BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0315.0006.p (319 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yfjQ [P] COG0598 Mg2+ and Co2+ transporters 330 2e-90 SA2137 [P] COG0598 Mg2+ and Co2+ transporters 170 3e-42 STM0561 [T] COG0642 Signal transduction histidine kinase 169 6e-42 sll0671 [P] COG0598 Mg2+ and Co2+ transporters 162 9e-40 sll0507 [P] COG0598 Mg2+ and Co2+ transporters 160 2e-39 >BS_yfjQ [P] COG0598 Mg2+ and Co2+ transporters Length = 273 Score = 330 bits (847), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 0/319 (0%), Positives = 0/319 (0%), Gaps = 3/319 (0%) Query: 1 MIRTLAVTHTGEVLTDLPLQDITIADYAWVWADFSTPTAEETLLLDTYFHFHPLAIEDCM 60 M R + ++ + L Sbjct: 1 MTRLASRFGAANLIRRDRPLTREELFRVVPSVFSEDKHESRSERYTYIPTISLLDSLQRE 60 Query: 61 HILQRPKLDYYEDVQFFVLHALNERTLEAEEIDLFLSKRFLVSYHHQEKQEMEEAWQMVK 120 H + L E ++ + + + + Sbjct: 61 GFQPFFACQTRVRDPRRREHTKHMLRLRREGQITGKQVPEIILLNSHDGTSSYQMLPGMF 120 Query: 121 AEIPSRKGWSGGPMAAAYTVMDKLVDRYFPSLYSLEDELADLESQGGSESVEELMSQVFN 180 + + V + E+ + + + + Sbjct: 121 R---AVCQNGLVCGESFGEVRVPHKGDVVSQVIEGAYEVLGIFERVEEKRDAMQSLLLPP 177 Query: 181 VRGRLLKLRRTIVPMRDLMYRILNSQHVQSNGEERIYFGDIYDHLLKLTDMIEVDREMTA 240 + L + + SQ + + + ++I+ Sbjct: 178 PVQQALAKAALTYRFGEDHQPVTESQILSPRRWQDESNDLWTTYQRIQENLIKGGLSGRN 237 Query: 241 DLRDSYISLNSNRMNSIMKTLTVITTIFMPLTLIAGIYGMNFRVMPELDWKYGYFXXXXX 300 Sbjct: 238 297 Query: 301 XXXXXXXXXRWFRRSGWFK 319 Sbjct: 298 316 >SA2137 [P] COG0598 Mg2+ and Co2+ transporters Length = 315 Score = 170 bits (431), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 91/294 (30%), Positives = 155/294 (52%), Gaps = 5/294 (1%) Query: 26 DYAWVWADFSTPTAEETLLLDTYFHFHPLAIEDCMHILQRPKLDYYEDVQFFVLHALNER 85 D VW DF P +E +F+F+ L ++D ++ + R K Y+D Q+ V H++ Sbjct: 26 DATIVWYDFDEPNEQENEWFKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGS 85 Query: 86 TLEAEEIDLFLSKRFLVSYHHQEKQEMEEAWQMVKAEIPSRKGWSGGPMAAAYTVMDKLV 145 +++F+ LV+YHHQ + + + + + + ++D +V Sbjct: 86 NYSPIALNIFIQDNVLVTYHHQTLESLNKVVYKYMNTLDAELDCAD----VVILILDMMV 141 Query: 146 DRYFPSLYSLEDELADLESQGGSESVEEL-MSQVFNVRGRLLKLRRTIVPMRDLMYRILN 204 D+YF +Y+LED + E + + ++ M VF +R L+K++R + PM++L+ + Sbjct: 142 DKYFNFVYALEDSVYHFEDRHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQ 201 Query: 205 SQHVQSNGEERIYFGDIYDHLLKLTDMIEVDREMTADLRDSYISLNSNRMNSIMKTLTVI 264 + + + + +Y I DHL+K ++I +EMT ++R+++ S S RMNSIM+ LT++ Sbjct: 202 NGDLIIDNKHSLYIQHIDDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLV 261 Query: 265 TTIFMPLTLIAGIYGMNFRVMPELDWKYGYFXXXXXXXXXXXXXXRWFRRSGWF 318 + IF PLT IAGIYGMNF MP L YGY+ +FRR WF Sbjct: 262 SVIFSPLTFIAGIYGMNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF 315 >STM0561 [T] COG0642 Signal transduction histidine kinase Length = 109 Score = 169 bits (428), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 0/315 (0%), Positives = 0/315 (0%), Gaps = 13/315 (4%) Query: 10 TGEVLTDLPLQDITIADYAWVWADFSTPTAEETLLLDTYFHFHPLAIEDCMHILQRPKLD 69 L D + + R Sbjct: 44 PIAPEHLPLLFDRFYRVDPSRQRKGEGSGIGL---AIVKSIVTAHRGKISVTSDTRATRF 100 Query: 70 YYEDVQFFVLHAL----NERTLEAEEIDLFLSKRFLVSYHHQEKQEMEEAWQMVKAEIPS 125 Sbjct: 101 ---- 156 Query: 126 RKGWSGGPMA--AAYTVMDKLVDRYFPSLYSLEDELADLESQGGSESVEELMSQVFNVRG 183 Sbjct: 157 216 Query: 184 RLLKLRRTIVPMRDLMYRILNSQHVQSNGEERIYFGDIYDHLLKLTDMIEVDREMTADLR 243 Sbjct: 217 276 Query: 244 DSYISLNSNRMNSIMKTLTVITTIFMPLTLIAGIYGMNFRVMPELDWKYGYFXXXXXXXX 303 Sbjct: 277 336 Query: 304 XXXXXXRWFRRSGWF 318 Sbjct: 337 351 >sll0671 [P] COG0598 Mg2+ and Co2+ transporters Length = 380 Score = 162 bits (409), Expect = 9e-40, Method: Compositional matrix adjust. Identities = 97/287 (33%), Positives = 157/287 (54%), Gaps = 15/287 (5%) Query: 45 LDTYFHFHPLAIEDCMHILQRPKLDYYEDVQFFVLHAL----NERTLEAEEIDLFLSKRF 100 L F HP+A+ED +++ QRPK+ YE+ F+ + + +T +E+I L K + Sbjct: 87 LGEVFKLHPVALEDIVNVPQRPKVVEYENHLIFISRMVTLDQSSQTFISEQISFILGKHY 146 Query: 101 LVSYHHQEKQEMEEAWQMVKAEIPSRKGWSGGPMA--AAYTVMDKLVDRYFPSLYSLEDE 158 L++ + K + + V+ I ++KG A Y ++D ++D +FP + + Sbjct: 147 LLTIQEEPKYDCLFS---VRERIRTKKGAIRQKNADYLFYALIDAIIDGFFPVMEVYGEL 203 Query: 159 LADLESQGGSESVEELMSQVFNVRGRLLKLRRTIVPMRDLMYRILNSQHVQSNGEERIYF 218 + L+S+ S + ++++ ++ LL +RR I P RD + +L + E R++ Sbjct: 204 VQSLQSEIISCPTNKSLAKIHQLQQDLLIMRRAIWPQRDAINSLLRDGSDLISDEVRVFL 263 Query: 219 GDIYDHLLKLTDMIEVDREMTADLRDSYISLNSNRMNSIMKTLTVITTIFMPLTLIAGIY 278 D YDH +++ DMIE R++ ++L D Y+S SNRMN IMKTLTVI++IF+PLT IAGIY Sbjct: 264 RDCYDHTIQILDMIETYRDLASNLTDIYLSSVSNRMNEIMKTLTVISSIFIPLTFIAGIY 323 Query: 279 GMNFRV------MPELDWKYGYFXXXXXXXXXXXXXXRWFRRSGWFK 319 GMNF MPEL+W +GY +F R GWF+ Sbjct: 324 GMNFNPDKSPWNMPELNWYWGYPVIWMVMLTVGGMMLYFFWRKGWFR 370 >sll0507 [P] COG0598 Mg2+ and Co2+ transporters Length = 387 Score = 160 bits (405), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 100/297 (33%), Positives = 164/297 (55%), Gaps = 11/297 (3%) Query: 31 WADFSTPTAEETLL-LDTYFHFHPLAIEDCMHILQRPKLDYYEDVQFFVLHAL----NER 85 W D +EE L + F HPL +ED +++ QR K++ Y D + H + E Sbjct: 86 WMDIEGLGSEEVLKEVGEIFKLHPLLLEDIVNVPQRAKVEDYNDHVMVIAHRVRPNREED 145 Query: 86 TLEAEEIDLFLSKRFLVSYHHQEKQEMEEAWQMVKAEIPSRKG--WSGGPMAAAYTVMDK 143 E+E++ L KR+L+++ ++ + + + ++ I + +G G Y ++D Sbjct: 146 GFESEQVSFVLGKRYLLTF---QEGHIIDCFNPLRERIRTNQGKVCQQGADYLCYLLIDM 202 Query: 144 LVDRYFPSLYSLEDELADLESQGGSESVEELMSQVFNVRGRLLKLRRTIVPMRDLMYRIL 203 L+D YFP L E+ + LE LM +++++R LL LRR I P+R +M +L Sbjct: 203 LIDEYFPLLEDYEERIEALEDTIIRNPNSSLMEEIYHIRRELLALRRLIWPLRHVMNVLL 262 Query: 204 -NSQHVQSNGEERIYFGDIYDHLLKLTDMIEVDREMTADLRDSYISLNSNRMNSIMKTLT 262 ++ + + RIYF D YDH++++ D+IE RE+ + L + Y++ SN+MN +MK LT Sbjct: 263 RDTTNSIVTADVRIYFRDCYDHIIQVLDIIEAYRELASSLMEVYMTAMSNKMNEVMKFLT 322 Query: 263 VITTIFMPLTLIAGIYGMNFRVMPELDWKYGYFXXXXXXXXXXXXXXRWFRRSGWFK 319 VI+TIF+PLT IAG+YGMNF+ MPEL+ ++GY+ +F R GW Sbjct: 323 VISTIFIPLTFIAGVYGMNFKEMPELNSRWGYYITWIVMLLIAGGSLYFFWRKGWLS 379 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.138 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,637,383 Number of extensions: 890631 Number of successful extensions: 4292 Number of sequences better than 1.0e-05: 40 Number of HSP's gapped: 4261 Number of HSP's successfully gapped: 40 Length of query: 319 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 213 Effective length of database: 26,153,027 Effective search space: 5570594751 Effective search space used: 5570594751 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 116 (49.3 bits)