BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0016.p (382 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0602 [C] COG0371 Glycerol dehydrogenase and related enzymes 200 4e-51 ybdH [C] COG0371 Glycerol dehydrogenase and related enzymes 190 3e-48 ZybdH [C] COG0371 Glycerol dehydrogenase and related enzymes 190 3e-48 ECs0638 [C] COG0371 Glycerol dehydrogenase and related enzymes 190 3e-48 >STM0602 [C] COG0371 Glycerol dehydrogenase and related enzymes Length = 362 Score = 200 bits (508), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 130/343 (37%), Positives = 181/343 (52%), Gaps = 2/343 (0%) Query: 13 QIIVRAAPQEFICRPGSWGSLEQHLQXXXXXXXXXXXXXXSWTAAAPYWPELKETEVHYH 72 +I V P + GS L + AA PY PE E H Sbjct: 5 EIRVVTGPANYFSHAGSLERLTDFFTPEQLSHAVWVYGERAIAAARPYLPEAFERAGAKH 64 Query: 73 V-YSGECSYRERDAIAGYASEHQLEAVIAVGGGKITDLVKSAAATLNLPAIILPTLAATC 131 + ++G CS R +A +A + VI VGGG + D K+ A L LP + +PT+AATC Sbjct: 65 LPFTGHCSERHVAQLA-HACNDDRQVVIGVGGGALLDTAKALARRLALPFVAIPTIAATC 123 Query: 132 AAWSSLSVMYDERGAFIRFEVFPRSNALVLLDPVVIAASPPELLAAGIGDTLAKWYEADV 191 AAW+ LSV Y++ G ++FE+F +N LVL++P +I +P + L AGIGDTLAKWYEA V Sbjct: 124 AAWTPLSVWYNDAGQALQFEIFDDANFLVLVEPRIILQAPDDYLLAGIGDTLAKWYEAVV 183 Query: 192 IIRHLEAPPVEVELAWDAARKCRENLLRYSSAALAAVRSGELNEALTRTAETIIMVAGLV 251 + E P+ V L ++A R+ LL S ALA + L +A + II G+V Sbjct: 184 LAPQPETLPLTVRLGINSACAIRDLLLDSSEQALADKQQRRLTQAFCDVVDAIIAGGGMV 243 Query: 252 GGFGEDYGRTAGAHSVHDALTAIPETHGLLHGSKVAYGVLVQLALEGNWPEIDGLLPFYE 311 GG GE Y R A AH+VH+ LT +P+T LHG+KVAYG+LVQ AL G + L+ Y Sbjct: 244 GGLGERYTRVAAAHAVHNGLTVLPQTEKFLHGTKVAYGILVQSALLGQDDVLAQLITAYR 303 Query: 312 ELGLPSSLRAMGLGHLTREELLKLGERAAVPEASIHMMPGVIT 354 LP+ L + + E+ ++ P SIH +P +T Sbjct: 304 RFHLPARLSELDVDIHNTAEIDRVIAHTLRPVESIHYLPVTLT 346 >ybdH [C] COG0371 Glycerol dehydrogenase and related enzymes Length = 362 Score = 190 bits (483), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 123/348 (35%), Positives = 183/348 (52%), Gaps = 3/348 (0%) Query: 8 MPVEPQIIVRAAPQEFICRPGSWGSLEQHLQXXXXXXXXXXXXXXSWTAAAPYWPELKET 67 MP P I V P + PGS+ L + AA P Sbjct: 1 MPHNP-IRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKRAIAAAQTKLPPAFGL 59 Query: 68 EVHYHV-YSGECSYRERDAIAGYASEHQLEAVIAVGGGKITDLVKSAAATLNLPAIILPT 126 H+ + G CS + +A + + + VI VGGG + D K+ A L LP + +PT Sbjct: 60 PGAKHILFRGHCSESDVQQLAAESGDDR-SVVIGVGGGALLDTAKALARRLGLPFVAVPT 118 Query: 127 LAATCAAWSSLSVMYDERGAFIRFEVFPRSNALVLLDPVVIAASPPELLAAGIGDTLAKW 186 +AATCAAW+ LSV Y++ G + +E+F +N +VL++P +I +P + L AGIGDTLAKW Sbjct: 119 IAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIILNAPQQYLLAGIGDTLAKW 178 Query: 187 YEADVIIRHLEAPPVEVELAWDAARKCRENLLRYSSAALAAVRSGELNEALTRTAETIIM 246 YEA V+ E P+ V L + A+ R+ LL S AL+ ++ +L ++ + II Sbjct: 179 YEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALSDQQNQQLTQSFCDVVDAIIA 238 Query: 247 VAGLVGGFGEDYGRTAGAHSVHDALTAIPETHGLLHGSKVAYGVLVQLALEGNWPEIDGL 306 G+VGG G+ + R A AH+VH+ LT +P+T LHG+KVAYG+LVQ AL G + L Sbjct: 239 GGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGTKVAYGILVQSALLGQDDVLAQL 298 Query: 307 LPFYEELGLPSSLRAMGLGHLTREELLKLGERAAVPEASIHMMPGVIT 354 Y+ LP++L + + + E+ K+ P SIH +P +T Sbjct: 299 TGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRPVESIHYLPVTLT 346 >ZybdH [C] COG0371 Glycerol dehydrogenase and related enzymes Length = 362 Score = 190 bits (483), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 123/348 (35%), Positives = 183/348 (52%), Gaps = 3/348 (0%) Query: 8 MPVEPQIIVRAAPQEFICRPGSWGSLEQHLQXXXXXXXXXXXXXXSWTAAAPYWPELKET 67 MP P I V P + PGS+ L + AA P Sbjct: 1 MPHNP-IRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKRAIAAAQTKLPPAFGL 59 Query: 68 EVHYHV-YSGECSYRERDAIAGYASEHQLEAVIAVGGGKITDLVKSAAATLNLPAIILPT 126 H+ + G CS + +A + + + VI VGGG + D K+ A L LP + +PT Sbjct: 60 PGAKHILFRGHCSESDVQQLAAESGDDR-SVVIGVGGGALLDTAKALARRLGLPFVAVPT 118 Query: 127 LAATCAAWSSLSVMYDERGAFIRFEVFPRSNALVLLDPVVIAASPPELLAAGIGDTLAKW 186 +AATCAAW+ LSV Y++ G + +E+F +N +VL++P +I +P + L AGIGDTLAKW Sbjct: 119 IAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIILNAPQQYLLAGIGDTLAKW 178 Query: 187 YEADVIIRHLEAPPVEVELAWDAARKCRENLLRYSSAALAAVRSGELNEALTRTAETIIM 246 YEA V+ E P+ V L + A+ R+ LL S AL+ ++ +L ++ + II Sbjct: 179 YEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALSDQQNQQLTQSFCDVVDAIIA 238 Query: 247 VAGLVGGFGEDYGRTAGAHSVHDALTAIPETHGLLHGSKVAYGVLVQLALEGNWPEIDGL 306 G+VGG G+ + R A AH+VH+ LT +P+T LHG+KVAYG+LVQ AL G + L Sbjct: 239 GGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGTKVAYGILVQSALLGQDDVLAQL 298 Query: 307 LPFYEELGLPSSLRAMGLGHLTREELLKLGERAAVPEASIHMMPGVIT 354 Y+ LP++L + + + E+ K+ P SIH +P +T Sbjct: 299 TGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRPVESIHYLPVTLT 346 Score = 190 bits (483), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 123/348 (35%), Positives = 183/348 (52%), Gaps = 3/348 (0%) Query: 8 MPVEPQIIVRAAPQEFICRPGSWGSLEQHLQXXXXXXXXXXXXXXSWTAAAPYWPELKET 67 MP P I V P + PGS+ L + AA P Sbjct: 1 MPHNP-IRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKRAIAAAQTKLPPAFGL 59 Query: 68 EVHYHV-YSGECSYRERDAIAGYASEHQLEAVIAVGGGKITDLVKSAAATLNLPAIILPT 126 H+ + G CS + +A + + + VI VGGG + D K+ A L LP + +PT Sbjct: 60 PGAKHILFRGHCSESDVQQLAAESGDDR-SVVIGVGGGALLDTAKALARRLGLPFVAVPT 118 Query: 127 LAATCAAWSSLSVMYDERGAFIRFEVFPRSNALVLLDPVVIAASPPELLAAGIGDTLAKW 186 +AATCAAW+ LSV Y++ G + +E+F +N +VL++P +I +P + L AGIGDTLAKW Sbjct: 119 IAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIILNAPQQYLLAGIGDTLAKW 178 Query: 187 YEADVIIRHLEAPPVEVELAWDAARKCRENLLRYSSAALAAVRSGELNEALTRTAETIIM 246 YEA V+ E P+ V L + A+ R+ LL S AL+ ++ +L ++ + II Sbjct: 179 YEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALSDQQNQQLTQSFCDVVDAIIA 238 Query: 247 VAGLVGGFGEDYGRTAGAHSVHDALTAIPETHGLLHGSKVAYGVLVQLALEGNWPEIDGL 306 G+VGG G+ + R A AH+VH+ LT +P+T LHG+KVAYG+LVQ AL G + L Sbjct: 239 GGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGTKVAYGILVQSALLGQDDVLAQL 298 Query: 307 LPFYEELGLPSSLRAMGLGHLTREELLKLGERAAVPEASIHMMPGVIT 354 Y+ LP++L + + + E+ K+ P SIH +P +T Sbjct: 299 TGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRPVESIHYLPVTLT 346 >ECs0638 [C] COG0371 Glycerol dehydrogenase and related enzymes Length = 362 Score = 190 bits (483), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 124/348 (35%), Positives = 182/348 (52%), Gaps = 3/348 (0%) Query: 8 MPVEPQIIVRAAPQEFICRPGSWGSLEQHLQXXXXXXXXXXXXXXSWTAAAPYWPELKET 67 MP P I V P + PGS+ L + AA P Sbjct: 1 MPHNP-IRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGVRAIAAAQTKLPPAFGL 59 Query: 68 EVHYHV-YSGECSYRERDAIAGYASEHQLEAVIAVGGGKITDLVKSAAATLNLPAIILPT 126 H+ + G CS + +A + + + VI VGGG + D K+ A L LP + PT Sbjct: 60 PGAKHILFRGHCSESDVQQLAAESGDDR-SVVIGVGGGALLDTAKALARRLGLPFVAAPT 118 Query: 127 LAATCAAWSSLSVMYDERGAFIRFEVFPRSNALVLLDPVVIAASPPELLAAGIGDTLAKW 186 +AATCAAW+ LSV Y++ G + +E+F +N +VL++P +I +P + L AGIGDTLAKW Sbjct: 119 IAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIILNAPQQYLLAGIGDTLAKW 178 Query: 187 YEADVIIRHLEAPPVEVELAWDAARKCRENLLRYSSAALAAVRSGELNEALTRTAETIIM 246 YEA V+ E P+ V L + A+ R+ LL S ALA ++ +L ++ + II Sbjct: 179 YEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALADQQNQQLTQSFCDVVDAIIA 238 Query: 247 VAGLVGGFGEDYGRTAGAHSVHDALTAIPETHGLLHGSKVAYGVLVQLALEGNWPEIDGL 306 G+VGG G+ + R A AH+VH+ LT +P+T LHG+KVAYG+LVQ AL G + L Sbjct: 239 GGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGTKVAYGILVQSALLGQDDVLAQL 298 Query: 307 LPFYEELGLPSSLRAMGLGHLTREELLKLGERAAVPEASIHMMPGVIT 354 Y+ LP++L + + + E+ K+ P SIH +P +T Sbjct: 299 TGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRPVESIHYLPVTLT 346 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,906,556 Number of extensions: 943633 Number of successful extensions: 2115 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 2096 Number of HSP's successfully gapped: 15 Length of query: 382 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 274 Effective length of database: 25,922,359 Effective search space: 7102726366 Effective search space used: 7102726366 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)