BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0333.0003.p (431 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0972 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase 201 2e-51 XF2596 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase 154 4e-37 MTH1844 [K] COG4742 Predicted transcriptional regulator 145 1e-34 MTH1841 [G] COG0524 Sugar kinases, ribokinase family 145 1e-34 MTH184 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase 145 1e-34 >CAC0972 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Length = 334 Score = 201 bits (510), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 136/402 (33%), Positives = 198/402 (49%), Gaps = 75/402 (18%) Query: 31 IPFIEGDGTGRDIWKASKRVLDAAVAKAYGGKKQIAWYEVFAGEKAFNTYGEWLPNDTLE 90 I I GDG G ++ A+K+V++AA I W V AG K + YG LP LE Sbjct: 7 ITLIPGDGIGPEVTGAAKKVIEAAGV-------SITWDIVEAGAKVMDEYGTPLPEYVLE 59 Query: 91 AIREYIVAIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPVRYFDGVPSPVKHPELVDMV 150 +I++ +A+KGP+TTP+G G RS+NVALRQ +LY +RP++ ++G+P+ K VD++ Sbjct: 60 SIKKNKIALKGPITTPVGSGFRSVNVALRQTFNLYANVRPIKTYEGIPTRYKD---VDLI 116 Query: 151 IFRENTEDIYAGIEYKEGSAEVKKVIEFLQNELGVNKIRFPETSGIGIKPVSSEGSKRLV 210 I RENTED+YAGIE+K G + + IK ++ E S+R+ Sbjct: 117 IVRENTEDLYAGIEHKIG-----------------------DYAAESIKIITREASERIA 153 Query: 211 RSAVEYAIKHGRKSVTLVHKGNIMKFTEGAFKNWGYEVAEQEFGDKVFTWNQYDAIXXXX 270 A + A+K RK VT VHK NIMKF++G F + +VAE + +K F DA+ Sbjct: 154 DFAFDMAVKQNRKKVTAVHKANIMKFSDGLFLDCARKVAEG-YKEKEFEDMIVDAMS--- 209 Query: 271 XXXXXXXXXXXXXXSGXXXXXXXXXXXXLQQVLTRPTDFDVIATLNLNGDYLSDALXXXX 330 +++ P +DV+ NL GD LSD Sbjct: 210 -----------------------------MKLVQNPEKYDVLVMPNLYGDILSD--MAAG 238 Query: 331 XXXXXXXXXXXNYVTGHAIFEATHGTAPKYADKDVVNPGSVILSGVMLLEHLGWQEAADL 390 N +IFE+ HG+AP A K++ NP + ILSGVM+L ++G EAAD Sbjct: 239 LVGGLGVAPGANIGKEISIFESIHGSAPDIAGKNIANPTAAILSGVMMLRYIGELEAADK 298 Query: 391 IYKGMSTAINNKT-VTYDFARQMEGATELKCSAFADEIISHL 431 I + + T VT D FA+E+I ++ Sbjct: 299 IDNAIKVVLKEGTKVTSDLGGNA------TTDEFANEVIKNI 334 >XF2596 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Length = 335 Score = 154 bits (388), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 116/372 (31%), Positives = 163/372 (43%), Gaps = 65/372 (17%) Query: 31 IPFIEGDGTGRDIWKASKRVLDAAVAKAYGGKKQIAWYEVFAGEKAFNTYGEWLPNDTLE 90 I I GDG G +I A+ VL+ A + + AG A +G+ LP TL Sbjct: 5 ITVIRGDGIGPEIMDATLLVLNTLQA-------NLNYEFADAGLNALEKHGDLLPEATLN 57 Query: 91 AIREYIVAIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPVRYFDGVPSPVKHPELVDMV 150 +I + VA+K PLTTP+G G S+NVALR++ DLY +RP + F S + + VD++ Sbjct: 58 SISKNKVALKSPLTTPVGEGFSSINVALRRKFDLYANVRPAKSFPNTKS--RFSDNVDLI 115 Query: 151 IFRENTEDIYAGIEYKEGSAEVKKVIEFLQNELGVNKIRFPETSGIGIKPVSSEGSKRLV 210 RENTE Y EG E+ + R V+ +GS+R+V Sbjct: 116 TVRENTEGAY----LSEGQTISP------DGEVATSSAR-----------VTRKGSERIV 154 Query: 211 RSAVEYAIKHGRKSVTLVHKGNIMKFTEGAFKNWGYEVAEQEFGDKVFTWNQYDAIXXXX 270 R A + A GRK VT VHK NI+K T G F +VA Q + + D Sbjct: 155 RYAFDLARTTGRKKVTAVHKANIIKSTSGLFLKTARDVASQ--------YPEID------ 200 Query: 271 XXXXXXXXXXXXXXSGXXXXXXXXXXXXLQQVLTRPTDFDVIATLNLNGDYLSDALXXXX 330 Q++ RP FD+I T NL GD LSD Sbjct: 201 -------------------FQEMIVDNACMQLVMRPEQFDIIVTTNLFGDILSDLC--AG 239 Query: 331 XXXXXXXXXXXNYVTGHAIFEATHGTAPKYADKDVVNPGSVILSGVMLLEHLGWQEAADL 390 N AIFEA HG+AP A + NP +++L V +L+H+G + A+ Sbjct: 240 LVGGLGLAPGANIGNEQAIFEAVHGSAPDIAGQGKANPCALLLGAVQMLDHIGQPQNAER 299 Query: 391 IYKGMSTAINNK 402 + + +N K Sbjct: 300 LRNAIVATLNAK 311 >MTH1844 [K] COG4742 Predicted transcriptional regulator Length = 268 Score = 145 bits (367), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 0/393 (0%), Positives = 0/393 (0%), Gaps = 76/393 (19%) Query: 31 IPFIEGDGTGRDIWKASKRVLDAAVAKAYGGKKQIAWYEVFAGEKAFNTYGEWLPNDTLE 90 + ++ IA G K Y + + Sbjct: 4 VDLSIFIPDSLTAETGDLKIKTYK-------VVLIARAASIFGVKRIVIYHDDADGEARF 56 Query: 91 AIREYIVAIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPVRYFDGVPSPVKHPELVDMV 150 + + + V ++ + V Sbjct: 57 IRDILTYMDTPQYLRRKVF--PIMRELKHVGILPPLRTPHHPTGKPVTG--EYRQG-LTV 111 Query: 151 IFRENTEDIYAGIE-YKEGSAEVKKVIEFLQNELGVNKIRFPETSGIGIKPVSSEGSKRL 209 + + G + ++ Sbjct: 112 KRVKKGTLVDIGADKLALCREKLTVNR-------------------------IMSFRVVR 146 Query: 210 VRSAVEYAIKHGRKSVTLVHKGNIMKFTEGAFKNWGYEVAEQEFGDKVFTWNQYDAIXXX 269 + + + + + K G +V ++ + Sbjct: 147 LGKEILIEPDEPEDRYWGYEVLDTRRNLAESLKTVGADVVVA--------TSRNAS---- 194 Query: 270 XXXXXXXXXXXXXXXSGXXXXXXXXXXXXLQQVLTRPTDFDVIATLNLNGDYLSDALXXX 329 ++ + + ++ G D Sbjct: 195 ---------------------PITSILDEVKTRMRGAREAAILFGGPYKGLPEID--ADI 231 Query: 330 XXXXXXXXXXXXNYVTGHAIFEATHGTAPKYADKDVVNPGSVILSGVMLLEHLGWQEAAD 389 Sbjct: 232 291 Query: 390 LIYKGMSTAINNKTVTYDF---ARQMEGATELK 419 Sbjct: 292 324 >MTH1841 [G] COG0524 Sugar kinases, ribokinase family Length = 268 Score = 145 bits (367), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 0/393 (0%), Positives = 0/393 (0%), Gaps = 76/393 (19%) Query: 31 IPFIEGDGTGRDIWKASKRVLDAAVAKAYGGKKQIAWYEVFAGEKAFNTYGEWLPNDTLE 90 + ++ IA G K Y + + Sbjct: 4 VDLSIFIPDSLTAETGDLKIKTYK-------VVLIARAASIFGVKRIVIYHDDADGEARF 56 Query: 91 AIREYIVAIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPVRYFDGVPSPVKHPELVDMV 150 + + + V ++ + V Sbjct: 57 IRDILTYMDTPQYLRRKVF--PIMRELKHVGILPPLRTPHHPTGKPVTG--EYRQG-LTV 111 Query: 151 IFRENTEDIYAGIE-YKEGSAEVKKVIEFLQNELGVNKIRFPETSGIGIKPVSSEGSKRL 209 + + G + ++ Sbjct: 112 KRVKKGTLVDIGADKLALCREKLTVNR-------------------------IMSFRVVR 146 Query: 210 VRSAVEYAIKHGRKSVTLVHKGNIMKFTEGAFKNWGYEVAEQEFGDKVFTWNQYDAIXXX 269 + + + + + K G +V ++ + Sbjct: 147 LGKEILIEPDEPEDRYWGYEVLDTRRNLAESLKTVGADVVVA--------TSRNAS---- 194 Query: 270 XXXXXXXXXXXXXXXSGXXXXXXXXXXXXLQQVLTRPTDFDVIATLNLNGDYLSDALXXX 329 ++ + + ++ G D Sbjct: 195 ---------------------PITSILDEVKTRMRGAREAAILFGGPYKGLPEID--ADI 231 Query: 330 XXXXXXXXXXXXNYVTGHAIFEATHGTAPKYADKDVVNPGSVILSGVMLLEHLGWQEAAD 389 Sbjct: 232 291 Query: 390 LIYKGMSTAINNKTVTYDF---ARQMEGATELK 419 Sbjct: 292 324 >MTH184 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Length = 268 Score = 145 bits (367), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 0/393 (0%), Positives = 0/393 (0%), Gaps = 76/393 (19%) Query: 31 IPFIEGDGTGRDIWKASKRVLDAAVAKAYGGKKQIAWYEVFAGEKAFNTYGEWLPNDTLE 90 + ++ IA G K Y + + Sbjct: 4 VDLSIFIPDSLTAETGDLKIKTYK-------VVLIARAASIFGVKRIVIYHDDADGEARF 56 Query: 91 AIREYIVAIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPVRYFDGVPSPVKHPELVDMV 150 + + + V ++ + V Sbjct: 57 IRDILTYMDTPQYLRRKVF--PIMRELKHVGILPPLRTPHHPTGKPVTG--EYRQG-LTV 111 Query: 151 IFRENTEDIYAGIE-YKEGSAEVKKVIEFLQNELGVNKIRFPETSGIGIKPVSSEGSKRL 209 + + G + ++ Sbjct: 112 KRVKKGTLVDIGADKLALCREKLTVNR-------------------------IMSFRVVR 146 Query: 210 VRSAVEYAIKHGRKSVTLVHKGNIMKFTEGAFKNWGYEVAEQEFGDKVFTWNQYDAIXXX 269 + + + + + K G +V ++ + Sbjct: 147 LGKEILIEPDEPEDRYWGYEVLDTRRNLAESLKTVGADVVVA--------TSRNAS---- 194 Query: 270 XXXXXXXXXXXXXXXSGXXXXXXXXXXXXLQQVLTRPTDFDVIATLNLNGDYLSDALXXX 329 ++ + + ++ G D Sbjct: 195 ---------------------PITSILDEVKTRMRGAREAAILFGGPYKGLPEID--ADI 231 Query: 330 XXXXXXXXXXXXNYVTGHAIFEATHGTAPKYADKDVVNPGSVILSGVMLLEHLGWQEAAD 389 Sbjct: 232 291 Query: 390 LIYKGMSTAINNKTVTYDF---ARQMEGATELK 419 Sbjct: 292 324 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.137 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,276,993 Number of extensions: 1203801 Number of successful extensions: 2477 Number of sequences better than 1.0e-05: 50 Number of HSP's gapped: 2350 Number of HSP's successfully gapped: 75 Length of query: 431 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 322 Effective length of database: 25,807,025 Effective search space: 8309862050 Effective search space used: 8309862050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)