BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0333.0009.p (177 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2288 [R] COG0824 Predicted thioesterase 99 4e-21 SA1185 [R] COG0824 Predicted thioesterase 98 6e-21 BS_ynePm [R] COG0824 Predicted thioesterase 97 2e-20 DR0902 [R] COG0824 Predicted thioesterase 88 8e-18 CAC0271 [R] COG0824 Predicted thioesterase 82 3e-16 >BH2288 [R] COG0824 Predicted thioesterase Length = 143 Score = 99.0 bits (245), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 56/159 (35%), Positives = 79/159 (49%), Gaps = 29/159 (18%) Query: 15 TTFRVRYQETDQMGVVYHANYLSWFESGRTEMFRELGFAYRSLENMGVLLPVTTADLQFK 74 T VRY ETDQMGVV+H+NY WFE GR E+ +E+GF Y +E GVL PV +K Sbjct: 9 TAIDVRYAETDQMGVVHHSNYFIWFELGRGELIKEMGFHYADMEKDGVLSPVADIHASYK 68 Query: 75 SPARYDDLVAVYARLTTFSALRVVYEYEIRRLAGTETGDPEAFGLAGGSAPADVEQLPGE 134 P Y + V ++ + + LRVVY YE+ G Sbjct: 69 RPVHYGETVTIHTWVEQYDGLRVVYGYEVVNGEGV------------------------- 103 Query: 135 LLVTGSTSHVWLNTD-WKPVRLDRVLP---ELFLAITMA 169 VTGS++HV + D ++P+ + + P E +L IT + Sbjct: 104 TCVTGSSTHVCVKKDTFRPIAIRKHFPAWHEAYLRITAS 142 >SA1185 [R] COG0824 Predicted thioesterase Length = 155 Score = 98.2 bits (243), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 58/166 (34%), Positives = 80/166 (48%), Gaps = 28/166 (16%) Query: 15 TTFRVRYQETDQMGVVYHANYLSWFESGRTEMFRELGFAYRSLENMGVLLPVTTADLQFK 74 T RY ETD+MGV+YH NY +WFE R + +LGF+Y +E G++ PVT ++ +K Sbjct: 6 TEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFSYADMEKQGIISPVTDLNVNYK 65 Query: 75 SPARYDDLVAVYARLTTFSALRVVYEYEIRRLAGTETGDPEAFGLAGGSAPADVEQLPGE 134 Y + V V + +S LR VY+YEI GE Sbjct: 66 KSIFYPEKVKVKTWVEKYSRLRSVYKYEIF-------------------------NEKGE 100 Query: 135 LLVTGSTSHVWLNTD-WKPVRLDRVLPELFLAIT--MALREEGGAV 177 L TGST + + D +KP+RLDR P+ A + L EG V Sbjct: 101 LATTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTV 146 >BS_ynePm [R] COG0824 Predicted thioesterase Length = 129 Score = 96.7 bits (239), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 49/154 (31%), Positives = 77/154 (50%), Gaps = 26/154 (16%) Query: 19 VRYQETDQMGVVYHANYLSWFESGRTEMFRELGFAYRSLENMGVLLPVTTADLQFKSPAR 78 +RY ETDQMG+VYHANYL W E GRT + ++LGF Y +E GVL PV ++ +K P Sbjct: 1 MRYAETDQMGIVYHANYLVWMEVGRTALIKDLGFLYSDMEKKGVLSPVVDINISYKKPLH 60 Query: 79 YDDLVAVYARLTTFSALRVVYEYEIRRLAGTETGDPEAFGLAGGSAPADVEQLPGELLVT 138 Y + V+ + ++ + VY Y I A GEL + Sbjct: 61 YGETAVVHTWIEDYNGFKTVYGYHIYNPA-------------------------GELSIK 95 Query: 139 GSTSHVWLNTD-WKPVRLDRVLPELFLAITMALR 171 ++SH+ ++ + +KP++ + P+ A A + Sbjct: 96 ATSSHICVDKESFKPIQFRKAFPDWHTAYEKAKK 129 >DR0902 [R] COG0824 Predicted thioesterase Length = 142 Score = 87.8 bits (216), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 51/151 (33%), Positives = 72/151 (47%), Gaps = 22/151 (14%) Query: 13 HGTTFRVRYQETDQMGVVYHANYLSWFESGRTEMFRELGFAYRSLENMGVLLPVTTADLQ 72 H T RVRY ETD M V +HA Y WFE RTE+ LG Y +E G L ++ +Q Sbjct: 9 HHTELRVRYAETDAMAVAHHATYPVWFEVARTELMHALGLPYTEMETRGYYLMLSGLHVQ 68 Query: 73 FKSPARYDDLVAVYARLTTFSALRVVYEYEIRRLAGTETGDPEAFGLAGGSAPADVEQLP 132 ++ ARYDD + + R+T + + + YE+ R+ T Sbjct: 69 YRRAARYDDRLDITTRITEIRSRTLKFAYEVHRIGADGT--------------------- 107 Query: 133 GELLVTGSTSHVWLNTDWKPVRL-DRVLPEL 162 ELL TG T H+ + ++P R+ D VL L Sbjct: 108 RELLATGETHHIATDHQYRPSRMPDDVLALL 138 >CAC0271 [R] COG0824 Predicted thioesterase Length = 138 Score = 82.4 bits (202), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 42/149 (28%), Positives = 75/149 (50%), Gaps = 25/149 (16%) Query: 15 TTFRVRYQETDQMGVVYHANYLSWFESGRTEMFRELGFAYRSLENMGVLLPVTTADLQFK 74 T +VRY ETD+MG+VYHANY +FE+ R ++ G Y +E++G+++P+ ++ Sbjct: 6 TRIKVRYAETDKMGIVYHANYYVYFEAAREDLIEGAGIKYSDMEDIGIMMPLVETKCKYH 65 Query: 75 SPARYDDLVAVYARLTTFSALRVVYEYEIRRLAGTETGDPEAFGLAGGSAPADVEQLPGE 134 A+Y D + V L S ++V Y + R + G+ Sbjct: 66 EGAKYGDYILVETTLGKLSPIKVEINYRVLR------------------------ESDGK 101 Query: 135 LLVTGSTSHVWLNT-DWKPVRLDRVLPEL 162 LL G T+ V+++ ++K ++L + PE+ Sbjct: 102 LLAEGQTTQVFVDAQNFKIIKLMKSYPEV 130 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 14,028,720 Number of extensions: 596852 Number of successful extensions: 902 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 897 Number of HSP's successfully gapped: 26 Length of query: 177 Length of database: 38,378,431 Length adjustment: 99 Effective length of query: 78 Effective length of database: 26,960,365 Effective search space: 2102908470 Effective search space used: 2102908470 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 112 (47.8 bits)