BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0327.0002.p (247 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_spoIIQ [M] COG0739 Membrane proteins related to metalloendope... 157 2e-38 BH3746 [M] COG0739 Membrane proteins related to metalloendopepti... 142 4e-34 RC1093 [M] COG0739 Membrane proteins related to metalloendopepti... 60 3e-09 TP0864 [M] COG0739 Membrane proteins related to metalloendopepti... 57 2e-08 AGc3364 [M] COG0739 Membrane proteins related to metalloendopept... 53 5e-07 >BS_spoIIQ [M] COG0739 Membrane proteins related to metalloendopeptidases Length = 283 Score = 157 bits (397), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 86/215 (40%), Positives = 124/215 (57%), Gaps = 11/215 (5%) Query: 31 RMLSKRWVFPAVYTAAAAIILTLVWVYQDAGQKPLKPDTAAVVSQXXXXXXXXXXSAKDD 90 + KRWVFPA+Y +AA+ILT V YQ +K A SA D+ Sbjct: 16 QFFRKRWVFPAIYLVSAAVILTAVLWYQSVSNDEVKDQLA----------DNGGNSAYDN 65 Query: 91 -PAALEVVASAESMVWPVASPADVEVVKPFYDEKGTEENHVAAMVQYNDTFVPNAGIDIA 149 A+EV S E++ PV +V VVK FY+ +E AA+V YN+T+ + GID+A Sbjct: 66 NDDAVEVGKSMENVAMPVVDSENVSVVKKFYETDAAKEEKEAALVTYNNTYSLSKGIDLA 125 Query: 150 REDNQPFDVKAALSGEVTRVEDVPVFGKVVEITSTGNVKTVYQSLGDTKVKQGDEVKQGD 209 +D + FDV A+LSG V + E PV G VVE+ + TVYQSL + V+QGD+VKQ Sbjct: 126 EKDGKDFDVSASLSGTVVKAEKDPVLGYVVEVEHADGLSTVYQSLSEVSVEQGDKVKQNQ 185 Query: 210 MLATAGRSEIEKNLGNHVHFEVYEDGKLVNPSDLL 244 ++ +G++ ++ GNHVHFE+ +DG +NP + + Sbjct: 186 VIGKSGKNLYSEDSGNHVHFEIRKDGVAMNPLNFM 220 >BH3746 [M] COG0739 Membrane proteins related to metalloendopeptidases Length = 284 Score = 142 bits (358), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 87/231 (37%), Positives = 126/231 (54%), Gaps = 5/231 (2%) Query: 16 KNKQGDSGAKPSSWNRMLSKRWVFPAVYTAAAAIILTLVWVYQDAGQKPLKPDTAAVVSQ 75 +NKQ + + R L KRW PA+Y A AA++L+ + Q + PD V + Sbjct: 4 ENKQSSKKEETFNLQRFLKKRWALPAIYLAVAALLLSAFFFMQSQNGQIANPDDPIDVEE 63 Query: 76 XXXXXXXXXXSAKDDPAALEVVASAESMVWPVASPADVEVVKPFYDEKGTEENHVAAMVQ 135 + A+ V E + P++ +VE+V FYD GT E AA+V Sbjct: 64 NPNGP-----TGMHGEDAVPVTTDGEVVKLPISDENEVEIVGTFYDVNGTAEEQQAALVY 118 Query: 136 YNDTFVPNAGIDIAREDNQPFDVKAALSGEVTRVEDVPVFGKVVEITSTGNVKTVYQSLG 195 YN+ + N GID+A++ + FDV AALSG V + E + G VVE+ + T Y SLG Sbjct: 119 YNNYYYQNKGIDLAKDGEESFDVTAALSGTVVKAEQDSLLGYVVEVDHDNGIVTHYHSLG 178 Query: 196 DTKVKQGDEVKQGDMLATAGRSEIEKNLGNHVHFEVYEDGKLVNPSDLLPQ 246 + V+QGD V+QGD+L +AGR+ +N G HVHFE+ +DG +NP D+L Q Sbjct: 179 ELNVEQGDTVRQGDILGSAGRNLYNENAGVHVHFEIRKDGIAINPEDVLQQ 229 >RC1093 [M] COG0739 Membrane proteins related to metalloendopeptidases Length = 284 Score = 60.1 bits (144), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 33/102 (32%), Positives = 52/102 (50%), Gaps = 4/102 (3%) Query: 143 NAGIDIAREDNQPFDVKAALSGEVTRVEDVPVFGKVVEITSTGNVKTVYQSLGDTKVKQG 202 ++GID+ + P + AA SG V + P +G VEI T Y L + VK+G Sbjct: 181 HSGIDLQAKKAAP--IYAAASGIVIKAARAPDYGNFVEIKHGRKFVTKYAHLKEMSVKEG 238 Query: 203 DEVKQGDMLATAGRSEIEKNLGNHVHFEVYEDGKLVNPSDLL 244 +++K+G + G + G H+HFE+ D K +NP D + Sbjct: 239 NKIKRGQFIGIQGST--GNATGEHLHFEILLDNKAINPFDFI 278 >TP0864 [M] COG0739 Membrane proteins related to metalloendopeptidases Length = 546 Score = 57.4 bits (137), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 34/102 (33%), Positives = 52/102 (50%), Gaps = 8/102 (7%) Query: 145 GIDIAREDNQPF--DVKAALSGEVTRVEDVPVFGKVVEITSTGNVKTVYQSLGDTKVKQG 202 GID+A P+ VKA L G+V + ++G + I G +T+Y L V +G Sbjct: 449 GIDLA----APYGTQVKATLDGKVAEIGYSRIYGNYLIIVHGGGYQTMYGHLSSVMVGRG 504 Query: 203 DEVKQGDMLATAGRSEIEKNLGNHVHFEVYEDGKLVNPSDLL 244 V QG + G S + G H+HF V+++G +VNP +L Sbjct: 505 ARVVQGTTIGRVGAS--GRATGPHLHFSVFKNGSVVNPLKIL 544 >AGc3364 [M] COG0739 Membrane proteins related to metalloendopeptidases Length = 289 Score = 52.8 bits (125), Expect = 5e-07, Method: Compositional matrix adjust. Identities = 0/102 (0%), Positives = 0/102 (0%), Gaps = 4/102 (3%) Query: 143 NAGIDIAREDNQPFDVKAALSGEVTRVEDVPVFGKVVEITSTGNVKTVYQSLGDTKVKQG 202 Sbjct: 324 -- 381 Query: 203 DEVKQGDMLATAGRSEIEKNLGNHVHFEVYEDGKLVNPSDLL 244 Sbjct: 382 -- 421 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.310 0.128 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,243,617 Number of extensions: 698950 Number of successful extensions: 1469 Number of sequences better than 1.0e-05: 27 Number of HSP's gapped: 1515 Number of HSP's successfully gapped: 27 Length of query: 247 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 143 Effective length of database: 26,383,695 Effective search space: 3772868385 Effective search space used: 3772868385 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 114 (48.5 bits)