BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0050.p (514 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp1085 [U] COG4962 Flp pilus assembly protein, ATPase CpaF 70 9e-12 SMc02820 [U] COG4962 Flp pilus assembly protein, ATPase CpaF 56 1e-07 SMa1568 [U] COG4962 Flp pilus assembly protein, ATPase CpaF 54 7e-07 >RSp1085 [U] COG4962 Flp pilus assembly protein, ATPase CpaF Length = 450 Score = 70.1 bits (170), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 86/359 (23%), Positives = 154/359 (42%), Gaps = 49/359 (13%) Query: 126 AEALFHEWKGFGPAYRWFTDRAYSESTGLQMIGR-QIFYNHRGKFVVYPYEMPSLDRVEQ 184 AEAL E GFGP D A + + + G ++ + G P + + + Sbjct: 82 AEALTKELAGFGPIEDLLADPAVED---ILVNGHLDVYVSRHGVLERIPVRFADNNHLLR 138 Query: 185 LKRSLLKSDPDKKLNKDNPSVEFKMDDPLWPGRFIRLAIWVSPRVWEGFTTISLRRQVVE 244 + R +L + ++L++ NP V+ ++ D R+ + + P EG +S+R+ + Sbjct: 139 IVRRIL-APIGRRLDESNPMVDARLPDGG------RINVVIPPLALEG-PVVSIRKFRKD 190 Query: 245 FLDLEDKAGTGCIPAEAIELIRALSGTFRNTIIAGAVGSGKTTFANTVVGEQLLGSSSCM 304 L ED G G + E EL+RA N +++G SGKT+ N + + Sbjct: 191 PLKPEDLLGLGTMNEEIGELLRAAVRARCNILVSGGTSSGKTSLLNALA----YHIPTTE 246 Query: 305 GVVMIE-------KHPESI----LPYQIKGHRIIPIQAANEELMEVGVESLRHDPNILYM 353 V+ IE HP + P +G ++ I+ EL+ SLR P+ + + Sbjct: 247 RVITIEDTAELSLNHPHVVRLESRPGGFEGTGVVTIR----ELLR---NSLRMRPDRIIV 299 Query: 354 TEMRYNEWEFYLWSGEKGYDGITGTFHTVDSEDIPYQ----GAFAVSTRIGGSLKGHLIS 409 E+R E L + G+DG GT H+ + Y+ FA SL+ + + Sbjct: 300 GEVRGGEVLEMLQAMSTGHDGSMGTIHSSSPRECLYRLEMLAGFAGFQGSEVSLRRQITN 359 Query: 410 ALKSCELVFILESVADGKKRLARISEVYYDEAANSVFANDLMRWE-------EEQSAWS 461 A+ + + + +++GK+R+ ++E+ N V +L R+E EE W+ Sbjct: 360 AI---DFIVQIGRLSNGKRRILSVTEI-TGLGDNIVSTQELYRYEPYIGPDGEELDRWT 414 >SMc02820 [U] COG4962 Flp pilus assembly protein, ATPase CpaF Length = 466 Score = 56.2 bits (134), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 66/268 (24%), Positives = 123/268 (45%), Gaps = 25/268 (9%) Query: 196 KKLNKDNPSVEFKMDDPLWPGRFIRLAIWVSPRVWEGFTTISLRRQVVEFLDLEDKAGTG 255 +++++ +P + ++ D R+ + P +G T +++R+ + L LE G Sbjct: 167 RRVDESSPICDARLPD------GSRVNVIAPPLAIDG-TALTIRKFKKDKLTLEQLVRFG 219 Query: 256 CIPAEAIELIRALSGTFRNTIIAGAVGSGKTTFANTVVGEQLLGSSSCMGVVMIEKHPES 315 I EA L++ + N +I+G GSGKTT N + + + S+ ++ E E Sbjct: 220 SITPEAAVLLQIIGRVRCNIVISGGTGSGKTTLLNCLT--RYIDSTE--RIITCEDSAEL 275 Query: 316 IL--PYQIKGHRIIP-IQAANEELMEVGVES-LRHDPNILYMTEMRYNEWEFYLWSGEKG 371 L P+ ++ P I+ E M V++ LR P + + E+R E L + G Sbjct: 276 QLQQPHVVRLETRPPNIEGEGEITMRDLVKNCLRMRPERIIVGEVRGPEVFDLLQAMNTG 335 Query: 372 YDGITGTFHTVDSEDIPYQGAFAVSTRI---GGSLKGHLISALKSCELVFILES--VADG 426 +DG GT H + P + + + I G +L + + S + I+++ + DG Sbjct: 336 HDGSMGTIHA----NTPRECLSRMESMIAMGGYTLPARTVREIISGSVDVIIQASRLRDG 391 Query: 427 KKRLARISEVYYDEAANSVFANDLMRWE 454 +R+ I+EV E + + DLMR+E Sbjct: 392 SRRITHITEVTGME-GDVIITQDLMRYE 418 >SMa1568 [U] COG4962 Flp pilus assembly protein, ATPase CpaF Length = 497 Score = 53.5 bits (127), Expect = 7e-07, Method: Compositional matrix adjust. Identities = 66/274 (24%), Positives = 118/274 (43%), Gaps = 28/274 (10%) Query: 196 KKLNKDNPSVEFKMDDPLWPGRFIRLAIWVSPRVWEGFTTISLRRQVVEFLDLEDKAGTG 255 +++++ +P + ++ D R+ + P +G T+++R+ E L L+ G Sbjct: 196 RRVDESSPICDARLGDGS------RVNVIAPPLAIDG-PTLTIRKFKKEKLTLDQLVRFG 248 Query: 256 CIPAEAIELIRALSGTFRNTIIAGAVGSGKTTFANTVVGEQLLGSSSCMGVVMIEKHPES 315 I E E+++ + N +I+G GSGKTT N + G G V+ E E Sbjct: 249 SISPEGAEVLKIIGRVRCNVLISGGTGSGKTTLLNCLTGYIDHGER----VITCEDAAEL 304 Query: 316 IL--PYQIKGHRIIP-IQAANEELMEVGVES-LRHDPNILYMTEMRYNEWEFYLWSGEKG 371 L P+ ++ P I+ E M V++ LR P + + E+R E L + G Sbjct: 305 QLQQPHVVRLETRPPNIEGQGEITMRNLVKNCLRMRPERIIVGEVRGPEAFDLLQAMNTG 364 Query: 372 YDGITGTFHTVDSEDIPYQGAFAVSTRI---GGSLKGHLISAL--KSCELVFILESVADG 426 +DG GT H + P + + I G SL I + S +++ + DG Sbjct: 365 HDGSMGTLHA----NSPREAMARIEAMITMGGSSLPAKTIREMLVSSVDVIVQAARLRDG 420 Query: 427 KKRLARISEVYYDEA----ANSVFANDLMRWEEE 456 +R+ I+EV E +F D++ +E+ Sbjct: 421 SRRITHITEVLGMEGEVVTTQDLFVYDILGEDEK 454 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 39,175,004 Number of extensions: 1675319 Number of successful extensions: 3895 Number of sequences better than 1.0e-05: 3 Number of HSP's gapped: 3894 Number of HSP's successfully gapped: 3 Length of query: 514 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 403 Effective length of database: 25,576,357 Effective search space: 10307271871 Effective search space used: 10307271871 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)