BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0315.0002.p (281 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1865 [IR] COG1597 Sphingosine kinase and enzymes related to e... 266 2e-71 BS_yerQ [IR] COG1597 Sphingosine kinase and enzymes related to e... 261 9e-70 BH0676 [IR] COG1597 Sphingosine kinase and enzymes related to eu... 260 2e-69 SA1714 [IR] COG1597 Sphingosine kinase and enzymes related to eu... 225 7e-59 L26628 [IR] COG1597 Sphingosine kinase and enzymes related to eu... 196 4e-50 >lin1865 [IR] COG1597 Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase Length = 310 Score = 266 bits (680), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 135/279 (48%), Positives = 183/279 (65%), Gaps = 1/279 (0%) Query: 1 MKKRLADILDRLDIGGIEATCHXXXXXXXXXXXXXXXXXXXCYDLIIAAGGDGTLNEVIN 60 +KK LAD+L L+ G + H YDL++AAGGDGT+NEVIN Sbjct: 18 IKKNLADVLSILEQAGYVTSAHATTAEPGDAKHAAEEAVRDRYDLVVAAGGDGTINEVIN 77 Query: 61 GMAEKXXXXXXXXXXXXXXXDFARAMGIPKNWEDSCDLILRQESRLIDLGKANDRYFINI 120 G+AE+ DFARA+ +P++ + +I +S +D+GKAND YFINI Sbjct: 78 GIAEQPYRPKVGIIPTGTTNDFARALHVPRDVIKATKIIAAGQSVAMDIGKANDTYFINI 137 Query: 121 AGGGRLTELTYEVPSRLKTMMGQLAYYLKGIEKMASLSPQELYIRANGQELIHDEFMLFL 180 GGGRLTELTY+VPSRLKTM+GQLAYYLKGIE + SL ++ + + Q + E M FL Sbjct: 138 GGGGRLTELTYDVPSRLKTMLGQLAYYLKGIEMLPSLKATKVKVEYD-QGVFEGEVMFFL 196 Query: 181 IANTNSVGGFEKLAPDARIDDGLFDVIGVKKCNLAEFIRLVSLAIRGEHLQDKKVVYFRT 240 + TNS+GGFEK+APDA++DDG F +I VKK NLAEFIRLV+LA+RG+H+++ V+Y ++ Sbjct: 197 LGLTNSIGGFEKIAPDAKLDDGKFSLIIVKKVNLAEFIRLVTLALRGDHIKEPNVIYVKS 256 Query: 241 DAMEVTSPGYVQLNLDGELGGTLPGQFSILPQHLQIFAQ 279 + + V S + +NLDGELGG P +F L QH++ FA Sbjct: 257 EKVSVHSEDKMLINLDGELGGETPMEFRNLKQHIEFFAS 295 >BS_yerQ [IR] COG1597 Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase Length = 303 Score = 261 bits (667), Expect = 9e-70, Method: Compositional matrix adjust. Identities = 133/276 (48%), Positives = 184/276 (66%), Gaps = 3/276 (1%) Query: 2 KKRLADILDRLDIGGIEATCHXXXXXXXXXXXXXXXXXXXCYDLIIAAGGDGTLNEVING 61 KK LA +L + + G E + H +DLIIAAGGDGT+NEV+NG Sbjct: 18 KKHLAQVLQKFEQAGYETSTHATTCAGDATHAAKEAALRE-FDLIIAAGGDGTINEVVNG 76 Query: 62 MAEKXXXXXXXXXXXXXXXDFARAMGIPK-NWEDSCDLILRQESRLIDLGKANDRYFINI 120 +A DFARA+GIP+ + + D ++ +R ID+G+ N +YFINI Sbjct: 77 LAPLDNRPTLGVIPVGTTNDFARALGIPREDILKAADTVINGVARPIDIGQVNGQYFINI 136 Query: 121 AGGGRLTELTYEVPSRLKTMMGQLAYYLKGIEKMASLSPQELYIRANGQELIHDEFMLFL 180 AGGGRLTELTY+VPS+LKTM+GQLAYYLKG+E + SL P E+ I +G+ L E MLFL Sbjct: 137 AGGGRLTELTYDVPSKLKTMLGQLAYYLKGMEMLPSLRPTEVEIEYDGK-LFQGEIMLFL 195 Query: 181 IANTNSVGGFEKLAPDARIDDGLFDVIGVKKCNLAEFIRLVSLAIRGEHLQDKKVVYFRT 240 + TNSVGGFEKLAPD+ ++DG+FD++ +KK NLAEFIR+ ++A+RGEH+ D+ ++Y + Sbjct: 196 VTLTNSVGGFEKLAPDSSLNDGMFDLMILKKANLAEFIRVATMALRGEHINDQHIIYTKA 255 Query: 241 DAMEVTSPGYVQLNLDGELGGTLPGQFSILPQHLQI 276 + ++V +QLNLDGE GG LPG+F L +H+ + Sbjct: 256 NRVKVNVSEKMQLNLDGEYGGMLPGEFVNLYRHIHV 291 >BH0676 [IR] COG1597 Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase Length = 295 Score = 260 bits (665), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 129/277 (46%), Positives = 183/277 (66%), Gaps = 2/277 (0%) Query: 1 MKKRLADILDRLDIGGIEATCHXXXXXXXXXXXXXXXXXXXCYDLIIAAGGDGTLNEVIN 60 ++K LA IL+RL++ G E + H +DL+IAAGGDGT+ EV+N Sbjct: 17 IRKSLAYILERLELAGYETSTHATTGEGSATEAARLAGERR-HDLVIAAGGDGTIYEVVN 75 Query: 61 GMAEKXXXXXXXXXXXXXXXDFARAMGIPKNWEDSCDLILRQESRLIDLGKANDRYFINI 120 G+A DFARA+ IP++ E +CD+++ ++ +D+GKA D YFINI Sbjct: 76 GLAGLEKRPTLGLIPAGTTNDFARALNIPRDIEKACDILIEGHTKPVDVGKAGDHYFINI 135 Query: 121 AGGGRLTELTYEVPSRLKTMMGQLAYYLKGIEKMASLSPQELYIRANGQELIHDEFMLFL 180 A GG LTELTYEVPS+LKT++GQLAYY+KG+EK+ +SP ++ I +G+ L E M+FL Sbjct: 136 AAGGTLTELTYEVPSKLKTVVGQLAYYIKGLEKLPQISPTDVRIEYDGK-LFEGEIMMFL 194 Query: 181 IANTNSVGGFEKLAPDARIDDGLFDVIGVKKCNLAEFIRLVSLAIRGEHLQDKKVVYFRT 240 ++NTNSVGGFE+LAP+A + DG+FD I VKK + EF+ L LA+RGEH++ KV+Y + Sbjct: 195 VSNTNSVGGFERLAPNASLRDGMFDFIIVKKTSFPEFLHLAGLALRGEHIKHPKVLYVQA 254 Query: 241 DAMEVTSPGYVQLNLDGELGGTLPGQFSILPQHLQIF 277 + ++V + +QLN DGE GG LP +F L QH Q+ Sbjct: 255 NRIKVQAEKSMQLNFDGEYGGLLPAEFVNLYQHFQML 291 >SA1714 [IR] COG1597 Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase Length = 315 Score = 225 bits (573), Expect = 7e-59, Method: Compositional matrix adjust. Identities = 117/281 (41%), Positives = 171/281 (60%), Gaps = 2/281 (0%) Query: 1 MKKRLADILDRLDIGGIEATCHXXXXXXXXXXXXXXXXXXXCYDLIIAAGGDGTLNEVIN 60 K+ L D L +L+ G E + + YD++IAAGGDGTLNEV+N Sbjct: 18 FKRELPDALIKLEKAGYETSAYATEKIGDATLEAERAMHE-NYDVLIAAGGDGTLNEVVN 76 Query: 61 GMAEKXXXXXXXXXXXXXXXDFARAMGIPKNWEDSCDLILRQESRLIDLGKANDRYFINI 120 G+AEK DF RA+ IP + + D+I+ S +D+GK N+RYFIN+ Sbjct: 77 GIAEKPNRPKLGVIPMGTVNDFGRALHIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINL 136 Query: 121 AGGGRLTELTYEVPSRLKTMMGQLAYYLKGIEKMASLSPQELYIRANGQELIHDEFMLFL 180 A GG+LT+++YE PS+LK+++G AYY+KG E + + +L I +G + E +LF Sbjct: 137 AAGGQLTQVSYETPSKLKSIVGPFAYYIKGFEMLPQMKAVDLRIEYDGN-VFQGEALLFF 195 Query: 181 IANTNSVGGFEKLAPDARIDDGLFDVIGVKKCNLAEFIRLVSLAIRGEHLQDKKVVYFRT 240 + TNS+ GFEKL PDA++DDG F +I V+K NLAE +++LA RGEH + KV+Y + Sbjct: 196 LGLTNSMAGFEKLVPDAKLDDGYFTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKA 255 Query: 241 DAMEVTSPGYVQLNLDGELGGTLPGQFSILPQHLQIFAQNN 281 A+ ++S +QLN+DGE GG LP F L +H+ +FA N+ Sbjct: 256 KAINISSFTDLQLNVDGEYGGKLPANFLNLERHIDVFAPND 296 >L26628 [IR] COG1597 Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase Length = 311 Score = 196 bits (498), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 11/292 (3%), Positives = 35/292 (11%), Gaps = 22/292 (7%) Query: 1 MKKRLADILDRLDIGGIEATCHXXXXXXXXXXXXXXXXXXXCYDLIIAAGGDGTLNEVIN 60 + + + + I Sbjct: 17 KRNGWMTVAAVSSVTITLVLVGLFLSVILNTSKISNGIENNVRIVAYMKLDVHDQETKIQ 76 Query: 61 GMAEKXXXXXXXXXXXXXXXDFARAMGIPKNWE-DSCDLILRQESRLIDLGKA------N 113 + + L ++ + Sbjct: 77 DPKDNKKTINNPDHLKIYDSIKKVPNVKTVEFSSKEAQLKELTKALGNNWNLFKGDANPL 136 Query: 114 DRYFINIAGGGRLTELTYEVPSRLKTMMGQLAYYLKGIEKMASLSPQELYIRANGQELIH 173 +I A + S+L + A L + Sbjct: 137 YDAYIVEADKPANVKSVTSKISKLSGIEKATYGGANT----AKLFQI---AQGIRTWGTA 189 Query: 174 DE------FMLFLIANTNSVGGFEKLAPDARIDDGLFDVIGVKKCNLAEFIRLVSLAIRG 227 + + + G + L + +I Sbjct: 190 AAILLIFVAVFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFFLEGAWVGLLGSIIP 249 Query: 228 EHLQDKKVVYFRTDAMEVTSPG--YVQLNLDGELGGTLPGQFSILPQHLQIF 277 + + + + Sbjct: 250 GLIVAWAYRLAFVSLTPSLQSQKLAMFAPKEFIPAIVGLMAVIGILIGSFGA 301 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.141 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,289,264 Number of extensions: 652362 Number of successful extensions: 972 Number of sequences better than 1.0e-05: 21 Number of HSP's gapped: 930 Number of HSP's successfully gapped: 21 Length of query: 281 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 176 Effective length of database: 26,268,361 Effective search space: 4623231536 Effective search space used: 4623231536 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)