BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0073.p (396 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydfH [T] COG4585 Signal transduction histidine kinase 274 2e-73 CAC3430 [T] COG4585 Signal transduction histidine kinase 273 3e-73 all4636 [T] COG4585 Signal transduction histidine kinase 113 7e-25 CAC2940 [T] COG4585 Signal transduction histidine kinase 96 1e-19 alr2137_3 [T] COG4585 Signal transduction histidine kinase 89 1e-17 >BS_ydfH [T] COG4585 Signal transduction histidine kinase Length = 228 Score = 274 bits (700), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 0/382 (0%), Positives = 0/382 (0%), Gaps = 5/382 (1%) Query: 12 DLFASRLPTLIWVSIVYVGTIVLQFLREHL-ILESAVFTGLFTIHVLLHWNSYRVTYKKF 70 L + LI V + A + + K Sbjct: 20 ILRRDIVHCLIAPGTPLSEKEVSVRFNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKIS 79 Query: 71 WIYFSVQAALIYLCAILMRDGYQAVLIGLLPILIAQTLSYSFRVKRXXXXXXXXXXXXXX 130 + + + +++ + Q L Sbjct: 80 MAQVRNGSFIRQAIECAVARRAASMITESQCYQLEQNLHQQRI-AIERKQLDDFFELDDN 138 Query: 131 XALTVGDRDELIVFLPIFLLMLIVVLAYAILFFKQVQERLRIQSFLEDLREAHEKVEELT 190 + + + L + + + + + Sbjct: 139 FHQLL-TQIADCQLAWDTIENLKATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGD 197 Query: 191 LANERQRMARDLHDTLAQGVAGLIMRLEAADAHMSQNHPERAQEIIRQSMQQARRTLAEA 250 Sbjct: 198 257 Query: 251 RRAIDNLRLKSAPEMDFKEAIADEIEHFREATGIVVSTDYRLNKRLSRLQMEHSLHIVKE 310 Sbjct: 258 317 Query: 311 CLTNIARHAKADKVWVFV-STQNDKLIIEIQDNGIGFKTDDIGKDAGHYGLLGIKERVRL 369 Sbjct: 318 377 Query: 370 IGGEIYVNSNS-EGTRVKLETP 390 Sbjct: 378 399 >CAC3430 [T] COG4585 Signal transduction histidine kinase Length = 397 Score = 273 bits (699), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 145/375 (38%), Positives = 247/375 (65%), Gaps = 2/375 (0%) Query: 17 RLPTLIWVSIVYVGTIVLQFLREHLILESAVFTGLFTIHVLLHWNSYRVTYKKFWIYFSV 76 RL ++I + +V V ++ LQ ++E + +S +FT + + +++W SY + ++WIYF + Sbjct: 14 RLLSIICIILVCVFSLFLQNIKEIYLFQSIIFTLIMILFFIMYWFSY-IFKNRYWIYFLI 72 Query: 77 QAALIYLCAILMRDGYQAVLIGLLPILIAQTLSYSFRVKRXXXXXXXXXXXXXXXALTVG 136 Q +I CA L++ GY A+ IG++PIL++Q++ + + + + + Sbjct: 73 QGIIICACAFLIQKGYHALFIGIIPILMSQSVELYYNIFKIILTFIFLYSIFCGTVVIIN 132 Query: 137 DRDELIVFLPIFLLMLIVVLAYAILFFKQVQERLRIQSFLEDLREAHEKVEELTLANERQ 196 L +P+FL+++I V+ Y+ +FFKQV+ R++ Q L++L A++KVEELTL NERQ Sbjct: 133 GAYYLPELIPVFLIIIIFVIGYSAIFFKQVKLRIQTQKVLKELELAYDKVEELTLTNERQ 192 Query: 197 RMARDLHDTLAQGVAGLIMRLEAADAHMSQNHPERAQEIIRQSMQQARRTLAEARRAIDN 256 RMARDLHDTL+QG+AG+IM+LEA +A++++N+ +RAQEI+ ++M+ AR+TLA++R I++ Sbjct: 193 RMARDLHDTLSQGIAGIIMQLEAVNANLNKNNNKRAQEIVLKAMEHARKTLADSRLVIED 252 Query: 257 LRLKSAPEMDFKEAIADEIEHFREATGIVVSTDYRLNKRLSRLQMEHSLHIVKECLTNIA 316 LR ++ DF + + E+ FR + V D ++ +++ +EH L+I++ECL NIA Sbjct: 253 LRSETDLNGDFIDLVKSEVSKFRNLSSSEVEVDIQMKSQITTKILEHLLYIIRECLNNIA 312 Query: 317 RHAKADKVWVFVSTQNDKLIIEIQDNGIGFKTDDIGKDAGHYGLLGIKERVRLIGGEIYV 376 +HAKA+ V + + ++ ++ +E+ D+GIGF+ + + K GHYG+LGI ERVR I GEI + Sbjct: 313 KHAKAENVQLKIIEESSEIKVEVIDDGIGFEVESLNKVYGHYGILGITERVRSIKGEIEI 372 Query: 377 NSNSE-GTRVKLETP 390 S GT V + P Sbjct: 373 KSKKRLGTNVSIIIP 387 >all4636 [T] COG4585 Signal transduction histidine kinase Length = 401 Score = 113 bits (282), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 68/242 (28%), Positives = 135/242 (55%), Gaps = 7/242 (2%) Query: 150 LMLIVVLAYAILFFKQVQERLRIQSFLEDLREAHEKVEELTLANERQRMARDLHDTLAQG 209 L+ +++L ++L +Q +E L + + E LR+ ++E ER R+AR++HD+L Sbjct: 161 LIFVLLLMNSLLSERQSREELAVAN--EKLRQYALRIENQATLEERNRIAREIHDSLGHS 218 Query: 210 VAGLIMRLEAADAHMSQNHPERAQEIIRQSMQQARRTLAEARRAIDNLRLKSAPEMDFKE 269 + L ++LE A ++Q++P +AQ + ++ + + L + R ++ +R ++ Sbjct: 219 LTALNLQLETA-LKLAQSNPNKAQTFLVRAKELGSKALQDVRNSVSAMRSHPLQGKTLEQ 277 Query: 270 AIADEIEHFREATGIVVSTDYRLNKRLSRLQMEHSLHIVKECLTNIARHAKADKVWVFVS 329 AI F + TGI++++ + L+ +LS I++E LTNI++HA+A +V + VS Sbjct: 278 AIDIIAADFEQNTGIILTSSFNLDYKLSTEVSTAIYRIIQESLTNISKHARATEVILEVS 337 Query: 330 TQNDKLIIEIQDNGIGFKTDDIGKDAGHYGLLGIKERVRLIGGEIYVNSN-SEGTRVKLE 388 L + I+DNG GF DI ++ +GL +++R +GG+ +NS +G ++ ++ Sbjct: 338 LNRGSLSLIIRDNGRGF---DIQQNTTGFGLQSMRDRTLALGGKFAINSAVGDGCQITVD 394 Query: 389 TP 390 P Sbjct: 395 IP 396 >CAC2940 [T] COG4585 Signal transduction histidine kinase Length = 402 Score = 95.5 bits (236), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 80/271 (29%), Positives = 133/271 (49%), Gaps = 43/271 (15%) Query: 147 IFLLMLIVVLAYAILFFKQVQER-LRIQSFLEDLREAHEK--------------VEELTL 191 +F ++LI L + F++ Q+R + Q + LRE+ EK VEEL++ Sbjct: 136 LFSILLIFTLVFIGDSFREEQKRKIEAQGLYDRLRESEEKLRGAYEKLERYSETVEELSI 195 Query: 192 ANERQRMARDLHDTLAQGVAGLIMRLEA------ADAHMSQNHPERAQEIIRQSMQQARR 245 ER R++R++HD++ ++ ++M+L+A +D S E +R ++ RR Sbjct: 196 LRERNRISREIHDSVGHTLSAVLMQLQALLYLIKSDKEESLKTVEEMTGFVRGGIENVRR 255 Query: 246 TLAEARRAIDNLRLKSAPEMDFKEAIADEIEHFREATGIVVSTDYR--LNKRLSRLQMEH 303 T+ E + D R + AI + I +++ T S D R L+K L + Sbjct: 256 TVRELK-PTDFDRFEGVF------AIEEMIRKYKKLT----SADIRFILSKEKRSLSSDE 304 Query: 304 SL---HIVKECLTNIARHAKADKVWVFVSTQNDKLIIEIQDNGIGFKTDDIGKDAGHYGL 360 S +V+E L N RH KA V V + D+L I I+DNG+G TD I K +G+ Sbjct: 305 SFISYRVVQEALNNAIRHGKASLVEVSMQFNQDRLYIRIKDNGVG--TDGIKKG---FGM 359 Query: 361 LGIKERVRLIGGEI-YVNSNSEGTRVKLETP 390 G++ERV+ +GG I + + +G V + P Sbjct: 360 TGMEERVKKLGGNIEFYSERDKGFEVTVNLP 390 >alr2137_3 [T] COG4585 Signal transduction histidine kinase Length = 210 Score = 89.0 bits (219), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 66/212 (31%), Positives = 108/212 (50%), Gaps = 19/212 (8%) Query: 184 EKVEELTLANERQRMARDLHDTLAQGVAGLIMRLEAA------DAHMSQNHPERAQEIIR 237 ++ E ++ ER RMAR++HDTLAQ G++ ++ AA D +++H + +E+ Sbjct: 1 KRAEAASILEERNRMAREIHDTLAQAFTGILAQVGAAKQVLTDDLEATESHLDLIKEL-- 58 Query: 238 QSMQQARRTLAEARRAIDNLRLKSAPEMDFKEAIADEIEHFREATGIVVSTDYRLNKRLS 297 AR L EARR++ LR + E + A+ + R A I + Y + + Sbjct: 59 -----ARTGLVEARRSVVALRPQLLEEGSLQSALHRLVAQLRTAA-IDTTLYYEIEGAVY 112 Query: 298 RLQME---HSLHIVKECLTNIARHAKADKVWVFVSTQNDKLIIEIQDNGIGFKTDDIGKD 354 L E + L + +E LTN RHA AD++ V + D+ + ++DNG GF I Sbjct: 113 SLPTEIESNLLRMGQEALTNAIRHANADEIRVELVYDRDRFCLRVRDNGQGFGVGSIPAS 172 Query: 355 AGHYGLLGIKERVRLIGGEIYVNSN-SEGTRV 385 G +GLLG+ ER IG ++ + S +GT + Sbjct: 173 EG-FGLLGMSERAERIGAQLTIRSQPGQGTEI 203 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.139 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,917,197 Number of extensions: 976003 Number of successful extensions: 3678 Number of sequences better than 1.0e-05: 66 Number of HSP's gapped: 3587 Number of HSP's successfully gapped: 66 Length of query: 396 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 288 Effective length of database: 25,922,359 Effective search space: 7465639392 Effective search space used: 7465639392 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)