BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0333.0006.p (371 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0463 [R] COG0628 Predicted permease 142 8e-34 BS_ytvI [R] COG0628 Predicted permease 132 1e-30 SMc00616 [R] COG0628 Predicted permease 60 8e-09 AGc2113 [R] COG0628 Predicted permease 57 4e-08 mlr7966 [R] COG0628 Predicted permease 52 2e-06 >BH0463 [R] COG0628 Predicted permease Length = 372 Score = 142 bits (359), Expect = 8e-34, Method: Compositional matrix adjust. Identities = 75/199 (37%), Positives = 114/199 (57%) Query: 131 NPDYHATIDSNISRTTETVGQAVTELITGFFNMVLKLISALPSMGSILIVIMLSAFFLST 190 P ++ I + + + L N + ++SALP ++++ I+L +F+S Sbjct: 132 EPSQQRALEEGIQQLGGQLASLLGNLGQAIANGLSHIVSALPLTLTVVVFIILGLYFISK 191 Query: 191 GWERHNAKLRALVPVPLRRPVSEIWHDLRNALFGYLRAQLVLISXXXXXXXXXXXXXXXN 250 W + R +P +RR ++ DL+ +FG++ AQ++LIS Sbjct: 192 DWNKFKMAARTRLPFIVRRRTLDVIKDLKTKVFGFITAQVILISLTALINFIGLLVLQVE 251 Query: 251 SAFAIGLTIGLVDLVPYLGVGIVLLPWAIYSYMTGNLALGIGLSVLYAVILITRQILEPK 310 F I L IGLVDL+PYLG GI+L+PW IYS +TGNL LG+GL LY + ++ RQ+ EPK Sbjct: 252 HPFTIALIIGLVDLLPYLGTGIILIPWGIYSIVTGNLFLGVGLLALYGLTVLIRQLAEPK 311 Query: 311 VLASSIGLDPLAMLIGVFA 329 VL+SS+GL+PLA LI +FA Sbjct: 312 VLSSSLGLNPLATLISLFA 330 >BS_ytvI [R] COG0628 Predicted permease Length = 371 Score = 132 bits (331), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 94/323 (29%), Positives = 148/323 (45%), Gaps = 5/323 (1%) Query: 46 MHPLVLIL---RGFKLPRWLAVSLSLLFYVGGTALVLTALITRLVKELIGLLQTFDLHTD 102 +HP+V L GF PR + V L F++ VLT L+ +V L +T H Sbjct: 46 IHPVVDYLDKVTGF--PRTINVLGVLAFFLLAAFGVLTILVAEIVTGTAYLAKTLPPHIS 103 Query: 103 QWRELLLSISRNASXXXXXXXXXXFYHDNPDYHATIDSNISRTTETVGQAVTELITGFFN 162 + + F + A+I ++I ++ + L++ Sbjct: 104 TFISYCEKLFTTHIQPLYNELTLLFQELETNQQASIVTHIQTLGDSAAKNAGLLLSHILE 163 Query: 163 MVLKLISALPSMGSILIVIMLSAFFLSTGWERHNAKLRALVPVPLRRPVSEIWHDLRNAL 222 M+ + + LP+ ++LI +L+ FF++ W + A L ++P + I +L+ A+ Sbjct: 164 MIPRFFALLPNTAAVLIFSLLATFFMTKDWHKLKAMLVLILPDRVTANSKAISSELKKAM 223 Query: 223 FGYLRAQLVLISXXXXXXXXXXXXXXXNSAFAIGLTIGLVDLVPYLGVGIVLLPWAIYSY 282 G+++AQ VL+ A I IGLVDL+PYLG G V +PW +Y Sbjct: 224 TGFIKAQAVLVFITMVIVFIGLSLLKVEHAATIAFLIGLVDLLPYLGAGSVFVPWILYLS 283 Query: 283 MTGNLALGIGLSVLYAVILITRQILEPKVLASSIGLDPLAMLIGVFAXXXXXXXXXXXXX 342 +TG L IG+ +LY V+LI RQ+ EPK+L+ SIG+DPLA LI +FA Sbjct: 284 ITGQLPQAIGIGILYLVVLIQRQLTEPKILSKSIGIDPLATLIALFAGFKLFGFLGLIAG 343 Query: 343 XXXXXXXDAFHRAGVFRALHSYI 365 AF G + + SYI Sbjct: 344 PAVLVIIQAFITTGALKEIWSYI 366 >SMc00616 [R] COG0628 Predicted permease Length = 371 Score = 59.7 bits (143), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 51/205 (24%), Positives = 95/205 (46%), Gaps = 11/205 (5%) Query: 132 PDYHA----TIDSNISRTTETVGQAVTELITGFFNMVLKLISALPSMGSILIVIMLSAFF 187 PD+ A TI N ++ E + L +N L L++ L S+ ++ + AF+ Sbjct: 119 PDWLAGQMTTIKQNSAKLLEQGASFLGTLFQQIWNSGLALLNIL----SLFVITPVVAFY 174 Query: 188 LSTGWERHNAKLRALVPVPLRRPVSEIWHDLRNALFGYLRAQLVLISXXXXXXXXXXXXX 247 L W+R ++ + +P V +I D+ + G++R Q L Sbjct: 175 LLLDWDRMVDRVDSWIPRDYVEVVRQIARDMNTTIAGFVRGQGSLCLILGLYYGIGLTMV 234 Query: 248 XXNSAFAIGLTIGLVDLVPYLG--VGIVL-LPWAIYSYMTGNLALGIGLSVLYAVILITR 304 N IGL G++ +PY+G VG+VL L A+ + + + + L+V ++ + Sbjct: 235 GLNFGLLIGLFAGMISFIPYVGSLVGLVLALGVALVQFWPDYVWILLVLAVFFSGQFLEG 294 Query: 305 QILEPKVLASSIGLDPLAMLIGVFA 329 IL+PK++ S+GL P+ ++ + A Sbjct: 295 NILQPKLVGKSVGLHPVWLMFALLA 319 >AGc2113 [R] COG0628 Predicted permease Length = 218 Score = 57.4 bits (137), Expect = 4e-08, Method: Compositional matrix adjust. Identities = 0/148 (0%), Positives = 0/148 (0%), Gaps = 3/148 (2%) Query: 185 AFFLSTGWERHNAKLRALVPVPLRRPVSEIWHDLRNALFGYLRAQLVLISXXXXXXXXXX 244 Sbjct: 172 231 Query: 245 XXXXXNSAFAIGLTIGLVDLVPYLG--VGIVL-LPWAIYSYMTGNLALGIGLSVLYAVIL 301 Sbjct: 232 291 Query: 302 ITRQILEPKVLASSIGLDPLAMLIGVFA 329 Sbjct: 292 319 >mlr7966 [R] COG0628 Predicted permease Length = 366 Score = 52.0 bits (123), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 39/148 (26%), Positives = 69/148 (46%), Gaps = 3/148 (2%) Query: 185 AFFLSTGWERHNAKLRALVPVPLRRPVSEIWHDLRNALFGYLRAQLVLISXXXXXXXXXX 244 AF++ W+R A + + VP V I D+ A G++R Q L Sbjct: 155 AFYMLLDWDRMVAVIDSWVPRDNVATVRAIARDINTATAGFVRGQGTLCLVLGAMYATGL 214 Query: 245 XXXXXNSAFAIGLTIGLVDLVPYLG--VGIVLLPWAIYSYMTGNLALGIGLSVLYAV-IL 301 N A IGL GL+ +PY+G G+VL + + + + ++V++ + Sbjct: 215 TLTGLNFAILIGLFAGLISFIPYVGSLTGLVLAVGVAFVQFWPDWTMIVAVAVVFFIGQF 274 Query: 302 ITRQILEPKVLASSIGLDPLAMLIGVFA 329 I IL+P+++ S+GL P+ ++ +FA Sbjct: 275 IEGNILQPRLVGKSVGLHPVWLMFALFA 302 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.143 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,881,815 Number of extensions: 699858 Number of successful extensions: 2239 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 2229 Number of HSP's successfully gapped: 26 Length of query: 371 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 263 Effective length of database: 25,922,359 Effective search space: 6817580417 Effective search space used: 6817580417 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 116 (49.3 bits)