BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0311.0023.p (196 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yeeK [K] COG1309 Transcriptional regulator 99 3e-21 ECs1489 [K] COG1309 Transcriptional regulator 93 3e-19 STM1213 [K] COG1309 Transcriptional regulator 91 1e-18 mll1598 [K] COG1309 Transcriptional regulator 88 6e-18 SMa1299 [K] COG1309 Transcriptional regulator 77 1e-14 >BS_yeeK [K] COG1309 Transcriptional regulator Length = 378 Score = 99.4 bits (246), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 65/190 (34%), Positives = 92/190 (48%), Gaps = 2/190 (1%) Query: 3 RPREFDEEKALHAAMQIFWEKGYEATSLSDLTATMGIQRPSIYSAFGDKKGLFEAALRMY 62 R +EFD LH A+++F E GYE TSL DL + +GI R S+Y +G K+ LF +A++ Y Sbjct: 187 RNKEFDTALVLHRAIEVFGEYGYEGTSLQDLLSHLGIARQSLYDTYGTKRDLFLSAVKSY 246 Query: 63 TKSHAASVRASLHKKASVKAAFRAFFEHLVEEEYREEGSNKGCFCINTMVELSPHDAKFE 122 + A+V L SVK A R F+ V R+ K C+ IN+ +E PHD + Sbjct: 247 LEGKNAAVMERLEAPGSVKEAIRDIFQEGV-NALRDPERAKACYIINSAIEQIPHDPELA 305 Query: 123 ILTREHQMYLAVIFQETLERGIQSGELDS-GTDAKGLAHSLVIALIGLTVMMKCRPERNF 181 L F L R GEL+ TD LA L + + LT + K + + Sbjct: 306 RYFERQSKQLEDAFYHGLLRAKDQGELNGEDTDISALARYLNQSRLSLTFIAKVTADMDR 365 Query: 182 MDQAVYSMLS 191 + V LS Sbjct: 366 LQDHVDVSLS 375 >ECs1489 [K] COG1309 Transcriptional regulator Length = 236 Score = 92.8 bits (229), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 58/171 (33%), Positives = 88/171 (51%), Gaps = 4/171 (2%) Query: 3 RPREFDEEKALHAAMQIFWEKGYEATSLSDLTATMGIQRPSIYSAFGDKKGLFEAALRMY 62 RP+ FD + AL AM++FW+ GYEATSL+DL G + P++Y+ F +K+GLF A L Y Sbjct: 41 RPKVFDRDAALDKAMKLFWQHGYEATSLADLVEATGAKAPTLYAEFTNKEGLFRAVLDRY 100 Query: 63 TKSHAASVRASLH-KKASVKAAFRAFFEHLVEEEYREEGSNKGCFCINTMVELSPHDAKF 121 AA A L ++ SV++A +F + + + + GCF IN LSP Sbjct: 101 IDRFAAKHEAQLFCEEKSVESALADYFA-AIANCFTGKDTPAGCFMINNCTTLSPDSGDI 159 Query: 122 -EILTREHQMYLAVIFQETLERGIQSGELDSGTDAKGLAHSLVIALIGLTV 171 L H M + Q +R + GE+ + D LA L + G+++ Sbjct: 160 ANTLKSRHAMQERTLQQFLCQRQAR-GEIPTHCDVTHLAEFLNCIIQGMSI 209 >STM1213 [K] COG1309 Transcriptional regulator Length = 211 Score = 90.9 bits (224), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 59/172 (34%), Positives = 90/172 (52%), Gaps = 6/172 (3%) Query: 3 RPREFDEEKALHAAMQIFWEKGYEATSLSDLTATMGIQRPSIYSAFGDKKGLFEAALRMY 62 RP+ FD E AL AM +FW+ GYEATSL+DL G + P++Y+ F +KKGLF A L Y Sbjct: 15 RPKVFDREAALDKAMTLFWQHGYEATSLADLVEATGAKAPTLYAEFTNKKGLFRAVLDRY 74 Query: 63 TKSHAASVRASLH-KKASVKAAFRAFFEHLVEEEYREEGSNKGCFCINTMVEL--SPHDA 119 A+ A L ++ SV++A +F + + + GCF INT L S D Sbjct: 75 ITRFASKHEAQLFCEEKSVESALEDYFTEIA-ACFTSTDTPAGCFMINTSATLAASSRDI 133 Query: 120 KFEILTREHQMYLAVIFQETLERGIQSGELDSGTDAKGLAHSLVIALIGLTV 171 + +R H M + Q +R + GE+ + + + LA + L G+++ Sbjct: 134 ARTVKSR-HAMQEQTLIQFLRQRQ-ERGEIPAHCNPQALAEYINCILQGMSI 183 >mll1598 [K] COG1309 Transcriptional regulator Length = 209 Score = 88.2 bits (217), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 58/195 (29%), Positives = 84/195 (43%), Gaps = 4/195 (2%) Query: 3 RPREFDEEKALHAAMQIFWEKGYEATSLSDLTATMGIQRPSIYSAFGDKKGLFEAALRMY 62 RPR FD ++AL AAM +FWE GYEATSL+ L MG+ P IY+AF DK+ LF AL Y Sbjct: 11 RPRAFDPDRALDAAMHLFWEHGYEATSLAMLREAMGLTPPQIYNAFTDKETLFRKALTRY 70 Query: 63 TKSHAASVRASLHKKASVKAAFRAFFEHLVEEEYREEGSNKGCFCINTMVELSPHDAKFE 122 ++ +L A R E Y G GC ++ + SP Sbjct: 71 HETEIGFALDALSAPVPTPEAIRRLLLG-AAEAYSRPGKPGGCLFVSGALAASPRAQAIA 129 Query: 123 ILTREHQMYLAVIFQETLERGIQSGELDSGTDAKGLAHSLVIALIGLTVMMK---CRPER 179 + ++ E L G +G+L G + A + + G+++ + E Sbjct: 130 DELKAYRKASEAAIAERLAMGQAAGDLPEGFSVESFAKYIAGVMNGMSIQARDGASAEEL 189 Query: 180 NFMDQAVYSMLSSNG 194 M Q + L G Sbjct: 190 RIMAQTALAALPPEG 204 >SMa1299 [K] COG1309 Transcriptional regulator Length = 203 Score = 77.4 bits (189), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 56/180 (31%), Positives = 79/180 (43%), Gaps = 1/180 (0%) Query: 3 RPREFDEEKALHAAMQIFWEKGYEATSLSDLTATMGIQRPSIYSAFGDKKGLFEAALRMY 62 RPREFD +AL AA++IF EKGY TS+++L MG+ SIY AF DK+ +F AA Y Sbjct: 13 RPREFDINQALDAAIRIFSEKGYHGTSIAELKTEMGLTAGSIYKAFRDKRDVFVAAYDRY 72 Query: 63 TKSHAASVRASLHKKASVKAAFRAFFEHLVEEEYREEGSNKGCFCINTMVELSPHDAKFE 122 + A + +L ++ + E G +GC I VEL DA+ Sbjct: 73 KQIRADLLDQALFSASTGREKIARVVGFYAMSACGETG-RRGCLAIGAAVELVLSDAEIA 131 Query: 123 ILTREHQMYLAVIFQETLERGIQSGELDSGTDAKGLAHSLVIALIGLTVMMKCRPERNFM 182 H L + + G G + D A +L L G+ + K N M Sbjct: 132 DRVGRHNRKLVARLEGFIRDGQLDGSIRQDVDPAATALALFSYLQGVRIAGKTAELENQM 191 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.132 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,184,223 Number of extensions: 427255 Number of successful extensions: 2317 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 2318 Number of HSP's successfully gapped: 8 Length of query: 196 Length of database: 38,378,431 Length adjustment: 101 Effective length of query: 95 Effective length of database: 26,729,697 Effective search space: 2539321215 Effective search space used: 2539321215 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 113 (48.1 bits)