BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0334.0005.p (476 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll0854 [G] COG1653 ABC-type sugar transport system, periplasmic... 145 2e-34 TM0810 [G] COG1653 ABC-type sugar transport system, periplasmic ... 106 7e-23 alr2722 [G] COG1653 ABC-type sugar transport system, periplasmic... 90 1e-17 BMEII0542 [G] COG1653 ABC-type sugar transport system, periplasm... 89 2e-17 all1027 [G] COG1653 ABC-type sugar transport system, periplasmic... 75 3e-13 >mll0854 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 430 Score = 145 bits (365), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 110/438 (25%), Positives = 183/438 (41%), Gaps = 15/438 (3%) Query: 33 SQRAACIFLIVVLGLLLSGCFPKSDNAADTAEHGGKVVLTYWDENAGPSRTPILQELLRR 92 + + + L V GL +S +D+ E V+T P RT L++ + Sbjct: 5 NMKKLTVMLATVAGLAISAGSALADSTLKMVE-----VIT------SPPRTEFLKKQIAE 53 Query: 93 FEQKNPDIQVKYEGMPAGINKAKYDLAVASGDLPDAGGINGEWIADYAAKGVLLPLDAYF 152 FE NP ++V+ +P G K+ V +GD PD + W+ YA L L Y Sbjct: 54 FEAANPGVKVELISLPWGQAFEKFLTMVQAGDTPDVVEMPERWMGLYANNAQLEDLGPYM 113 Query: 153 ADWPERGQIDPSFIDYNRSLTQDGKLYQIPSTYYLDVFWYRSDWFAAAGLPRPQTWEDIF 212 A W + + + ++ + + IP YY++ ++ F AGL P D F Sbjct: 114 AKWDDAKTLGERAKQFGSTV--NNTQFMIPYGYYVNALFWNKKLFKQAGLDGPPATLDEF 171 Query: 213 RSVRVLANTRQGSFGYSLRGGEGSVAQLTSLMYAYSGITGSFRTDGSSTVNDPVHIEFLK 272 + + G +GY LRGG G+ + M G G F DG+ST+N+ ++ L+ Sbjct: 172 VADSKKISAIPGKYGYCLRGGPGAFNGMHMFMNIAQGKGGYFNKDGTSTINEEGSVKGLQ 231 Query: 273 RYLALYKAYTPASDIVS-GYKEVVANFDSGRAAMIQHNLGSYQDHKKALGTGHFDGVLLP 331 +YK D VS G+ E V F SG AM+ + + + F LP Sbjct: 232 MLADMYKDGLAPKDAVSWGFNETVTGFYSGTCAMLNQDPDALLGIADKMSADDFAVAPLP 291 Query: 332 PSINGTRTLFGNANGYGIMRTTRHPDEAWRLIRYLLSEESQIYWNSQVGQIPTNLKAWND 391 +G G+ + ++H D+AW+L+ LLS + + W +VG +P + A D Sbjct: 292 VGPSGKSYPTLGYAGWAMFANSQHKDDAWKLMATLLSPKDNLEWAKEVGVVPIHNGADQD 351 Query: 392 PYFLSHPVLREAIEAFRSPDTAFVRAPYELPDYPSLIRLIE-PEFQQVLAGTMDVEHFLN 450 P+F + E + V P L + + + + FQ+VL G + + Sbjct: 352 PHFKTEQFKGWFTELSDTSKYEMVTPPTHLENLGNFVDQVAIKNFQEVLLGQKTAKDVAD 411 Query: 451 FWAQLMENSRRQHLHSNE 468 WA + ++ L N+ Sbjct: 412 QWAAFLTKEQQDWLAKNK 429 >TM0810 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 420 Score = 106 bits (265), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 93/368 (25%), Positives = 172/368 (46%), Gaps = 38/368 (10%) Query: 40 FLIVVLGLLLSGCFPKSDNAADTAEHGGKVVLTYWDENAGPSRTPILQELLRRFEQKNPD 99 FL++++ +LL+ K + +W + P+ T +Q ++ R+E+ NPD Sbjct: 4 FLVILMVVLLAVL------------ALSKTKIVFWTMSLKPTFTDFIQGIIDRYEELNPD 51 Query: 100 IQVKYEGMPAGINKAKYDLAVASGDLPDAGGINGEWIADYAAKGVLLPLDAYFADWPERG 159 +++ +E +P + + K A +SG+ PD +N +W ++A K VL PL+ PE Sbjct: 52 VEIVWEDVPWDVLQQKLLAAFSSGNPPDVVNLNAQWTIEFAQKKVLFPLNDLL---PEE- 107 Query: 160 QIDPSFIDYNRSLTQDGKLYQIPSTYYLDVFWYRSDWFAAAGL-PR--PQTWEDIFRSVR 216 I+ F + + LT +Y IP +DV +Y + F AGL P+ P+TW++I Sbjct: 108 VINQYFDNMIKGLTWKDGIYGIPWYTAVDVIFYNKEIFEKAGLDPKYPPRTWDEILL-YS 166 Query: 217 VLANTRQGSFGYSLRGGEGSVAQLTSLMYAYSGITGSFRTDGSSTVND-----PVHIEFL 271 VL + G + G ++ Q S ++ + G+ + D ++ + + P + L Sbjct: 167 VLIKEKTGKY-----GALPTIFQDPSAIFNWDGL-NLYTVDENNRIKEVLFDRPEYAHTL 220 Query: 272 KRYLALYKAYTPASDIVSG--YKEVVANFDSGRAAMIQHNLGSYQDHKKALGTGHF---D 326 ++ LYK S+IV G + + +G AM+ + + D K + D Sbjct: 221 NKWATLYKQKYIPSEIVQGGEWTRATELYQAGELAMLITGV-QFADRVKWNAPEIYEKSD 279 Query: 327 GVLLPPSINGTRTLFGNANGYGIMRTTRHPDEAWRLIRYLLSEESQIYWNSQVGQIPTNL 386 +P G R + G + ++R +++P EA + ++ + E+QI + V PT Sbjct: 280 VAPIPAPKPGVR-MSGWYSTLNVVRGSKNPKEAAKFAAFVANLENQIAFCKLVTIFPTLK 338 Query: 387 KAWNDPYF 394 A NDP+F Sbjct: 339 AAVNDPWF 346 >alr2722 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 359 Score = 89.7 bits (221), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 0/376 (0%), Positives = 0/376 (0%), Gaps = 25/376 (6%) Query: 43 VVLGLLLSGCFPKSDNAADTAEHGGKVVLTYWDENAGPSRTPILQELLRRFEQKNPDIQV 102 L NA L D L+ + ++ Sbjct: 21 RELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEG 80 Query: 103 KYEGMPAGI---NKAKYDLAVASGDLPDAGGINGEWIADYAAKGVLLPLDAYFADWPERG 159 + + + AV + + A + + + Sbjct: 81 --FFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAE 138 Query: 160 QIDPSFIDYNRSLTQDGKLYQIPSTYYLDVFWYRSDWFAAAGLPR-PQTWEDIFRSVRVL 218 + + D + A + Sbjct: 139 AFYHRLTQ---CKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASG 195 Query: 219 ANTRQG-----SFGYSLRGGEGSVAQLTSLMYAYSGITGSFRTDGSSTVNDPVHIEFLKR 273 + S + S + S Sbjct: 196 GILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGC-QPVMSLTSSLIAVREHKAGEPVG 254 Query: 274 YLALYK-AYTPASDIVSGYKEVVANFDSGRAAMIQHNLGSYQDHKKALGTGHFDGVLLPP 332 Y + +V+ + + + + Sbjct: 255 YGGTWVSERDTRLGVVAMGYG--DGYPRAAPSG--TPVLVNGREVPI----VGRVAMDMI 306 Query: 333 SIN-GTRTLFGNANGYGIMRTTRHPDEAWRLIRYLLSEESQIYWNSQVGQIPTNLKAWND 391 ++ Sbjct: 307 CVDL 366 Query: 392 PYFLSHPVLREAIEAF 407 Sbjct: 367 382 >BMEII0542 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 398 Score = 88.6 bits (218), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 80/366 (21%), Positives = 157/366 (42%), Gaps = 24/366 (6%) Query: 84 PILQELLRRFEQKNPDIQVKYEGMPAGINKAKYDLAVASGDLPDAGGINGEWIADYAAKG 143 P +E+ + FE +NP I + +E +P + K +++G D I W+ D+ +G Sbjct: 28 PYFEEVKKAFEAENPGITLNFEVVPWDVLLQKLTTDISAGTNADLSIIGTRWLIDFVQQG 87 Query: 144 VLLPLDAYFADWPERGQIDPSFIDYNRSLTQDGKLYQIPSTYYLDVFWYRSDWFAAAGLP 203 + PLD Y D +G+ +F+ + DGK Y +P + +Y D F AG+ Sbjct: 88 IAEPLDGYMND-EFKGRFIETFLSPS---VLDGKTYGLPIAASVRAMYYNKDLFEKAGIK 143 Query: 204 R-PQTWEDIFRSVRVLANTRQGSFGYSLRGGEGSVAQLTSLMYAYSGI----TGSFRTDG 258 P W+++ + ++G+ L+G E + + +Y Y + T DG Sbjct: 144 NPPANWDELKADAAKIKALGGENYGFGLQGKE-----IETDVYYYYAMWSYGTEILNKDG 198 Query: 259 SSTVNDPVHIEFLKRYLALYKAYTPASDIVSGYKEVVAN-FDSGRAAMIQHNLGSYQDHK 317 +S ++ P +E K Y ++ + S +E V N F G+ M+ K Sbjct: 199 TSGLSTPGALEAAKLYKSMIDDGLTQPGVTSYAREDVQNLFKQGKVGMMITAPFLSNQIK 258 Query: 318 KALGTGHFDGVLLPPSINGTRTLFGNANGYGIMRTTRHPDEAWRLIRYLLSEESQIYWNS 377 + + +P G R +G + + + +++ +EAW+++ +L ++ + + Sbjct: 259 EEAPNLKYGVAAIPAGPTGARGTYGVTDSVIMFQNSKNKEEAWKVLDFLFQKDWRAKFTQ 318 Query: 378 QVGQIPTNLKAWNDPYFLSHPVLREAIEAFRS--PDTAFVRAPYELPDYPSLIRLIEPEF 435 G +P N + Y++++ L AF + PD F AP +P + + + Sbjct: 319 NEGFLPVNKEEAKMDYYVNNADLA----AFTALLPDARF--APV-IPGWEEIADITSNAM 371 Query: 436 QQVLAG 441 Q + G Sbjct: 372 QSIYLG 377 >all1027 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 432 Score = 74.7 bits (182), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 84/383 (21%), Positives = 158/383 (41%), Gaps = 28/383 (7%) Query: 37 ACIFLIVVLGLLLSGCFPKSDNAADTAEHGGKVVLTYWDENAGPSRTPILQELLRRFEQK 96 AC L ++ ++S C + N G + +W P T Q L+ FE + Sbjct: 12 ACAMLGLITSWMVS-CSTGNVNTNTQQTASGTANIEFWTMQLQPQFTNYFQSLIANFETQ 70 Query: 97 NPDIQVKYEGMPAGINKAKYDLAVASGDLPDAGGINGEWIADYAAKGVLLPLDAYFADWP 156 NP I+V + +P + K AV++ PD +N ++ + A + L LD P Sbjct: 71 NPGIKVNWVDVPWAAMENKILTAVSAKTPPDVVNLNPDFASQLAGRNAWLDLDTKV---P 127 Query: 157 ERGQIDPSFI-DYNRSLTQDGKLYQIPSTYYLDVFWYRSDWFAAAGLPR-PQTWEDIFRS 214 + + S++ + ++ T +GK + +P + Y +D AG+ + P T+ ++ ++ Sbjct: 128 QEAR--SSYLPNIWKASTLNGKSFGVPWYLTTRLTIYNTDLLKQAGISKPPATYAELAQA 185 Query: 215 VRVLANTRQGSFGYSLR---GGEGSVAQLTSLMYAYSGITGSFRTDGSSTVNDPVHIEFL 271 + + + + G + + + G V Q + G T T+G + N P Sbjct: 186 AQQIKD-KTGKYAFFVTFVPQDSGEVLQ----SFVQMGAT-LVDTEGKAAFNSPQGKAAF 239 Query: 272 KRYLALY-KAYTPASDIVSGYKEVVANFDSGRAAMIQHNLGSYQDHKKALGTGH---FDG 327 + ++ LY K P + G++ + + SG A L S + K + T Sbjct: 240 QYWVDLYKKGLLPKEALTQGHRHAIDLYQSGETAF----LASGPEFLKTITTNAPKVAQA 295 Query: 328 VLLPPSINGTRTLFGNANGYGIM--RTTRHPDEAWRLIRYLLSEESQIYWNSQVGQIPTN 385 + P + G T N I+ R T+ PD A + ++ ++E+Q+ + +P+ Sbjct: 296 SAIAPQLTG-DTGKKNVAVMNIVVPRDTKQPDAAVKFALFVTNDENQLAFAKAANVLPST 354 Query: 386 LKAWNDPYFLSHPVLREAIEAFR 408 KA D YF P +E R Sbjct: 355 TKALADSYFKDIPADASTVEKAR 377 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 37,348,265 Number of extensions: 1644586 Number of successful extensions: 4964 Number of sequences better than 1.0e-05: 39 Number of HSP's gapped: 4944 Number of HSP's successfully gapped: 39 Length of query: 476 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 366 Effective length of database: 25,691,691 Effective search space: 9403158906 Effective search space used: 9403158906 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)