 [G] COG2211 Na+/melibiose symporter and related transpor... 223 8e-58 CAC0357 [G] COG2211 Na+/melibiose symporter and related transpor... 211 3e-54 BS_ynaJ [G] COG2211 Na+/melibiose symporter and related transpor... 83 1e-15 >L0023 [G] COG2211 Na+/melibiose symporter and related transporters Length = 508 Score = 335 bits (858), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 192/495 (38%), Positives = 287/495 (57%), Gaps = 30/495 (6%) Query: 14 YGLTDLMGGGAFTIIGAWLMFYYTTFVGMSPXXXXXXXXXXXXXXXXXSLFMGSITDHFY 73 YGLTDLMGGG I+ ++F + G+SP SLF G+ITD FY Sbjct: 24 YGLTDLMGGGWNNIVSG-VIFVFVLSQGISPLFTGAVLGIGRIIDAVWSLFFGAITDRFY 82 Query: 74 KTKLGKKFGRRRFFLLVGSPLM-IVYALLWIDGMSYAFYLVCYLMFEIIAALVLIPWETL 132 +TKLG+K+GRR FF+ +G L I++ + WI S+ +YL Y+ E+ +++LIPWETL Sbjct: 83 RTKLGQKYGRRHFFIALGGILFAILFPMFWISTSSWQYYLWVYVAIEVAISMILIPWETL 142 Query: 133 PSEMTKDFTERTKLSTCRMXXXXLGTFLATFVPGRLIKYFGDDTSYAYFLNGLIFAVLFA 192 P+EMT D+ +RT LS RM GT + F ++K F + +Y Y G+ + V+F Sbjct: 143 PTEMTPDYKKRTTLSGSRMFISATGTAIVFFTLA-VLKSFNNPNAYLY--TGITWTVIFV 199 Query: 193 VCIFITYKVTWERELTPAMLEELEKGQTRQSFADRILALRNMLKDYASTFKIRAFRKHLA 252 + IF +++ +WER LTP L EL+ Q R F+ ++ + DY TFK ++FRKHL Sbjct: 200 IAIFTSWRASWERPLTPEFLAELD-AQPRLGFSG---FMKKTVTDYLGTFKNKSFRKHLL 255 Query: 253 IYLLSFTAKDIFNTVF-VFFCVYCXXXXXXXXXXXXXXXXXGLPITLLAGFAIIKFGPSR 311 +YLLSFT KD ++T+ F + G+ + A ++ GP Sbjct: 256 VYLLSFTGKDFYSTLLPTFIVIGLSGTSANAPWVLQALSAFGILSIIAAVKLMVTRGPKY 315 Query: 312 LYTVSYSSMILCLAGFYMIYLYDPSSKIAIMYVLAVVYQMGRSLLEFTPWNVFPFIPDVD 371 L+T+SY ++I+ + G+++IYL ++ + ++ +++++YQ+GR +LEFTPWNVFPFIPDVD Sbjct: 316 LFTLSYLAIIVAMLGYFVIYLTGINNPMWMLVIVSIIYQLGRGILEFTPWNVFPFIPDVD 375 Query: 372 EAVTQKRREGLFAAVMTFGRKTTVALATFIVGIVLEEGGFVKGQQYQSPEAISVIANTLM 431 T + G++A+VMTF RK+T ALAT+I G LEE GF + ++ ANT + Sbjct: 376 RIFTHGDKAGVYASVMTFFRKSTGALATYIAGWALEEIGF-------NSTTMTTHANTPL 428 Query: 432 FGASALLVLALIFA------------WTFKLNKKTHGVLVKEVDRLKLNGSKSQVDPETQ 479 G ++L IF TFKLN+ H +L E+ RL+ GSK+ V ET+ Sbjct: 429 -GIQHGIMLIFIFVPIGLLAAALIAARTFKLNRANHDILKAELQRLEEGGSKADVTLETR 487 Query: 480 KIVEDLTGYKYEKIW 494 ++VE LTG+ YEK W Sbjct: 488 QVVELLTGHSYEKCW 502 >YPO1226 [G] COG2211 Na+/melibiose symporter and related transporters Length = 498 Score = 308 bits (788), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 156/489 (31%), Positives = 265/489 (54%), Gaps = 14/489 (2%) Query: 12 IGYGLTDLMGGGAFTIIGAWLMFYYTTFVGMSPXXXXXXXXXXXXXXXXXSLFMGSITDH 71 + YG +L+G GA I GAWLM++YTTF G+S + MG ITD+ Sbjct: 1 MAYGSANLLGSGALAISGAWLMYFYTTFCGLSVVEAATIFSIASIIDAISNPVMGYITDN 60 Query: 72 FYKTKLGKKFGRRRFFLLVGSPLMIVYALLWIDGMSYAFYLVCYLMFEIIAALVLIPWET 131 FY T+LG+ FGRRRFF+L+G PL++VY +LW+ G + +YL+ Y +FE+I +++P+ET Sbjct: 61 FYNTRLGRIFGRRRFFILLGIPLVLVYPMLWMSGFGFWYYLLTYALFELIYTSIMVPYET 120 Query: 132 LPSEMTKDFTERTKLSTCRMXXXXLGTFLATFVPGRLIKYFGDDTSYAYFLNGLIFAVLF 191 L +EMT DF +R+KL+ + + FLA F+PG+ I +G D++ + G+ + ++ Sbjct: 121 LATEMTTDFAKRSKLTGSKAIFGKVANFLAAFIPGQFIAIYGKDSATPFLYTGIAYGLIM 180 Query: 192 AVCIFITYKVTWERELTPAMLEELEK-GQTRQSFADRILALRNMLKDYASTFKIRAFRKH 250 + Y +WER + + E GQ AL+ + D ASTF +R FRKH Sbjct: 181 CCAMIWLYSSSWERPASEVVRETTSSLGQ----------ALKKLCVDMASTFHLRIFRKH 230 Query: 251 LAIYLLSFTAKDIFNTVFVFFCVYCXXXXXXXXXXXXXXXXXGLPITLLAGFAI-IKFGP 309 L +YL F A+ +F + F +F ++ I+ I +K G Sbjct: 231 LGMYLFGFGAEWLFASAFTYFIIFGLGQDAALVSQLNSFSSIMQLISTAIFIGICVKMGF 290 Query: 310 SRLYTVSYSSMILCLAGFYMIYL--YDPSSKIAIMYVLAVVYQMGRSLLEFTPWNVFPFI 367 +R + ++ +I+ + + +Y + ++ + +++ + V+ + + + PW V+ F+ Sbjct: 291 ARPFRIALQVVIVSVVAYAALYFTGWSETTTVIVLFCITAVFGLSTGGIYYIPWTVYTFL 350 Query: 368 PDVDEAVTQKRREGLFAAVMTFGRKTTVALATFIVGIVLEEGGFVKGQQYQSPEAISVIA 427 DVDE +T +RREG++A MTF K ++ F +G L GFV GQ Q A+ I Sbjct: 351 ADVDEVLTGRRREGIYAGAMTFAGKMVRSIIVFAMGWTLSRFGFVSGQSSQPEIAVQAIV 410 Query: 428 NTLMFGASALLVLALIFAWTFKLNKKTHGVLVKEVDRLKLNGSKSQVDPETQKIVEDLTG 487 G +L ++A+ + KL++K H +L++E++R+K G+ + + + + E+LTG Sbjct: 411 GVFAIGVISLALVAIYYTTQMKLDRKNHSILLEEIERIKNGGAMADIPAHARAVAEELTG 470 Query: 488 YKYEKIWNQ 496 +KYE+ W Sbjct: 471 WKYEQCWGN 479 >L113994 [G] COG2211 Na+/melibiose symporter and related transporters Length = 581 Score = 223 bits (567), Expect = 8e-58, Method: Compositional matrix adjust. Identities = 155/552 (28%), Positives = 258/552 (46%), Gaps = 71/552 (12%) Query: 11 SIGYGLTDLMGGGAFTIIGAWLMFYYTTFVGMSPXXXXXXXXXXXXXXXXXSLFMGSITD 70 ++G G D+ GG +IG+WL+++ T+F G++P + + I+D Sbjct: 30 ALGIGSWDMFNGGTGGLIGSWLLYFMTSFGGVNPGLAALLISARLVIDMFWAPAVAVISD 89 Query: 71 HFYKTKLGKKFGRRRFFLLVGSPLMIVYALLWI---DGMSYAFYLVCYLMFEIIAALVLI 127 +FY LG K+GRRRFFL+ P I +A +WI G+S+ +YLV +++F+ LVLI Sbjct: 90 NFYHFALGLKYGRRRFFLIFAIPTSIAFAAMWIPMAGGLSWLYYLVSFILFDFCLDLVLI 149 Query: 128 PWETLPSEMTKDFTERTKLSTCRMXXXXLGTFLATFVPGRLIKYFGDD-----TSYAYFL 182 PWE +P+E+T +T+R K+ + RM L L FVP +K + +++A F Sbjct: 150 PWEAIPAEITSTYTQRNKMGSIRMWASGLAQPLIAFVPTLFMKLLPAEHGMQTSAWALFA 209 Query: 183 NGLIFAVLFAVCIFITYKVTWE-----RELTPAMLEELEKGQTRQSFADRILALRNMLKD 237 ++ V+ + Y +W+ +E ML L++ + R R+ L N + + Sbjct: 210 TACVWGVIGIIFTLFVYFSSWDPILISKEKIELMLRHLDEERKRAKTPARLF-LEN-IAN 267 Query: 238 YASTFKIRAFRKHLAIYLLSFTAKDIFNTVFVFFCVYCXXXXXXXXXXXXXXXXXGLPIT 297 T +I+ FRKHL +Y +F D F +FV F P+ Sbjct: 268 LLLTLRIKTFRKHLVLYFSTFGIIDSFTAIFVIFAT-ISFLPTLTLDPAQAALILATPLL 326 Query: 298 LLAG---------FAIIKFGPSRLYTVSYSSMILCLAGFYMIYLYDPSSKIAIMYVLAV- 347 ++ F K GP LY + + IL + ++Y S A + L + Sbjct: 327 IMTIAYTPFGAWLFNHPKIGPRLLYLIGFGIGILACITYGIVYFTRGSLSEAQITGLIIF 386 Query: 348 ---VYQMGRSLLEFTPWNVFPFIPDVDEAVTQKRREGLFAAVMTFGRKTTVALATFIVGI 404 V+ +GR+++ PW VFP IPD+D + +++R G+FA MTF RK T + ++G Sbjct: 387 TTCVFTVGRNIIGGLPWVVFPLIPDIDMIIHKEQRAGVFAGAMTFVRKFTNIVFNVVIGA 446 Query: 405 VLEEGGFVKGQ-------------------------------QYQSPEAISVIANTLMFG 433 V+ G+ + ++Q A IA + F Sbjct: 447 VMGAAGYNQHVSNVADKMSNYVQVHHVTTRQAFHHLVTSSTLEHQIQSAGFGIAMLMTFL 506 Query: 434 ASALLVLALIFAWTFKLNKKTHGVLVKEVDRLK----------LNGSKSQVDPETQKIVE 483 L+++AL A+TFKLN KTH +LV+E+ RL+ + +K D +T++ +E Sbjct: 507 VGGLMLIALWNAFTFKLNSKTHAILVEEISRLQKADSIGGEVAVKNAKESADLKTKQTIE 566 Query: 484 DLTGYKYEK-IW 494 L+G Y+K +W Sbjct: 567 ALSGLNYDKYVW 578 >CAC0357 [G] COG2211 Na+/melibiose symporter and related transporters Length = 513 Score = 211 bits (536), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 142/503 (28%), Positives = 247/503 (49%), Gaps = 17/503 (3%) Query: 3 KKKVTIPVSIGYGLTDLMGGGAFTIIGAWLMFYYTTFVGMSPXXXXXXXXXXXXXXXXXS 62 K+K+ + +GY + +G GA ++ AWL+F+Y++ G++ + Sbjct: 2 KEKIKLSNILGYSSINFLGSGAQGLMSAWLLFFYSSVCGINSVKAASIFAIARLIDAVGN 61 Query: 63 LFMGSITDHFYKTKLGKKFGRRR-FFLLVGSPLMIVYALLWIDGMSYAFYLVCYLMFEII 121 +G I+D F KTKLG++FGRR+ F L + IV+ LLWI G S+ +Y V +++E+ Sbjct: 62 PVLGFISDGFGKTKLGRRFGRRKPFIALGIIGIAIVFPLLWISGKSFIYYFVVNMIYEVS 121 Query: 122 AALVLIPWETLPSEMTKDFTERTKLSTCRMXXXXLGTFLATFVPGRLIKYFGDDTSYAYF 181 LV +P TLP+EMT+D E+ KL + + + F+ G + FG ++ +++ Sbjct: 122 YTLVFVPGTTLPAEMTQDAAEKAKLIGGKQYCGNIANTITGFLTGFIFGSFGKTSTKSFW 181 Query: 182 LNGLIFAVLFAVCIFITYKVTWERELTPAMLEELEKGQTRQSFADRILALRNMLKDYAST 241 GL + ++ + + +ER+ A+ E+ + G + F + + D S Sbjct: 182 YIGLSWGIITTIALLFFIFNVYERDPKTAVYED-DAGSLIEVF-------KKLGIDLVSC 233 Query: 242 FKIRAFRKHLAIYLLSFTAKDIFNTVFVFFCVYCXXXXXXXXXXXXXXXXXGLPITLLAG 301 + +AFR H ++ L K + V FF VY I LLA Sbjct: 234 MRNKAFRLHSVMWFLGSVYKQLAQGVLTFFAVYVLSLTTVAVSNVNGVSSLVSCIALLAY 293 Query: 302 FAII-KFGPSRLYTVSYSSMILCLAGF-YMIYLYDPSSKIAIMYVLAVVYQMGRSLLEFT 359 + K G + + + + + L G+ Y+++ S + + V +++ +G++ +++ Sbjct: 294 ILLAYKIGAPKTFKLGIVIIFVSLLGYVYLVFTGHNSLTLTLFVVFSIINIVGKAAVDYI 353 Query: 360 PWNVFPFIPDVDEAVTQKRREGLFAAVMTFGRKTTVALATFIVGIVLEEGGFV------K 413 P FI D+DEA+T KRREG++ + K ++ + ++GI L GF+ K Sbjct: 354 PTYQMGFIADIDEAITLKRREGVYNGINGLFGKLATSIESMVLGIGLGAFGFISTSAKAK 413 Query: 414 GQQYQSPEAISVIANTLMFGASALLVLALIFAWTFKLNKKTHGVLVKEVDRLKLNGSKSQ 473 +Q AI I+ + LL + I A FKL K+ H +L+ EVDR+K GS ++ Sbjct: 414 HMPHQPHSAIVGISIVTIVFPLVLLTITWIAASRFKLTKENHKLLIDEVDRIKAGGSLNE 473 Query: 474 VDPETQKIVEDLTGYKYEKIWNQ 496 V PE + VE LTG+KYEK + Sbjct: 474 VSPEAKTAVEALTGWKYEKCFGN 496 >BS_ynaJ [G] COG2211 Na+/melibiose symporter and related transporters Length = 85 Score = 82.8 bits (203), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 0/478 (0%), Positives = 0/478 (0%), Gaps = 52/478 (10%) Query: 4 KKVTIPVSIGYGLTDLMGGGAFTIIGAWLMFYYTTFVGMSPXXXXXXXXXXXXXXXXXSL 63 K + L + + + +S + Sbjct: 7 KSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIPLSEWTTSMYVIQSSMIFVYSLV 66 Query: 64 FMGSITDHFYKTKLGKKFGRRRFFLLVGSPLMIVYALL------WIDGMSYAFYLVCYLM 117 F + Sbjct: 67 FTVLLAI----- 121 Query: 118 FEIIAALVLIPWETLPSEMTKDFTERTKLSTCRMXXXXLGTFLATFVPGRLIKYFGD--- 174 Sbjct: 122 181 Query: 175 DTSYAYFLNGLIFAVLFAVCIFITYKVTWERELTPAMLEELEKGQTRQSFADRILALRNM 234 Sbjct: 182 ------------- 228 Query: 235 LKDYASTFKIRAFRKHLAIY-LLSFTAKDIFNTVFVFFCVYCXXXXXXXXXXXXXXXXXG 293 Sbjct: 229 ------------ 276 Query: 294 LPITLLAGFAIIKFGPSRLYT-------VSYSSMILCLAGFYMIYLYDPSSKIAIMYVLA 346 Sbjct: 277 ---- 332 Query: 347 VVYQMGRSLLEFTPWNVFPFIPDVDEAVTQKRREGLFAAVMTFGRKTTVALATFIVGIVL 406 Sbjct: 333 392 Query: 407 EEGGFVKGQQYQSPEAISVIANTLMFGASALLVLALIFAWTFKLNKKTHGVLVKEVDR 464 Sbjct: 393 - 449 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.143 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,300,977 Number of extensions: 1158566 Number of successful extensions: 4288 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 4246 Number of HSP's successfully gapped: 39 Length of query: 496 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 385 Effective length of database: 25,576,357 Effective search space: 9846897445 Effective search space used: 9846897445 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 118 (50.1 bits)