BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0331.0003.p (246 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc1567 [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 236 2e-62 L179660 [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 234 7e-62 alr2142 [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 231 1e-60 XF1744 [R] COG4221 Short-chain alcohol dehydrogenase of unknown ... 225 4e-59 SMa2343 [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 223 2e-58 >AGc1567 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Length = 370 Score = 236 bits (603), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 14/239 (5%), Positives = 34/239 (14%), Gaps = 3/239 (1%) Query: 6 NKVVIITGASSGIGEATAKLLAQNGAKVVLAARREERLNAIVNEIKQGGGEAVSVKADVI 65 + + +G TA L E + Sbjct: 35 SHLRNAKPYRTGALGITAAELVSLLELAERGQVNSPEQARQFFETNSVPFRISPAQGKSG 94 Query: 66 SAEDMQKLAKFALNTYGRIDVWVNNAGVMPSSRLHELRVGEWDQMIDVNIKGVLYGIAAV 125 + T + + + + K GI+ Sbjct: 95 FVTAFYEPELEVSATPDDVWRYPIYRRPPELVDIDNDNRPDGFDPSYAFGKADEEGISYF 154 Query: 126 LPAMREQQSGHIINLSSTAGYQVSPTNAVYAATKFAVRAISEGLRLEESSASRIRSTLIA 185 + A + A +G + Sbjct: 155 PDRRAIDEGCLRGRGLEIAWARSKVDLFFVHVQGAARLVFPDGAIKRITY---AAKAGHV 211 Query: 186 PGLTKTELFDSITSPEVQTIANQLSGVGISPYSIARAIAFAINEPDDTLISEIMVRPTA 244 L D + + + +++ Sbjct: 212 FSPIGRLLLDRGELDPKTISMQTIRQWLADHPDEVDGVLWHNRSYIFFREADVAGLDMG 270 >L179660 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Length = 190 Score = 234 bits (598), Expect = 7e-62, Method: Compositional matrix adjust. Identities = 0/246 (0%), Positives = 0/246 (0%), Gaps = 2/246 (0%) Query: 3 NIQNKVVIITGASSGIGEATAKLLAQNGAKVVLAARREERLNAIVNEIKQGGGEAVSVKA 62 + + +I + E + A+ L EE + A +A + Sbjct: 4 FEEIEAELINQKDGNLLEIEKFKNNKEKAERTLKIANEELIKAEETADLVAYDKAKGNIS 63 Query: 63 DVISAEDMQKLAKFALNTYGRIDVWVNNAGVMPSSRLHELRVGEWDQMIDVNIKGVLYGI 122 A ++ + L + + N V + + E + + + Sbjct: 64 TSQHAIELYQKQLDKLESTPLVTKDDYNQLVSDIEKAADKLQDELNIKGAKLMAELKSLA 123 Query: 123 AAVLPAMREQQSGHIINLSSTAGYQVSPTNAVYAATKFAVRAISEGLRLEESS-ASRIRS 181 + I + AV + + Sbjct: 124 DESNAVYHKADELLRIAQYDVYKDADCLVASNGTRITRAVEYKRNNSVRNVYNFKYLGNT 183 Query: 182 TLIAPGLTKTELFDSITSPE-VQTIANQLSGVGISPYSIARAIAFAINEPDDTLISEIMV 240 Sbjct: 184 243 Query: 241 RPTALP 246 Sbjct: 244 249 >alr2142 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Length = 359 Score = 231 bits (588), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 13/242 (5%), Positives = 42/242 (17%), Gaps = 3/242 (1%) Query: 1 MENIQNKVVIITGASSGIGEATAKLLAQNGAKVVLAARREERLNAIVNEIKQGGGEAVSV 60 M+ + + ++ A V A L + Sbjct: 1 MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARL 60 Query: 61 KADVISAEDMQKLAKFALNTYGRIDVWVNNAGVMPSSRLHELRVGEWDQMIDVNIKGVLY 120 + + L + + + +H + ++ ++ Sbjct: 61 EEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVW 120 Query: 121 GIAAVLPAMREQQSGHIINLSSTAGYQVSPTNAVYAATKFAVRAISEGLRLEESSASRIR 180 + + V + FA + E+ Sbjct: 121 MKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQL---AI 177 Query: 181 STLIAPGLTKTELFDSITSPEVQTIANQLSGVGISPYSIARAIAFAINEPDDTLISEIMV 240 G + + ++ + D + + Sbjct: 178 FNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSL 237 Query: 241 RP 242 Sbjct: 238 TS 239 >XF1744 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Length = 247 Score = 225 bits (574), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 121/245 (49%), Positives = 157/245 (64%), Gaps = 2/245 (0%) Query: 4 IQNKVVIITGASSGIGEATAKLLAQNGAKVVLAARREERLNAIVNEIKQGGGEAVSVKAD 63 I++KVVIITGASSGIGEATAKLLA GAKVVL ARR + L I +EIKQ GG+ D Sbjct: 2 IKDKVVIITGASSGIGEATAKLLASQGAKVVLGARRTDNLKRIADEIKQSGGQVAYQALD 61 Query: 64 VISAEDMQKLAKFALNTYGRIDVWVNNAGVMPSSRLHELRVGEWDQMIDVNIKGVLYGIA 123 V ED ++ K A +T+GR+DV NAG+MP+S L L+ +W QM+DVN+KGVL G+A Sbjct: 62 VTRQEDNDRIVKLAQDTFGRVDVIFLNAGLMPNSPLSALKTDDWHQMVDVNVKGVLNGVA 121 Query: 124 AVLPAMREQQSGHIINLSSTAGYQVSPTNAVYAATKFAVRAISEGLRLEES-SASRIRST 182 AVLPA Q+SGH+I SS AG + P AVY TK+ VR E LR+E + + IR+ Sbjct: 122 AVLPAFTAQKSGHVITTSSVAGLKAYPGGAVYGGTKWFVRDFMEVLRMESAMEGTNIRTA 181 Query: 183 LIAPGLTKTELFDSITSPEV-QTIANQLSGVGISPYSIARAIAFAINEPDDTLISEIMVR 241 I P TEL +I+ + + + GISP IA +AFAI++P+DT ++E V Sbjct: 182 TIYPAAINTELLTTISDKTSREQMESTYDKYGISPDRIASIVAFAIDQPEDTTVNEFTVG 241 Query: 242 PTALP 246 P P Sbjct: 242 PANQP 246 >SMa2343 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Length = 240 Score = 223 bits (568), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 118/239 (49%), Positives = 155/239 (64%), Gaps = 3/239 (1%) Query: 6 NKVVIITGASSGIGEATAKLLAQNGAKVVLAARREERLNAIVNEIKQGGGEAVSVKADVI 65 +KV++ITGAS GIGE A+ L GAK++L ARR+ R+ AI EI+ GG A++ DV Sbjct: 2 DKVILITGASGGIGEGIARELGVAGAKILLGARRQARIEAIATEIRDAGGTALAQVLDVT 61 Query: 66 SAEDMQKLAKFALNTYGRIDVWVNNAGVMPSSRLHELRVGEWDQMIDVNIKGVLYGIAAV 125 + A+ A++T+GRIDV VNNAGVMP S L ++V EW++MIDVNIKGVL+GI AV Sbjct: 62 DRHSVAAFAQAAVDTWGRIDVLVNNAGVMPLSPLAAVKVDEWERMIDVNIKGVLWGIGAV 121 Query: 126 LPAMREQQSGHIINLSSTAGYQVSPTNAVYAATKFAVRAISEGLRLEESSASRIRSTLIA 185 LP M Q+SG IIN+ S V PT AVY ATKFAVRAIS+GLR E ++ IR T + Sbjct: 122 LPIMEAQRSGQIINIGSIGALSVVPTAAVYCATKFAVRAISDGLRQESTN---IRVTCVN 178 Query: 186 PGLTKTELFDSITSPEVQTIANQLSGVGISPYSIARAIAFAINEPDDTLISEIMVRPTA 244 PG+ ++EL +IT E + + + P IARA+ I P +EI +RPTA Sbjct: 179 PGVVESELAGTITHEETMAAMDTYRAIALQPADIARAVRQVIEAPQSVDTTEITIRPTA 237 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.130 0.350 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,011,081 Number of extensions: 589207 Number of successful extensions: 2118 Number of sequences better than 1.0e-05: 353 Number of HSP's gapped: 1733 Number of HSP's successfully gapped: 353 Length of query: 246 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 142 Effective length of database: 26,383,695 Effective search space: 3746484690 Effective search space used: 3746484690 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 114 (48.5 bits)