BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0311.0020.p (254 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0818 [M] COG0845 Membrane-fusion protein 138 8e-33 AGc4311 [M] COG1922 Teichoic acid biosynthesis proteins 110 2e-24 slr1271 [M] COG1922 Teichoic acid biosynthesis proteins 109 4e-24 alr1705 [M] COG1922 Teichoic acid biosynthesis proteins 105 7e-23 all4422 [M] COG1922 Teichoic acid biosynthesis proteins 97 2e-20 >STM0818 [M] COG0845 Membrane-fusion protein Length = 331 Score = 138 bits (348), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 11/220 (5%), Positives = 38/220 (17%), Gaps = 3/220 (1%) Query: 12 VSILGVRFSKLTLEETVLHLAEQIENKRDKLFHLITANPEIXXXXXXLLQRIIQSADMVV 71 + + LA ++ D + L + + Sbjct: 35 LYGNVDIRTVNISFRVGGRLASLNVDEGDTIKAGQVLGELDHAPYENALMQAKAGVSVAQ 94 Query: 72 PDGIGIVLAARRQGNPIPERVTGYDLLLKLLEQGNERTWSFYFLGTDEETSRQAVEKITH 131 ++ R + Q + + + + Sbjct: 95 AQYDLMLAGYRDEEIAQAAAAVRQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSR 154 Query: 132 SYPKVRIAGRHHGFFDAAEEPEIIAGIQQSRPDILILAMGAPYSDKWLHKHKDELAGVKL 191 + + + + L A + + + Sbjct: 155 D--QAQATLKSAQDKLSQYRTGNREQDIAQAKASLEQAKAQLAQAQLDLQDTTLI-APAN 211 Query: 192 VFGVGGSLDVISGKVKPTPPVWKKLNLEWAHRLLFSPVAK 231 + +++ S + + L R Sbjct: 212 GTLLTRAVEPGSMLNAGSTVLTLSLTRPVWVRAYVDERNL 251 >AGc4311 [M] COG1922 Teichoic acid biosynthesis proteins Length = 211 Score = 110 bits (275), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 0/173 (0%), Positives = 0/173 (0%), Gaps = 1/173 (0%) Query: 63 IIQSADMVVPDGIGIVLAARRQG-NPIPERVTGYDLLLKLLEQGNERTWSFYFLGTDEET 121 + + + + + + + RT ++ Sbjct: 101 NGEVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE 160 Query: 122 SRQAVEKITHSYPKVRIAGRHHGFFDAAEEPEIIAGIQQSRPDILILAMGAPYSDKWLHK 181 Sbjct: 161 220 Query: 182 HKDELAGVKLVFGVGGSLDVISGKVKPTPPVWKKLNLEWAHRLLFSPVAKGQK 234 Sbjct: 221 273 >slr1271 [M] COG1922 Teichoic acid biosynthesis proteins Length = 246 Score = 109 bits (273), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 75/254 (29%), Positives = 125/254 (49%), Gaps = 19/254 (7%) Query: 6 EPMNGTVSILGVRFSKLTLEETVLHLAEQIENKRDKLFHLITANPEIXXXXXX--LLQRI 63 EP T S+ GV K+ L + H E +R + H++T N E+ +Q + Sbjct: 7 EPSLPTASVFGV---KVHLSDNYGHWLENC-LQRGQGVHVVTLNSEMVMLAEKEPAVQEV 62 Query: 64 IQSADMVVPDGIGIVLAARRQGNPIPERVTGYDL---LLKLLEQGNERTWSFYFLGTDEE 120 IQ A++V+PDG G+ + + +G R G +L +++ + +G + F G Sbjct: 63 IQGAELVIPDGAGVTIYLKLEGIE-QSRCPGIELAEAMVRHIGEGKDNQ-PIAFYGGAPG 120 Query: 121 TSRQAVEKITHSYPKVRIAGRHHGFFDAAEEPEIIAGIQQSRPDILILAMGAPYSDKWLH 180 + A ++ YP ++I +HGF +E+ + + RP ++++ +G P + W+ Sbjct: 121 ITELAAQRWQREYPGLKILA-NHGFLTESEQDAWQQTLARERPCLILVGLGVPRQEYWIR 179 Query: 181 KHKDELAGVKLVFGVGGSLDVISGKVKPTPPVWKKLNLEWAHRLLFSPVAKGQKSRWRRQ 240 H+ L + GVGGS DV SG + P + + NLEW +RL P RWRR Sbjct: 180 DHR-HLCPQGIWIGVGGSFDVWSGTKQRAPEFFCRYNLEWFYRLYQEP------WRWRRM 232 Query: 241 SALPKFVYRTMIRR 254 ALPKF +RT++ R Sbjct: 233 LALPKFFFRTLVSR 246 >alr1705 [M] COG1922 Teichoic acid biosynthesis proteins Length = 359 Score = 105 bits (262), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 15/224 (6%), Positives = 36/224 (16%), Gaps = 17/224 (7%) Query: 38 KRDKLFHLITANPEIXXXXX--XLLQRIIQSADMVVPDGIGIVLAARRQGNPIPERVTGY 95 + + + + + GI R Sbjct: 30 VAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPT 89 Query: 96 DLLLKLL-----EQGNERTWSFYFLGTDEETSRQAVEKITHSYPKVRIAGRHHGFFDAAE 150 E+ + DE + VR + + Sbjct: 90 ISAQHFHTAVHNEEQLA---ALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQ 146 Query: 151 EPEIIAGIQQSRPDILILAMGAPYSDKWLHKHKDELAGVKLVFGVGGSLDVISGKVKPTP 210 + + ++K L K + I + Sbjct: 147 CKNVRQPVNIVSHFARADEPKCGATEKQLAIFN-TFCEGKPGQRSIAASGGILLWPQSHF 205 Query: 211 PVWKKLNLEWAHRLLFSPVAKGQKSRWRRQSALPKFVYRTMIRR 254 + + + L S P + + Sbjct: 206 DWVRPGIILYGVSPLEDR------STGADFGCQPVMSLTSSLIA 243 >all4422 [M] COG1922 Teichoic acid biosynthesis proteins Length = 257 Score = 97.1 bits (240), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 65/250 (26%), Positives = 115/250 (46%), Gaps = 15/250 (6%) Query: 11 TVSILGVRFSKLTLEETVLHLAEQIENKRDKLFHLITANPEIXXXXXXLLQRIIQSADMV 70 + +LGV L++ E + E IE + + + +Q A+ + Sbjct: 6 SYKLLGVSVDALSIPELNCLIGESIEQGKKWIIANHNLHSLYLFHKDLEMQAFYAKAEYI 65 Query: 71 VPDGIGIVLAARRQGNPIP--ERVTGYDLLLKLLEQGNERTWSFYFLGTDEETSRQAVEK 128 D + IV + G P+ +RVT D + L+E+ ++W ++LG+ + Q Sbjct: 66 HIDSMPIVFIGKLLGFPMKREQRVTYADWVWPLMEEAANKSWRIFYLGSKPGVAEQGAVI 125 Query: 129 ITHSYPKVRIAGRHHGFFDAAEEPE----IIAGIQQSRPDILILAMGAPYSDKWLHKHKD 184 + +P ++IA HG+FD +EE E +I I +P +L++ MG P + W+ ++ + Sbjct: 126 LRRKFPGLKIACS-HGYFDTSEESEENLTVIKAINDYKPHVLMVGMGMPRQEHWISQNLE 184 Query: 185 ELAGVKLVFGVGGSLDVISGKVKPTPPVWK-KLNLEWAHRLLFSPVAKGQKSRWRRQSAL 243 + + G LD ++G + PTPP W ++ LEW +RLL P WRR Sbjct: 185 RIQP-NAILTSGACLDYVAGAI-PTPPRWMGRMGLEWLYRLLTEPT-----RLWRRYLLE 237 Query: 244 PKFVYRTMIR 253 P F+ +R Sbjct: 238 PWFLGTLFLR 247 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,690,821 Number of extensions: 874665 Number of successful extensions: 1369 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 1357 Number of HSP's successfully gapped: 6 Length of query: 254 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 150 Effective length of database: 26,383,695 Effective search space: 3957554250 Effective search space used: 3957554250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)