BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0320.0004.p (484 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1405 [G] COG2723 Beta-glucosidase/6-phospho-beta-glucosidase/... 716 0.0 lin0288 [G] COG2723 Beta-glucosidase/6-phospho-beta-glucosidase/... 570 e-162 BS_bglH [G] COG2723 Beta-glucosidase/6-phospho-beta-glucosidase/... 569 e-162 lin0017 [G] COG2723 Beta-glucosidase/6-phospho-beta-glucosidase/... 556 e-158 CAC1408 [G] COG2723 Beta-glucosidase/6-phospho-beta-glucosidase/... 543 e-154 >CAC1405 [G] COG2723 Beta-glucosidase/6-phospho-beta-glucosidase/beta- galactosidase Length = 473 Score = 716 bits (1847), Expect = 0.0, Method: Compositional matrix adjust. Identities = 342/476 (71%), Positives = 392/476 (82%), Gaps = 10/476 (2%) Query: 11 GFLWGGATAANQFEGGYKEGGKGLSTADVITAGTHTTPRRITPVLEQGMNYPSHEAIDFY 70 GFLWGGATAANQFEG + E GKG STAD++T GTHTTPRRITPVLE+G YPSHEAIDFY Sbjct: 6 GFLWGGATAANQFEGAWNEDGKGPSTADMMTGGTHTTPRRITPVLEEGTYYPSHEAIDFY 65 Query: 71 HHYKEDIQLFAEMGFKVFRMSIAWSRIFPNGDDAQPNEEGLRFYDRVFAELKKHNIEPLV 130 HHYKEDI+LFAEMGFK FR+SIAWSRIFPNGDD +PNE GL+FYD VF EL K+NIEPLV Sbjct: 66 HHYKEDIKLFAEMGFKTFRLSIAWSRIFPNGDDKEPNEAGLQFYDNVFDELLKYNIEPLV 125 Query: 131 TISHYEAPFRLAQQYNGWSDRRVVDFYVNYCDVIFNRYKDVVKYWLTFNEINILTMP-FG 189 TISHYE PF L ++YNGW R V+DFY YC+ IFNRYKD VKYWLTFNEIN LT+P FG Sbjct: 126 TISHYETPFGLTEKYNGWVGREVIDFYTRYCETIFNRYKDKVKYWLTFNEINCLTIPQFG 185 Query: 190 SFMAGAILPEGSAEFGNTAGDNE-QLRYQALHHQFIASARAVKLGHSINKDFKIGCMIAY 248 ++M G I+P+ GD+E QLR+QALHHQF+ASA+AVKLGH IN +FKIGCMIAY Sbjct: 186 AYMGGGIIPK--------EGDSEAQLRFQALHHQFVASAKAVKLGHEINPNFKIGCMIAY 237 Query: 249 MCSYPLTCSPEDVTLAQQKDNLTNFLCSDVQVRGAYPGFAKRYFKEKGIELVIEEGDDEI 308 MC+Y TC+P+DV Q+KD + N LCSDVQVRG YPGFA YFKE GIE+ +E+ D++I Sbjct: 238 MCNYAKTCNPDDVLETQRKDQIHNMLCSDVQVRGYYPGFALNYFKENGIEIKMEKDDEKI 297 Query: 309 LREGRVDFXXXXXXXXTCVSADPDREKVGGNLSMGLKNPYLEASAWEWQIDPKGLRWSLN 368 LREG VDF VS D + ++V GNL G KNPYL++S W WQIDPKGLRW+LN Sbjct: 298 LREGCVDFYSFSYYMSNLVSTDKNDKEVSGNLLGGYKNPYLKSSDWGWQIDPKGLRWTLN 357 Query: 369 NIYNRYEIPLMVVENGLGAVDTVEADGSINDDYRIDYLRRHIAEMKEAVADGVELIGYTP 428 +IY+RY+IP+MVVENGLGAVD +E DGSINDDYRIDYLR HI EM+EAV +GV+L+GYT Sbjct: 358 HIYDRYQIPVMVVENGLGAVDVIEEDGSINDDYRIDYLRDHIVEMEEAVKNGVDLMGYTM 417 Query: 429 WGCIDLVSAGTGEMKKRYGFIYVDKDNDGQGTLARSRKKSFSWYKKVIASNGEVLE 484 WGCIDLVSA TGEMKKRYGFIYVDKDNDG GT+AR KKSF+WYKKVIASNGE LE Sbjct: 418 WGCIDLVSASTGEMKKRYGFIYVDKDNDGNGTMARKPKKSFNWYKKVIASNGENLE 473 >lin0288 [G] COG2723 Beta-glucosidase/6-phospho-beta-glucosidase/beta- galactosidase Length = 486 Score = 570 bits (1468), Expect = e-162, Method: Compositional matrix adjust. Identities = 275/496 (55%), Positives = 348/496 (70%), Gaps = 22/496 (4%) Query: 1 MNELTKNFPQGFLWGGATAANQFEGGYKEGGKGLSTADVIT----------AGTHTTPRR 50 M E+ K FP+GFLWGGATAANQ EG Y GKGLSTAD++ + Sbjct: 1 MTEVKKQFPKGFLWGGATAANQVEGAYDVDGKGLSTADMVKFIPKEERTKDHALDVSKAE 60 Query: 51 ITPVLEQGMN--YPSHEAIDFYHHYKEDIQLFAEMGFKVFRMSIAWSRIFPNGDDAQPNE 108 I ++ + +P E +DFYH YKEDI LFAEMGFK FR+S+ W+RIFPNGDD +PNE Sbjct: 61 IEAIIAGKVEGRFPKREGVDFYHRYKEDIALFAEMGFKTFRLSLNWARIFPNGDDKEPNE 120 Query: 109 EGLRFYDRVFAELKKHNIEPLVTISHYEAPFRLAQQYNGWSDRRVVDFYVNYCDVIFNRY 168 +GL FYD+VF EL K++IEPLVT+SHYE P L +YNGW+DRRV+ F+ NY + +F RY Sbjct: 121 KGLEFYDKVFDELLKYDIEPLVTLSHYETPLNLTLKYNGWADRRVIGFFTNYAETVFKRY 180 Query: 169 KDVVKYWLTFNEINILTMPFGSFMAGAILPEGSAEFGNTAGDNEQLRYQALHHQFIASAR 228 K+ VKYWLTFNEIN++++ ++ G +L E A + +L YQA HHQF+ASA Sbjct: 181 KNKVKYWLTFNEINVISL--SAYTGGGVLLE-------DAKNPLELSYQAGHHQFVASAL 231 Query: 229 AVKLGHSINKDFKIGCMIAYMCSYPLTCSPEDVTLAQQKDNLTNFLCSDVQVRGAYPGFA 288 A KL H I ++GCM+A M +YP T +P+D+ L Q +N N +DV RG YP + Sbjct: 232 ATKLAHEIIPGSQVGCMLARMATYPATNNPDDI-LKAQYENQQNLFFTDVHARGEYPSYM 290 Query: 289 KRYFKEKGIELVIEEGDDEILREGRVDFXXXXXXXXTCVSADPDREKVGGNLSMGLKNPY 348 R+F+E I +V E GDDEIL+ VDF +A P+ ++ GNL G+KN Y Sbjct: 291 NRFFQENDINIVKEVGDDEILKAHTVDFISFSYYMSLSATASPEGDRSAGNLMGGVKNEY 350 Query: 349 LEASAWEWQIDPKGLRWSLNNIYNRYEIPLMVVENGLGAVDTVEADGSINDDYRIDYLRR 408 LE+S W WQIDPKGLRW+LN++Y+RYE+PL +VENGLGA DTVE DG I+DDYRIDYLR+ Sbjct: 351 LESSDWGWQIDPKGLRWTLNDLYSRYELPLFIVENGLGAYDTVEEDGKIHDDYRIDYLRK 410 Query: 409 HIAEMKEAVADGVELIGYTPWGCIDLVSAGTGEMKKRYGFIYVDKDNDGQGTLARSRKKS 468 HI +MKEA+ADGV+L+GYT WG IDLVSA T EM KRYGFIYVD+D+ G+GTL RSRK S Sbjct: 411 HIEQMKEAIADGVDLMGYTSWGPIDLVSASTSEMSKRYGFIYVDQDDWGKGTLERSRKDS 470 Query: 469 FSWYKKVIASNGEVLE 484 F WYKKVI +NGE L+ Sbjct: 471 FFWYKKVIETNGEDLD 486 >BS_bglH [G] COG2723 Beta-glucosidase/6-phospho-beta-glucosidase/beta- galactosidase Length = 481 Score = 569 bits (1467), Expect = e-162, Method: Compositional matrix adjust. Identities = 284/487 (58%), Positives = 340/487 (69%), Gaps = 22/487 (4%) Query: 1 MNELTKNFPQGFLWGGATAANQFEGGYKEGGKGLSTADVITAGTHTTPRRITPVLEQGMN 60 M+ K FP+GFLWGGA AANQ EG Y EGGKGLSTADV +P I ++ M Sbjct: 13 MSSNEKRFPEGFLWGGAVAANQVEGAYNEGGKGLSTADV-------SPNGIMSPFDESMT 65 Query: 61 YPS--HEAIDFYHHYKEDIQLFAEMGFKVFRMSIAWSRIFPNGDDAQPNEEGLRFYDRVF 118 + H IDFYH YKEDI LFAEMGFK FR SIAW+RIFPNGD+ +PNEEGLRFYD +F Sbjct: 66 SLNLYHNGIDFYHRYKEDIALFAEMGFKAFRTSIAWTRIFPNGDEEEPNEEGLRFYDDLF 125 Query: 119 AELKKHNIEPLVTISHYEAPFRLAQQYNGWSDRRVVDFYVNYCDVIFNRYKDVVKYWLTF 178 EL KH+IEP+VTISHYE P L + Y GW +R+V++FY Y +F RY+ VKYW+TF Sbjct: 126 DELLKHHIEPVVTISHYEMPLGLVKNYGGWKNRKVIEFYERYAKTVFKRYQHKVKYWMTF 185 Query: 179 NEINI-LTMPFGSFMAGAILPEGSAEFGNTAGDNEQLRYQALHHQFIASARAVKLGHSIN 237 NEIN+ L PF G + EG + YQA HHQF+ASA AVK GH I Sbjct: 186 NEINVVLHAPFTG--GGLVFEEGENKL--------NAMYQAAHHQFVASALAVKAGHDII 235 Query: 238 KDFKIGCMIAYMCSYPLTCSPEDVTLAQQKDNLTNFLCSDVQVRGAYPGFAKRYFKEKGI 297 D KIGCMIA +YP+T PEDV A + + T F SDVQ RGAYPG+ KRY E I Sbjct: 236 PDSKIGCMIAATTTYPMTSKPEDVFAAMENERKTLFF-SDVQARGAYPGYMKRYLAENNI 294 Query: 298 ELVIEEGDDEILREGRVDFXXXXXXXXTCVSADPDR-EKVGGNLSMGLKNPYLEASAWEW 356 E+ + EGD+E+L+E VD+ S DP+ K GGNL G+KNPYL++S W W Sbjct: 295 EIEMAEGDEELLKEHTVDYIGFSYYMSMAASTDPEELAKSGGNLLGGVKNPYLKSSEWGW 354 Query: 357 QIDPKGLRWSLNNIYNRYEIPLMVVENGLGAVDTVEADGSINDDYRIDYLRRHIAEMKEA 416 QIDPKGLR +LN +Y+RY+ PL +VENGLGAVD VE DG+I DDYRI+YLR H+ E +EA Sbjct: 355 QIDPKGLRITLNTLYDRYQKPLFIVENGLGAVDKVEEDGTIQDDYRINYLRDHLIEAREA 414 Query: 417 VADGVELIGYTPWGCIDLVSAGTGEMKKRYGFIYVDKDNDGQGTLARSRKKSFSWYKKVI 476 +ADGVELIGYT WG IDLVSA T EMKKRYGFIYVD+DN+G GT R +KKSF+WY++VI Sbjct: 415 IADGVELIGYTSWGPIDLVSASTAEMKKRYGFIYVDRDNEGNGTFNRIKKKSFNWYQQVI 474 Query: 477 ASNGEVL 483 A+NGE L Sbjct: 475 ATNGESL 481 >lin0017 [G] COG2723 Beta-glucosidase/6-phospho-beta-glucosidase/beta- galactosidase Length = 477 Score = 556 bits (1433), Expect = e-158, Method: Compositional matrix adjust. Identities = 274/483 (56%), Positives = 344/483 (71%), Gaps = 17/483 (3%) Query: 8 FPQGFLWGGATAANQFEGGYKEGGKGLSTADVI-TAGTHTTPRRITPVLEQGMNY---PS 63 FP+GFLWGGA AANQ EG Y E GKGLS D++ T P +Y PS Sbjct: 6 FPKGFLWGGAVAANQCEGAYLEDGKGLSLVDILPTVEDGRWEALFNPSKALATDYGFYPS 65 Query: 64 HEAIDFYHHYKEDIQLFAEMGFKVFRMSIAWSRIFPNGDDAQPNEEGLRFYDRVFAELKK 123 HE+IDFYH YKEDI+L AEMGFK FRMSI+W RIFPNGD+ PNE+GL FYD VF E K Sbjct: 66 HESIDFYHRYKEDIKLMAEMGFKCFRMSISWPRIFPNGDETTPNEKGLAFYDAVFDECHK 125 Query: 124 HNIEPLVTISHYEAPFRLAQQYNGWSDRRVVDFYVNYCDVIFNRYKDVVKYWLTFNEIN- 182 + IEP+VTI+H++ P + ++Y GW +R+ +DFY+N+C+ IF RYKD VKYW+TFNEIN Sbjct: 126 YGIEPVVTINHFDTPLEVFKKYGGWKNRKCIDFYLNFCEAIFTRYKDKVKYWMTFNEINM 185 Query: 183 ILTMPFGSFMAGAILPEGSAEFGNTAGDNEQLRYQALHHQFIASARAVKLGHSINKDFKI 242 IL +P+ + G + A + E+++YQA HHQ +ASA A KLGH IN + +I Sbjct: 186 ILHLPY---IGGGLDVTKEA-------NPEEVKYQAAHHQLVASALATKLGHEINPENQI 235 Query: 243 GCMIAYMCSYPLTCSPEDVTLAQQKDNLTNFLCSDVQVRGAYPGFAKRYFKEKGIELVIE 302 GCM+A +YP+TC+P+DV + + D F DVQ RG YP + KR+FKE I + +E Sbjct: 236 GCMLAAGNTYPMTCNPKDVWKSIEADREGYFFI-DVQARGYYPSYTKRFFKEHNINIKME 294 Query: 303 EGDDEILREGRVDFXXXXXXXXTCVSADPDREK-VGGNLSMGLKNPYLEASAWEWQIDPK 361 +GD + LR+ VD+ SADP++ K GN+ LKNPYL+AS W WQIDP Sbjct: 295 DGDLDALRDHTVDYVAFSYYSSRLTSADPEKNKETEGNVFATLKNPYLKASEWGWQIDPL 354 Query: 362 GLRWSLNNIYNRYEIPLMVVENGLGAVDTVEADGSINDDYRIDYLRRHIAEMKEAVADGV 421 GLR ++N IY+RY+ PL +VENGLGAVDTVE DGSINDDYRIDY+R H+ EM EA+ DGV Sbjct: 355 GLRITMNTIYDRYQKPLFIVENGLGAVDTVEEDGSINDDYRIDYMREHVREMGEAIEDGV 414 Query: 422 ELIGYTPWGCIDLVSAGTGEMKKRYGFIYVDKDNDGQGTLARSRKKSFSWYKKVIASNGE 481 EL+GYTPWGCIDLVSAG+GEMKKRYGFIYVD+DN G GTL RS+KKSF WYKKVI +NG+ Sbjct: 415 ELLGYTPWGCIDLVSAGSGEMKKRYGFIYVDRDNKGNGTLNRSKKKSFDWYKKVIETNGK 474 Query: 482 VLE 484 ++ Sbjct: 475 DID 477 >CAC1408 [G] COG2723 Beta-glucosidase/6-phospho-beta-glucosidase/beta- galactosidase Length = 477 Score = 543 bits (1400), Expect = e-154, Method: Compositional matrix adjust. Identities = 279/488 (57%), Positives = 337/488 (69%), Gaps = 18/488 (3%) Query: 4 LTKNFPQGFLWGGATAANQFEGGYKEGGKGLSTADVITAGTHTTPRRITPV----LEQGM 59 ++K F + FLWGGATAANQ EG Y E KGLST DVI G P + + + Sbjct: 1 MSKGFSKEFLWGGATAANQCEGAYLEDNKGLSTVDVIPKGKDRFPVGLGKMKMLECDSEH 60 Query: 60 NYPSHEAIDFYHHYKEDIQLFAEMGFKVFRMSIAWSRIFPNGDDAQPNEEGLRFYDRVFA 119 YPSHEAIDFYH YKEDI LF EMGFK FR+S+AWSRIFPNGDD PNEEGL+FYD VF Sbjct: 61 YYPSHEAIDFYHRYKEDIALFHEMGFKCFRLSLAWSRIFPNGDDEIPNEEGLKFYDAVFD 120 Query: 120 ELKKHNIEPLVTISHYEAPFRLAQQYNGWSDRRVVDFYVNYCDVIFNRYKDVVKYWLTFN 179 E K+ IEPLVTI+H++ P L + W ++V++Y C VIF+RYK+ VKYWLTFN Sbjct: 121 ECLKYGIEPLVTITHFDVPVNLVKTVGSWRSSKMVEYYEKLCKVIFSRYKNKVKYWLTFN 180 Query: 180 EINILT-MPFGSFMAGAILPEGSAEFGNTAGDNEQLRYQALHHQFIASARAVKLGHSINK 238 EIN+L +PF AG + EG E E ++YQA HHQ IASA+A K+ IN Sbjct: 181 EINMLLHLPF--IGAGLVFEEGENE--------EAIKYQAAHHQLIASAKATKIAREINP 230 Query: 239 DFKIGCMIAYMCSYPLTCSPEDVTLAQQKDNLTNFLCSDVQVRGAYPGFAKRYFKEKGIE 298 +FKIGCM+A +Y TC+PEDV + +KD N+ DVQ RG YP +AK+ K KGIE Sbjct: 231 NFKIGCMLAAGNNYANTCAPEDVWRSIEKDR-ENYFFIDVQSRGEYPNYAKKMLKRKGIE 289 Query: 299 LVIEEGDDEILREGRVDFXXXXXXXXTCVSADPD-REKVGGNLSMGLKNPYLEASAWEWQ 357 L +E+ D IL+ VDF SA+P+ GN+ LKNPYL+AS W WQ Sbjct: 290 LQVEDEDAGILKNNTVDFISFSYYSSRLTSANPNINGNTEGNVFATLKNPYLKASEWGWQ 349 Query: 358 IDPKGLRWSLNNIYNRYEIPLMVVENGLGAVDTVEADGSINDDYRIDYLRRHIAEMKEAV 417 IDP GLR +LN++Y+RY+ PL +VENGLGAVDT + +G + DDYRI+YLR HI M++AV Sbjct: 350 IDPLGLRITLNSLYDRYQKPLFIVENGLGAVDTPDENGYVEDDYRIEYLREHIKAMRDAV 409 Query: 418 -ADGVELIGYTPWGCIDLVSAGTGEMKKRYGFIYVDKDNDGQGTLARSRKKSFSWYKKVI 476 DGVEL+GYTPWGCIDLVSA TGEMKKRYGFIYVDKDNDG GTL RS+KKSF WYKKVI Sbjct: 410 NVDGVELMGYTPWGCIDLVSASTGEMKKRYGFIYVDKDNDGNGTLKRSKKKSFYWYKKVI 469 Query: 477 ASNGEVLE 484 SNG +E Sbjct: 470 ESNGMDIE 477 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,866,162 Number of extensions: 1712594 Number of successful extensions: 2765 Number of sequences better than 1.0e-05: 54 Number of HSP's gapped: 2515 Number of HSP's successfully gapped: 62 Length of query: 484 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 374 Effective length of database: 25,691,691 Effective search space: 9608692434 Effective search space used: 9608692434 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)