BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0312.0008.p (322 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ybaS [R] COG0385 Predicted Na+-dependent transporter 251 1e-66 BH0489 [R] COG0385 Predicted Na+-dependent transporter 223 3e-58 BH0858 [R] COG0385 Predicted Na+-dependent transporter 86 8e-17 BS_yocS [R] COG0385 Predicted Na+-dependent transporter 77 4e-14 Cgl2276 [R] COG0385 Predicted Na+-dependent transporter 69 1e-11 >BS_ybaS [R] COG0385 Predicted Na+-dependent transporter Length = 310 Score = 251 bits (641), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 22/269 (8%), Positives = 55/269 (20%) Query: 40 WLFAFMTFAGSISLGFKDFVNVLKKPLPLFACLFILHIVMPLVALGIGNVLYHADEFTIT 99 + L L V + G +D Sbjct: 5 NKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVAIVTCDGNVYSAGDSDYRFAL 64 Query: 100 GLVLAAVIPTGVSSFIWVSIYKGNXXXXXXXXXXXXXXAPFVVPGVLSLLIGTSVELDTG 159 + + + A + G + + + T Sbjct: 65 ESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVIALELHGGKPLSPLVNAGAIATT 124 Query: 160 AMMSSLFWMIVVPSLLGMVLNEWTKGAVAPVWSPRLNPFSKLFMAAVIMINGSVISPYLA 219 +++++ +L + + + + A+ + S Y Sbjct: 125 SLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQSEQTTNFHNRAIAWLLYSAGYLYCD 184 Query: 220 DISLHLVGLAFVIVLLASTGYALCFLTARVLRWNEADEVALIFNGGMRNISAGAVLAVSY 279 + V LL + A T N ++ + I A ++ Y Sbjct: 185 AMEACDVYTRQCSTLLNTIELATLGATLAAGGVNPLTHKRVLQADNVPYILAEMMMEGLY 244 Query: 280 FPAPVAVPVVLGMVFQQLLASLAGFLLSH 308 + V + + + Sbjct: 245 GRSGDWAYRVGLPGKSGVGGGILAVVPGV 273 >BH0489 [R] COG0385 Predicted Na+-dependent transporter Length = 325 Score = 223 bits (569), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 122/299 (40%), Positives = 181/299 (60%), Gaps = 3/299 (1%) Query: 5 VNRTLNRMMPLITPLSVLTGVLCGSALSSYTYLSPWLFAFMTFAGSISLGFKDFVNVLKK 64 +NR L + MP++TP+SV GV S++ PWLFA ++F G++ L D L++ Sbjct: 12 INRVLEKGMPVLTPVSVAIGVWMAERFSNFASAVPWLFAVISFVGALGLTLVDIRERLRR 71 Query: 65 PLPLFACLFILHIVMPLVALGIGNVLYHADEFTITGLVLAAVIPTGVSSFIWVSIYKGNX 124 P + LFIL I+MPL+AL +G L+ D T +GLVLA IPTGV S +WV+IY GN Sbjct: 72 PQAMIVTLFILQILMPLIALLVGMWLF--DPMTRSGLVLAFSIPTGVMSLMWVTIYHGNK 129 Query: 125 XXXXXXXXXXXXXAPFVVPGVLSLLIGTSVELDTGAMMSSLFWMIVVPSLLGMVLNEWTK 184 +PF +P L IGT + D G M+ L WMIV+PSLL + +T Sbjct: 130 SFALAVVFIHTLLSPFFIPWTLRWFIGTDIPFDVGGMVWGLLWMIVIPSLLAISATRYTP 189 Query: 185 GAVAPVWSPRLNPFSKLFMAAVIMINGSVISPYLADISLHLVGLAFVIVLLASTGYALCF 244 V P+ L+P +KL + +IMIN SV +P++ L L+ + ++ +A +GY L + Sbjct: 190 -KVVPMMKDVLSPIAKLHLFLIIMINSSVAAPFIRSWDLSLLYIILTVLAIALSGYGLGY 248 Query: 245 LTARVLRWNEADEVALIFNGGMRNISAGAVLAVSYFPAPVAVPVVLGMVFQQLLASLAG 303 + AR+L+ +E+ +++++ GMRNI AGA LAV+YFP PV VP+V ++FQQ+LA+ G Sbjct: 249 VGARLLKLDESMRISMVYTCGMRNIGAGAALAVAYFPPPVMVPIVAAILFQQVLAAALG 307 >BH0858 [R] COG0385 Predicted Na+-dependent transporter Length = 323 Score = 85.9 bits (211), Expect = 8e-17, Method: Compositional matrix adjust. Identities = 76/258 (29%), Positives = 123/258 (47%), Gaps = 10/258 (3%) Query: 34 YTYLSPW---LFAFMTFAGSISLGFKDFVNVLKKPLPLFACLFILHIVMPLVALGIGNVL 90 +T+++P L + F ++L DF VL+KP+P+ + ++MPLVA + Sbjct: 33 FTWITPHITILLGVIMFGMGLTLKLSDFRIVLQKPIPVLVGVLAQFVIMPLVAFALAYAF 92 Query: 91 YHADEFTITGLVLAAVIPTGVSSFIWVSIYKGNXXXXXXXXXXXXXXAPFVVPGVLSLLI 150 E GLVL P G +S + V + KGN AP V P +L LL Sbjct: 93 NLPPELA-AGLVLVGACPGGTASNVMVYLAKGNVAASVAMTSVSTMLAPIVTPFILLLLA 151 Query: 151 GTSVELDTGAMMSSLFWMIVVPSLLGMVLNEWTKGAVAPVWSPRLNPFSKLFMAAVIMIN 210 G + +D AM S+ MI+VP LG+ + + AV S + P + A++ I Sbjct: 152 GQWLPIDAKAMFVSILQMIIVPIALGLFVRKMAPNAVDK--STAVLPLVSI--VAIMAIV 207 Query: 211 GSVISPYLADISLHLVGLAFVIVLLAST-GYALCFLTARVLRWNEADEVALIFNGGMRNI 269 +V+ A++ + L F+ V+L + G L +LTA+ + +E+ A+ GM+N Sbjct: 208 SAVVGANQANL-MSGAALLFLAVMLHNVFGLLLGYLTAKFVGLDESTRRAISIEVGMQNS 266 Query: 270 SAGAVLAVSYFPAPVAVP 287 GA LA ++F A+P Sbjct: 267 GLGAALAGNHFSPLAALP 284 >BS_yocS [R] COG0385 Predicted Na+-dependent transporter Length = 321 Score = 76.6 bits (187), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 66/292 (22%), Positives = 135/292 (46%), Gaps = 8/292 (2%) Query: 32 SSYTYLSPWLFAFM---TFAGSISLGFKDFVNVLKKPLPLFACLFILHIVMPLVALGIGN 88 S +T++S ++ F+ F ++L DF +++KP + + + +MPLVA G+ Sbjct: 31 SLFTWISSYITIFLGIIMFGMGLTLQADDFKELVRKPWQVIIGVIAQYTIMPLVAFGLAF 90 Query: 89 VLYHADEFTITGLVLAAVIPTGVSSFIWVSIYKGNXXXXXXXXXXXXXXAPFVVPGVLSL 148 L+ E + G++L P G +S + + KGN AP V P ++ L Sbjct: 91 GLHLPAEIAV-GVILVGCCPGGTASNVMTFLAKGNTALSVAVTTISTLLAPVVTPLLIML 149 Query: 149 LIGTSVELDTGAMMSSLFWMIVVPSLLGMVLNEWTKGAVAPVWSPRLNPFSKLFMAAVIM 208 + + G++ S+ ++ P + G+++ + + VA ++ + + ++ Sbjct: 150 FAKEWLPVSPGSLFISILQAVLFPIIAGLIVKMFFRKQVAKA----VHALPLVSVIGIVA 205 Query: 209 INGSVISPYLADISLHLVGLAFVIVLLASTGYALCFLTARVLRWNEADEVALIFNGGMRN 268 I +V+S ++ + + V++L GY L FL A++L+ + + A+ GM+N Sbjct: 206 IVSAVVSGNRENLLQSGLLIFSVVILHNGIGYLLGFLCAKLLKMDYPSQKAIAIEVGMQN 265 Query: 269 ISAGAVLAVSYFPAPVAVPVVLGMVFQQLLASLAGFLLSHRAKVRHTAEQTT 320 GA LA ++F AVP + V+ L S+ S + K + +++ Sbjct: 266 SGLGAALATAHFSPLSAVPSAIFSVWHNLSGSMLATYWSKKVKKKQAGSKSS 317 >Cgl2276 [R] COG0385 Predicted Na+-dependent transporter Length = 324 Score = 68.9 bits (167), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 66/294 (22%), Positives = 139/294 (47%), Gaps = 18/294 (6%) Query: 37 LSPWLFAFMT---FAGSISLGFKDFVNVLKKPLPLFACLFILHIVMPLVALGIGNVLYHA 93 L+ ++ F+T F ++L DF VLK+PLP+ + ++MP +A+ + +++ Sbjct: 38 LTNYINIFLTIIMFTMGLTLTVPDFQMVLKRPLPILIGVVAQFVIMPFLAIVVAK-MFNL 96 Query: 94 DEFTITGLVLAAVIPTGVSSFIWVSIYKGNXXXXXXXXXXXXXXAPFVVPGVLSLLIGTS 153 + GL++ +P G SS + + +G+ +P + P ++ +L GT Sbjct: 97 NPALAVGLLMLGSVPGGTSSNVIAFLARGDVALSVTMTSVSTIVSPIMTPFLMLMLAGTE 156 Query: 154 VELDTGAMMSSLFWMIVVPSLLGMV----LNEWTKGAVAPVWSPRLNPFSKLFMAAVIMI 209 +D G M +L +++P ++G+V LN+W + P L S L + V+ Sbjct: 157 TAVDGGGMAWTLVQTVLLPVIIGLVLRVFLNKWIDKIL-----PILPYLSILGIGGVVF- 210 Query: 210 NGSVISPYLADISLHLVGLAFVIVLLAST-GYALCFLTARVLRWNEADEVALIFNGGMRN 268 G+V + +S+ L+ FV V++ + GY + +LT RV ++ EA + G ++ Sbjct: 211 -GAVAANAERLVSVGLI--VFVAVIVHNVLGYVVGYLTGRVFKFPEAANRTMAIEIGTQS 267 Query: 269 ISAGAVLAVSYFPAPVAVPVVLGMVFQQLLASLAGFLLSHRAKVRHTAEQTTAA 322 + +A +F A+P + + + ++ L+ +R + + ++ + A Sbjct: 268 AGLASGMAGRFFTPEAALPGAVAALVHNITGAVYVGLVRNRPLTKASRKKESVA 321 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.140 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,441,779 Number of extensions: 816867 Number of successful extensions: 2814 Number of sequences better than 1.0e-05: 18 Number of HSP's gapped: 2792 Number of HSP's successfully gapped: 18 Length of query: 322 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 216 Effective length of database: 26,153,027 Effective search space: 5649053832 Effective search space used: 5649053832 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 116 (49.3 bits)