BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0327.0005.p (271 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3737 [N] COG4786 Flagellar basal body rod protein 137 1e-32 TM1542 [N] COG4786 Flagellar basal body rod protein 125 6e-29 STM1542 [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 125 6e-29 BS_flhP [N] COG4786 Flagellar basal body rod protein 124 2e-28 BH2449 [N] COG4786 Flagellar basal body rod protein 121 1e-27 >BH3737 [N] COG4786 Flagellar basal body rod protein Length = 280 Score = 137 bits (346), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 91/279 (32%), Positives = 140/279 (50%), Gaps = 9/279 (3%) Query: 1 MNNSTIGAAVSMSSLQQRLDIIADNVANMNTDGYKSKQGSFEDVL-TRVQQQSKDYDQPG 59 MN S I A+V+M LQ++LD ++ N+AN NT GYK ++ SF D+L +V + G Sbjct: 1 MNLSMISASVTMGELQKKLDTLSHNIANSNTTGYKRREASFSDLLFQQVNTNKATQYETG 60 Query: 60 RSTPLGFNIGFGVRVPTVTTNWEEGPLKETGNPTDLAIQG-NGLFGVQV----NGATAYT 114 R TP G +G G +V E+G L T D AI + F ++ N T T Sbjct: 61 RLTPNGIRVGSGAKVSQTALRMEQGTLTTTDRALDFAIANPSSFFEIETVVDGNPVTRLT 120 Query: 115 RQGDFHFVPDSANPDRMMLVDNTGNPVLNTQGNPLTVPADVSAAIDENGRVLTKTAENAP 174 R G F+ + N + + LV G+ +L++ GN + +PA + L T N Sbjct: 121 RDGAFYLTANPNNDNLLQLVTQNGDYLLDSAGNRINIPATFEEITLTDQGTLQVTMPNGA 180 Query: 175 ARVAGTIMIVEPLSKSALQAVDGNFFVLADGVT---AQQAFKGAGEPSTIGVRSGYLEQS 231 A + +++ + L+AV GN F L D A+ A V G LE S Sbjct: 181 NVQAAQLQLIQVMKPQLLEAVGGNMFQLPDLAALGFAENEVYAAVGAGGGAVVQGTLEGS 240 Query: 232 NVDLSKEIAEMMQIQRTYQLAARALTSSDQMLGLANNMR 270 NVD+ +E+ ++++ QR YQ AR+++ +DQM+GL N +R Sbjct: 241 NVDIGREMTDLIETQRNYQFNARSVSMADQMMGLVNGLR 279 >TM1542 [N] COG4786 Flagellar basal body rod protein Length = 261 Score = 125 bits (315), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 90/279 (32%), Positives = 150/279 (53%), Gaps = 28/279 (10%) Query: 1 MNNSTIGAAVSMSSLQQRLDIIADNVANMNTDGYKSKQGSFEDVLTRVQQQSKDYDQPGR 60 M S AA MS+ Q +LD IA+N+AN++T GYK + F+D+L + + + Sbjct: 1 MMISLYSAATGMSAQQFKLDTIANNLANVDTTGYKKVRAEFQDLLYQYVKNAGTPTAATS 60 Query: 61 STPLGFNIGFGVRVPTVTTNWEEGPLKETGNPTDLAIQGNGLFGVQV-NGATAYTRQGDF 119 S P G +G GVR T + G ++TGN DLAI G+G F +Q+ +G AYTR G F Sbjct: 61 SLPTGLYVGHGVRTAATTRIFTLGNFEQTGNALDLAIAGDGFFQIQLQDGRIAYTRDGSF 120 Query: 120 HFVPDSANPDRMMLVDNTGNPVLNTQGNPLTVPAD-VSAAIDENGRVLTKTAENAPARVA 178 DS R++ T N +L +T+P + VS + +G +++ ++ + Sbjct: 121 KM--DSEG--RIV----TSNGLLLVP--EITIPENAVSINVSPDG-IVSAELQDGTIQEL 169 Query: 179 GTIMIVEPLSKSALQAVDGNFFVLA-------DGVTAQQAFKGAGEPSTIGVRSGYLEQS 231 GTI +V ++ S L+++ N ++ +GV Q F GA ++ G+LE+S Sbjct: 170 GTITLVRFVNPSGLKSIGDNLYIATPASGDPIEGVPGQDGF-GA-------IKQGFLEKS 221 Query: 232 NVDLSKEIAEMMQIQRTYQLAARALTSSDQMLGLANNMR 270 NVD+ +E+ +M+ QR Y+ +R + ++D+ML A N++ Sbjct: 222 NVDVVREMVDMITAQRAYEFNSRVIQTADEMLRTATNVK 260 >STM1542 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Length = 341 Score = 125 bits (315), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 12/279 (4%), Positives = 44/279 (15%), Gaps = 28/279 (10%) Query: 1 MNNSTIGAAVSMSSLQQRLDIIADNVANMNTDGYKSKQGSFEDVLTRVQQQSKDYDQPGR 60 M + + +D + + + R Y + Sbjct: 1 MKVKAVFINKPGEVETRWVDYPHKKENEVLIKVDAAGICGSDIGAFRGTNPLVTYPRIIG 60 Query: 61 STPLGFNIGFGVRVPTVTTNWEEGPLKETGNPTDLAIQGNGLFGVQV-NGATAYTRQGDF 119 G + G +P + + G G Sbjct: 61 HEVTGIVLQEGAGMPDNIKEGDRVIVDPYIYCGHCYPCSVGRTNCCENLNVIGVHVDGAM 120 Query: 120 HFVPDSANPDRMMLVDNTGNPVLNTQGNPLTVPAD-VSAAIDENGRVLTKTAENAPARVA 178 + + + + P + I + + + Sbjct: 121 QE--VVTH--PAH----LIHKIPDNV--PSEMAPLAEPLTIALHA-LHRANVKAGEYVAI 169 Query: 179 GTIMIVEPLSKSALQAVDGNFFVLA-------DGVTAQQAFKGAGEPSTIGVRSGYLEQS 231 + ++ + + ++ + + Sbjct: 170 IGAGAIGLMAALSARHYKATPILIDIVEERLRYAQKLGVPY-VL-------NAADDQVID 221 Query: 232 NVDLSKEIAEMMQIQRTYQLAARALTSSDQMLGLANNMR 270 + + + + D Sbjct: 222 AIKNITHGTMAQVVIEASGANSAIRNTLDLASFAGRISF 260 >BS_flhP [N] COG4786 Flagellar basal body rod protein Length = 268 Score = 124 bits (311), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 86/274 (31%), Positives = 154/274 (56%), Gaps = 16/274 (5%) Query: 4 STIGAAVSMSSLQQRLDIIADNVANMNTDGYKSKQGSFEDVLTR----VQQQSKDYDQPG 59 S + A+ +++ LQQ++D I+ N++N NT GYK+K +F +++ + V +++++ + Sbjct: 4 SMLTASTTLNQLQQQIDTISSNLSNSNTTGYKAKDTNFSELVRQQFDQVDEKNEEVAKAR 63 Query: 60 RSTPLGFNIGFGVRVPTVTTNWEEGPLKETGNPTDLAIQG-NGLFGVQVNGATAYTRQGD 118 ++ PL +G + V+ ++G +++T D+A V VNG YTR G Sbjct: 64 KTPPLAPLVGAMMSSRLVS---DQGSIQKTDRDLDIAFTSPYQYLQVNVNGNRQYTRDGA 120 Query: 119 FHFVPDSANPDRMMLVDNTGNPVLNTQGNPLTVPADV-SAAIDENGRVLTKTAENAPARV 177 + P +AN +++ LV G PVL+ GN + + + + + I++NG LT + NA R Sbjct: 121 LYVTPSAANANQLQLVTGNGYPVLDENGNTVNIDSSMKNITINKNG-TLTASDGNAVQRF 179 Query: 178 -AGTIMIVEPLSKSALQAVDGNFFVLADGVTAQQAFKGAGEPSTIGVRSGYLEQSNVDLS 236 G + + P L++ N F + D A + GA + IG++ G LE SNVD+S Sbjct: 180 NLGVVQVNNP---QELKSEGNNLFSI-DNAAAFEELNGANRQN-IGMQQGSLEMSNVDIS 234 Query: 237 KEIAEMMQIQRTYQLAARALTSSDQMLGLANNMR 270 +++ +++ QR+YQL +R +T DQMLGL N++R Sbjct: 235 EQMTDLITSQRSYQLNSRTITMGDQMLGLINSVR 268 >BH2449 [N] COG4786 Flagellar basal body rod protein Length = 263 Score = 121 bits (304), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 86/256 (33%), Positives = 136/256 (53%), Gaps = 16/256 (6%) Query: 12 MSSLQQRLDIIADNVANMNTDGYKSKQGSFEDVLTRVQQQSKDYDQPGRSTPLGFN---I 68 M + Q +LD+I +N+AN+NT GYK + +F+D+ V QQ PG + G N + Sbjct: 12 MRNFQTKLDVIGNNIANVNTFGYKKGRTTFQDM---VSQQLAGATAPGANRG-GVNPRQV 67 Query: 69 GFGVRVPTVTTNWEEGPLKETGNPTDLAIQGNGLFGVQVNGATAYTRQGDFHFVPDSANP 128 G G+++ ++ +G L+ T DL I G+G F VQ YTR G+F+F Sbjct: 68 GLGMQLASIDNVHTQGSLQNTNRELDLGISGDGFFQVQDGDTFYYTRSGNFYF------D 121 Query: 129 DRMMLVDNTGNPVLNTQGNPLTVPADV-SAAIDENGRVLTKTAENAPARVAGTIMIVEPL 187 + +V+ G +++T GNP+TVP S +I ++G V T E+ + AG + +V Sbjct: 122 QQGTIVNAQGLRLMDTNGNPITVPTTAQSFSIGQDGTV-TYIDEDGDLQEAGMVRLVNFA 180 Query: 188 SKSALQAVDGNFFVLADGVTAQQAFKGAGEPSTIGVRSGYLEQSNVDLSKEIAEMMQIQR 247 + L N ++++ A F AG T + +G LE SNVDLS+E EM+ QR Sbjct: 181 NPEGLAKAGANLYLVSANSGAP-LFTSAGNGGTGDIIAGTLEMSNVDLSEEFTEMIVAQR 239 Query: 248 TYQLAARALTSSDQML 263 +Q R +T+SD++L Sbjct: 240 GFQANTRIITTSDEIL 255 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.129 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,795,468 Number of extensions: 852918 Number of successful extensions: 1464 Number of sequences better than 1.0e-05: 41 Number of HSP's gapped: 1391 Number of HSP's successfully gapped: 46 Length of query: 271 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 166 Effective length of database: 26,268,361 Effective search space: 4360547926 Effective search space used: 4360547926 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 115 (48.9 bits)