BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0315.0009.p (216 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_glnP [E] COG0765 ABC-type amino acid transport system, permea... 280 9e-76 SP0607 [E] COG0765 ABC-type amino acid transport system, permeas... 149 2e-36 DR2277 [E] COG0765 ABC-type amino acid transport system, permeas... 148 6e-36 BS_glnM [E] COG0765 ABC-type amino acid transport system, permea... 146 3e-35 mlr3799 [E] COG0765 ABC-type amino acid transport system, permea... 143 2e-34 >BS_glnP [E] COG0765 ABC-type amino acid transport system, permease component Length = 219 Score = 280 bits (717), Expect = 9e-76, Method: Compositional matrix adjust. Identities = 27/216 (12%), Positives = 65/216 (30%), Gaps = 2/216 (0%) Query: 1 LDFAGAFSADNLKFLLDGLYVTLIVAFVSIILSFVIGCVIGVVRYAGIPVLSPVMFYLVE 60 + F + + L++G +TL ++ + + VIG + G R G + + V +E Sbjct: 1 MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFARTFGGWIANHVALVFIE 60 Query: 61 LIRNLPLLLIIFFVRFALPEVGIKMSLITAAIVALTIFEAAMIAEIVRGGLTSIDKGQIE 120 +IR P+++ + F+ FALP + + +TI + + + Sbjct: 61 VIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSGAYIAEITRGAVLSIHKGF 120 Query: 121 AARSSGLSSFQTLWHIVLPQGLRRMVPPLVSQFISLLKDTSLAVVISLPELMHNANIVIG 180 L + + L ++ ++ I + Sbjct: 121 REAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIKDTSLFIVIGVAELTRQGQEI 180 Query: 181 HSY--SYAIPTLMLVALIYFAVNYMLSVVSRKLENK 214 + + + ++ R Sbjct: 181 IAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRM 216 >SP0607 [E] COG0765 ABC-type amino acid transport system, permease component Length = 219 Score = 149 bits (377), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 85/210 (40%), Positives = 129/210 (61%), Gaps = 8/210 (3%) Query: 8 SADNLKFLLDGLYVTLIVAFVSIILSFVIGCVIGVVRYAGIPVLSPVMFYLVELIRNLPL 67 + DNL F+ G +TL ++ ++IILS +IG V+ V R PVL + +E +RN+P Sbjct: 9 TPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVTRNGKNPVLRIISSIYIEFVRNVPN 68 Query: 68 LLIIFFVRFALPEVGIKMSLITAAIVALTIFEAAMIAEIVRGGLTSIDKGQIEAARSSGL 127 LL IF + KM A I A T+F +A +AEI+RGGL ++DKGQ EA S G Sbjct: 69 LLWIFTIFLVF-----KMKSTPAGITAFTLFTSAALAEIIRGGLNAVDKGQYEAGMSQGF 123 Query: 128 SSFQTLWHIVLPQGLRRMVPPLVSQFISLLKDTSLAV-VISLPELMHNANIVIGHSY--S 184 +S Q L++I+LPQ +R+M+P ++SQF++++KDTSL VI+L EL + I++G + Sbjct: 124 TSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLYSVIALQELFGASQILMGRYFEPE 183 Query: 185 YAIPTLMLVALIYFAVNYMLSVVSRKLENK 214 +L+ALIYF+ N +S +S L + Sbjct: 184 QVFSLYILIALIYFSFNLAISSLSHMLAKR 213 >DR2277 [E] COG0765 ABC-type amino acid transport system, permease component Length = 239 Score = 148 bits (374), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 84/217 (38%), Positives = 133/217 (61%), Gaps = 9/217 (4%) Query: 2 DFAGAFSADNLKFLLDGLYVTLIVAFVSIILSFVIGCVIGVVRYAGIPVLSPVMFYLVEL 61 F A S D + LL GL VTL V+ ++++S V+G +G+VR +P+L P+ VE+ Sbjct: 15 GFQTALSGDYPQLLLSGLGVTLAVSLCALVVSVVVGTALGLVRVYRVPLLGPIGNIYVEV 74 Query: 62 IRNLPLLLIIFFVRFALPEVGIKMSLITAAIVALTIFEAAMIAEIVRGGLTSIDKGQIEA 121 +R +PL++++ + F LP +G+ ++ AA++AL ++ AA +EIVRGGL S+ GQIEA Sbjct: 75 VRGIPLIVLLSVIYFGLPTLGLTLAGFPAAVLALGLYSAAYTSEIVRGGLGSVPAGQIEA 134 Query: 122 ARSSGLSSFQTLWHIVLPQGLRRMVPPLVSQFISLLKDTSLAVVISLPELMHNANIVIGH 181 ARS GLS Q L +VLPQ R +P L ++F+SL+ +SLA ++L EL + Sbjct: 135 ARSLGLSRGQALRFVVLPQAWRVALPALGNEFVSLILGSSLASAVTLQELFSQGRYITNA 194 Query: 182 SYS----YAIPTLMLVALIYFAVNYMLSVVSRKLENK 214 +Y YA+ +A+IYF + + L+ R LE++ Sbjct: 195 TYRQFEVYAV-----LAVIYFVLTFTLTRAVRALEHR 226 >BS_glnM [E] COG0765 ABC-type amino acid transport system, permease component Length = 216 Score = 146 bits (368), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 82/208 (39%), Positives = 127/208 (61%), Gaps = 7/208 (3%) Query: 10 DNLKFLLDGLYVTLIVAFVSIILSFVIGCVIGVVRYAGIPVLSPVMFY---LVELIRNLP 66 DN +DG Y TL+ + +++ SFV+G + V+R I V P+ + VE IRN+P Sbjct: 8 DNFSMYMDGFYHTLLASVIALAGSFVLGVAVAVMR---ITVFKPLQWLGTAYVEFIRNIP 64 Query: 67 LLLIIFFVRFALPEVGIKMSLITAAIVALTIFEAAMIAEIVRGGLTSIDKGQIEAARSSG 126 LLLI F F LP G+++ A VALTI+ +A IAE +R G+ S+ KGQ+EAARSSG Sbjct: 65 LLLITFVFYFGLPNAGLRLDGFQAGTVALTIYTSAFIAEAIRAGIQSVSKGQMEAARSSG 124 Query: 127 LSSFQTLWHIVLPQGLRRMVPPLVSQFISLLKDTSLAVVISLPELMHNANIVIGHSYSYA 186 + Q + HI+LPQ ++ ++PPL +QF++L+K++S+ V++ +LM+ A++V S Sbjct: 125 FTYSQAMLHIILPQAIKIVIPPLGNQFLNLVKNSSILGVVAGLDLMYQADLV-SSSTLVV 183 Query: 187 IPTLMLVALIYFAVNYMLSVVSRKLENK 214 + VAL Y + LS+ LE + Sbjct: 184 FDVYIFVALFYLVLTIPLSIGVNYLEKR 211 >mlr3799 [E] COG0765 ABC-type amino acid transport system, permease component Length = 216 Score = 143 bits (361), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 82/205 (40%), Positives = 125/205 (60%), Gaps = 1/205 (0%) Query: 12 LKFLLDGLYVTLIVAFVSIILSFVIGCVIGVVRYAGIPVLSPVMFYLVELIRNLPLLLII 71 L FL G VTL V+ + + LS V G ++GV G L + + IR +P+L+++ Sbjct: 9 LPFLAKGFAVTLWVSLLVVTLSLVAGVLLGVGLVYGPAPLRWAVRIFSDTIRGIPILVLM 68 Query: 72 FFVRFALPEVGIKMSLITAAIVALTIFEAAMIAEIVRGGLTSIDKGQIEAARSSGLSSFQ 131 FFV + LP +G+ + AA++ALT+F+ A + E +RG + SI KGQ EAA + GL+ Q Sbjct: 69 FFVYYGLPAIGLHLPSFWAAVLALTLFKTAQVIEYLRGAVASIPKGQSEAAMAIGLTFRQ 128 Query: 132 TLWHIVLPQGLRRMVPPLVSQFISLLKDTSLAVVISLPELMHNANIVIGHSYSYAIPTLM 191 L ++ PQ RR +PP ++ +K ++L ++ + +LM N VIG +Y A+P + Sbjct: 129 RLTFVIFPQAFRRFLPPWINGVTDAVKGSALVSLLGITDLMQAINQVIGRTYE-AMPLYI 187 Query: 192 LVALIYFAVNYMLSVVSRKLENKTA 216 L ALIYFAVNY LS+ SR+LE + A Sbjct: 188 LGALIYFAVNYALSLASRRLERRFA 212 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.330 0.144 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,574,510 Number of extensions: 482657 Number of successful extensions: 2815 Number of sequences better than 1.0e-05: 214 Number of HSP's gapped: 2736 Number of HSP's successfully gapped: 214 Length of query: 216 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 114 Effective length of database: 26,614,363 Effective search space: 3034037382 Effective search space used: 3034037382 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 113 (48.1 bits)