BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0071.p (160 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1570 [O] COG0386 Glutathione peroxidase 224 4e-59 RSc2674 [O] COG0386 Glutathione peroxidase 209 1e-54 PA2826 [O] COG0386 Glutathione peroxidase 205 2e-53 slr1171 [O] COG0386 Glutathione peroxidase 204 4e-53 CAC1549 [O] COG0386 Glutathione peroxidase 200 9e-52 >CAC1570 [O] COG0386 Glutathione peroxidase Length = 159 Score = 224 bits (571), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 105/160 (65%), Positives = 131/160 (81%), Gaps = 1/160 (0%) Query: 1 MSIYDYEANTLRGQEESLSKYKGKVLLVVNTASKCGFTPQYKGLQEVYDKFKDRGFEVLG 60 MS+YD++A + G+E SLSKY+GKVLL+VNTASKCGFTPQYK L+++Y K + FE+LG Sbjct: 1 MSVYDFKAKDISGEEISLSKYEGKVLLIVNTASKCGFTPQYKELEDIYKKLGNEKFEILG 60 Query: 61 FPSNQFAGQEPGESEDIAEFCEINYGVTFPMYEKIDVKGDGAHPLFKYLSKEAPGVLGSK 120 FP NQFA QEPG S DI FCEINYGVTFP++EKIDVKG+ AHPLFKYL+ +A G+LG K Sbjct: 61 FPCNQFANQEPGGSGDIKNFCEINYGVTFPLFEKIDVKGENAHPLFKYLASQAGGILG-K 119 Query: 121 SIKWNFTKFLVDQEGRVLKRFAPQTTPDQIEADIAKLLEQ 160 IKWNFTKFL+D++G V+ RFAP T P +I+ I KL+E+ Sbjct: 120 EIKWNFTKFLIDKKGDVVDRFAPVTKPSKIKDKIVKLMEE 159 >RSc2674 [O] COG0386 Glutathione peroxidase Length = 165 Score = 209 bits (533), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 94/158 (59%), Positives = 124/158 (78%) Query: 3 IYDYEANTLRGQEESLSKYKGKVLLVVNTASKCGFTPQYKGLQEVYDKFKDRGFEVLGFP 62 IY +EA++L GQ LS+Y+GKVLL+VNTAS+CGFTPQY GL+ VY + ++G EVLGFP Sbjct: 4 IYAFEADSLAGQRVPLSQYRGKVLLIVNTASQCGFTPQYAGLEAVYKRLNEKGLEVLGFP 63 Query: 63 SNQFAGQEPGESEDIAEFCEINYGVTFPMYEKIDVKGDGAHPLFKYLSKEAPGVLGSKSI 122 NQF QEPG +E+I FCE NYGV+FPM+ KI+V G AHPL+K+L+ E PGVLG+++I Sbjct: 64 CNQFGKQEPGGAEEIGAFCEKNYGVSFPMFGKIEVNGSNAHPLYKWLTSEKPGVLGTEAI 123 Query: 123 KWNFTKFLVDQEGRVLKRFAPQTTPDQIEADIAKLLEQ 160 KWNFTKFL+ ++G V KR+AP T P+++ DI LL + Sbjct: 124 KWNFTKFLLRRDGTVYKRYAPLTKPEEMLGDIETLLAE 161 >PA2826 [O] COG0386 Glutathione peroxidase Length = 161 Score = 205 bits (521), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 88/158 (55%), Positives = 126/158 (79%) Query: 2 SIYDYEANTLRGQEESLSKYKGKVLLVVNTASKCGFTPQYKGLQEVYDKFKDRGFEVLGF 61 S+ T++G++++L+ + GK LLVVNTASKCGFTPQY+GL+ +++K+++RG VLGF Sbjct: 4 SLLSIPCTTIKGEQKTLADFGGKALLVVNTASKCGFTPQYQGLEALWEKYRERGLVVLGF 63 Query: 62 PSNQFAGQEPGESEDIAEFCEINYGVTFPMYEKIDVKGDGAHPLFKYLSKEAPGVLGSKS 121 P NQF QEPG+ +I++FCE+NYGV+FP++ KI+V G GAHPLF L K APG+LGS+ Sbjct: 64 PCNQFGKQEPGDEGEISQFCELNYGVSFPLFRKIEVNGAGAHPLFVSLKKRAPGLLGSQG 123 Query: 122 IKWNFTKFLVDQEGRVLKRFAPQTTPDQIEADIAKLLE 159 IKWNFTKFL+ ++G+V+KR+AP T P+++ + I LLE Sbjct: 124 IKWNFTKFLIGRDGQVVKRYAPTTKPEELSSAIEALLE 161 >slr1171 [O] COG0386 Glutathione peroxidase Length = 169 Score = 204 bits (520), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 94/162 (58%), Positives = 122/162 (75%), Gaps = 5/162 (3%) Query: 2 SIYDYEANTLRGQEESLSKYKGKVLLVVNTASKCGFTPQYKGLQEVYDKFKDRGFEVLGF 61 +IY + AN L G +L ++GKVLL+VNTAS+CGFTPQY+GLQ +Y++F DRGF VLGF Sbjct: 8 TIYGFSANALDGSPVALRDFEGKVLLIVNTASQCGFTPQYQGLQALYNRFGDRGFTVLGF 67 Query: 62 PSNQFAGQEPGESEDIAEFCEINYGVTFPMYEKIDVKGDGAHPLFKYLSKEAPGVL---- 117 P NQF QEPG S +I FCE YGVTFP++EK++V G AHPLFK+L+ +PG+ Sbjct: 68 PCNQFGQQEPGGSGEIKNFCETRYGVTFPLFEKVEVNGPNAHPLFKFLTAASPGMAIPFL 127 Query: 118 -GSKSIKWNFTKFLVDQEGRVLKRFAPQTTPDQIEADIAKLL 158 G++ IKWNFTKFLVD++G+V+KR+ PD+I ADI KLL Sbjct: 128 GGAEDIKWNFTKFLVDRQGKVVKRYGSIAKPDEIAADIEKLL 169 >CAC1549 [O] COG0386 Glutathione peroxidase Length = 181 Score = 200 bits (508), Expect = 9e-52, Method: Compositional matrix adjust. Identities = 92/181 (50%), Positives = 126/181 (69%), Gaps = 23/181 (12%) Query: 1 MSIYDYEANTLRGQEESLSKYKGKVLLVVNTASKCGFTPQYKGLQEVYDKFKDRGFEVLG 60 MS+YD++A T+ G+E SL YKGKVL++ NTASKCGFTPQY+GL+++Y ++KD+G E+LG Sbjct: 1 MSVYDFKAKTIEGKEVSLDTYKGKVLIIANTASKCGFTPQYEGLEKLYKEYKDKGLEILG 60 Query: 61 FPSNQFAGQEPGESEDIAEFCEINYGVTFPMYEKIDVKGDGAHPLFKYLSKEA------- 113 FPSNQFA QEPG++ ++ FC++NYGVTF ++EK DV+ + AHPLF YL++ A Sbjct: 61 FPSNQFAEQEPGDNNEVKNFCKLNYGVTFQLFEKTDVRDENAHPLFNYLTENAPFKGFDL 120 Query: 114 ----------------PGVLGSKSIKWNFTKFLVDQEGRVLKRFAPQTTPDQIEADIAKL 157 P L S+KWNFTKFL+D++G V+KRF P + P + DI KL Sbjct: 121 NHPTGKMLSEVLEKNFPKFLEGNSVKWNFTKFLIDRDGNVVKRFEPTSEPSDMVKDIEKL 180 Query: 158 L 158 L Sbjct: 181 L 181 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.137 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 12,720,776 Number of extensions: 545728 Number of successful extensions: 743 Number of sequences better than 1.0e-05: 18 Number of HSP's gapped: 735 Number of HSP's successfully gapped: 18 Length of query: 160 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 62 Effective length of database: 27,075,699 Effective search space: 1678693338 Effective search space used: 1678693338 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 111 (47.4 bits)