BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0333.0002.p (313 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_citH [C] COG0039 Malate/lactate dehydrogenases 439 e-123 BH3158 [C] COG0039 Malate/lactate dehydrogenases 436 e-122 NMA0819 [E] COG0665 Glycine/D-amino acid oxidases (deaminating) 301 1e-81 aq_1782 [C] COG0039 Malate/lactate dehydrogenases 290 3e-78 aq_1665 [C] COG0039 Malate/lactate dehydrogenases 236 2e-62 >BS_citH [C] COG0039 Malate/lactate dehydrogenases Length = 312 Score = 439 bits (1130), Expect = e-123, Method: Compositional matrix adjust. Identities = 207/308 (67%), Positives = 257/308 (83%) Query: 5 RNKITVVGAGFTGSTTALMLAQKELGDVVLLDIPQLENPTKGKVLDMLEAGPVLKFDARI 64 R K++V+GAGFTG+TTA ++AQKEL DVVL+DIPQLENPTKGK LDMLEA PV FDA+I Sbjct: 5 RKKVSVIGAGFTGATTAFLIAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDAKI 64 Query: 65 TGTSSYEDAADSDIVIITAGIARKPGMSRDDLVNTNAGIVKSVCENVKRVAPESIVIILS 124 TGTS+YED A SDIV+ITAGIARKPGMSRDDLV+TN I++SV + + + +P+SI+++L+ Sbjct: 65 TGTSNYEDTAGSDIVVITAGIARKPGMSRDDLVSTNEKIMRSVTQEIVKYSPDSIIVVLT 124 Query: 125 NPVDAMTYVAFKTLGFPKNRVIGQSGVLDTARYCTFIAEELNVSVEDVRGFVLGGHGDDM 184 NPVDAMTY +K GFPK RVIGQSGVLDTAR+ TF+AEELN+SV+DV GFVLGGHGDDM Sbjct: 125 NPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTFVAEELNLSVKDVTGFVLGGHGDDM 184 Query: 185 VPLVRYSSVGGIPIETLIPAERIAEIVQRTRVGGGEIVSLLGNGSAYYAPAASLVQMTEA 244 VPLVRYS GGIP+ETLIP ERI IV+RTR GGGEIV+LLGNGSAYYAPAASL +M EA Sbjct: 185 VPLVRYSYAGGIPLETLIPKERIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTEMVEA 244 Query: 245 VLKDKKRILPVIALLEGEYGYDGLFMGIPALLGAEGIEKIFXXXXXXXXXXXXDKSADSV 304 +LKD++R+LP IA LEGEYGY+G+++G+P ++G G+E+I +KS +SV Sbjct: 245 ILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIVGGNGLEQIIELELTDYERAQLNKSVESV 304 Query: 305 RSVTSVVT 312 ++V V++ Sbjct: 305 KNVMKVLS 312 >BH3158 [C] COG0039 Malate/lactate dehydrogenases Length = 314 Score = 436 bits (1120), Expect = e-122, Method: Compositional matrix adjust. Identities = 209/311 (67%), Positives = 254/311 (81%) Query: 1 VAIKRNKITVVGAGFTGSTTALMLAQKELGDVVLLDIPQLENPTKGKVLDMLEAGPVLKF 60 +AIKR K++V+GAGFTG+TTALM+AQKELGDVVL+DIPQ+E PTKGK LDMLE+ PV Sbjct: 1 MAIKRRKVSVIGAGFTGATTALMVAQKELGDVVLVDIPQMEGPTKGKALDMLESTPVQGV 60 Query: 61 DARITGTSSYEDAADSDIVIITAGIARKPGMSRDDLVNTNAGIVKSVCENVKRVAPESIV 120 D ITGTSSYE DSD+V+ITAGIARKPGMSRDDLV+TNAGI+K+V + V + +P + + Sbjct: 61 DVNITGTSSYEYTKDSDVVVITAGIARKPGMSRDDLVSTNAGIMKAVTKEVVKHSPNAYI 120 Query: 121 IILSNPVDAMTYVAFKTLGFPKNRVIGQSGVLDTARYCTFIAEELNVSVEDVRGFVLGGH 180 I+L+NP DAMTY +K GFPKNRVIGQSGVLDTAR+ TF+A+ELN+SVED+ GFVLGGH Sbjct: 121 IVLTNPADAMTYTVYKESGFPKNRVIGQSGVLDTARFRTFVAQELNLSVEDITGFVLGGH 180 Query: 181 GDDMVPLVRYSSVGGIPIETLIPAERIAEIVQRTRVGGGEIVSLLGNGSAYYAPAASLVQ 240 GDDMVPL+RYS GGIP+E L+P ERI IV+RTR GGGEIV LLGNGSAYYAPAASL + Sbjct: 181 GDDMVPLIRYSYAGGIPLEKLLPQERIDAIVERTRKGGGEIVGLLGNGSAYYAPAASLAE 240 Query: 241 MTEAVLKDKKRILPVIALLEGEYGYDGLFMGIPALLGAEGIEKIFXXXXXXXXXXXXDKS 300 M EA+LKDKKR+LP IA LEGEYGY+ +++G+P +LG +GIEK+ KS Sbjct: 241 MVEAILKDKKRVLPTIAYLEGEYGYEDIYVGVPTILGGDGIEKVIELDLTDEEKATFAKS 300 Query: 301 ADSVRSVTSVV 311 +SVR+V S + Sbjct: 301 IESVRNVMSAL 311 >NMA0819 [E] COG0665 Glycine/D-amino acid oxidases (deaminating) Length = 307 Score = 301 bits (770), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 149/274 (54%), Positives = 200/274 (72%), Gaps = 2/274 (0%) Query: 7 KITVVGAGFTGSTTALMLAQKELGDVVLLDIPQLENPTKGKVLDMLEAGPVLKFDARITG 66 KI+V+GAG G+TT LA+ E G++V+ DI +E +GK LD+++AG + +D ++TG Sbjct: 3 KISVIGAGNVGATTVQRLAELEPGEIVMTDI--VEGLPQGKALDLMQAGAINGYDTQVTG 60 Query: 67 TSSYEDAADSDIVIITAGIARKPGMSRDDLVNTNAGIVKSVCENVKRVAPESIVIILSNP 126 T+ Y D DSD+VIITAGIARKPGM+R+DL+ TN+ I+ V N+ AP SIVI ++NP Sbjct: 61 TNDYADITDSDLVIITAGIARKPGMTREDLMKTNSKIIGEVSRNIAEYAPNSIVINVTNP 120 Query: 127 VDAMTYVAFKTLGFPKNRVIGQSGVLDTARYCTFIAEELNVSVEDVRGFVLGGHGDDMVP 186 +D +TYVA KT GF +V G SGVLD R+ +FIAEELN S +D+ V+GGHGD MVP Sbjct: 121 LDVITYVAMKTTGFETKKVFGMSGVLDAGRFASFIAEELNCSKKDIEAMVIGGHGDLMVP 180 Query: 187 LVRYSSVGGIPIETLIPAERIAEIVQRTRVGGGEIVSLLGNGSAYYAPAASLVQMTEAVL 246 L +Y++V GIP+ L+P E IA +V+RT GG EIV LL GSA+YAP+A++V + EAVL Sbjct: 181 LPQYTTVSGIPLPELLPEETIARLVERTVNGGAEIVGLLKQGSAFYAPSAAIVSVAEAVL 240 Query: 247 KDKKRILPVIALLEGEYGYDGLFMGIPALLGAEG 280 KD KRILP A LEG+YG +G++ G+ A LGA G Sbjct: 241 KDSKRILPTSAYLEGQYGQEGIYFGVLAKLGANG 274 >aq_1782 [C] COG0039 Malate/lactate dehydrogenases Length = 135 Score = 290 bits (741), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 0/321 (0%), Positives = 0/321 (0%), Gaps = 16/321 (4%) Query: 5 RNKITVVGAGFTGSTTALMLAQKELGDVVLLDIP-----QLENPTKGKVLDMLEAGPVLK 59 +T + + T + ++LG + + LE L Sbjct: 4 LLVLTDAYSDCEKAITYAVNFSEKLGAELDILAVLEDVYNLERANVTFGLPFPPEIKEES 63 Query: 60 FDARITGTS------SYEDAADSDIVIITAGIARKPGMSRDDLVNTNAGIVKSVCENVKR 113 + I K Sbjct: 64 KKRIERRLREVWEKLTGSTEIPGVEYRIGPLSEEVKKFVEGKGYELVVWACYPSAYLCKV 123 Query: 114 VAPESIVIILSNPVDAMTYVAFKTLGFPKNRVIGQSGVLDTARYCTFIAEELNVSVEDVR 173 Sbjct: 124 183 Query: 174 GFVLGGHGDDMVPLVRYSSVGGIPIETLIPAERIAEIVQRTRVGGGEIVSLLGNGSAYYA 233 Sbjct: 184 - 242 Query: 234 PAASLVQMTEAVLKDKKRILPVIALLEGE----YGYDGLFMGIPALLGAEGIEKIFXXXX 289 Sbjct: 243 302 Query: 290 XXXXXXXXDKSADSVRSVTSV 310 Sbjct: 303 323 >aq_1665 [C] COG0039 Malate/lactate dehydrogenases Length = 265 Score = 236 bits (603), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 0/289 (0%), Positives = 0/289 (0%), Gaps = 10/289 (3%) Query: 3 IKRNKITVVGAGFTGSTTALMLAQKELGDVVLL-DIPQLENPTKGKVLDMLEAGPVLKFD 61 + A + L + D V E A Sbjct: 11 GIVGFGNMGQAFALCFSKKLGKENIIVTDKVQEKRNLATEMGIAFASDVKFLADNSDVVL 70 Query: 62 ARITGTS----SYEDAADSDIVIITAGIARKPGMSRDDLVNTNAGIVKSVCENVKRVAPE 117 + + I++ M + + V Sbjct: 71 VAVKPKDSQEVLQKLKDYKGIILSIMAGVSIEKMEKILGKDKKIVRVMPNVNVAVGSGVM 130 Query: 118 SIVIILSNPVDAMTYVAFKTLGFPKNRVIGQSGVLDTARYCTFIAEELNVSVEDVRGFVL 177 +I + + + V L I + + ++ + + Sbjct: 131 AITDNGNLSEEERSKVEELLLSCGTLYRIEERLFDAFTALAGSGPAFVFSFIDALALAGV 190 Query: 178 GGHGDDMVPLVRYSSVGGIPIETLIPAERIAEIVQRTRVGGGEIVSLLGNGSAYYAPAAS 237 L + L + + G Sbjct: 191 HQGFSYEQALRIALDTVMGSAKLLKEFQVNPNELIAKVTSPGGTTIEGIK-YLEEKGFKG 249 Query: 238 LVQMTEAVLKDKKRILPVIALLEGE----YGYDGLFMGIPALLGAEGIE 282 Sbjct: 250 298 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,866,555 Number of extensions: 891371 Number of successful extensions: 2431 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 2379 Number of HSP's successfully gapped: 32 Length of query: 313 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 207 Effective length of database: 26,153,027 Effective search space: 5413676589 Effective search space used: 5413676589 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)