BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0065.p (410 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMa1568 [U] COG4962 Flp pilus assembly protein, ATPase CpaF 309 4e-84 RSc0652 [U] COG4962 Flp pilus assembly protein, ATPase CpaF 308 1e-83 SMc02820 [U] COG4962 Flp pilus assembly protein, ATPase CpaF 301 2e-81 AGc3735 [S] COG3238 Uncharacterized protein conserved in bacteria 300 3e-81 AGc3739 [Q] COG1335 Amidases related to nicotinamidase 300 3e-81 >SMa1568 [U] COG4962 Flp pilus assembly protein, ATPase CpaF Length = 497 Score = 309 bits (792), Expect = 4e-84, Method: Compositional matrix adjust. Identities = 166/374 (44%), Positives = 250/374 (66%), Gaps = 15/374 (4%) Query: 2 NEEMFRLLRSDIRSGLDLT---SAVGN-------RELTAYIEQTVLARDSLRHLTAQEKH 51 N E + L+ +I S L T +A+ N E+ A I V A+ + ++ E++ Sbjct: 69 NAEQYYSLKKEIFSALIATIDVAALSNMDGEQARNEIGAIINDIVAAKKA--GISMAEQN 126 Query: 52 TLVKKLFDSFRXXXXLQPLVDNPAITEIMINSHDEIFVEENGLIRRLPLAFESSSRLEDI 111 L+ + + L+PL+ I +IM+N +++F+E NG ++ + F + +L +I Sbjct: 127 DLLSDICNDILGYGPLEPLLARDDIADIMVNGANQVFIEVNGRVQETGIRFRDNEQLLNI 186 Query: 112 IQSVVSGVNRVVNDSSPIVDARLKDGSRVNIVLPPVALKGPAMTIRKFPETPMTMNELIR 171 Q +VS V R V++SSPI DARL DGSRVN++ PP+A+ GP +TIRKF + +T+++L+R Sbjct: 187 CQRIVSQVGRRVDESSPICDARLGDGSRVNVIAPPLAIDGPTLTIRKFKKEKLTLDQLVR 246 Query: 172 RETVSMEAAELLQMLVAAKYNIFISGGTGSGKTTFLNALSQFIPPQERVITIEDSAELQI 231 ++S E AE+L+++ + N+ ISGGTGSGKTT LN L+ +I ERVIT ED+AELQ+ Sbjct: 247 FGSISPEGAEVLKIIGRVRCNVLISGGTGSGKTTLLNCLTGYIDHGERVITCEDAAELQL 306 Query: 232 VTVPNLVSLETRNANTEGRGEISIRDLIRSSLRMRPNRIVVGEVRGAECLDMLQAMNTGH 291 P++V LETR N EG+GEI++R+L+++ LRMRP RI+VGEVRG E D+LQAMNTGH Sbjct: 307 -QQPHVVRLETRPPNIEGQGEITMRNLVKNCLRMRPERIIVGEVRGPEAFDLLQAMNTGH 365 Query: 292 DGSLSTGHSNSARDMVSRLETMV-LSAADLPVAVVRQQIGSAIDIFVHLSRLRDRSRRVT 350 DGS+ T H+NS R+ ++R+E M+ + + LP +R+ + S++D+ V +RLRD SRR+T Sbjct: 366 DGSMGTLHANSPREAMARIEAMITMGGSSLPAKTIREMLVSSVDVIVQAARLRDGSRRIT 425 Query: 351 EICEVAGVKEGEVV 364 I EV G+ EGEVV Sbjct: 426 HITEVLGM-EGEVV 438 >RSc0652 [U] COG4962 Flp pilus assembly protein, ATPase CpaF Length = 453 Score = 308 bits (789), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 161/355 (45%), Positives = 243/355 (68%), Gaps = 6/355 (1%) Query: 19 LTSAVGNRELTAYIEQTVLARDSLRHLTAQEKHTLVKKLFDSFRXXXXLQPLVDNPAITE 78 LT REL +E+ V + + E+H LV + D L+PL+ +P I++ Sbjct: 55 LTPEQVRRELAQLVERIV--EEGNHPVNEAERHRLVSDVQDEMLGYGPLEPLLADPTISD 112 Query: 79 IMINSHDEIFVEENGLIRRLPLAFESSSRLEDIIQSVVSGVNRVVNDSSPIVDARLKDGS 138 I++N+ +++VE G + + F + + L +I+ +VS + R +++SSP+VDARL DGS Sbjct: 113 ILVNTCKQVYVERRGKLELTDVTFHNDAHLMRVIEKMVSRMGRRIDESSPMVDARLPDGS 172 Query: 139 RVNIVLPPVALKGPAMTIRKFPETPMTMNELIRRETVSMEAAELLQMLVAAKYNIFISGG 198 R+N ++PP A+ GP ++IR+F P+T+ +LI T++ + A+LL+ +V AK NI ISGG Sbjct: 173 RINAIIPPSAIDGPLLSIRRFAVNPLTVKDLIEYNTLTPQMAQLLEAMVQAKLNILISGG 232 Query: 199 TGSGKTTFLNALSQFIPPQERVITIEDSAELQIVTVPNLVSLETRNANTEGRGEISIRDL 258 TGSGKTT LN LS +IP ERV+TIED+AELQ+ P+++ LETR N EGRGEI+ R L Sbjct: 233 TGSGKTTLLNILSGYIPVDERVVTIEDAAELQLRQ-PHVLRLETRPPNIEGRGEITQRSL 291 Query: 259 IRSSLRMRPNRIVVGEVRGAECLDMLQAMNTGHDGSLSTGHSNSARDMVSRLETMV-LSA 317 ++++LRMRP+RI++GEVRGAE LDML AMNTGH+GSL+T H+N+ RD ++RLE MV +S Sbjct: 292 VKNALRMRPDRIILGEVRGAEALDMLHAMNTGHEGSLATIHANTPRDALTRLENMVSMSG 351 Query: 318 ADLPVAVVRQQIGSAIDIFVHLSRLRDRSRRVTEICEVAGVKEGEVV-LNPLYEF 371 LP +RQQI SA+ + V ++RL D R++ + E+ G+ EG++V + ++ F Sbjct: 352 LQLPTKTMRQQITSALSVVVQVTRLTDGRRKLVSVQEITGM-EGDIVNMQEIFTF 405 >SMc02820 [U] COG4962 Flp pilus assembly protein, ATPase CpaF Length = 466 Score = 301 bits (770), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 167/378 (44%), Positives = 244/378 (64%), Gaps = 9/378 (2%) Query: 15 SGLDLTSAVGNRELTAYIEQTVLARDSLRHLTAQEKHTLVKKLFDSFRXXXXLQPLVDNP 74 S LD+ SA E+ + + ++ ++ QE+ L+ + + L+PL+ Sbjct: 65 SKLDIESA--REEIRDIVNDIITIKNFAMSISEQEE--LLDDICNDVLGYGPLEPLLARD 120 Query: 75 AITEIMINSHDEIFVEENGLIRRLPLAFESSSRLEDIIQSVVSGVNRVVNDSSPIVDARL 134 I +IM+N +IF+E G + + F + +L I Q +VS V R V++SSPI DARL Sbjct: 121 DIADIMVNGAGQIFIEVGGKVEESEIRFRDNGQLLSICQRIVSQVGRRVDESSPICDARL 180 Query: 135 KDGSRVNIVLPPVALKGPAMTIRKFPETPMTMNELIRRETVSMEAAELLQMLVAAKYNIF 194 DGSRVN++ PP+A+ G A+TIRKF + +T+ +L+R +++ EAA LLQ++ + NI Sbjct: 181 PDGSRVNVIAPPLAIDGTALTIRKFKKDKLTLEQLVRFGSITPEAAVLLQIIGRVRCNIV 240 Query: 195 ISGGTGSGKTTFLNALSQFIPPQERVITIEDSAELQIVTVPNLVSLETRNANTEGRGEIS 254 ISGGTGSGKTT LN L+++I ER+IT EDSAELQ+ P++V LETR N EG GEI+ Sbjct: 241 ISGGTGSGKTTLLNCLTRYIDSTERIITCEDSAELQL-QQPHVVRLETRPPNIEGEGEIT 299 Query: 255 IRDLIRSSLRMRPNRIVVGEVRGAECLDMLQAMNTGHDGSLSTGHSNSARDMVSRLETMV 314 +RDL+++ LRMRP RI+VGEVRG E D+LQAMNTGHDGS+ T H+N+ R+ +SR+E+M+ Sbjct: 300 MRDLVKNCLRMRPERIIVGEVRGPEVFDLLQAMNTGHDGSMGTIHANTPRECLSRMESMI 359 Query: 315 -LSAADLPVAVVRQQIGSAIDIFVHLSRLRDRSRRVTEICEVAGVKEGEVVLNPLYEFRE 373 + LP VR+ I ++D+ + SRLRD SRR+T I EV G+ EG+V++ E Sbjct: 360 AMGGYTLPARTVREIISGSVDVIIQASRLRDGSRRITHITEVTGM-EGDVIITQDLMRYE 418 Query: 374 TGGTD--GHVQGGLIPSG 389 G D G + G + +G Sbjct: 419 IDGEDANGRIVGRHVSTG 436 >AGc3735 [S] COG3238 Uncharacterized protein conserved in bacteria Length = 289 Score = 300 bits (768), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 0/343 (0%), Positives = 0/343 (0%), Gaps = 5/343 (1%) Query: 45 LTAQEKHTLVKKLFDSFRXXXXLQPLVDNPAITEIMINSHDEIFVEENGLIRRLPLAFES 104 P + +E + ++ Sbjct: 65 KPGAAVGGNATIPHKEAAYRLADHPDALAEELGAANTIWMEEGKLHATNTDGYGFVSNLD 124 Query: 105 SSRLEDIIQSVVSGVNRVVNDSSPIVDARLKDGSRVNIVLPPVALKGPAMTIRKFPETPM 164 + I R +D + +++V V Sbjct: 125 ERHPGWDKTQRAVVFGAGGASRAVIQSLRDRDVAEIHVVNRTVERARELADRFGPRVFSH 184 Query: 165 TMNELIRRETVSMEAAELLQMLVAAKYNIFISGGTGSGKTTFLNALSQFIPPQERVITIE 224 L + + + + + + + + + E Sbjct: 185 PQAALQEVMHGAGLFVNTTSLGMNGTEAPHLDFSHLAANAVVTDIVYVPLKTPILNMAQE 244 Query: 225 DSAELQIVTVPNLVSLETRNANTEGRGEISIRDLIRSSLRMRPNRIVVGEVRGAECLDML 284 Sbjct: 245 QGIATVD- 303 Query: 285 QAMNTGHDGSLSTGHSNSARDMVSRLETMV-LSAADLPVAVVRQQIGSAIDIFVHLSRLR 343 Sbjct: 304 363 Query: 344 DRSRRVTEICEVAGVKEGEVVLNPLYEFRETGGTDGHVQGGLI 386 Sbjct: 364 --- 403 >AGc3739 [Q] COG1335 Amidases related to nicotinamidase Length = 289 Score = 300 bits (768), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 0/343 (0%), Positives = 0/343 (0%), Gaps = 5/343 (1%) Query: 45 LTAQEKHTLVKKLFDSFRXXXXLQPLVDNPAITEIMINSHDEIFVEENGLIRRLPLAFES 104 P + +E + ++ Sbjct: 65 KPGAAVGGNATIPHKEAAYRLADHPDALAEELGAANTIWMEEGKLHATNTDGYGFVSNLD 124 Query: 105 SSRLEDIIQSVVSGVNRVVNDSSPIVDARLKDGSRVNIVLPPVALKGPAMTIRKFPETPM 164 + I R +D + +++V V Sbjct: 125 ERHPGWDKTQRAVVFGAGGASRAVIQSLRDRDVAEIHVVNRTVERARELADRFGPRVFSH 184 Query: 165 TMNELIRRETVSMEAAELLQMLVAAKYNIFISGGTGSGKTTFLNALSQFIPPQERVITIE 224 L + + + + + + + + + E Sbjct: 185 PQAALQEVMHGAGLFVNTTSLGMNGTEAPHLDFSHLAANAVVTDIVYVPLKTPILNMAQE 244 Query: 225 DSAELQIVTVPNLVSLETRNANTEGRGEISIRDLIRSSLRMRPNRIVVGEVRGAECLDML 284 Sbjct: 245 QGIATVD- 303 Query: 285 QAMNTGHDGSLSTGHSNSARDMVSRLETMV-LSAADLPVAVVRQQIGSAIDIFVHLSRLR 343 Sbjct: 304 363 Query: 344 DRSRRVTEICEVAGVKEGEVVLNPLYEFRETGGTDGHVQGGLI 386 Sbjct: 364 --- 403 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,087,263 Number of extensions: 1102017 Number of successful extensions: 5328 Number of sequences better than 1.0e-05: 85 Number of HSP's gapped: 5218 Number of HSP's successfully gapped: 85 Length of query: 410 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 301 Effective length of database: 25,807,025 Effective search space: 7767914525 Effective search space used: 7767914525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)