BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0007.p (267 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM3831 [R] COG0561 Predicted hydrolases of the HAD superfamily 136 4e-32 VC1364 [R] COG0561 Predicted hydrolases of the HAD superfamily 136 4e-32 yidA [R] COG0561 Predicted hydrolases of the HAD superfamily 135 9e-32 ZyidA [R] COG0561 Predicted hydrolases of the HAD superfamily 135 9e-32 >STM3831 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 281 Score = 136 bits (342), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 90/270 (33%), Positives = 139/270 (51%), Gaps = 11/270 (4%) Query: 3 KLIAIDIDDTLINDDKEVTPATQTALEQXXXXXXXXXXXXXRAYASAQAIARQTGLNVP- 61 KLIAID+D TL+ D ++PA + A+ R YA + ++ + P Sbjct: 4 KLIAIDMDGTLLLPDHTISPAVKNAIAAAREKGVNVVLTTGRPYAGVHSYLKELHMEQPG 63 Query: 62 --IITYQGALVKNLMDEKVLYERYVPQDAVRKLFNYCVEHNLHLQTYIDDKLYARDENQK 119 ITY GALV+ D + + + D R L E H + LY + + Sbjct: 64 DYCITYNGALVQKAGDGSTVAQTALSYDDYRYLEKLSREVGSHFHALDRNTLYTANRD-- 121 Query: 120 LVDYATLNGTKYYIEP----DWEKLVPQ-KTPKMLIIDDPDFLDELSPILRELLGDSVHI 174 + Y T++ + P + EK+ P + K+++ID+P LD + + + + Sbjct: 122 -ISYYTVHESYVATIPLVFCEAEKMDPNTQFLKVMMIDEPAVLDRAIARIPAEVKEKYTV 180 Query: 175 TKSKPHFLEIMHKEGTKGLALEFLAAHFGCELSETIAVGDSWNDHEMLEAAGLGVAMANA 234 KS P+FLEI+ K KG ++ L G + E +A+GD ND M+E AG+GVAM NA Sbjct: 181 LKSAPYFLEILDKRVNKGTGVKSLVEALGIKPEEVMAIGDQENDIAMIEYAGMGVAMDNA 240 Query: 235 IPALKDIADFVTLSNNEDGVKYAIDKFILQ 264 IP++K++A+FVT SN EDGV +AI+KF+L Sbjct: 241 IPSVKEVANFVTKSNLEDGVAWAIEKFVLN 270 >VC1364 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 273 Score = 136 bits (342), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 83/268 (30%), Positives = 140/268 (52%), Gaps = 6/268 (2%) Query: 1 MYKLIAIDIDDTLINDDKEVTPATQTALEQXXXXXXXXXXXXXRAYASAQAIARQTGLNV 60 MYKLIA+D+D TL+N K+++P T+ A+ Q R ++ + L Sbjct: 1 MYKLIALDMDGTLLNSQKQISPRTKQAIAQARQQGIQVVLASGRPLDGMRSKLEELHLTT 60 Query: 61 P---IITYQGALVKNLMDEKVLYERYVPQDAVRKLFNYCVEHNLHLQTYID-DKLYARDE 116 ++ Y G++V N+ ++++++ + A +++ E L+ + L Sbjct: 61 ENDYVLYYNGSMVANVGTGEIIHQQIIDGKAAKRVATLAQEFGLNTHAFSQIHGLITPKT 120 Query: 117 NQKLVDYATLNGTKYYIEPDWEKLVP-QKTPKMLIIDDPDFLDELSPILRELLGDSVHIT 175 +Q A++NG E D+ +L K +I+ +P+ L + L + + Sbjct: 121 SQYTEVEASINGLNI-TEMDFAQLADDHPIIKAMIVGEPEKLTQAIAQLPAQWREEFTVV 179 Query: 176 KSKPHFLEIMHKEGTKGLALEFLAAHFGCELSETIAVGDSWNDHEMLEAAGLGVAMANAI 235 +S P FLE ++ KG+ ++ +AAH G + SE I +GD+ NDH ML+ AGLG+AMANA+ Sbjct: 180 QSAPFFLEFLNTGSNKGIGVQAIAAHLGIQASEVICMGDAENDHHMLQYAGLGIAMANAM 239 Query: 236 PALKDIADFVTLSNNEDGVKYAIDKFIL 263 K IAD +TLSN+ DGV AI++F L Sbjct: 240 EETKHIADHITLSNDHDGVAVAIERFAL 267 >yidA [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 270 Score = 135 bits (339), Expect = 9e-32, Method: Compositional matrix adjust. Identities = 90/270 (33%), Positives = 140/270 (51%), Gaps = 11/270 (4%) Query: 3 KLIAIDIDDTLINDDKEVTPATQTALEQXXXXXXXXXXXXXRAYASAQAIARQTGLNVP- 61 KLIAID+D TL+ D ++PA + A+ R YA ++ + P Sbjct: 4 KLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTGRPYAGVHNYLKELHMEQPG 63 Query: 62 --IITYQGALVKNLMDEKVLYERYVPQDAVRKLFNYCVEHNLHLQTYIDDKLYARDENQK 119 ITY GALV+ D + + + D R L E H LY + + Sbjct: 64 DYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSREVGSHFHALDRTTLYTANRD-- 121 Query: 120 LVDYATLNGTKYYIEP----DWEKLVPQ-KTPKMLIIDDPDFLDELSPILRELLGDSVHI 174 + Y T++ + P + EK+ P + K+++ID+P LD+ + + + + + Sbjct: 122 -ISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEPAILDQAIARIPQEVKEKYTV 180 Query: 175 TKSKPHFLEIMHKEGTKGLALEFLAAHFGCELSETIAVGDSWNDHEMLEAAGLGVAMANA 234 KS P+FLEI+ K KG ++ LA G + E +A+GD ND M+E AG+GVAM NA Sbjct: 181 LKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAIGDQENDIAMIEYAGVGVAMDNA 240 Query: 235 IPALKDIADFVTLSNNEDGVKYAIDKFILQ 264 IP++K++A+FVT SN EDGV +AI+K++L Sbjct: 241 IPSVKEVANFVTKSNLEDGVAFAIEKYVLN 270 >ZyidA [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 270 Score = 135 bits (339), Expect = 9e-32, Method: Compositional matrix adjust. Identities = 90/270 (33%), Positives = 140/270 (51%), Gaps = 11/270 (4%) Query: 3 KLIAIDIDDTLINDDKEVTPATQTALEQXXXXXXXXXXXXXRAYASAQAIARQTGLNVP- 61 KLIAID+D TL+ D ++PA + A+ R YA ++ + P Sbjct: 4 KLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTGRPYAGVHNYLKELHMEQPG 63 Query: 62 --IITYQGALVKNLMDEKVLYERYVPQDAVRKLFNYCVEHNLHLQTYIDDKLYARDENQK 119 ITY GALV+ D + + + D R L E H LY + + Sbjct: 64 DYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSREVGSHFHALDRTTLYTANRD-- 121 Query: 120 LVDYATLNGTKYYIEP----DWEKLVPQ-KTPKMLIIDDPDFLDELSPILRELLGDSVHI 174 + Y T++ + P + EK+ P + K+++ID+P LD+ + + + + + Sbjct: 122 -ISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEPAILDQAIARIPQEVKEKYTV 180 Query: 175 TKSKPHFLEIMHKEGTKGLALEFLAAHFGCELSETIAVGDSWNDHEMLEAAGLGVAMANA 234 KS P+FLEI+ K KG ++ LA G + E +A+GD ND M+E AG+GVAM NA Sbjct: 181 LKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAIGDQENDIAMIEYAGVGVAMDNA 240 Query: 235 IPALKDIADFVTLSNNEDGVKYAIDKFILQ 264 IP++K++A+FVT SN EDGV +AI+K++L Sbjct: 241 IPSVKEVANFVTKSNLEDGVAFAIEKYVLN 270 Score = 134 bits (338), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 90/270 (33%), Positives = 140/270 (51%), Gaps = 11/270 (4%) Query: 3 KLIAIDIDDTLINDDKEVTPATQTALEQXXXXXXXXXXXXXRAYASAQAIARQTGLNVP- 61 KLIAID+D TL+ D ++PA + A+ R YA ++ + P Sbjct: 4 KLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTGRPYAGVHNYLKELHMEQPG 63 Query: 62 --IITYQGALVKNLMDEKVLYERYVPQDAVRKLFNYCVEHNLHLQTYIDDKLYARDENQK 119 ITY GALV+ D + + + D R L E H LY + + Sbjct: 64 DYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSREVGSHFHALDRTTLYTANRD-- 121 Query: 120 LVDYATLNGTKYYIEP----DWEKLVPQ-KTPKMLIIDDPDFLDELSPILRELLGDSVHI 174 + Y T++ + P + EK+ P + K+++ID+P LD+ + + + + + Sbjct: 122 -ISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEPAILDQAIARIPQEVKEKYTV 180 Query: 175 TKSKPHFLEIMHKEGTKGLALEFLAAHFGCELSETIAVGDSWNDHEMLEAAGLGVAMANA 234 KS P+FLEI+ K KG ++ LA G + E +A+GD ND M+E AG+GVAM NA Sbjct: 181 LKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAIGDQENDIAMIEYAGVGVAMDNA 240 Query: 235 IPALKDIADFVTLSNNEDGVKYAIDKFILQ 264 IP++K++A+FVT SN EDGV +AI+K++L Sbjct: 241 IPSVKEVANFVTKSNLEDGVAFAIEKYVLN 270 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,227,158 Number of extensions: 760733 Number of successful extensions: 2061 Number of sequences better than 1.0e-05: 127 Number of HSP's gapped: 1959 Number of HSP's successfully gapped: 127 Length of query: 267 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 162 Effective length of database: 26,268,361 Effective search space: 4255474482 Effective search space used: 4255474482 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)