BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0033.p (300 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM3248 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relat... 187 2e-47 SSO1560 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relat... 185 8e-47 ZyhaE [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related... 183 3e-46 yhaE [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related ... 183 3e-46 ZyhaE [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related... 183 3e-46 >STM3248 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Length = 296 Score = 187 bits (474), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 102/286 (35%), Positives = 160/286 (55%) Query: 3 QIGFIGLGTMGAPMASNLLRSGFEVTVYNRTASRSEPLRAEGARVAATPQHAAEGKDVII 62 ++GFIGLG MG PM+ NLL++G+ + V +R + A GA A+T + AE DVII Sbjct: 4 KVGFIGLGIMGKPMSKNLLKAGYSLVVSDRNPEAIADVIAAGAETASTAKAIAEQCDVII 63 Query: 63 TMISNDDSIREVFYGTGGILAVLKPGTTVVDSSTISPGLAKEIAAAVEERGGSFLDAPVT 122 TM+ N ++EV G GI+ KPGT ++D S+I+P ++EI+ A++ +G LDAPV+ Sbjct: 64 TMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLASREISDALKAKGVEMLDAPVS 123 Query: 123 GSKPAAIEGTLVFMVGGSAGVIDQHRDIFDSMGRLLLHMGGNGSGAVAKLAHNAMVGIHN 182 G +P AI+GTL MVGG + D++ D+ +M ++H G G+G V KLA+ +V ++ Sbjct: 124 GGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGDIGAGNVTKLANQVIVALNI 183 Query: 183 VALAEGFSIAVKSGVPADKFLELVKNGSAGSKQAELKGQKIIDNDFSNQFXXXXXXXXXX 242 A++E ++A K+GV D + ++ G AGS + K ++D +F F Sbjct: 184 AAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIKDLA 243 Query: 243 XXXXXXXXXGVPSPMLGVAKSMFQAGYNHGYGDEDLSAVVKSYEEW 288 G P+ M QA G+G++D SA+ YE+ Sbjct: 244 NALDTSHGVGAQLPLTAAVMEMMQALRADGHGNDDHSALACYYEKL 289 >SSO1560 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Length = 289 Score = 185 bits (469), Expect = 8e-47, Method: Compositional matrix adjust. Identities = 102/287 (35%), Positives = 157/287 (54%) Query: 3 QIGFIGLGTMGAPMASNLLRSGFEVTVYNRTASRSEPLRAEGARVAATPQHAAEGKDVII 62 ++GFIGLG MG PMASNLL++G+++TVYNRT ++E L GA+VA +P+ AE +++I Sbjct: 2 KVGFIGLGIMGFPMASNLLKAGYDLTVYNRTIEKAEKLGKMGAKVAHSPKEVAEVSEIVI 61 Query: 63 TMISNDDSIREVFYGTGGILAVLKPGTTVVDSSTISPGLAKEIAAAVEERGGSFLDAPVT 122 +M+++ + EV +G G++ K G VD ST SP AK++ + E G FLDAPVT Sbjct: 62 SMVTDAPDVEEVLFGENGVVKSNKRGLIFVDMSTNSPEFAKKVTKRLSEYGMEFLDAPVT 121 Query: 123 GSKPAAIEGTLVFMVGGSAGVIDQHRDIFDSMGRLLLHMGGNGSGAVAKLAHNAMVGIHN 182 G A EGTL MVGG V + IF +MG+ ++H+G GSG KL + +V ++ Sbjct: 122 GGDKGAREGTLTIMVGGKEDVFKRVEPIFKAMGKNIIHVGDVGSGQALKLCNQVVVALNM 181 Query: 183 VALAEGFSIAVKSGVPADKFLELVKNGSAGSKQAELKGQKIIDNDFSNQFXXXXXXXXXX 242 V++ EG +A G+ DK ++ G+A S + KI+ D + F Sbjct: 182 VSVVEGLLLARSLGIDDDKLFSVLSTGAANSFTVQYYLPKIMKGDLNPGFKAAHLKKDLK 241 Query: 243 XXXXXXXXXGVPSPMLGVAKSMFQAGYNHGYGDEDLSAVVKSYEEWI 289 +P +A ++ A + G G+ +VK YE+ + Sbjct: 242 YAMEIANSKSLPLLGTSLALQLYNAMVSLGIGELGTQGLVKVYEKLV 288 >ZyhaE [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Length = 299 Score = 183 bits (465), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 102/286 (35%), Positives = 157/286 (54%) Query: 3 QIGFIGLGTMGAPMASNLLRSGFEVTVYNRTASRSEPLRAEGARVAATPQHAAEGKDVII 62 ++GFIGLG MG PM+ NLL++G+ + V +R + A GA A+T + AE DVII Sbjct: 7 KVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCDVII 66 Query: 63 TMISNDDSIREVFYGTGGILAVLKPGTTVVDSSTISPGLAKEIAAAVEERGGSFLDAPVT 122 TM+ N ++EV G GI+ KPGT ++D S+I+P ++EI+ A++ +G LDAPV+ Sbjct: 67 TMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAPVS 126 Query: 123 GSKPAAIEGTLVFMVGGSAGVIDQHRDIFDSMGRLLLHMGGNGSGAVAKLAHNAMVGIHN 182 G +P AI+GTL MVGG + D++ D+ +M ++H G G+G V KLA+ +V ++ Sbjct: 127 GGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGEIGAGNVTKLANQVIVALNI 186 Query: 183 VALAEGFSIAVKSGVPADKFLELVKNGSAGSKQAELKGQKIIDNDFSNQFXXXXXXXXXX 242 A++E ++A K+GV D + ++ G AGS + K ++D +F F Sbjct: 187 AAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIKDLA 246 Query: 243 XXXXXXXXXGVPSPMLGVAKSMFQAGYNHGYGDEDLSAVVKSYEEW 288 G P+ M QA G G D SA+ YE+ Sbjct: 247 NALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKL 292 >yhaE [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Length = 299 Score = 183 bits (465), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 102/286 (35%), Positives = 157/286 (54%) Query: 3 QIGFIGLGTMGAPMASNLLRSGFEVTVYNRTASRSEPLRAEGARVAATPQHAAEGKDVII 62 ++GFIGLG MG PM+ NLL++G+ + V +R + A GA A+T + AE DVII Sbjct: 7 KVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCDVII 66 Query: 63 TMISNDDSIREVFYGTGGILAVLKPGTTVVDSSTISPGLAKEIAAAVEERGGSFLDAPVT 122 TM+ N ++EV G GI+ KPGT ++D S+I+P ++EI+ A++ +G LDAPV+ Sbjct: 67 TMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAPVS 126 Query: 123 GSKPAAIEGTLVFMVGGSAGVIDQHRDIFDSMGRLLLHMGGNGSGAVAKLAHNAMVGIHN 182 G +P AI+GTL MVGG + D++ D+ +M ++H G G+G V KLA+ +V ++ Sbjct: 127 GGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGEIGAGNVTKLANQVIVALNI 186 Query: 183 VALAEGFSIAVKSGVPADKFLELVKNGSAGSKQAELKGQKIIDNDFSNQFXXXXXXXXXX 242 A++E ++A K+GV D + ++ G AGS + K ++D +F F Sbjct: 187 AAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIKDLA 246 Query: 243 XXXXXXXXXGVPSPMLGVAKSMFQAGYNHGYGDEDLSAVVKSYEEW 288 G P+ M QA G G D SA+ YE+ Sbjct: 247 NALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKL 292 Score = 183 bits (465), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 102/286 (35%), Positives = 157/286 (54%) Query: 3 QIGFIGLGTMGAPMASNLLRSGFEVTVYNRTASRSEPLRAEGARVAATPQHAAEGKDVII 62 ++GFIGLG MG PM+ NLL++G+ + V +R + A GA A+T + AE DVII Sbjct: 7 KVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCDVII 66 Query: 63 TMISNDDSIREVFYGTGGILAVLKPGTTVVDSSTISPGLAKEIAAAVEERGGSFLDAPVT 122 TM+ N ++EV G GI+ KPGT ++D S+I+P ++EI+ A++ +G LDAPV+ Sbjct: 67 TMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAPVS 126 Query: 123 GSKPAAIEGTLVFMVGGSAGVIDQHRDIFDSMGRLLLHMGGNGSGAVAKLAHNAMVGIHN 182 G +P AI+GTL MVGG + D++ D+ +M ++H G G+G V KLA+ +V ++ Sbjct: 127 GGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGEIGAGNVTKLANQVIVALNI 186 Query: 183 VALAEGFSIAVKSGVPADKFLELVKNGSAGSKQAELKGQKIIDNDFSNQFXXXXXXXXXX 242 A++E ++A K+GV D + ++ G AGS + K ++D +F F Sbjct: 187 AAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIKDLA 246 Query: 243 XXXXXXXXXGVPSPMLGVAKSMFQAGYNHGYGDEDLSAVVKSYEEW 288 G P+ M QA G G D SA+ YE+ Sbjct: 247 NALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKL 292 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.133 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,507,351 Number of extensions: 898359 Number of successful extensions: 2757 Number of sequences better than 1.0e-05: 61 Number of HSP's gapped: 2684 Number of HSP's successfully gapped: 61 Length of query: 300 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 194 Effective length of database: 26,153,027 Effective search space: 5073687238 Effective search space used: 5073687238 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 115 (48.9 bits)