BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0311.0001.p (500 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CC2473 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A su... 287 3e-77 SPAC869.01 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase ... 263 5e-70 lin0842 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 252 8e-67 DR1625 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A su... 250 5e-66 RSc0057 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 154 5e-37 >CC2473 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 497 Score = 287 bits (734), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 176/460 (38%), Positives = 249/460 (54%), Gaps = 26/460 (5%) Query: 60 VNSVLEINPDALFIAEALDVERSLQGPRGPLHGIPVLLKDNINTGDKMHTSAGSLALADS 119 + +V+ NP+AL A+ALD ER R LHG+P+LLKDNI + D + T+AGSLAL D+ Sbjct: 43 LRAVIATNPNALTAAKALDAERKAGKVRSALHGVPILLKDNIESADPLPTTAGSLALKDN 102 Query: 120 FAGEDAFIVTRLRDAGAVIMGKANMTEFANFMTNGMPSGYSSRGGQVLNPYNISTPTXXX 179 G DA + RLRDAG VI+GKAN++E+AN +N SG+S+ GG V NPY + Sbjct: 103 ITGRDAPVAKRLRDAGLVILGKANLSEWANIRSNHSISGWSAVGGTVRNPYALDRSACGS 162 Query: 180 XXXXXXXXXCNFCTVSVGTETSGSILNPGNLGSIIGIKPTVGMLSRSGILPLSNTQDTAG 239 +++GTET GSI P + ++G+KPTVG++SR+ I+P+S++QDTAG Sbjct: 163 SSGSGAAVAAGLAPLAIGTETDGSITCPAAINGLVGLKPTVGLVSRTHIVPISHSQDTAG 222 Query: 240 PMARTVRDAVLLLNAMLGKDDSDAAMGTGVGRLHEDYTVFLDPNGLQGARIGIPRDYY-F 298 PM TV D +L + G D +D A R DY L L+G + + R Y + Sbjct: 223 PMTLTVEDTAKVLTIIAGSDPTDPATKEADAR-KTDYAAGLSKTALKGVTLAVARFYTGY 281 Query: 299 EELTDEQLALFNASVERMRGLGATIIDPADIRTAREIKYSSVVL-NEFKTSLNAYLARLG 357 TD A+F +++ + GAT++D + VVL E K L AYLA Sbjct: 282 SPKTD---AVFERALKELEAQGATLVDVKAFDEGPIGRAEGVVLYTELKADLAAYLASTD 338 Query: 358 P-GAPMRTLKDIIDFNHAHPMETLRYGQSTLLDAEYTSSGTFTEPQYLRDRATDLRLCKE 416 P P RTL D+I FN A P E +GQ + AE T +P+YL+ A RL Sbjct: 339 PKKVPTRTLADVIAFNKATPKEFEWFGQESFERAEKTKG--LDDPEYLKALADSKRLAGP 396 Query: 417 LGIDAAMKEHQLDALLFPA-------------DFGARITSR---AGYPSIVVPSGYTSAG 460 GID +K+ A++ P ++G T+ AGYP + VP G S G Sbjct: 397 EGIDKILKDTGAVAIIAPTTGPAWTIDPLNGDNYGGSSTTLPAVAGYPHLTVPMGDVS-G 455 Query: 461 VPFGVTFSARAYQEPVLIRLAYAYEQNYKIRKAPSLRSFL 500 +P G++F A+ E +L+ L YAYEQ K RKAP+ ++ + Sbjct: 456 LPVGLSFIGPAWSEKLLLNLGYAYEQATKHRKAPTFKATI 495 >SPAC869.01 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 583 Score = 263 bits (672), Expect = 5e-70, Method: Compositional matrix adjust. Identities = 170/518 (32%), Positives = 267/518 (51%), Gaps = 48/518 (9%) Query: 22 EATIPEIQAALETGQMTSRQLVLMYLERIAEHDKSGLTVNSVLEINPDALFIAEALDVER 81 +ATI ++Q +E G +TS +V YL+R + + VN +L++NPD L IA LD ER Sbjct: 68 DATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPY---VNGILQLNPDVLTIASELDDER 124 Query: 82 SLQGPRGPLHGIPVLLKDNINTGDKMHTSAGSLALADSFAGEDAFIVTRLRDAGAVIMGK 141 + RGPLHGIP ++KDN T DKM T+AGS AL S DA++V +LR+AGAV+ G Sbjct: 125 ANGIIRGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAVLFGH 184 Query: 142 ANMTEFANFMTNGMPSGYSSRGGQVLNPYNISTPTXXXXXXXXXXXXCNFCTVSVGTETS 201 A ++E+A+ +N GYS+RGGQ P+N++ N ++GTET Sbjct: 185 ATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFALGTETD 244 Query: 202 GSILNPGNLGSIIGIKPTVGMLSRSGILPLSNTQDTAGPMARTVRDAVLLLNAMLGKDDS 261 GSI++P ++G+KPTVG+ SR G++P S QDT GP+ARTVRDAV + +M G D++ Sbjct: 245 GSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSMWGIDEN 304 Query: 262 DAAMGTGVGRLHE--DYTVFL-DPNGLQGARIGIPRDYYFEELTDEQLALFNASVERMRG 318 D G+ E DY FL + L+GAR G+P ++ +++ V+++ Sbjct: 305 DIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKADEIDRLLEVVKQIEE 364 Query: 319 LGATIIDPA---------------DIRTAREIKYSSVVLNEFKTSLNAYLARLGPGAPMR 363 GA + + ++ + E +Y +VV +F ++ +YL+ + + Sbjct: 365 AGAIVYNNTNFYNLDVISNDGWNWELGSVNESEY-TVVKVDFYNNIKSYLSEV-KNTEIH 422 Query: 364 TLKDIIDFNHAHP---------METLRYGQSTLLDAEYTSSGTFTEPQYLRDRATDLRLC 414 +L+DI+++N+ + + GQ L + G Y + R Sbjct: 423 SLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLAS--LEWGGVKNETYWQAVEYVRRTS 480 Query: 415 KELGIDAAMKEHQ--------LDALLFPADFGARIT----SRAGYPSIVVPSGYTSAGVP 462 ++ GID A+ L+ LL P+ G IT ++AGYP I +P G + G P Sbjct: 481 QDEGIDYALNYTDPKTNDSFILNGLLVPS--GTSITYQQAAKAGYPMITLPIGVKTNGRP 538 Query: 463 FGVTFSARAYQEPVLIRLAYAYEQNYKIRKAPSLRSFL 500 FG+ A+QEP LI+ A E + + P ++ Sbjct: 539 FGLGIMHSAWQEPQLIKYGSAIEDLLQYKAKPKFYEYV 576 >lin0842 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 301 Score = 252 bits (644), Expect = 8e-67, Method: Compositional matrix adjust. Identities = 0/482 (0%), Positives = 0/482 (0%), Gaps = 34/482 (7%) Query: 20 IVEATIPEIQAALETGQMTSRQLVLMYLERIAEHDKSGLTVNSVLEINPDALFIAEALDV 79 + A+ + R L + + ++ + + Sbjct: 184 YITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILVEREGQK 243 Query: 80 ERSLQGPRGPLHGIPVLLKDNINTGDKMHTSAGSLALADSFAGEDAFIVTRLRDAGAVIM 139 + + + ++ + +M + L L A LR G V Sbjct: 244 KMVIGNK--GAKIKTIGIEARKDMQ-EMFEAPVHLELWVKVKSGWADDERALRSLGYVDD 300 Query: 140 GKANMTEFANFMTNGMPSGYSSRGGQVLNPYNISTPTXXXXXXXXXXXXCNFCTVSVGTE 199 Sbjct: 301 360 Query: 200 TSGSILNPGNLGSIIGIKPTVGMLSRSGILPLSNTQDTAGPMARTVRDAVLLLNAMLGKD 259 Sbjct: 361 --- 417 Query: 260 DSDAAMGTGVGRLHEDYTVFLDPNGLQGARIGIPRDYYFEELTDEQLALFNASVERMRGL 319 Sbjct: 418 ----------------- 460 Query: 320 GATIIDPADIRTAREI--KYSSVVLNEFKTSLNAYLARLGPGAPMRTLKDIIDFNHAHPM 377 Sbjct: 461 -- 518 Query: 378 ETLRYGQSTLLDAEYTSSGTFTEPQYLRDRATDLRLCKELGIDAAMKEHQLDALLFPADF 437 Sbjct: 519 ---- 574 Query: 438 GAR--ITSRAGYPSIVVPSGY-TSAGVPFGVTFSARAYQEPVLIRLAYAYEQNYKIRKAP 494 Sbjct: 575 634 Query: 495 SL 496 Sbjct: 635 636 >DR1625 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 546 Score = 250 bits (638), Expect = 5e-66, Method: Compositional matrix adjust. Identities = 174/457 (38%), Positives = 248/457 (54%), Gaps = 30/457 (6%) Query: 20 IVEATIPEIQAALETGQMTSRQLVLMYLERIAEHDKSGLTVNSVLEINPDALFIAEALDV 79 I++ + + A G +T ++ YL R+ + + +V+ +NP A A+ALD Sbjct: 74 ILDLDVCALAGATRRGDLTPSEVTRTYLARLTAINPE---LRAVITVNPGAQATADALDD 130 Query: 80 ERSLQGPRGPLHGIPVLLKDNINTGDKMHTSAGSLALADSFAGEDAFIVTRLRDAGAVIM 139 Q RG LHG+P+L+KDNI+ + T+AGS+ L DA +V RLR AGAVI+ Sbjct: 131 IPEKQ--RGVLHGVPLLIKDNIDVA-GLPTTAGSVLLRGHVPERDAPLVARLRAAGAVIL 187 Query: 140 GKANMTEFANFMTNGMPSGYSSRGGQVLNPYNISTPTXXXXXXXXXXXXCNFCTVSVGTE 199 GKANMTE+ANFMT GMP+GYSS GGQ +NP+ T C ++GTE Sbjct: 188 GKANMTEWANFMTLGMPNGYSSLGGQTVNPWGPERDTGGSSSGSGVAVAARLCAAAIGTE 247 Query: 200 TSGSILNPGNLGSIIGIKPTVGMLSRSGILPLSNTQDTAGPMARTVRDAVLLLNAMLGKD 259 TSGS+++P + +IG+KPT+G++ R+GI+P+S++QDTAGP+ R+VRDA LL+ M G D Sbjct: 248 TSGSVVSPAHQNGVIGLKPTLGLIPRTGIIPISHSQDTAGPLTRSVRDAALLMTVMSGPD 307 Query: 260 DSDAAMGTGVGRLHEDYTVFLDPNGLQGARIGIPRDYYFEELTDEQLALFNASVERMRGL 319 D+DAA D TV L G +I D ++ + A+ + + Sbjct: 308 DADAASQL----FTPDLTV--SEKALSGTKIAAIHDE--PNASEAERAVLKRAEAALAAA 359 Query: 320 GATIIDPADIRTAREIKYSS----VVLNEFKTSLNAYLARLGPGAPMRTLKDIIDFNHAH 375 GAT+ A T E+K + V++ EFK LNAYLA + G +L +I+ N Sbjct: 360 GATVNGTA-FPTRAELKAAGWRLDVLVYEFKPDLNAYLAGVKQGP--TSLAAVIEGNDED 416 Query: 376 PMETLRYGQSTLLDAEYTSSGTFTEPQYLRDRATDLRLCKELGIDAAMKEHQLDALLFPA 435 P LRYGQ +L A+ T G +E Y R R DLRL + G DA + A+LFP Sbjct: 417 PAARLRYGQHLMLAAQAT-RGDLSEGAYARARERDLRLARTQGFDALFAQGH-GAVLFPG 474 Query: 436 DFGARITSRAGYPSIVVP-------SGYTSAGVPFGV 465 G + + AGYPS+ +P +G G P GV Sbjct: 475 IHGCDLAAAAGYPSLALPVPLGDGANGEAPHGQPSGV 511 >RSc0057 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 495 Score = 154 bits (388), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 137/504 (27%), Positives = 231/504 (45%), Gaps = 43/504 (8%) Query: 20 IVEATIPEIQAALETGQMTSRQLVLMYLERIAEHDKSGLTVNSVLEINPDALFIAEALDV 79 + +T+ + A L ++++ +L YL RI ++ +N+ + ++P+A Sbjct: 1 MTASTLKTLSAQLAAKEVSAVELARHYLARI----EARADLNAFIHVDPEATLAQAQAAD 56 Query: 80 ERSLQGPRGPLHGIPVLLKDNINTGDKMHTSAGSLALADSFAGEDAFIVTRLRDAGAVIM 139 R G GPL GIP+ KD T +AGS L + DA +V RL AG V + Sbjct: 57 ARLAAGDAGPLAGIPIAHKDVFVT-RGWRATAGSKMLDSYVSPFDATVVERLAAAGMVTL 115 Query: 140 GKANMTEFANFMTNGMPSGYSSRGGQVLNPYNISTPTXXXXXXXXXXXXCNFCTVSVGTE 199 GK NM EFA +N +S G V NP++++ + + GT+ Sbjct: 116 GKTNMDEFAMGSSN-----ENSHFGPVKNPWDVARVPGGSSGGSAAAVAADLAPAATGTD 170 Query: 200 TSGSILNPGNLGSIIGIKPTVGMLSRSGILPLSNTQDTAGPMARTVRDAVLLLNAMLGKD 259 T GSI P + I GIKPT G +SR G++ +++ D GPMART D LLL+AM G D Sbjct: 171 TGGSIRQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMARTAEDCALLLSAMAGFD 230 Query: 260 DSDA-AMGTGVGRLHEDYTVFLD--------PNGLQGARIGIPRDYYFEELTDEQLALFN 310 D+ ++ G G ED+ L L G RIG+P++Y+ L D+ Sbjct: 231 ARDSTSLEPGRGGDAEDFGRLLGRPLEGADAARPLAGLRIGLPQEYFGAGLADDVRTAVR 290 Query: 311 ASVERMRGLGATIIDPADIRTAREI-KYSSVVLNEFKTSLNAYLA-RLGPGAPMRTLKDI 368 A++ + LGAT++D + +T I Y + E ++L+ + R G A +D+ Sbjct: 291 AALAELETLGATLVDISLPKTELSIPTYYVIAPAEASSNLSRFDGVRYGHRAA--AYRDL 348 Query: 369 IDFNHAHPMETLRYG-QSTLLDAEYTSSGTFTEPQYLRDRATDLRLCKELG--------- 418 D E + + +L Y S + + YL+ + + ++ Sbjct: 349 ADMYRKTRAEGFGWEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQNAFGQCDVI 408 Query: 419 -------IDAAMKEHQLDAL-LFPADFGARITSRAGYPSIVVPSGYTSAGVPFGVTFSAR 470 + + E D L ++ AD TS AG P + VP+G+ + G+P G+ Sbjct: 409 MGPVAPTVAWKLGEKTDDPLQMYLADIFTLSTSLAGLPGMSVPAGFGANGLPVGLQIIGN 468 Query: 471 AYQEPVLIRLAYAYEQ--NYKIRK 492 ++E ++++A+A++Q ++ R+ Sbjct: 469 YFEEARMLQIAHAFQQATDWHTRR 492 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 35,758,305 Number of extensions: 1398049 Number of successful extensions: 2743 Number of sequences better than 1.0e-05: 68 Number of HSP's gapped: 2593 Number of HSP's successfully gapped: 69 Length of query: 500 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 389 Effective length of database: 25,576,357 Effective search space: 9949202873 Effective search space used: 9949202873 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)