BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0331.0007.p
         (218 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

mlr0926 [H] COG0262 Dihydrofolate reductase                           142   3e-34
SMc02439 [H] COG0262 Dihydrofolate reductase                           73   4e-13
BS_yyaP [H] COG0262 Dihydrofolate reductase                            70   3e-12
mlr2644 [H] COG0262 Dihydrofolate reductase                            59   6e-09
mll8258 [H] COG0262 Dihydrofolate reductase                            58   1e-08

>mlr0926 [H] COG0262 Dihydrofolate reductase
          Length = 86

 Score =  142 bits (359), Expect = 3e-34,   Method: Compositional matrix adjust.
 Identities = 0/155 (0%), Positives = 0/155 (0%), Gaps = 5/155 (3%)

Query: 1   MAKIVFALNQSLDGFV---DHDHPAFQPDPVLFRHFIDDVRGLAGSLYGRRMYEIMRYWD 57
           M+        + D +                L        +           Y ++    
Sbjct: 1   MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASDLVSRKNVEYLVVEAAG 60

Query: 58  GDHMEWDAPE--REYAAAWQSKPKWVVSRTLKSVGPNASLIEGDLAAAIRGLKAQHEGEI 115
                                                                       
Sbjct: 61  ETRELRLDKITS                                                 120

Query: 116 EVAGPELAHSLTELGLVDEYRLYLHPVVLGHGKPF 150
                                              
Sbjct: 121                                     155


>SMc02439 [H] COG0262 Dihydrofolate reductase
          Length = 86

 Score = 72.8 bits (177), Expect = 4e-13,   Method: Compositional matrix adjust.
 Identities = 0/156 (0%), Positives = 0/156 (0%), Gaps = 13/156 (8%)

Query: 1   MAKIVFALNQSLDGFVD---HDHPAFQPDPVLFRHFIDDVRGLAGSL-YGRRMYEIMRYW 56
           M+        + D + +              L +         +  +      Y ++   
Sbjct: 1   MSMNDTYQPINCDDYDNLELACQHHLMLTLEL-KDGEKLQAKASDLVSRKNVEYLVVEAA 59

Query: 57  DGDHMEWDAPEREYAAAWQSKPKWVVSRTLKSVG-PNASLIEGDLAAAIRGLKAQHEGEI 115
                                                                       
Sbjct: 60  ------                                                       113

Query: 116 EVAGP-ELAHSLTELGLVDEYRLYLHPVVLGHGKPF 150
                                               
Sbjct: 114                                      149


>BS_yyaP [H] COG0262 Dihydrofolate reductase
          Length = 86

 Score = 69.7 bits (169), Expect = 3e-12,   Method: Compositional matrix adjust.
 Identities = 0/154 (0%), Positives = 0/154 (0%), Gaps = 11/154 (7%)

Query: 3   KIVFALNQSLDGFVDH-----DHPAFQPDPVLFRHFIDDVRGLAGSLYGRRMYEIMRYWD 57
            I      +L+    H                             ++    +       +
Sbjct: 9   PINCDDYDNLELACQHHLMLTLELKDGEK----LQAKASDLVSRKNVEYLVVEAAGETRE 64

Query: 58  GDHMEWDAPEREYAAAWQSKPKWVVSRTLKSVGPNASLIEGDLAAAIRGLKAQHEGEIEV 117
               +  +                                                    
Sbjct: 65  LRLDKITS-                                                    123

Query: 118 -AGPELAHSLTELGLVDEYRLYLHPVVLGHGKPF 150
                                             
Sbjct: 124                                    157


>mlr2644 [H] COG0262 Dihydrofolate reductase
          Length = 86

 Score = 58.9 bits (141), Expect = 6e-09,   Method: Compositional matrix adjust.
 Identities = 0/155 (0%), Positives = 0/155 (0%), Gaps = 9/155 (5%)

Query: 1   MAKIVFALNQSLDGFVDHDHPAF----QPDPVLFRHFIDDVRGLAGSLYGRRMYEIM-RY 55
           M+        + D + + +            +     +        S        +    
Sbjct: 1   MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASDLVSRKNVEYLVVEAAG 60

Query: 56  WDGDHMEWDAPEREYAAAWQSKPKWVVSRTLKSVG-PNASLIEGDLAAAIRGLKAQHEGE 114
              +                                                        
Sbjct: 61  ETRELRLDKITS--                                               118

Query: 115 IEVAGP-ELAHSLTELGLVDEYRLYLHPVVLGHGK 148
                                              
Sbjct: 119                                     153


>mll8258 [H] COG0262 Dihydrofolate reductase
          Length = 86

 Score = 57.8 bits (138), Expect = 1e-08,   Method: Compositional matrix adjust.
 Identities = 0/153 (0%), Positives = 0/153 (0%), Gaps = 9/153 (5%)

Query: 1   MAKIVFALNQSLDGFVDHDH-PAFQPDPVLFRHFIDDVRGLAGSLYGRRMYEIMRYWDGD 59
           M+        + D + + +          L     + ++  A  L  R+  E +      
Sbjct: 1   MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASDLVSRKNVEYL------ 54

Query: 60  HMEWDAPEREYAAAWQSKPKWVVSRTLKSVG-PNASLIEGDLAAAIRGLKAQHEGEIEVA 118
            +E     RE      +        T+                                 
Sbjct: 55  VVEAAGETRELRLDKITSFSHPEIGTVVVSES                             114

Query: 119 GP-ELAHSLTELGLVDEYRLYLHPVVLGHGKPF 150
                                            
Sbjct: 115                                   147


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.321    0.137    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 17,088,490
Number of extensions: 736458
Number of successful extensions: 1000
Number of sequences better than 1.0e-05: 6
Number of HSP's gapped: 997
Number of HSP's successfully gapped: 6
Length of query: 218
Length of database: 38,378,431
Length adjustment: 102
Effective length of query: 116
Effective length of database: 26,614,363
Effective search space: 3087266108
Effective search space used: 3087266108
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 113 (48.1 bits)