BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0311.0022.p (392 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value b2681m [GEPR] COG0477 Permeases of the major facilitator superfa... 239 5e-63 CAC0560 [GEPR] COG0477 Permeases of the major facilitator superf... 142 1e-33 SMa1301 [GEPR] COG0477 Permeases of the major facilitator superf... 110 3e-24 STM0328 [GEPR] COG0477 Permeases of the major facilitator superf... 51 4e-06 >b2681m [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 392 Score = 239 bits (610), Expect = 5e-63, Method: Compositional matrix adjust. Identities = 140/369 (37%), Positives = 200/369 (54%), Gaps = 3/369 (0%) Query: 11 LLFAAASGLSVANIYFAQPLLDAIAAEFGIRHSAVGIIITLTQICYAAGXXXXXXXXXXX 70 +L + A+GL+VA+ Y+AQPLLD IA F + S+ G I+T Q+ YAAG Sbjct: 14 VLMSIATGLAVASNYYAQPLLDTIARNFSLSASSAGFIVTAAQLGYAAGLLFLVPLGDMF 73 Query: 71 NRRRLITGQMLLSVLALLIVGTASSGTMXXXXXXXXXXXXXXXXXXXXXXXXXXSPAERG 130 RRRLI LL + LI ++ S M SP +RG Sbjct: 74 ERRRLIVSMTLLXXM--LITASSQSLAMMILGTALTGLFSVVAQILVPLAATLASPDKRG 131 Query: 131 RVVGLVTSGIVIGILLARTFAGLLTDVAGWRAVYLASAGMTLLMAAALYKVLPSVETAKK 190 +VVG + SG+++GILLART AGLL ++ GWR V+ ++ + LMA AL++ LP +++ + Sbjct: 132 KVVGTIMSGLLLGILLARTVAGLLANLGGWRTVFWVASVLMALMALALWRGLPQMKS-ET 190 Query: 191 SLSYLNLIHSVFLLFAHEQILRVRAVLAFLIFTAFSILWTPLVLPLSAPPYSLSHTXXXX 250 L+Y L+ SVF +F ++ILR RA+L L F FSILWT + L+APP++ S Sbjct: 191 HLNYPQLLGSVFSMFISDKILRTRALLGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGL 250 Query: 251 XXXXXXXXXXXXXXXXXXXDRGFGQHTTVIAXXXXXXXXXPISRAEHSLFILAIGIIVLD 310 D+G HTT I S+ L IGI+VLD Sbjct: 251 FGLAGAAGALGARPAGGFADKGKSHHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLD 310 Query: 311 LAVQAVHVTNQSLIFKVRPEARSRLTAGYMIFYSLGSATGSIASTRVYGYAGWNGVCLLG 370 L VQ VH+TNQ++I+++ P+AR+RLTAGYM Y +G A GS+ S + + GW GVCL G Sbjct: 311 LTVQGVHITNQTVIYRIHPDARNRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAG 370 Query: 371 TLVSALALV 379 ++ + L+ Sbjct: 371 ATIALVNLL 379 >CAC0560 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 441 Score = 142 bits (358), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 104/365 (28%), Positives = 171/365 (46%), Gaps = 7/365 (1%) Query: 19 LSVANIYFAQPLLDAIAAEFGIRHSAVGIIITLTQICYAAGXXXXXXXXXXXNRRRLITG 78 ++VAN+Y+ QPLL IA F + + G ++TLTQ+ YA G R++LI Sbjct: 62 VTVANLYYCQPLLGQIAKSFNVTEANAGFVVTLTQVGYALGMLFLVPLGDIKERKKLIIE 121 Query: 79 QMLLSVLALLIVGTASSGTMXXXXXXXXXXXXXXXXXXXXXXXXXXSPAERGRVVGLVTS 138 +LL + L + + + P ERG+V+G V S Sbjct: 122 MLLLVSVFLFAAFISRNYYVLLVTSFLIGFTTIVPQLIVPFAAHLSKPEERGQVIGNVLS 181 Query: 139 GIVIGILLARTFAGLLTDVAGWRAVYLASAGMTLLMAAALYKV---LPSVETAKKSLSYL 195 G++IGILL+R F+G++ D+ GWR VYL + +LLM + L+ + P + K +SY Sbjct: 182 GLLIGILLSRVFSGIIGDILGWRNVYLIA---SLLMVSFLFIIVFLFPKSKPTSK-ISYK 237 Query: 196 NLIHSVFLLFAHEQILRVRAVLAFLIFTAFSILWTPLVLPLSAPPYSLSHTXXXXXXXXX 255 LI S++ L E++LR ++ L+F +FSI WT LV L + Y++ Sbjct: 238 KLIFSMWGLIKREKVLREASINGALMFGSFSIFWTALVFLLESKVYNMGSKEAGFFGLVG 297 Query: 256 XXXXXXXXXXXXXXDRGFGQHTTVIAXXXXXXXXXPISRAEHSLFILAIGIIVLDLAVQA 315 D+ + A + ++ L IG+I+LDL Q Sbjct: 298 IVGALAAPVVGKVADKKTPRFAVGFAIIFSTIAYVCFWGMGYKIYGLVIGVILLDLGNQT 357 Query: 316 VHVTNQSLIFKVRPEARSRLTAGYMIFYSLGSATGSIASTRVYGYAGWNGVCLLGTLVSA 375 V+NQ+ + + + RSR+ +M+ Y +G + GS + + GW+GVC +G Sbjct: 358 GQVSNQARVQAISDQERSRINTVFMVSYFIGGSIGSFVAAVFWQKFGWSGVCAIGMFFQI 417 Query: 376 LALVF 380 +A++F Sbjct: 418 IAVIF 422 >SMa1301 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 385 Score = 110 bits (276), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 107/382 (28%), Positives = 160/382 (41%), Gaps = 5/382 (1%) Query: 14 AAASGLSVANIYFAQPLLDAIAAEFGIRHSAVGIIITLTQICYAAGXXXXXXXXXXXNRR 73 AAA GL AN+Y+ QPL IA + G+ A G+I+TLTQI Y G R Sbjct: 2 AAACGLIAANLYYTQPLAGPIAVDIGLPAEATGLIVTLTQIGYGLGLLLLVPLGDLVENR 61 Query: 74 RLITGQMLLSVLALLIVGTASSGTMXXXXXXXXXXXXXXXXXXXXXXXXXXSPAERGRVV 133 RLI + + LAL+ G +++ A RGRVV Sbjct: 62 RLIVTMIGVVTLALIAAGLSTTPGPFLTASLAIGVGSVAVQMIVPFAANLAPDAARGRVV 121 Query: 134 GLVTSGIVIGILLARTFAGLLTDVAGWRAVYLASAGMTLLMAAALYKVLPSVETAKKSLS 193 G + SG+++GI++AR +GL+ ++ W AV+ SA + + + L+ LP + LS Sbjct: 122 GNIMSGLMVGIMMARPISGLIAGLSSWHAVFYISAIVMVGLGTLLWVQLP-IRMPTARLS 180 Query: 194 YLNLIHSVFLLFAHEQILRVRAVLAFLIFTAFSILWTPLVLPLSAPPYSLSHTXXXXXXX 253 Y L+ S+ L A + +L+ RA F AFS+ WT L L+ P + L H Sbjct: 181 YGQLLKSMAQLLAAQPVLQRRAAYQAFQFAAFSLFWTVTPLYLAGPRFGLGHNGIALFAL 240 Query: 254 XXXXXXXXXXXXXXXXDRGFGQHTTVIAXXXXXXXXXPISRAEHS----LFILAIGIIVL 309 D+G + T A L +L + I+L Sbjct: 241 AGVAGAIASPIAGRLADKGLVRPATAFGLLSVGVAFLVTQIASEGSAIALTLLTLAAILL 300 Query: 310 DLAVQAVHVTNQSLIFKVRPEARSRLTAGYMIFYSLGSATGSIASTRVYGYAGWNGVCLL 369 D V VT Q I+++ E RSRL +M + G A GS + GW ++ Sbjct: 301 DFGVTMTLVTGQRSIYELGAELRSRLNGLFMAIFFTGGAIGSALGAWAFASGGWWFASMI 360 Query: 370 GTLVSALALVFALCSSRIRKDS 391 G + A A+ L ++ S Sbjct: 361 GFALPATAVAIFLTEKHGQERS 382 >STM0328 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 412 Score = 50.8 bits (120), Expect = 4e-06, Method: Compositional matrix adjust. Identities = 75/358 (20%), Positives = 135/358 (37%), Gaps = 12/358 (3%) Query: 20 SVANIYFAQPLLDAIAAEFGIRHSAVGIIITLTQICYAAGXXXXXXXXXXXNRRRLITGQ 79 +A++Y Q LL ++ + I + VG+I++ A R+ +I Sbjct: 28 ELASLYSIQALLPKLSEVYNIPLNQVGMILSAEVGFLALAMLFSGTLSDRFGRKPIIFYS 87 Query: 80 MLLSVLALLIVGTASSGTMXXXXXXXXXXXXX-XXXXXXXXXXXXXSPAERGRVVGLVTS 138 +L + L+ TASS M SPA G V G Sbjct: 88 LLAGGILTLLCATASSWPMLVVYRALLGIAVSGITAAVTVYISEEVSPALAGIVTGYFIF 147 Query: 139 GIVIGILLARTFAGLLTDVAGWRAVYLASAGMTLLMAAALYKVLPSVETAKKSLSYLNLI 198 G +G + R FA L+ + ++ G+ + MA A+ LP T+++ + +L Sbjct: 148 GNSLGSMSGRVFATLMMEHVSIDTIFFIFGGVLIAMALAVKLFLP---TSRQFVPTPSLQ 204 Query: 199 HSVFLLFAHEQILRVRA----VLAFLIFTAFSILWTPLVLPLSAPPYSLSHTXXXXXXXX 254 L E +R V+ F++F +F+ ++ L L PPY LS+T Sbjct: 205 LGAVLKGGLEHFKNIRVSLCFVIGFILFGSFTSIFNFLAFYLHRPPYELSYTWIGLIPVS 264 Query: 255 XXXXXXXXXXXXXXXDRGFGQHTTVIAXXXXXXXXXPISRAEHSLFILAIGIIVLDLAVQ 314 G + ++ SL++ GI++L +A Sbjct: 265 FSLTFFLAPYAARVA-LNIGSMNALSMLIICMMVGAFLTLIAPSLWVFISGIVLLSVAFF 323 Query: 315 AVHVTNQSLIFKVRPEARSRLTAGYMIFYSLGSATGSIASTRVYGYAGWNGV---CLL 369 + H T + + P A+ + T+ Y++ Y G A + ++ + GWN + CL+ Sbjct: 324 SAHSTVLAWVSSRSPNAKGQATSFYLLCYYSGGAVMGYLNGYLFSWQGWNAIAASCLM 381 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.138 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,367,844 Number of extensions: 710708 Number of successful extensions: 2268 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 2260 Number of HSP's successfully gapped: 7 Length of query: 392 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 284 Effective length of database: 25,922,359 Effective search space: 7361949956 Effective search space used: 7361949956 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)