BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0327.0022.p (390 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3629 [T] COG4585 Signal transduction histidine kinase 315 9e-86 BS_degS [T] COG4585 Signal transduction histidine kinase 294 1e-79 DR0577 [T] COG4585 Signal transduction histidine kinase 128 1e-29 DR1227_2 [T] COG4585 Signal transduction histidine kinase 119 1e-26 SA2180 [T] COG4585 Signal transduction histidine kinase 108 2e-23 >BH3629 [T] COG4585 Signal transduction histidine kinase Length = 377 Score = 315 bits (807), Expect = 9e-86, Method: Compositional matrix adjust. Identities = 167/363 (46%), Positives = 248/363 (68%), Gaps = 4/363 (1%) Query: 12 AIDRVIKNTIDVMESSKYQIFEILQVARDELVALTKELQRVMEETDETLQKVDKLELQYH 71 +D++I T+D + S+ QIFEI + +R+E V+L +ELQ V + E + + D+ E+ Sbjct: 6 TLDKIIDKTLDTVGKSREQIFEISEQSRNEYVSLEQELQEVRMKVAEIIDQSDRAEVHAR 65 Query: 72 RSRIRLTEVSRDFVRYSEKDIRVAYEKATELQLELMMTREREVYLRSRRDELQMRVRSVE 131 +R RL EVS+ F RYS ++IR YE+A ELQ++L + ++ E LR RRD ++ R+ +++ Sbjct: 66 FARNRLAEVSKQFHRYSNEEIRKVYEQANELQVKLALLQQEEQQLRDRRDAIERRLLNLK 125 Query: 132 NSVERAESIGSQMSVVLEYLSGELGQVTRIVESAKNRQMIGLKIILAQEEERKRIAREIH 191 +++ERAE + QM+VV +L+G+L QV +E A+ +Q GL+II AQEEER++++REIH Sbjct: 126 DTIERAEELVGQMTVVYNFLTGDLRQVGEALEDAREKQAFGLQIIQAQEEERRKLSREIH 185 Query: 192 DGPAQMLANLVLRTEIVERMLVKQEFGLVQDEVLDLKGQVRYSLEEMRKVIFNLRPMALD 251 DGPAQM+AN++LR+E+VER+ + E+ DL+ V+ SL E+R++I++LRPMALD Sbjct: 186 DGPAQMMANVLLRSELVERIYHDKGIDEALKEIRDLRKMVKSSLAEVRRIIYDLRPMALD 245 Query: 252 DLGLIPTLRKYVHDYEEKTKIRTSFETRGKEHRLSSAMEAAVYRLVQEALSNAAKHAFPS 311 DLGLIPTL+KYV +EE T I F+ GK R +E A++RLVQEAL N KHA S Sbjct: 246 DLGLIPTLKKYVKTFEEHTGIFVDFKHIGKGERFPEHVEIALFRLVQEALQNTRKHAKAS 305 Query: 312 YVLVEITYQAQLIKIVVKDNGLGFNVKKTSEQANRESFGLVGMRERVELLEGRMEILSAE 371 +V V+I Q +V+KDNG GF+ + E SFGLVGM+ERV +L+G++ I + Sbjct: 306 HVHVKIEEQKTKFTVVIKDNGKGFDQTEKKEG----SFGLVGMKERVNMLKGQLVIRTKP 361 Query: 372 NQG 374 N G Sbjct: 362 NDG 364 >BS_degS [T] COG4585 Signal transduction histidine kinase Length = 385 Score = 294 bits (753), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 169/381 (44%), Positives = 269/381 (70%), Gaps = 8/381 (2%) Query: 10 ADAIDRVIKNTIDVMESSKYQIFEILQVARDELVALTKELQRVMEETDETLQKVDKLELQ 69 + +D ++ + ++ SK ++F+I + +R + L +EL+++ ++ E ++ DKLE+Q Sbjct: 8 SKVLDSILMKMLKTVDGSKDEVFQIGEQSRQQYEQLVEELKQIKQQVYEVIELGDKLEVQ 67 Query: 70 YHRSRIRLTEVSRDFVRYSEKDIRVAYEKATELQLELMMTREREVYLRSRRDELQMRVRS 129 +R RL+EVSR+F R+SE++IR AYEKA +LQ+EL M ++RE LR RRD+L+ R+ Sbjct: 68 TRHARNRLSEVSRNFHRFSEEEIRNAYEKAHKLQVELTMIQQREKQLRERRDDLERRLLG 127 Query: 130 VENSVERAESIGSQMSVVLEYLSGELGQVTRIVESAKNRQMIGLKIILAQEEERKRIARE 189 ++ +ER+ES+ SQ++VVL YL+ +L +V ++ A+ +Q GL+II AQEEERKR++RE Sbjct: 128 LQEIIERSESLVSQITVVLNYLNQDLREVGLLLADAQAKQDFGLRIIEAQEEERKRVSRE 187 Query: 190 IHDGPAQMLANLVLRTEIVERMLVKQ--EFGLVQDEVLDLKGQVRYSLEEMRKVIFNLRP 247 IHDGPAQMLAN+++R+E++ER+ + E G E+ +L+ VR +L E+R++I++LRP Sbjct: 188 IHDGPAQMLANVMMRSELIERIFRDRGAEDGF--QEIKNLRQNVRNALYEVRRIIYDLRP 245 Query: 248 MALDDLGLIPTLRKYVHDYEE-KTKIRTSFETRG--KEHRLSSAMEAAVYRLVQEALSNA 304 MALDDLGLIPTLRKY++ EE K++ F+ G ++ RL+ E A++RL QEA+SNA Sbjct: 246 MALDDLGLIPTLRKYLYTTEEYNGKVKIHFQCIGETEDQRLAPQFEVALFRLAQEAVSNA 305 Query: 305 AKHAFPSYVLVEITYQAQLIKIVVKDNGLGFNVKKTSEQANRESFGLVGMRERVELLEGR 364 KH+ + V++ + +++KDNG GF++K+ E+ N+ SFGL+GM+ERV+LLEG Sbjct: 306 LKHSESEEITVKVEITKDFVILMIKDNGKGFDLKEAKEKKNK-SFGLLGMKERVDLLEGT 364 Query: 365 MEILSAENQGTTIVIHIPTNV 385 M I S GT I+I +P ++ Sbjct: 365 MTIDSKIGLGTFIMIKVPLSL 385 >DR0577 [T] COG4585 Signal transduction histidine kinase Length = 590 Score = 128 bits (322), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 79/207 (38%), Positives = 122/207 (58%), Gaps = 3/207 (1%) Query: 177 LAQEEERKRIAREIHDGPAQMLANLVLRTEIVERMLVKQEFGLVQDEVLDLKGQVRYSLE 236 LA +ER RIAREIHDG AQ LA ++ ++V R L + + + +V + +R + Sbjct: 384 LAISDERARIAREIHDGVAQSLAFAAIKLDLVGRKL-RSDPDKSEADVKEASALLREQIR 442 Query: 237 EMRKVIFNLRPMALDDLGLIPTLRKYVHDYEEKTKIRTSFETRGKEHRLSSAMEAAVYRL 296 E+R+ IF LRP+ L+ GL+ T+R+YV D+ E+ +RT+ + G H LS EA V+R+ Sbjct: 443 EVRRSIFALRPVDLERYGLLETVRRYVADFGEQNGVRTNLDLTGDIH-LSPGDEAVVFRI 501 Query: 297 VQEALSNAAKHAFPSYVLVEITYQAQLIKIVVKDNGLGFNVKKTSEQ-ANRESFGLVGMR 355 +QE+L+N AKHA V V + A + + V+D+G GF+ S + ++ GL+ M+ Sbjct: 502 LQESLNNVAKHARAREVTVSLHGGANRVTLRVQDDGQGFDPDAVSGRVSSAGGLGLMQMQ 561 Query: 356 ERVELLEGRMEILSAENQGTTIVIHIP 382 ER+E G ILSA GT + +P Sbjct: 562 ERLEARGGSYRILSAPGHGTVVEAQLP 588 >DR1227_2 [T] COG4585 Signal transduction histidine kinase Length = 240 Score = 119 bits (297), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 86/240 (35%), Positives = 131/240 (54%), Gaps = 14/240 (5%) Query: 148 LEYLSGELGQVTRIVESAKNRQ-----MIGLKIILAQEEERKRIAREIHDGPAQMLANLV 202 L+ L+ E+ + + +E+A +RQ + L AQEEER+RI+RE+HD AQ+L Sbjct: 9 LQTLADEIARRGQALEAATSRQSELRSYLAL-FTQAQEEERRRISRELHDDTAQVLIATA 67 Query: 203 LRTEIVERMLVKQEFGLVQDEVLDLKGQVRYSLEEMRKVIFNLRPMALDDLGLIPTLRKY 262 R + R L G ++ D++ + ++E +R+ NLRP LDDLGL+P L Sbjct: 68 RRVARLARDLD----GPQRERADDIRADLDTAIESVRRFARNLRPSVLDDLGLLPALEWL 123 Query: 263 VHDYEEKTKIRTSFETRGKEHRLSSAMEAAVYRLVQEALSNAAKHAFPSYVLVEITYQAQ 322 + ++ E G E RLS A E V+RL QEALSN KHA + + IT+ Sbjct: 124 ASQAQTAARL----EVSGPERRLSPAAELTVFRLAQEALSNVDKHAHAASAALRITFGES 179 Query: 323 LIKIVVKDNGLGFNVKKTSEQANRESFGLVGMRERVELLEGRMEILSAENQGTTIVIHIP 382 + + V D+G GF ++ +A GL+G+RERV L G++ + SA +GTT+V +P Sbjct: 180 GVSLRVSDDGQGFTPEQAEARAQAGHLGLLGLRERVALAGGQLTVDSAPGRGTTLVFELP 239 >SA2180 [T] COG4585 Signal transduction histidine kinase Length = 344 Score = 108 bits (269), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 74/211 (35%), Positives = 114/211 (54%), Gaps = 10/211 (4%) Query: 174 KIILAQEEERKRIAREIHDGPAQMLANLVLRTEIVERMLVKQE--FGLVQDEVLDLKGQV 231 K I AQE ERKRI+RE+HD Q + N+ ++ R+L QE L++D +++ V Sbjct: 141 KTISAQENERKRISRELHDSVIQEMLNV----DVQLRLLKYQEDTTKLLED-AENIEYIV 195 Query: 232 RYSLEEMRKVIFNLRPMALDDLGLIPTLRKYVHDYEEKTKIRTSFETRGKEHRLSSAMEA 291 ++++R + LRP +LDDLGL + Y +EE I+ + + K R S +E Sbjct: 196 AKLIDDIRNMSVELRPASLDDLGLEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIET 255 Query: 292 AVYRLVQEALSNAAKHAFPSYVLVEITYQAQLIKIVVKDNGLGFNVKKTSEQANRESFGL 351 VYR+VQEA+ NA K+A + + V I + + V D G GF+ S + GL Sbjct: 256 VVYRVVQEAILNALKYADVNEINVGIRQTGRHLVAEVIDAGNGFD---PSSKPKGSGLGL 312 Query: 352 VGMRERVELLEGRMEILSAENQGTTIVIHIP 382 GM ER EL+ G + I + +GT + ++IP Sbjct: 313 YGMNERAELVSGSVNIETKIGEGTNVTLNIP 343 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.133 0.344 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,859,242 Number of extensions: 927793 Number of successful extensions: 2750 Number of sequences better than 1.0e-05: 62 Number of HSP's gapped: 2641 Number of HSP's successfully gapped: 62 Length of query: 390 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 282 Effective length of database: 25,922,359 Effective search space: 7310105238 Effective search space used: 7310105238 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)