BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0333.0012.p (474 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0518 [G] COG0469 Pyruvate kinase 526 e-149 VC0485 [G] COG0469 Pyruvate kinase 485 e-137 ZpykF [G] COG0469 Pyruvate kinase 476 e-134 pykF [G] COG0469 Pyruvate kinase 476 e-134 ZpykF [G] COG0469 Pyruvate kinase 476 e-134 >CAC0518 [G] COG0469 Pyruvate kinase Length = 473 Score = 526 bits (1356), Expect = e-149, Method: Compositional matrix adjust. Identities = 256/472 (54%), Positives = 345/472 (73%), Gaps = 1/472 (0%) Query: 1 MRKSKIVCTIGPASESLENIKKLILAGMNVARLNFSHGDFEEHGNRIKTIRQASQELGKT 60 M+K+K++ T+GPASE+ E + I AGMN +R NFSHGD EHG RI +++ +L K Sbjct: 1 MQKTKMIFTVGPASETEEIVTAFIKAGMNASRHNFSHGDHAEHGGRIALVKKVRAKLNKP 60 Query: 61 VAILLDTKGPEIRTGKLEVEPIELVQDEYLTLTT-EEILGDQNRISVTYADLPSDVQVGS 119 VAI LDTKGPEIRTG +EL + T+ EEI+GD + S++Y DL DV+ G+ Sbjct: 61 VAICLDTKGPEIRTGDFNPSKLELQKGSKFTIVCGEEIVGDATKCSISYKDLYKDVKPGN 120 Query: 120 TILIDDGLIGLTVVDIQGTEIKTRIVNGGTIKSKKGVNVPGVAISLPGITEKDTNDIIFG 179 TILIDDGL+GLTV I+GT + + N G + S KGVNVP V+I LP +TEKD +D+IFG Sbjct: 121 TILIDDGLVGLTVEAIEGTNVICTVANTGLVGSHKGVNVPNVSIQLPAMTEKDKSDLIFG 180 Query: 180 IEQDIDFIAASFVRKASDVQEIRALLEKHNASHIQIISKIENQQGVDNLDEILAVSDGLM 239 +++ID ++ASF+RK DV IR +L ++ +IQI SKIENQ+GVDN+D I+ VSDG+M Sbjct: 181 CKEEIDMVSASFIRKPEDVLAIRKVLNENGGENIQIFSKIENQEGVDNIDAIIEVSDGIM 240 Query: 240 VARGDLGVEIPAEDVPLAQKLMIQKCNIAGKPVITATQMLDSMQRNPRPTRAEASDVANA 299 VARGD+GVEIP + VPL QK++I+KCN GKPVITATQMLDSM RNPRPTRAEASD+ANA Sbjct: 241 VARGDMGVEIPIQRVPLIQKMIIKKCNAVGKPVITATQMLDSMMRNPRPTRAEASDIANA 300 Query: 300 IFDGTDAIMLSGETAAGKYPVESVLTMSRIAEKAESALNHREIFMKQQIAQETTVTEAIS 359 IFDGTDAIMLSGE+A G YP+E+V TM++IA++AE+ +N+ + +++ ++ ++ IS Sbjct: 301 IFDGTDAIMLSGESANGSYPIEAVTTMAKIAQEAENEINYDKFLAERKGNEKKNTSDVIS 360 Query: 360 QSVAISALDLNAKAIISSTVTGHTARVVSKYRPKSQIIAVTTQERTMRQLALVWGVTPVH 419 +A DL A AII++T TG TAR VSKYRPK+ +IAVT E+ R+LA+ WGV P+ Sbjct: 361 LGTCTAAADLEASAIITATQTGSTARTVSKYRPKAPVIAVTPSEKVARKLAMSWGVHPII 420 Query: 420 GLEATSTDELLETALKGGKASGLVKAGDLVVITAGIPLGRSGSTNLVKVDTI 471 + STDEL+ T++ +G V+ GDLVV+ AG+P SG+TN++KV + Sbjct: 421 SDKFGSTDELISTSVDKALEAGYVQKGDLVVVAAGVPTNVSGTTNMLKVQVV 472 >VC0485 [G] COG0469 Pyruvate kinase Length = 470 Score = 485 bits (1249), Expect = e-137, Method: Compositional matrix adjust. Identities = 254/473 (53%), Positives = 333/473 (70%), Gaps = 5/473 (1%) Query: 1 MRKSKIVCTIGPASESLENIKKLILAGMNVARLNFSHGDFEEHGNRIKTIRQASQELGKT 60 M+K+KIVCTIGP +ES+E + +L+ AGMNV RLNFSHGD+ EHG RI R+ + GK Sbjct: 1 MKKTKIVCTIGPKTESVEKLTELVNAGMNVMRLNFSHGDYVEHGTRITNFRKVMEVTGKQ 60 Query: 61 VAILLDTKGPEIRTGKLEV-EPIELVQDEYLTLTTE-EILGDQNRISVTYADLPSDVQVG 118 +AILLDTKGPEIRT KLE + ++LV + T TT+ +++G++ R++VTY+ D+ VG Sbjct: 61 LAILLDTKGPEIRTIKLENGDDVDLVAGQEFTFTTDTKVVGNKERVAVTYSGFAKDLNVG 120 Query: 119 STILIDDGLIGLTVVDIQGTEIKTRIVNGGTIKSKKGVNVPGVAISLPGITEKDTNDIIF 178 + IL+DDGLI + V+ TE+K +++N G + KGVN+PGV+++LP ++EKD ND+ F Sbjct: 121 NRILVDDGLIEMEVLATTDTEVKCKVLNNGALGENKGVNLPGVSVNLPALSEKDKNDLKF 180 Query: 179 GIEQDIDFIAASFVRKASDVQEIRALLEKHNASHIQIISKIENQQGVDNLDEILAVSDGL 238 G EQ +DF+AASF+RK SDV+EIR +L H +IQIISKIENQ+G+DN DEIL +SDG+ Sbjct: 181 GCEQGVDFVAASFIRKGSDVKEIREVLASHGGQNIQIISKIENQEGLDNFDEILELSDGI 240 Query: 239 MVARGDLGVEIPAEDVPLAQKLMIQKCNIAGKPVITATQMLDSMQRNPRPTRAEASDVAN 298 MVARGDLGVEIPAE+V AQK+MI+KCN A K VITATQMLDSM +NPRPTRAEA DVAN Sbjct: 241 MVARGDLGVEIPAEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVAN 300 Query: 299 AIFDGTDAIMLSGETAAGKYPVESVLTMSRIAEKAESALNHREIFMKQQIAQETTVTEAI 358 AI DGTDA+MLSGETA GKYPVE+V M++IAE+ + L + + +TEA+ Sbjct: 301 AIMDGTDAVMLSGETAKGKYPVEAVKIMAQIAERTDPVLKAE--LGSRLDSPRLRITEAV 358 Query: 359 SQSVAISALDLNAKAIISSTVTGHTARVVSKYRPKSQIIAVTTQERTMRQLALVWGVTPV 418 + +A L A II +T G +AR V KY P + IIAVTT ++T QL L GVTPV Sbjct: 359 CKGAVDTAEKLAAPLIIVATEAGKSARSVRKYFPTANIIAVTTNKKTAAQLVLSKGVTPV 418 Query: 419 HGLEATSTDELLETALKGGKASGLVKAGDLVVITAGIPLGRSGSTNLVKVDTI 471 +TD + SGL K GD+VV+ +G L SG+TN V + Sbjct: 419 VVDAIDNTDAFYHLGKEIALQSGLGKKGDIVVMVSG-ALVASGTTNTASVHVL 470 >ZpykF [G] COG0469 Pyruvate kinase Length = 470 Score = 476 bits (1225), Expect = e-134, Method: Compositional matrix adjust. Identities = 247/473 (52%), Positives = 326/473 (68%), Gaps = 5/473 (1%) Query: 1 MRKSKIVCTIGPASESLENIKKLILAGMNVARLNFSHGDFEEHGNRIKTIRQASQELGKT 60 M+K+KIVCTIGP +ES E + K++ AGMNV RLNFSHGD+ EHG RI+ +R + GKT Sbjct: 1 MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHGQRIQNLRNVMSKTGKT 60 Query: 61 VAILLDTKGPEIRTGKLEV-EPIELVQDEYLTLTTEE-ILGDQNRISVTYADLPSDVQVG 118 AILLDTKGPEIRT KLE + L + T TT++ ++G+ ++VTY +D+ VG Sbjct: 61 AAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGNSEMVAVTYEGFTTDLSVG 120 Query: 119 STILIDDGLIGLTVVDIQGTEIKTRIVNGGTIKSKKGVNVPGVAISLPGITEKDTNDIIF 178 +T+L+DDGLIG+ V I+G ++ +++N G + KGVN+PGV+I+LP + EKD D+IF Sbjct: 121 NTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNLPGVSIALPALAEKDKQDLIF 180 Query: 179 GIEQDIDFIAASFVRKASDVQEIRALLEKHNASHIQIISKIENQQGVDNLDEILAVSDGL 238 G EQ +DF+AASF+RK SDV EIR L+ H +I IISKIENQ+G++N DEIL SDG+ Sbjct: 181 GCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHIISKIENQEGLNNFDEILEASDGI 240 Query: 239 MVARGDLGVEIPAEDVPLAQKLMIQKCNIAGKPVITATQMLDSMQRNPRPTRAEASDVAN 298 MVARGDLGVEIP E+V AQK+MI+KC A K VITATQMLDSM +NPRPTRAEA DVAN Sbjct: 241 MVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVITATQMLDSMIKNPRPTRAEAGDVAN 300 Query: 299 AIFDGTDAIMLSGETAAGKYPVESVLTMSRIAEKAESALNHREIFMKQQIAQETTVTEAI 358 AI DGTDA+MLSGE+A GKYP+E+V M+ I E+ + +N R F ++ +TEA+ Sbjct: 301 AILDGTDAVMLSGESAKGKYPLEAVSIMATICERTDRVMNSRLEFNNDN--RKLRITEAV 358 Query: 359 SQSVAISALDLNAKAIISSTVTGHTARVVSKYRPKSQIIAVTTQERTMRQLALVWGVTPV 418 + +A L+A I+ +T G +AR V KY P + I+A+TT E+T QL L GV P Sbjct: 359 CRGAVETAEKLDAPLIVVATQGGKSARAVRKYFPDATILALTTNEKTAHQLVLSKGVVPQ 418 Query: 419 HGLEATSTDELLETALKGGKASGLVKAGDLVVITAGIPLGRSGSTNLVKVDTI 471 E TSTD+ + SGL GD+VV+ +G L SG+TN V + Sbjct: 419 LVKEITSTDDFYRLGKELALQSGLAHKGDVVVMVSG-ALVPSGTTNTASVHVL 470 >pykF [G] COG0469 Pyruvate kinase Length = 470 Score = 476 bits (1225), Expect = e-134, Method: Compositional matrix adjust. Identities = 247/473 (52%), Positives = 326/473 (68%), Gaps = 5/473 (1%) Query: 1 MRKSKIVCTIGPASESLENIKKLILAGMNVARLNFSHGDFEEHGNRIKTIRQASQELGKT 60 M+K+KIVCTIGP +ES E + K++ AGMNV RLNFSHGD+ EHG RI+ +R + GKT Sbjct: 1 MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHGQRIQNLRNVMSKTGKT 60 Query: 61 VAILLDTKGPEIRTGKLEV-EPIELVQDEYLTLTTEE-ILGDQNRISVTYADLPSDVQVG 118 AILLDTKGPEIRT KLE + L + T TT++ ++G+ ++VTY +D+ VG Sbjct: 61 AAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGNSEMVAVTYEGFTTDLSVG 120 Query: 119 STILIDDGLIGLTVVDIQGTEIKTRIVNGGTIKSKKGVNVPGVAISLPGITEKDTNDIIF 178 +T+L+DDGLIG+ V I+G ++ +++N G + KGVN+PGV+I+LP + EKD D+IF Sbjct: 121 NTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNLPGVSIALPALAEKDKQDLIF 180 Query: 179 GIEQDIDFIAASFVRKASDVQEIRALLEKHNASHIQIISKIENQQGVDNLDEILAVSDGL 238 G EQ +DF+AASF+RK SDV EIR L+ H +I IISKIENQ+G++N DEIL SDG+ Sbjct: 181 GCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHIISKIENQEGLNNFDEILEASDGI 240 Query: 239 MVARGDLGVEIPAEDVPLAQKLMIQKCNIAGKPVITATQMLDSMQRNPRPTRAEASDVAN 298 MVARGDLGVEIP E+V AQK+MI+KC A K VITATQMLDSM +NPRPTRAEA DVAN Sbjct: 241 MVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVITATQMLDSMIKNPRPTRAEAGDVAN 300 Query: 299 AIFDGTDAIMLSGETAAGKYPVESVLTMSRIAEKAESALNHREIFMKQQIAQETTVTEAI 358 AI DGTDA+MLSGE+A GKYP+E+V M+ I E+ + +N R F ++ +TEA+ Sbjct: 301 AILDGTDAVMLSGESAKGKYPLEAVSIMATICERTDRVMNSRLEFNNDN--RKLRITEAV 358 Query: 359 SQSVAISALDLNAKAIISSTVTGHTARVVSKYRPKSQIIAVTTQERTMRQLALVWGVTPV 418 + +A L+A I+ +T G +AR V KY P + I+A+TT E+T QL L GV P Sbjct: 359 CRGAVETAEKLDAPLIVVATQGGKSARAVRKYFPDATILALTTNEKTAHQLVLSKGVVPQ 418 Query: 419 HGLEATSTDELLETALKGGKASGLVKAGDLVVITAGIPLGRSGSTNLVKVDTI 471 E TSTD+ + SGL GD+VV+ +G L SG+TN V + Sbjct: 419 LVKEITSTDDFYRLGKELALQSGLAHKGDVVVMVSG-ALVPSGTTNTASVHVL 470 Score = 476 bits (1225), Expect = e-134, Method: Compositional matrix adjust. Identities = 247/473 (52%), Positives = 326/473 (68%), Gaps = 5/473 (1%) Query: 1 MRKSKIVCTIGPASESLENIKKLILAGMNVARLNFSHGDFEEHGNRIKTIRQASQELGKT 60 M+K+KIVCTIGP +ES E + K++ AGMNV RLNFSHGD+ EHG RI+ +R + GKT Sbjct: 1 MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHGQRIQNLRNVMSKTGKT 60 Query: 61 VAILLDTKGPEIRTGKLEV-EPIELVQDEYLTLTTEE-ILGDQNRISVTYADLPSDVQVG 118 AILLDTKGPEIRT KLE + L + T TT++ ++G+ ++VTY +D+ VG Sbjct: 61 AAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGNSEMVAVTYEGFTTDLSVG 120 Query: 119 STILIDDGLIGLTVVDIQGTEIKTRIVNGGTIKSKKGVNVPGVAISLPGITEKDTNDIIF 178 +T+L+DDGLIG+ V I+G ++ +++N G + KGVN+PGV+I+LP + EKD D+IF Sbjct: 121 NTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNLPGVSIALPALAEKDKQDLIF 180 Query: 179 GIEQDIDFIAASFVRKASDVQEIRALLEKHNASHIQIISKIENQQGVDNLDEILAVSDGL 238 G EQ +DF+AASF+RK SDV EIR L+ H +I IISKIENQ+G++N DEIL SDG+ Sbjct: 181 GCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHIISKIENQEGLNNFDEILEASDGI 240 Query: 239 MVARGDLGVEIPAEDVPLAQKLMIQKCNIAGKPVITATQMLDSMQRNPRPTRAEASDVAN 298 MVARGDLGVEIP E+V AQK+MI+KC A K VITATQMLDSM +NPRPTRAEA DVAN Sbjct: 241 MVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVITATQMLDSMIKNPRPTRAEAGDVAN 300 Query: 299 AIFDGTDAIMLSGETAAGKYPVESVLTMSRIAEKAESALNHREIFMKQQIAQETTVTEAI 358 AI DGTDA+MLSGE+A GKYP+E+V M+ I E+ + +N R F ++ +TEA+ Sbjct: 301 AILDGTDAVMLSGESAKGKYPLEAVSIMATICERTDRVMNSRLEFNNDN--RKLRITEAV 358 Query: 359 SQSVAISALDLNAKAIISSTVTGHTARVVSKYRPKSQIIAVTTQERTMRQLALVWGVTPV 418 + +A L+A I+ +T G +AR V KY P + I+A+TT E+T QL L GV P Sbjct: 359 CRGAVETAEKLDAPLIVVATQGGKSARAVRKYFPDATILALTTNEKTAHQLVLSKGVVPQ 418 Query: 419 HGLEATSTDELLETALKGGKASGLVKAGDLVVITAGIPLGRSGSTNLVKVDTI 471 E TSTD+ + SGL GD+VV+ +G L SG+TN V + Sbjct: 419 LVKEITSTDDFYRLGKELALQSGLAHKGDVVVMVSG-ALVPSGTTNTASVHVL 470 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.131 0.352 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,441,156 Number of extensions: 1268347 Number of successful extensions: 3565 Number of sequences better than 1.0e-05: 29 Number of HSP's gapped: 3483 Number of HSP's successfully gapped: 29 Length of query: 474 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 364 Effective length of database: 25,691,691 Effective search space: 9351775524 Effective search space used: 9351775524 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 118 (50.1 bits)