BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0327.0012.p (363 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0958 [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotran... 235 9e-62 BS_tagO [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotran... 224 1e-58 lin2663 [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotran... 207 2e-53 L35751 [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotrans... 182 5e-46 SA0702 [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotrans... 181 1e-45 >lin0958 [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase Length = 533 Score = 235 bits (599), Expect = 9e-62, Method: Compositional matrix adjust. Identities = 11/345 (3%), Positives = 43/345 (12%), Gaps = 15/345 (4%) Query: 2 GLALLLTPLVKKFAVKIGATDVPNARKVHTKIMPRXXXXXXXXXXXXXXXXXXPIIPYDF 61 +L+ + + + + + Sbjct: 11 LFLILILCGILQIGSNGMSFWAFRDDLQRLNQVEQSNQQRAALAQT---------RAVML 61 Query: 62 TPREANFIKXXXXXXXXXXXXXXXDDRFELSAKVKLLGQIAAACIVVFGFGITVDFVNIP 121 A ++ + + + G Sbjct: 62 QASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFKSFMAMTAGNEHVRGLQKET 121 Query: 122 FNNSYSSLESWIAIPLTIFWIVGVTNAVNLIDGLDGLAAGVSGIAIATIAVMAFLMGNTM 181 + + + A + + L + Sbjct: 122 EKSFARW--HNDLEHQATWLESNQLSDFLTAPVQGSQNAFDVNFEAWQLEINHVLEAASA 179 Query: 182 VAXXXXXXXXXXXXXXXXXXHPAKIFMGDTGSXXXXXXXXXXXXXXXKQIAVVSFITPLL 241 + A + Sbjct: 180 QSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPLAIIGSHFDSIAAGNLARPIAV 239 Query: 242 IIGVPLSDTFFAIVRRKLQKKPIFAPDKGHLHHCLRELGFSHRQTVLIIYGIAGFFGILA 301 ++ F ++ + + + + + + LA Sbjct: 240 YGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIGIAEIVAGNNDLSSRTEQQAASLA 299 Query: 302 VIQTSASLYEANWVTFVVICVMLFFLQIGAEITGVISKTKRPLID 346 S V + + Sbjct: 300 QTAASMEQ----LTATVGQNADNARQASELAKNAATTAQAGGVQV 340 >BS_tagO [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase Length = 187 Score = 224 bits (572), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 0/353 (0%), Positives = 0/353 (0%), Gaps = 16/353 (4%) Query: 5 LLLTPLVKKFAVKIGATDVPNARKVHTKIMPRXXXXXXXXXXXXXXXXXXPIIPYDFTPR 64 + K ++ + A ++ + + Sbjct: 21 WGVPETDSKKLFEMICLEGQQAGLSWITVLKKRENYRACFHQFDPVKVAAM---QEEDVE 77 Query: 65 EANFIKXXXXXXXXXXXXXXXDDRFELSAKVKLLGQIAAACIVVFGFGITVDFVNIPFNN 124 A +++ V + Sbjct: 78 RL-----VQDAGIIRHRGKIQAIIGNARAYLQMEQNGEPFVDFV-WSFVNHQPQVTQATT 131 Query: 125 SYSSLESWIAIPLTIFWIVGVTNAVNLIDGLDGLAAGVSGIAIATIAVMAFLMGNTMVAX 184 Sbjct: 132 LSEIP- 190 Query: 185 XXXXXXXXXXXXXXXXXHPAKIFMGDTGSXXXXXXXXXXXXX-XXKQIAVVSFITPLLII 243 Sbjct: 191 250 Query: 244 GVPLSDTFFAIVRRKLQKKPIFAPDKGHLHHCLRELGFSHRQTVLIIYGIAGFFGILAVI 303 Sbjct: 251 310 Query: 304 QTSASLYEANWVTFVVICVMLFFLQIGAEITGVISKTKRPLIDFLLRMRVKAN 356 Sbjct: 311 ----- 358 >lin2663 [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase Length = 533 Score = 207 bits (527), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 9/355 (2%), Positives = 42/355 (11%), Gaps = 19/355 (5%) Query: 2 GLALLLTPLVKKFAVKIGATDVPNARKVHTKIMPRXXXXXXXXXXXXXXXXXXPIIPYDF 61 +L+ + + + + Sbjct: 11 LFLILILCGILQIGSNGMSFWAFRDDLQRLN-QVEQSNQQRAALAQTRAVMLQASTALNK 69 Query: 62 TPREANFIKXXXXXXXXXXXXXXXDDRFELSAKVKLLGQIAAA-CIVVFGFGITVDFVNI 120 ++ + + + Sbjct: 70 AG--------TLTALSYPADDIKTLMTTARASLTQSTTLFKSFMAMTAGN-EHVRGLQKE 120 Query: 121 PFNNSYSSLE-SWIAIPLTIFWIVGVTNAVNLIDGLDGLAAGVSGIAIATIAVMAFLMGN 179 S + + A + + L Sbjct: 121 TE---KSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFDVNFEAWQLEINHVLEAA 177 Query: 180 TMVAXXXXXXXXXXXXXXXXXXHPAKIFMGDTGSXXXXXXXXXXXXXXXKQIAVVSFITP 239 + + A Sbjct: 178 SAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPLAIIGSHFDSIAAGNLARPI 237 Query: 240 LLIIGVPLSDTFFAIVRRKLQKKPIFAPDKGHLHHCLRELGFSHRQTVLIIYGIAGFFGI 299 + ++ F ++ + + + + + + Sbjct: 238 AVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIGIAEIVAGNNDLSSRTEQQAAS 297 Query: 300 LAVIQTSASLYEANWVTFVVICVMLFFLQIGAEITGVISKTKRPLIDFLLRMRVK 354 LA V + + + ++ Sbjct: 298 LAQTAA----SMEQLTATVGQNADNARQASELAKNAATTAQAGGVQVSTMTHTMQ 348 >L35751 [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase Length = 533 Score = 182 bits (463), Expect = 5e-46, Method: Compositional matrix adjust. Identities = 15/368 (4%), Positives = 46/368 (12%), Gaps = 25/368 (6%) Query: 2 GLALLLTPLVKKFAVKIGATDVPNARKVHTKIMPRXXXXXXXXXXXXXXXXXXPIIPYDF 61 + ++ + ++ + ++ R P D Sbjct: 23 IGSNGMSFWAFRDDLQRLNQVEQSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADD 82 Query: 62 TPREA------------------NFIKXXXXXXXXXXXXXXXDDRFELSAKVKLLGQIAA 103 R+ + + + Sbjct: 83 IKTLMTTARASLTQSTTLFKSFMAMTAGNEHVRGLQKETEKSFARWHNDLEHQATWLESN 142 Query: 104 ACIVVFGFGITVDFVNIPFNNSYSSLESWIAIPLTIFWIVGVTNAVNLIDGLDGLAAGVS 163 + N LE + L+ + A + Sbjct: 143 QLSDFLTAPVQGSQNAFDVNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIY 202 Query: 164 GIAIATIAVMAFLM---GNTMVAXXXXXXXXXXXXXXXXXXHPAKIFMGDTGSXXXXXXX 220 + + IF Sbjct: 203 ISSALWWTRKMIVQPLAIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGT 262 Query: 221 XXXXXXXXKQIAVVSFITPLLIIGVPLSDTFFAIVRRKLQKKPIFAPDKGHLHHCLRELG 280 +++ + + A + + Sbjct: 263 VSDVRKGSQEMHIGIAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQA 322 Query: 281 FSHRQTVLIIYGIAGFFGILAVIQTSASLYEANWVTFVVICVMLFFLQIGAEITGVISKT 340 + G + I I + + Sbjct: 323 SELAKNAATTAQAGGVQVSTMTHTMQEI--ATSSQKIGDII--SVIDGIAFQTNILALNA 378 Query: 341 KRPLIDFL 348 Sbjct: 379 AVEAARAG 386 >SA0702 [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase Length = 533 Score = 181 bits (460), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 10/351 (2%), Positives = 47/351 (13%), Gaps = 18/351 (5%) Query: 1 MGLALLLTPLVKKFAVKIGATDVPNARKVHTKIMPRXXXXXXXXXXXXXXXXXXPIIPYD 60 + L L+L +++ + + + + ++ Sbjct: 11 LFLILILCGILQIGSNGMSFWAFRDDLQRLNQVEQSNQQRAALAQTRAVMLQASTA---- 66 Query: 61 FTPREANFIKXXXXXXXXXXXXXXXDDRFELSAKVKLLGQIAAACIVVFGFGITVDFVNI 120 S + + Sbjct: 67 ----LNK-AGTLTALSYPADDIKTLMTTARASLTQSTTLFKSFMAMTAG-NEHVRGLQKE 120 Query: 121 PFNNSYSSLESWIAIPLTIFWIVGVTNAVNLIDGLDGLAAGVSGIAIATIAVMAFLMGNT 180 + + + A + + L + Sbjct: 121 TEKSFARW--HNDLEHQATWLESNQLSDFLTAPVQGSQNAFDVNFEAWQLEINHVLEAAS 178 Query: 181 MVAXXXXXXXXXXXXXXXXXXHPAKIFMGDTGSXXXXXXXXXXXXXXXKQIAVVSFITPL 240 + A Sbjct: 179 AQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPLAIIGSHFDSIAAGNLARPIA 238 Query: 241 LIIGVPLSDTFFAIVRRKLQKKPIFAPDKGHLHHCLRELGFSHRQTVLIIYGIAGFFGIL 300 + ++ F ++ + + + + + + Sbjct: 239 VYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIGIAEIVAGNNDLSSRTE-----Q 293 Query: 301 AVIQTSASLYEANWVTFVVICVMLFF-LQIGAEITGVISKTKRPLIDFLLR 350 + + +T V + + + Sbjct: 294 QAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQAGGVQVSTMT 344 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.143 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,649,407 Number of extensions: 730027 Number of successful extensions: 2937 Number of sequences better than 1.0e-05: 57 Number of HSP's gapped: 2817 Number of HSP's successfully gapped: 58 Length of query: 363 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 255 Effective length of database: 25,922,359 Effective search space: 6610201545 Effective search space used: 6610201545 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 116 (49.3 bits)