BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0311.0014.p (217 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPy0141 [I] COG1788 Acyl CoA:acetate/3-ketoacid CoA transferase,... 264 1e-70 SPy1638 [I] COG1788 Acyl CoA:acetate/3-ketoacid CoA transferase,... 261 5e-70 FN1857 [I] COG1788 Acyl CoA:acetate/3-ketoacid CoA transferase, ... 239 3e-63 BS_yxjD [I] COG1788 Acyl CoA:acetate/3-ketoacid CoA transferase,... 181 7e-46 RSc1570 [I] COG1788 Acyl CoA:acetate/3-ketoacid CoA transferase,... 174 8e-44 >SPy0141 [I] COG1788 Acyl CoA:acetate/3-ketoacid CoA transferase, alpha subunit Length = 219 Score = 264 bits (674), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 128/215 (59%), Positives = 155/215 (72%) Query: 2 NKVVALDEAIAHIKDGDVVMIGGFMANGTPEKLVDALVEANIKDITLICNDTGFINRGSG 61 NK +A+ EAI+HIKDGD +M+GGFMANGTPE L+DALV+ KD+TLICND GF++RG G Sbjct: 4 NKRIAIAEAISHIKDGDTIMVGGFMANGTPEALIDALVDKGTKDLTLICNDAGFVDRGVG 63 Query: 62 KLVSNKQCKKIMASHIGTNKETGRQMTEGETEVVLIPQGTLVEQIRAGGYGLGGVLTATG 121 K+V+N Q K I A+HIG NKE GRQMT GET + LIPQGT E+IR G YG+GG T TG Sbjct: 64 KMVANHQFKTIYATHIGLNKEAGRQMTAGETTIELIPQGTFAEKIRIGAYGIGGFYTPTG 123 Query: 122 IGTLVEEGKEKIVVDGKEYLLEKPITADVALLYAAKADKAGNMVYKGSANNFNNIMAGAA 181 +GTLV EGKE + GK YLLE P ADVAL++A +AD+ GN+ Y GS NNFN +MA A Sbjct: 124 VGTLVAEGKETKTIKGKTYLLEYPFEADVALIFANQADEMGNLQYSGSENNFNQLMAACA 183 Query: 182 KTTXXXXXXXXXXXXXDPNEVVTPNIFVNYIVEGG 216 KTT P V TP+IFV+YIV+ G Sbjct: 184 KTTIVQAREIVPVGTIQPECVHTPHIFVDYIVKEG 218 >SPy1638 [I] COG1788 Acyl CoA:acetate/3-ketoacid CoA transferase, alpha subunit Length = 216 Score = 261 bits (667), Expect = 5e-70, Method: Compositional matrix adjust. Identities = 135/215 (62%), Positives = 166/215 (77%) Query: 1 MNKVVALDEAIAHIKDGDVVMIGGFMANGTPEKLVDALVEANIKDITLICNDTGFINRGS 60 M K + + EA+AH+KDGD +M+GGFM NGTPEKL+DALVE +KD+TLICND GF ++G Sbjct: 1 MCKEITIKEAVAHVKDGDTIMVGGFMTNGTPEKLIDALVEKGVKDLTLICNDAGFPDKGV 60 Query: 61 GKLVSNKQCKKIMASHIGTNKETGRQMTEGETEVVLIPQGTLVEQIRAGGYGLGGVLTAT 120 GK+V+NKQ I+ASHIG N+E GRQMTEGET + L+PQGTL E+IR+GG+GLGG LT T Sbjct: 61 GKMVANKQFSTIIASHIGLNREAGRQMTEGETVIDLVPQGTLAERIRSGGFGLGGFLTPT 120 Query: 121 GIGTLVEEGKEKIVVDGKEYLLEKPITADVALLYAAKADKAGNMVYKGSANNFNNIMAGA 180 GIGT V +GKE I +DGK+YLLEKP+ ADVAL++A KADK GN+ Y GS NNFN++MA Sbjct: 121 GIGTEVAKGKEVITIDGKDYLLEKPLKADVALIFANKADKNGNLQYAGSENNFNHVMAAN 180 Query: 181 AKTTXXXXXXXXXXXXXDPNEVVTPNIFVNYIVEG 215 AKTT DPN V TP IFVNY+V+G Sbjct: 181 AKTTIVEAREIVDVGQMDPNFVHTPGIFVNYLVKG 215 >FN1857 [I] COG1788 Acyl CoA:acetate/3-ketoacid CoA transferase, alpha subunit Length = 217 Score = 239 bits (609), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 113/213 (53%), Positives = 154/213 (72%) Query: 2 NKVVALDEAIAHIKDGDVVMIGGFMANGTPEKLVDALVEANIKDITLICNDTGFINRGSG 61 K+V+++EAI+H+KDG V IGGF+A GTPE ++ AL+E +KD+T++ NDTG I++G G Sbjct: 3 QKIVSMEEAISHVKDGMTVHIGGFIACGTPESIITALIEKGVKDLTIVANDTGLIDKGIG 62 Query: 62 KLVSNKQCKKIMASHIGTNKETGRQMTEGETEVVLIPQGTLVEQIRAGGYGLGGVLTATG 121 +LV N Q KK++ASHIGTN ETGR+M GE EV L+PQGTL E++RA GYGLGG+LT TG Sbjct: 63 RLVVNNQVKKVIASHIGTNPETGRRMQSGEMEVELVPQGTLAERVRAAGYGLGGILTPTG 122 Query: 122 IGTLVEEGKEKIVVDGKEYLLEKPITADVALLYAAKADKAGNMVYKGSANNFNNIMAGAA 181 +GT+V+EGK+ I VDGK+YLLEKPI ADVAL++ K D+ GN++ + + NFN +MA AA Sbjct: 123 LGTIVQEGKQIINVDGKDYLLEKPIKADVALIFGTKVDELGNVICEKTTKNFNPLMATAA 182 Query: 182 KTTXXXXXXXXXXXXXDPNEVVTPNIFVNYIVE 214 P + IF++YIV+ Sbjct: 183 DVVIVEALEIVPAGSLSPEHLDISRIFIDYIVK 215 >BS_yxjD [I] COG1788 Acyl CoA:acetate/3-ketoacid CoA transferase, alpha subunit Length = 238 Score = 181 bits (459), Expect = 7e-46, Method: Compositional matrix adjust. Identities = 96/216 (44%), Positives = 137/216 (63%), Gaps = 1/216 (0%) Query: 1 MNKVVALD-EAIAHIKDGDVVMIGGFMANGTPEKLVDALVEANIKDITLICNDTGFINRG 59 M KV++ EA I DGD ++ GGF G PE+L+ ++ + +KD+T++ N+ G + G Sbjct: 1 MGKVLSSSKEAAKLIHDGDTLIAGGFGLCGIPEQLILSIRDQGVKDLTVVSNNCGVDDWG 60 Query: 60 SGKLVSNKQCKKIMASHIGTNKETGRQMTEGETEVVLIPQGTLVEQIRAGGYGLGGVLTA 119 G L++NKQ KK++AS++G NK RQ GE EV L+PQGTL E+IRAGG G+ G TA Sbjct: 61 LGLLLANKQIKKMIASYVGENKIFERQFLSGELEVELVPQGTLAERIRAGGAGIPGFYTA 120 Query: 120 TGIGTLVEEGKEKIVVDGKEYLLEKPITADVALLYAAKADKAGNMVYKGSANNFNNIMAG 179 TG+GT + EGKE G+ Y+LE+ IT DVA++ A KAD GN++++ +A NFN I A Sbjct: 121 TGVGTSIAEGKEHKTFGGRTYVLERGITGDVAIVKAWKADTMGNLIFRKTARNFNPIAAM 180 Query: 180 AAKTTXXXXXXXXXXXXXDPNEVVTPNIFVNYIVEG 215 A K T DP+ + TP I+V ++V G Sbjct: 181 AGKITIAEAEEIVEAGELDPDHIHTPGIYVQHVVLG 216 >RSc1570 [I] COG1788 Acyl CoA:acetate/3-ketoacid CoA transferase, alpha subunit Length = 232 Score = 174 bits (441), Expect = 8e-44, Method: Compositional matrix adjust. Identities = 95/215 (44%), Positives = 133/215 (61%), Gaps = 2/215 (0%) Query: 1 MNKVVA-LDEAIAHI-KDGDVVMIGGFMANGTPEKLVDALVEANIKDITLICNDTGFINR 58 MNK+ A EA+A I KDG + +GGF G PE L+ AL ++ K +T I N+ G Sbjct: 1 MNKIYASAAEALAGIVKDGQTIAVGGFGLCGIPEALIGALRDSGAKGLTCISNNAGVDGF 60 Query: 59 GSGKLVSNKQCKKIMASHIGTNKETGRQMTEGETEVVLIPQGTLVEQIRAGGYGLGGVLT 118 G G+L+ +Q KK+++S++G NKE RQ GE E+ PQGTL E++RAGG G+ T Sbjct: 61 GLGRLLETRQIKKMISSYVGENKEFERQYLSGELELEFTPQGTLAEKLRAGGAGIPAFFT 120 Query: 119 ATGIGTLVEEGKEKIVVDGKEYLLEKPITADVALLYAAKADKAGNMVYKGSANNFNNIMA 178 TG+GT+V +GKE DG++Y++E+ + ADVAL+ A KADK+GN+ Y +A NFN + A Sbjct: 121 RTGVGTVVADGKEVREFDGEQYVMERSLIADVALVKAWKADKSGNLKYHRTARNFNPMCA 180 Query: 179 GAAKTTXXXXXXXXXXXXXDPNEVVTPNIFVNYIV 213 A + T DP+EV TP +FV IV Sbjct: 181 MAGRITIVEVEEIVETGTFDPDEVHTPGVFVQRIV 215 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.134 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,460,059 Number of extensions: 643070 Number of successful extensions: 1268 Number of sequences better than 1.0e-05: 22 Number of HSP's gapped: 1260 Number of HSP's successfully gapped: 22 Length of query: 217 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 115 Effective length of database: 26,614,363 Effective search space: 3060651745 Effective search space used: 3060651745 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 113 (48.1 bits)